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Shiv Kumar Verma, Ph. D.

Email: ac51g4@r.postjobfree.com, ac51g4@r.postjobfree.com, Phone: 443-***-**** CONTACT DETAILS

Work Address: United States Department of Agriculture (USDA), ARS, 10300 Baltimore Avenue, BLDG 1040, BARC-EAST

Beltsville, MD 20705, USA.

Home Address: Shiv K. Verma, 11 Parkway, Apt-F

Greenbelt, MD 20770, USA.

SUMMARY OF QUALIFICATIONS

• I have over 10-years research experience in studying economically important human and veterinary pathogens.

• Experimental knowledge of many diseases cause by prokaryotes, eukaryotes, virus, and life style diseases, pathology, drug and vaccine developments, and disease managements.

• Metagenomics, Next-generation sequencing (NGS), manage and perform MiSeq, NGS data analysis; sequences alignment, assembly, phylogeny, sequence submission along with working experience in Linux environment.

• Expert in animal handling, dosing, infecting, rearing, breeding and maintenance of laboratory animals; mice, rat, jird, hamster, rabbit, chicken, cat.

• Handled many assigned projects of in vitro and in vivo screening of lead compounds in drug developments.

• Actively involved in identification of immunogenic proteins and vaccine development projects.

• Characterization of immune-responses in course of disease progression and suppression of hosts immune defense system and immune-pathology.

RELEVANT SKILLS

• DNA and RNA isolation from different samples (tissues, blood, soil, stool) and PCR, RT-PCR and real time PCR.

• Next-Generation Sequencing (NGS), Illumina library preparations, Manage and perform MiSeq run, NGS data analysis.

• Polyacrylamide gel electrophoresis (Native and reduced), elution of protein/DNA from gels.

• 2D gel electrophoresis and trypsin digestion for MALDI-TOF.

• Working research experiences (>10 years) in animal biosafety level 2(BSL2).

• In vivo and in vitro screening of chemotherapeutic agents (synthetic compounds and natural products).

• Macrophage cell culture and cell line culture –Mouse cell line (J-774, RAW 264.7), Human cell line (THP-1) and Monkey cell lineage (Vero), CV1.

• Manage and perform the in vivo animal models for drug discovery and development by validating and establishing new drug screens using new parasite strains and animal species as needed.

• Pathological studies (H-E and Toluidine blue staining) and immuno-staining.

• Cloning, expression and purification of proteins, and characterization.

• Identification and characterization of parasite proteins against different test sera and cell lysate by Western blotting.

• Estimation of cytokines against different antigens by Sandwich ELISA and cytokine bead array method.

• Antibody (IgG & subclasses, IgM and IgE) detections by ELISA.

• Immunohistochemistry for different cell surface and cytoplasmic antigens by classical methods and fluorescent microscopy.

WORK EXPERIENCES

• Visiting Scientist: USDA-ARS, Beltsville, MD 20705, USA: June 2012–Till present.

• Senior Research fellow: Central Drug Research Institute (CSIR), Lucknow, India: March 2008–Feb 2012.

• Junior Research fellow: Central Drug Research Institute (CSIR), Lucknow, India: Feb 2006 –Feb 2008. Page 2 of 3

EDUCATION

• Ph.D. Life Sciences: University - Jawaharlal Nehru University, New Delhi, India. Institution - Central Drug Research Institute, Lucknow, (U.P.) India. Area of research –Microbiology.

• Master of Science (M.Sc.): University - Banaras Hindu University, Varanasi (U.P.) India. SELECTED PUBLICATIONS

Google Scholar Profile: https://scholar.google.com/citations?user=j5nu7_IAAAAJ&hl=en

[1] Verma, S.K., Lindsay, D.S., Grigg, M.E., Dubey, J.P., 2017. Isolation, Culture and Cryopreservation of Sarcocystis species. Curr Protoc Microbiol.

[2] Verma, S.K., Knowles, S., Cerqueira-Cézar, C.K., Kwok, O.C., Jiang, T., Su, C., Dubey, J.P., 2018. An update on Toxoplasma gondii infections in northern sea otters (Enhydra lutris kenyoni) from Washington State, USA. Vet Parasitology.

[3] Verma, S.K., Sweeny, A.R., Lovallo, M.J., Calero-Bernal, R., Kwok, O.C., Jiang, T., Su, C., Grigg, M.E., Dubey, J.P., 2017. Seroprevalence, isolation, and co-infection of multiple Toxoplasma gondii strains in individual bobcats (Lynx rufus) from Mississippi, USA. Int. J. Parasitol.

[4] Verma, S.K., Minicucci, L., Murphy, D., Carstensen, M., Humpal, C., Wolf, P., Calero-Bernal, R., Cerqueira-Cezar, C.K., Kwok, O.C., Su, C., Hill, D., Dubey, J.P., 2016. Antibody Detection and Molecular Characterization of Toxoplasma gondii from Bobcats (Lynx rufus), Domestic Cats (Felis catus), and Wildlife from Minnesota, USA. J. Eukaryot. Microbiol.

[5] Verma, S.K., Calero-Bernal, R., Cerqueira-Cezar, C.K., Kwok, O.C., Dudley, M., Jiang, T., Su, C., Hill, D., Dubey, J.P., 2016. Toxoplasmosis in geese and detection of two new atypical Toxoplasma gondii strains from naturally infected Canada geese (Branta canadensis). Parasitol. Res.

[6] Verma, S.K., Cerqueira-Cezar, C.K., Murata, F.H.A., Lovallo, M.J., Rosenthal, B.M., Dubey, J.P., 2017. Bobcats (Lynx rufus) are natural definitive host of Besnoitia darlingi. Vet. Parasitol.

[7] Verma, S.K., Lindsay, D.S., Rosenthal, B.M., Dubey, J.P., 2016. Ancient, globally distributed lineage of Sarcocystis from sporocysts of the Eastern rat snake (Pantherophis alleghaniensis) and its relation to neurological sequalae in intermediate hosts. Parasitol. Res.

[8] Verma, S. K., Lindsay, D.S., Mowery, J.D., Rosenthal, B.M., Dubey, J.P., 2017. Sarcocystis pantherophisi, n. sp. from eastern rat snakes (Pantherophis alleghaniensis) definitive hosts and interferon gamma gene knockout mice as experimental intermediate hosts. J. Parasitol.

[9] Verma, S. K., Von Dohlen, A. R., Mowery, J.D., Scott, D., Cezar, C., Rosenthal, B.M., Dubey, J.P., Lindsay, D.S. 2017. Sarcocystis strixi, n. sp. from a barred owl (Strix varia) definitive host and interferon gamma gene knockout mice as experimental intermediate host. J. Parasitol.

[10] Verma, S. K., Von Dohlen, A. R., Mowery, J.D., Scott, D., Rosenthal, B.M., Dubey, J.P., Lindsay, D.S. 2017. Sarcocystis jamaicensis, n. sp. from red-tailed hawks (Buteo jamaicensis) definitive host and IFN-γ gene knockout mice as experimental intermediate host. J. Parasitol.

[11] Calero-Bernal, R.*, Verma, S.K.*, Oliveira, S., Yang, Y., Rosenthal, B.M., Dubey, J.P., 2015. In the United States, negligible rates of zoonotic sarcocystosis occur in feral swine that, by contrast, frequently harbor infections with Sarcocystismiescheriana, a related parasite contracted from canids. Parasitology. (*=Equal contributions).

[12] Verma, S.K., Ajzenberg, D., Rivera-Sanchez, A., Su, C., Dubey, J.P., 2015. Genetic characterization of Toxoplasma gondii isolates from Portugal, Austria and Israel reveals higher genetic variability within the type II lineage. Parasitology.

[13] Verma, S.K., Carstensen, M., Calero-Bernal, R., Moore, S.A., Jiang, T., Su, C., Dubey, J.P., 2015. Seroprevalence, isolation, first genetic characterization of Toxoplasma gondii, and possible congenital transmission in wild moose from Minnesota, USA. Parasitol. Res.

[14] Verma, S.K., Su, C., Dubey, J.P., 2015. Toxoplasma gondii isolates from mouflon sheep (Ovis ammon) from Hawaii, USA. J. Eukaryot. Microbiol.

[15] Verma, S.K., Calero-Bernal, R., Lovallo, M.J., Sweeny, A.R., Grigg, M.E., Dubey, J.P., 2015. Detection of Sarcocystis spp. infection in bobcats (Lynx rufus). Vet. Parasitol.

[16] Verma, S.K., Arora, A., Murthy, P. K., 2017. Recombinant Calponin of human filariid Brugia malayi: Secondary structure and immunoprophylactic potential. Vaccine.

[17] Verma, S.K., Joseph, S.K., Verma, R., Kushwaha, V., Parmar, N., Yadav, P.K., Thota, J.R., Kar, S., Murthy, P.K., 2015. Protection against filarial infection by 45-49 kDa molecules of Brugia malayi via IFN-gamma-mediated iNOS induction. Vaccine.

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COMPUTER SKILLS

Software Packages: Adobe Photoshop®, Endnote®, Reference Manager 12®, Graph Pad Prism®, Geneious®, Quantity One®, Microsoft Word, Microsoft Power point, Microsoft Excel. Bioinformatics: Next-Generation Sequencing (NGS), Illumina library preparations, Manage and perform MiSeq run, NGS data analysis, DNA, RNA and Protein sequences alignments, Phylogenetic and metagenomic analysis. Programming Language: Working experience in Linux environment, C, and C++. SCIENTIFIC SKILLS

Editorial board: Journal of Bacteriology and Parasitology. Ad hoc Reviewer for Journal: Zoonoses and Public Health, The Journal of Parasitology, Parasitology Research, Hindawi, and Parasite.

Writing: Drafted 2 thesis (1 each for MS and Ph. D. degree), involved in preparation of 86 manuscripts to publish research work in peer reviewed journals, and 13 posters to present in meetings. Supervising: Worked as an immediate supervisor for 20 undergraduate and 15 graduate students. Collaborating: Collaborated with 10 principal investigators and 5 researchers to accomplish assigned projects. REFERENCES

Dr. Mark C. Jenkins

Microbiologist, USDA-ARS,

10300 Baltimore Avenue, Building 1040, BARC-East, Beltsville, MD 20705-2350, USA Phone: 301-504- 8054, Fax: 301-***-****, Email: ac51g4@r.postjobfree.com Dr. Jitender P. Dubey

Microbiologist, USDA-ARS,

10300 Baltimore Avenue, Building 1001, BARC-East, Beltsville, MD 20705-2350, USA Phone: 301-***-****, Fax: 301-***-****, Email: ac51g4@r.postjobfree.com Dr. David S. Lindsay

Professor, Center for Molecular Medicine and Infectious Diseases Department of Biological Sciences and Pathobiology, Virginia Tech 1410 Prices Fork Road, Blacksburg, VA 24061, USA Phone: 540-***-****, Fax: 540-***-****, Email: ac51g4@r.postjobfree.com I hereby declare that information given here is true and correct to best of my knowledge. Shiv Kumar Verma, Ph.D.

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