Professional Experience Systems
Resume:
Mujib Rahman
Malden, MA 02148
**********@*****.***
Summary:
An organized and devoted biomedical scientist with an educational background of MS in Biochemistry and Pharmacolgy. Strong experience in many aspects of cell and molecular biological technology, with special expertises in cloning, cell culture and generation of stable cell lines, RNAi technology, cell and plate based assay systems (HTS), genome-wide screening of lent-shRNA/CRISPR-gRNA libraries, recombinant protein expression, purification and functional studies. Analytical and critical thinker, pleasant personality, problem-solving and decision-making abilities.
Education:
MS (Biochemistry) 1999
University of Rochester, NY
MS (Pharmacology) 1997
Long Island University, Brooklyn, NY
B. Pharm. (Pharmacy) 1988
University of Dhaka, Bangladesh
Achievements:
●Established a standard protocol for bulk cloning/transfer of phage antibody library into mammalian expression systems, followed by stable transfection and identification of desired antibody genes.
●Successfully identified 5 intermediate regulatory molecules for Integrin activation and function.
●Successfully generated founder mice for inducible expression of MUC1 proto-oncogene.
●Successfully identified 3 dominant-negative mutants for Androgen receptor associated proteins and established their roles in androgen independent prostate cancer progression.
●Research Expertises:
• Expert in many aspects of cell & molecular biology, genome editing, fluorescent microscopy or flowcyto.
• Hands on experience on Cloning, Restriction analysis, Gibson assembly, Infusion HD etc.
• Hands on experience with handling mammalian cell lines, primary, transformed, and stem & progenitor cells
• Hands on Experience with transient or stable cell line generation.
• Hands on Experience with immunohistochemistry, making tissue slides,, embedding, sectioning, staining.
• Hands on Experience with invivo mice generation manipulating stem & progenitor cells.
• Hands on Experience in isolation of monocytes, leukocytes, T cells, Stem & progenitor cells from mice.
• Highly Proficient in RNAi, CRISPR, and use of lentiviral or AAV vectors.
• Highly Proficient in lenti/retro-viral production, titering (plaque assay, flowcytometry), Infection, cell based
assays.
• Highly Proficient in library screening using lenti-shRNA/CRISPRgRNA.
• Highly Proficient in protein and nucleic acid isolation/analysis (SDS page,Western blot, Commasie, silver
staining, ELISA, PCR, qPCR etc.)
• Highly Proficient in gel based analysis (Norther, Southern blots) • Highly Proficient in cell based and plate based assay systems for target identification and or validation. • Highly Proficient in High throughput screening (HTS), High content imaging (cellworks and ImagExpress),
ELISA and Flowcyto analysis.
• Very proficient in leukocyte isolation (T cell and B cell) and genetic
modifification (CRISPR editing) .
• Very familiarity and knowledge in adoptive T cell, CAR-T, or TCR therapeutics.
• Very familiar with AKTA, TECAN, OCTET.
• Highly proficient in verbal and written communication skills
• Can perform independently.
• Excellent computer skills including extensive experience with Microsoft Word, Excel, Power Point, Biobook,
Graphpad, Laser gene, NTI vector.
Professional Experience
Pfizer Inc. (GBT) Cambridge, MA 5/10/16 - 5/5/17
Bench Scientist
●Established Phage to CHO expression systems: a standard protocol for cloning of phage antibody library into mammalian expression systems. Bulk cloning/transfer of phage antibody genes (library) into CHO vector generating pooled mammalian expression library. Developed stable cell line in CHO suspension cells; seeded and isolated single cell (96 and 384 well plates); screened for secreted antibody; recovered single cell/gene expressing desired antibody.
Instruments used:
●Centrifuges, Biomek washers, Automated Liquid handling machines, Cloning, Electroporator (Bacterial), Aseptic cell culture (Stable), Neon-electroporation, 96-, 384 well plates, Shake Flasks, ELISA, FACS (Fortesa, Bio Rad S3, Canto),
Massachusetts General Hospital, Boston, MA 5/2007 - 5/2016
Asst. Biologist
●Successfully identified 5 intermediate regulatory molecules for integrin activation and function (adhesion/ligand binding) by a high throughput screening of shRNA library targeting human Phosphatases.
●Completed a genome wide screening by pooled CRISPR-gRNA library targeting all human genes (about 19,000 genes, 84K constructs) to screen out global integrin regulators. Further identification and validations are being going on in the lab.
●Isolated Monocytes, Leukocytes (B and T cells), Platelets and used for cell based assay systems. I did culture T cells and B cells, infected with Leti-shRNA-GFP (DUSP22), and Lenti-CRISPR-gRNA, isorted GFP cells and used for cell based assays for checking Integrin expression and activation. Also, I used Many cell lines expressing T cells.
●Utilized immunohisto-technology, making tissue slides,, embedding, sectioning, staining.
●Utilized High throughput screening (HTS), High content imaging (cellworks and ImagExpress),
ELISA and Flowcyto analysis.
●Generated in-vivo transgenic shRNA mice by manipulation of putative stem and progenitor cells from donor mice. Studied in-vivo integrin activation.
Instrumentation used:
SEC, FPLC, AEX, SDS-PAGE, Commassie, Silver staining, IP, Co-IP, Pull-down, northern blot,
western blot, Mammalian cell culture (96-, 384-well plates and shake flasks), cell transfection
(transient/stable), centrifugation, PCR, qRT-PCR, Electroporation, FACS, ELISA, High content
Imaging, Cellworks, ImageExpress Micro, Spectromax. Flow Cytomer LSR II, FACS Calibur, Fortesa,
FACSARIA DIVA, FlowJo.
Dana Farber Cancer Institute, Boston, MA 2003-2007
Research Scientist
●Successfully generated founder mice for inducible expression of MUC1 proto-oncogene.
●Using in vitro cell-based assay systems and in vivo transgenic and inducible mouse models attempted to elucidate role of MUC1 in tumorigenicity and chemo-resistance in prostate, breast, and ovarian cancers. expressing T cells.
●Routinely utilized immunohisto-technology, making tissue slides,, embedding, sectioning, staining.
●Isolated melanocytes from 3 day old pups.
●Isolated stem cells from endocrine glands (mTert-GFP expressing mice).
University of Rochester, Rochester, NY 1999-2003
Research Tec
●Successfully identified 3 dominant-negative mutants for ARA54, ARA70 and ARA55 using yeast-two hybrid screening.
●Subsequently used these dominant-negative mutants as tools to studying the functional roles of androgen receptor (AR) coregulators ARA55 and ARA70 in prostate cancer progression.
●Validated these results using RNA interference.
Member in Honor Societies
●American Association for Advancement of Science (AAAS)
●American Society for Biochemistry and Molecular Biology (ASBMB)
●Pharmacy Honor Society, USA
●Society of Toxicology, USA
●Bangladeshi American Pharmacist Association (BAPA), USA
●Pharmacy Graduates Association (PGA), Bangladesh
●Bangladesh Pharmaceutical Society (BPS), Bangladesh
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