**** ****** **** *****, #**, Bronx NY-10461
firstname.lastname@example.org – 347-***-****(M)
OBJECTIVE: Obtaining a Scientist position.
Ph.D. Biochemistry, University of Calcutta, Calcutta, West Bengal, India. 2001.
Thesis title: “Studies on the ribosomal RNA of intestinal parasite Giardia lamblia”
M.Sc. Biochemistry, University of Calcutta, Calcutta, West Bengal, India. 1993.
B.Sc. Chemistry, University of Calcutta, Calcutta, West Bengal, India. 1991.
AWARDS AND FELLOWSHIP:
CSIR/UGC Fellowship, India. 1995- 2000.
ADDITIONAL INFORMATION: Permanent resident (green card holder) of United States.
Albert Einstein College of Medicine, New York, USA (Dept. of Cell Biology).
Associate (July 2011-Present)
Initiated and overseen project to identify new substrates of CDK6 in murine erythroleukemia cell lysates. This required managing the collaborations with Dr. K. Shokat (HHMI Prof. at UCSF School of Medicine) and Dr. R. Fisher (Prof. at Mount Sinai School of Medicine).
Generated transgenic mouse over-expressing CDK6 in the erythroid lineage. Maintained the breeding colonies and did the genotyping.
Responsible for overseeing the many additional mutant mouse colonies for the lab including breeding, genotyping and determining number of mice for each colony.
Over-expressed kinase active, inactive and analogue sensitive forms of CDK6 (AS-CDK6) in both MEL (murine erythroleukemia) and erythroid progenitor cells and demonstrated that CDK6 is an oncogene in red blood cells and kinase activity of CDK6 is required for blocking differentiation.
Co-expressed and purified active CDK6/Cyclin D3 and AS-CDK6/Cyclin D3 complex using baculo virus system and identified new and novel substrates of CDK6 by chemical genetics.
Collaborated with several postdoctoral researchers to ensure successful and timely completion of projects.
Research Associate (August 2005 – June 2011)
Initiated and oversaw projects to understand how CDK6 (a cell cycle regulator) and PU.1 (an important hematopoietic transcription factor) co-ordinates to block differentiation of murine erythroleukemia cells. This required managing the collaborations with Dr. M. Socolovsky (Assoc. Prof. at UMass. Medical School) and Dr. M. Brand (Senior Scientist, Ottawa Hospital Research Institute, Canada)
Demonstrated that CDK6 phosphorylates and stabilizes PU.1.
Identified several new interactors of transcription factor PU.1 by co-immuno-precipitation and mass spectrometry and confirmed the interactions in-vitro.
Demonstrated PU.1-dependent DNA methylation change in fetal liver erythroid progenitor cells.
Initiated projects to understand how PU.1 gene expression is regulated in erythroid cells.
University of California, San Diego, USA (Dept. of Chemistry and Biochemistry).
Postdoctoral Research Fellow (August 2000 - July 2005)
Initiated projects to identify functional groups within 16S rRNA important for ribosome assembly and EF-G dependent tRNA translocation.
Identified non-bridging phosphate oxygens within 16S rRNA that are important for 30S ribosome assembly, association with the 50S ribosomal subunit and tRNA translocation using phosphorothioate modification -interference analysis.
Identified bases in 16S rRNA important for tRNA translocation using a novel modification-interference assay.
Advised two junior postdoctoral researchers and supervised two undergraduate researchers.
Published research in peer-reviewed scholarly journal article.
Presented research at symposiums with more than four hundred attendees.
University of Calcutta, Calcutta, West Bengal, India (Dept. of Biochemistry).
Graduate student (March 1995-july 2000)
Utilized the intergenic spacer region of 16S rRNA gene of protozoan parasite Giardia lamblia to
develop a PCR-based diagnostic tool for giardiasis.
Developed an in vitro assay system for studying Giardia rRNA processing.
Expressed and purified a nuclear protein fibrillarin of Giardia lamblia in E.Coli.
Managed and mentored several undergraduate researchers.
Published research in several peer-reviewed scholarly journal articles.
Served as teaching assistant in Biochemistry lab courses.
TECHNICAL SKILLS: Mammalian cell cultures (fetal liver and bone marrow erythroid progenitor cells and other transformed cell lines like 293T, MEL, 32D, CMK, SKM and NB4 ); FACS sorting and analysis; transient and stable transfection in mammalian cells; retroviral and lenti viral transduction in erythroid progenitor cells; siRNA, mir like shRNA and CRISPR/Cas9 mediated gene knockdown in mammalian cells; colony forming assays and bioassays for proliferation, differentiation and kinases; protein expression and purification from insect cells and bacteria; chromatin immunoprecipiation (CHIP) assay; generation of transgenic mouse; DNA methylation studies by mass array and ERRBS; RNA structure-function studies by modification-interference analysis and primer extension; general molecular biology techniques such as sub-cloning, PCR, RT-PCR, QPCR, site directed mutagenesis, co-immunoprecipitation, westerns, northerns, southerns.
COMPUTER SKILLS: Microsoft Office (Word, PowerPoint, Excel), Adobe.
1.S.Ghosh, K. Choe, R. Pop, M. Socolovsky and A. Skoultchi. CDK6 blocks differentiation of murine erythroleukemia cells by phosphorylating and stabilizing PU.1. (Manuscript close to submission).
2.S.Ghosh, R.Levin, S. Larochelle, R. Fisher, K. Shokat and A. Skoultchi. Identification of CDK6 substrates in murine erythroleukemia cell lysates (Manuscript close to submission).
3.F. Yue et al. (2014). A comparative encyclopedia of DNA elements in the mouse genome. Nature, Nov 20; 515(7527):355-64.
4.X. Shi, K.Chiu, S. Ghosh and S. Joseph. (2009). Bases in 16S rRNA Important for Subunit Association, tRNA binding, and Translocation. Biochemistry, July 28; 48(29): 6772-82.
5.S. Ghosh and S. Joseph. (2005). Non-bridging phosphate oxygen in 16S rRNA important for 30S subunit assembly and association with the 50S ribosomal subunit. RNA, May; 11(5) 657-67.
6.S.Ganguly, S. Ghosh, D. J. Chattopadhyay and P. Das. (2004). Antisense molecular beacon strategy for in situ visualization of snRNA and fibrillarin protein interaction in Giardia lamblia. RNA Biology, May; 1(1): 48-53.
7.S.Ganguly, S. Ghosh, D. J. Chattopadhyay and P. Das. Cover Page Photograph. (2004). Colocalization of fibrillarin protein inside G. lamblia nuclei. RNA Biology. (1), May.
8.S. Ghosh, R. Ghosh, P. Das and D. J. Chattopadhyay. (2001). Expression and purification of recombinant Giardia fibrillarin and its interaction with small nuclear RNAs. Prot. Exp. And Purification 21, 40-48.
9.S. Ghosh, A. Debnath, A.Sil, S. De, D. J. Chattopadhyay and P. Das. (2000). PCR detection of Giardia lamblia in stool: targeting intergenic spacer region of multicopy rRNA gene. Mol. And Cell. Probes. Jun; 14(3) 181-189.
10.A. K. Sil, P. Das, S. Bhattacharya, S. Ghosh and D. J. Chattopadhyay. (1998) Cloning of ribosomal RNA genes from an Indian isolate of Giardia lamblia and the use of intergenic nontranscribing spacer regions in the differentiation of Giardia from other enteric pathogens. Journal of Biosciences, 23(5), 557.