Daoyuan Lu, Ph.D.
Address: **** ******* ****, **********, **, 19810
Passionate, highly knowledgeable and result-driven researcher with hands-on experience in cell biology, molecular biology, pharmacogenetics and biochemical analytical laboratory techniques.
Excellent team player and strong builder of prolific collaborations in different aspects of basic and applied research leading to long-lasting research projects and >14 peer-reviewed scientific publications.
Creative and self-motivated individual with easy-integration in a multicultural environment with unique combination of detail-oriented mindset, driven personality, analytical skills and proven ability to beat tight deadlines by working in a fast-paced work environment leading to discovery of novel targets and diagnostics in cancer and neurodegenerative diseases.
08/2017- Present Postdoctoral Researcher, Department of Oncology and Diagnostic Sciences, Greenebaum Cancer Center, University of Maryland, Baltimore, Maryland (Under H1b visa and recently received employment authorization card)
Project: Investigate the molecular mechanisms of interaction between angiopoietin like 4 and its receptor neuropilin-1/2 in angiogenesis in ischemic retinopathy and tumor metastasis
Conduct mammalian cell culture (Human endothelial cell, cancer cell lines and CHO cells), stable cell line development, viral transduction of mammalian cells, Adenovirus, Lentivirus and Adeno-associated viral (AAV) vector construction, fluorescent microscopy, cell counting, confocal imaging and Flow cytometry
Perform ELISA, Co-IP, western blot, 2-D DIGE, immunohistochemistry/ fluorescence and other immunoassays and cell based assays
SDS PAGE gel electrophoresis, molecular cloning, qPCR/qRT-PCR, Enzyme assays
10/2015- 07/2017 R&D Director, Genowise Clinical Laboratory Co. Ltd,China; Institute of Precision Medicine and Diagnostics, Central South University; State Key laboratory of Molecular Oncology, translation center in Hunan; Joint lab of Center for genomics, University of California, San Diego
Lead the group to develop Human CA125 and HE4 ELISA detection kit by utilizing the cancer biomarkers for early detection of ovary cancer and “Pharmacogenomics Kit for One Hundred Medicines” (developing the GenescanTM genotying system for genotying the genes involved in pharmacokinetics) in conjunction with American Diagnosis, Inc (San Diego, CA).
Conducted mammalian cell culture and human cancer cell lines, viral transduction, Lentivirus and Adeno-associated viral (AAV) vector construction and purification, microscopy, confocal imaging, and cell based assay, perform quantitative PCR and DNA sequencing on tumor patient samples within cGMP, GLP environment.
Wrote and implemented SOPs in a compliant laboratory. Performed method transfer, development and validation.
12/2014 – 07/2015 Visiting Scientist, Department of Molecular and Medical Genetics, Oregon Health and Science University, Portland, Oregon
Designed and executed the project: The role and molecular mechanisms of Ubiquitin specific protease USP36 in DNA damage response in human cancer cells.
10/2010 – 10/2014 Postdoctoral Research fellow, Laboratory of Neuroscience, National Institute on Aging, National Institutes of Health, Baltimore, Maryland
Projects: Investigated the novel role and molecular mechanisms of GIT2 (G – protein Coupled Receptor Interacting protein 2) in promoting the repair of DNA damage, particularly DNA double strand breaks in human neuroblastoma cells and aging mice brain.
Studied the molecular mechanisms that regulate mitochondria biogenesis and how alterations in these mechanisms contribute to the pathogenesis of neuro developmental and neurodegenerative diseases
Investigated the role and molecular mechanisms of reactive oxidative species (ROS) in neural development and neural plasticity in primary neurons from embryonic SOD2 deficient mice and using SOD2 knockout mice as a model
Studied the role and molecular mechanisms of the mitochondrial deacetylase Sirt3 in maintaining integrity and function of mitochondria and promoting neuronal survival in response to stresses in primary neurons from embryonic Sirt3 deficient mice and using Sirt3 gene modified mice as a model
Explored the novel roles of peroxisome proliferator-activated receptor γ co-activator 1α (PGC1α), a master regulator of mitochondrial biogenesis, in regulating neural development and neural plasticity
Using state-of-the-art technologies including two-photon confocal microscopic imaging, in vivo stereological injection of adeno associated virus (rAAV) to over-express or knockdown (shRNA) specific genes involved in mitochondrial biogenesis or ROS regulators to advance an understanding of mitochondria in neurogenesis, adult neuroplasticity and disease states
These studies established new dimensions of mitochondrial functions in the neurogenesis and neurodegenerative diseases.
09/2005 – 07/2010 Ph.D. Cell Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China
09/2002 – 07/2005 M.S. Molecular Genetics, Wuhan University, Wuhan, China
Cell culture experience (cell lines and primary culture of neurons and cardiomyoctes), viral transduction of mammalian cells, microscopy, cell counting, stable cell line development, basic bioreactor operation, confocal imaging and Flow cytometry
DNA, RNA extraction and purification, molecular cloning (plasmid DNA expression vector construction, protein expression and purification (FPLC)
ELISA, western blot, immunohistochemistry/fluorescence immunoprecipitation, GST pull down, CHIP assay and other immunoassays and cell based assays
DNA and protein gel electrophoresis, PCR/qPCR/ddPCR, Enzyme assays
Lentiviral, Adenovirus and Adeno-associated viral (AAV) vector construction and purification
RNAi and CRISPR/CAS9 techniques
Experienced with animal experiments (mice, rat, zebrafish) and other basic biological experiments (blood test, dissection and specimen making of plants, insects and small animals)
cGMP working experience and training to support CMC in drug product testing and development
pH meter, titration, sample preparation and other Chemistry/Biochemistry/Analytical wet Chemistry Laboratory techniques, GLP
Purification of small molecules and biomolecules with HPLC and FPLC, including Ni-affinity, ion-exchange (IEX) chromatography and Characterization of biological samples with fluorescence plate reader
Quantification of biomolecules with LC-MS/MS
Familiar with Microsoft Office suite (Word,Excel, Powerpoint), data entry
Origin, Image J, Swiss-PDB Viewer, LSM Image Examiner and Endnote
Bioinformatics, biostatistics and large data analysis
1.Lu daoyuan,Song Ping, Chen YunGui, Peng MaoYu, Gui JianFang. Expression of gene vasa during gonadal natural sex reversal of ricefield eel Monopterus Albus. Acta zoological Sinica. 51(3): 469-475, 2005.
2. SaiJun Mo, Ping Song, DaoYuan Lu, YunGui Chen, Wei Zhou, WuMing Gong, ZuoYan Zhu. Zebrafish z-otu, a novel Otu and Tudor domain-containing gene, is expressed in early stages of oogenesis and embryogenesis. Biochimica et Biophysica Acta. 1732 (2005) 1–7.
4. DaoYuan Lu, Ping Song, YunGui Chen, WuMing Gong, SaiJun Mo. Cloning and characterization of full length of a novel zebrafish gene Zsrg abundantly expressed in the germline stem cells. Biochemical and Biophysical Research Communications. 329 (2005) 632–637.
5.Ding Ye, DaoYuan Lu, Ping Song, MaoYu Peng, YunGui Chen, Ming Guo, QiWen Yang, and YinChang Hu. Cloning and Characterization of a Rice Field Eel vasa-Like Gene cDNA and Its Expression in Gonads During Natural Sex Trans- formation. Biochemical Genetics, Vol. 45, Nos. 3/4, 211-224, April 2007.
6.YuZhen Li*,DaoYuan Lu*(co-first author), WeiQi Tan, JianXun Wang, and PeiFeng Li. p53 Initiates Apoptosis by Transcriptionally Targeting the Anti-apoptotic Protein ARC. Molecular and Celluar Biology, Jan. 2008, Vol. 28, No. 2, p. 564–574.
7.WeiQi Tan*, Kun Wang*, DaoYuan Lu, and PeiFeng Li. Foxo3a inhibits cardiomyocyte hypertrophy through transactivating catalase. Journal of Biological Chemistry, 2008 Oct 31; 283(44):29730-9.
8. Siddiqui Sana, Fang Meng, Ni Bin, Lu Daoyuan, Martin B, Maudsley S. Central role of the EGF receptor in neurometabolic aging. Int J Endocrinol. 2012;2012:739428.
9.DaoYuan Lu*, JinPing Liu*, JianQin Jiao and PeiFeng Li. Foxo3a prevents apoptosisby
regulating calcium through the apoptosis repressor with caspase recruitment domain. Journal of Biological Chemistry. 2013 Mar 22; 288(12):8491-504.
10. Daoyuan Lu, Huan Cai, Sung-Soo Park, Bin Ni, Sana Siddiqui, Meng Fang, Richard T. Premont, Michael Seidman, Ionoa Bodogai, Arya Biragyn, Caitlin M. Daimon, Bronwen Martin, Stuart Maudsley. Nuclear GIT2 is an ATM substrate and promotes DNA repair. Molecular and Cellular Biology. 2015 Apr 1;35(7):1081-96. doi: 10.1128/MCB.01432-14.
13. Cheng A, Yang Y, Zhou Y, Maharana C, Lu Daoyuan, Peng W, Liu Y, Wan R, Marosi K, Misiak M, Bohr VA, Mattson MP. Mitochondrial SIRT3 Mediates Adaptive Responses of Neurons to Exercise and Metabolic and Excitatory Challenges. Cell Metabolism. 2016 Jan 12;23(1):128-42.
14. J.Janssens, Daoyuan Lu, Bin Ni, W.Chadwick, S.Siddiqui, A.Azmi, H.Etienne, A.Jushaj, J.van Gastel, B.Martin, S.Maudsley. Development of Precision Small Molecule Proneurotrophic Therapies for Neurodegenerative Diseases. Vitamins and Hormones. 2017;104:263-311.
15. Daoyuan Lu, Ying Yang, Ruiqian Wan, Mark P. Mattson and Aiwu Cheng. Manganese Superoxide Dismutase deficiency suppressed the cortical neural survival, neurite development and plasticity. In submission.
16. Akrit Sodhi, Tao Ma, Monika Deshpande, Kathy Jee, Deepak Menon, David Poon, Daoyuan Lu, and
Silvia Montaner. Angiopoietin-like 4 binds Neuropilins and cooperates with VEGF to induce
diabetic macular edema. In submission.