Manager Project

FRANCES-CAMILLE S. CHENG, Ph.D.

**** **** ****** *** **, Elmhurst, NY 11373 M: 646-***-**** *************@*****.***

SUMMARY

Experienced Biomedical Scientist with extensive experience in protein and nucleic acid research. Demonstrated history of effective scientific communication. Proven ability to initiate interdisciplinary collaboration. Skilled in NMR Spectroscopy, Materials Science, Protein Chemistry, Spectroscopy, and Biophysics. Strong education professional with a Doctor of Philosophy (Ph.D.) focused in Biochemistry, Biophysics from Albert Einstein College of Medicine.

HIGHLIGHTS

●10+ years of experience in structural biology

●Enthusiastic communicator of scientific content to a variety of audiences

oWritten and edited several scientific papers, abstracts and posters

●Excellent capacity for teamwork

●Ability to successfully meet scientific challenges and execute diverse scientific projects both individually and within teams

●Diligent to deadlines of multiple tasks to ensure completion of project goals

●Experience with laboratory budgets and personnel management

●Outstanding manager and mentor of trainees

●Experience with Microsoft programs and Endnote

●Relevant therapeutic areas:

oOncology

oNeurology

oImmunology

oNutrition

oInfections and infectious disease

EXPERIENCE

7/2017 – Present Scientific Writer

Target Health, Inc., NY, NY

●Write IND and NDA documents for submission to regulatory authorities, including: Protocols, CRFs, model informed consents, final integrated clinical study reports, and integrated summaries of safety (ISS) and efficacy (ISE), as requested.

●Write documents associated with clinical trials which are not necessarily part of regulatory submissions, including monitoring guidelines, and manuscripts.

●Coordinate and conduct team review and sign-off of document deliverables, including distribution drafts, chairing review, incorporating revisions and completion of the sign-off procedure.

●Adhere to FDA or other appropriate regulatory body guidelines with respect to document content and style.

●Meet Company’s expectations by following any templates, style guides, or SOPs provided by the Company for document development.

3/2015 – 6/2017 Postdoctoral Research Fellow

Advisor: Sebastian Klinge, PhD

Protein and Nucleic Acid Chemistry, The Rockefeller University, NY, NY

●Data collection, processing and analysis

●Assist faculty researchers and other collaborators in planning and designing research experiments; establishing priorities and recommending schedules, time tables and costs

●Presentation of project results to internal and external parties

●Operate specialized scientific equipment

●Produce written reports on the result of the analyses

●Effectively use available technology to facilitate the success of the project

●Engage in writing of technical publications and reports

●Supervise and train graduate students and rotation students

●Project 1: Structural studies of Dbp8, an RNA helicase implicated in ribosome biogenesis

●Optimized the expression and purification of recombinant Dbp8 (DEAD Box Protein 8)

●Identified native RNA substrate using novel UV cross-linking and analysis of cDNA (CRAC) method

●Performed crystallographic screens of protein and protein-RNA complex for structure determination studies using X-ray crystallography

●Project 2: Elucidate the functional architecture of RNA Polymerase III using cryo-electron microscopy

●Utilized multiyeast expression platform for the production of a eukaryotic multiprotein complex

●Trained in FEI TECNAI G2 Spirit BioTwin Transmission Electron Microscope and FEI Titan Krios cryo-electron microscope

12/2013 – 2/2017 F1000 Associate Faculty Member

●Write brief, accessible reviews to summarize value of scientific articles

●Write recommendations about current scientific literature in the fields of:

oBiochemistry

oBiotechnology

oStructural biology

oMolecular biology

oChemical biology

oCancer biology

8/2009 – 12/2014 PhD Candidate

Advisors: Mark Girvin, PhD, Michael Brenowitz, PhD, Matthew Levy, PhD

Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY

Investigating the atomic-resolution structure of high affinity protein-specific RNA aptamers

●Isolated sub-nanomolar affinity RNA aptamers against HIV-1 Matrix protein using SELEX (Systematic Evolution of Ligands by Exponential enrichment)

●Identified minimal, compact aptamer structure using chemical and enzymatic footprinting assays

●Utilized Nuclear Magnetic Resonance (NMR) spectroscopy to characterize aptamer binding site on the protein

●Determined the 2.0 Å crystal structure and functional characteristics of a lysozyme-specific RNA aptamer

●Engineered and biophysically characterized an RNA aptamer against myristoylated HIV-1 Matrix protein

5/2008 – 8/2008 Summer Research Student

Advisor: Georges Mer, PhD

Department of Biochemistry, Mayo Clinic, Rochester, MN

Structural studies of TopBP1

●Acquired and analyzed NMR three-dimensional triple resonance experiments of Topoisomerase (DNA) II Binding Protein 1 (TopBP1)

06/2005-08/2009 Undergraduate Research Student

Advisor: Steve Greenbaum, PhD

Hunter College at the City University of New York, NY, NY

Investigate the structural characteristics and self-assembly process of silk-elastin copolymers (SELPs) using solid-state NMR spectroscopy

●Experience in one-dimensional solid state-NMR spectroscopy methods:

Cross-polarization/Magic angle spinning (CP/MAS), Total suppression of spinning sidebands (TOSS)

Investigate the dynamics of ion transport in disordered media to develop materials for fuel cells

●Use of solid state-NMR spectroscopy and electron paramagnetic resonance (EPR) to study local atomic arrangements and electronic band structure of noble metal alloys for fuel cell catalysts

EDUCATION

2014 Ph.D. Biomedical Science, Biochemistry and Biophysics concentration

Albert Einstein College of Medicine, NY

2011 M.Sc. Biomedical Science

Albert Einstein College of Medicine, NY

2009 B.A. Physics, cum laude

B.A. Chemistry, cum laude

Minor: Mathematics

Hunter College at the City University of New York

MARC (Minority Access to Research Careers) Scholar, 2007-2009

Hunter College Physics Club President, 2007-2009

SKILLS

Leadership skills

●Promoted lab social interactions (planned annual lab hiking trips, holiday dinners, lab retreats)

●Volunteered in science outreach programs with the Einstein Minority Student Association (EMSA)

●Represent postdoctoral associates/fellows for the Rockefeller University Diversity Initiative (RUDI)

●Mentored and supervised several trainees:

o3 undergraduate students – All three entered PhD programs

o2 high school summer rotation students – Both enrolled in college (one is pre-medical, and the other is pre-graduate school)

o1 visiting scholar – Mentored scientist in basic NMR methods

o1 lab technician – Trained in general laboratory techniques

Teaching Skills

●Bi-annual guest lecturer at Hunter College SCI 200 course with the purpose of introducing undergraduates to scientific research and the scientific method

●Teaching assistant for graduate-level biochemistry class for two years

oPrepared PowerPoint review materials and problem sets

●Participated in outreach activities to promote scientific research to underserved schools in the Bronx and Queens areas.

Managerial skills

●Managed one lab technician and several trainees

●Assisted with the development and implementation of a tiered mentoring platform for the Women in Science at Rockefeller (WISeR) group

Technical skills Biophysical methods

Solution-state NMR spectroscopy, X-ray crystallography, circular dichroism,

dynamic light scattering, isothermal titration calorimetry, analytical

ultracentrifugation, chromatography (IMAC, IEX, SEC)

Molecular biology

DNA cloning, primer design, PCR, isolation of genomic DNA, RNA isolation,

bacterial and yeast transformation, gel electrophoresis, directed evolution of

aptamers

Protein and nucleic acid biochemistry

Protein expression and purification of recombinant proteins in bacteria and

yeast, selective labeling of proteins for NMR, in vitro RNA transcription, solid-phase oligonucleotide synthesis

Computer skills

Structure determination: Semi-automated footprinting analysis (SAFA)

software, PyMol, Chimera, CcpNMR analysis suite, SEDNTERP, DCDT+, Mfold, Kinefold, UNAFold, SARNA-Predict, RNAstructure

General software: Microsoft Word, Microsoft Excel, Microsoft Powerpoint,

Inkscape, Adobe Photoshop, GraphPad Prism, Origin, Endnote

PUBLICATIONS Previously published under maiden name, Padlan

1.Khrapunov S, Tao Y, Cheng H, Padlan CS, Harris R, Galanopoulou AS, Greally JM, Girvin ME, Brenowitz M. ‘MeCP2 Binding Cooperativity Inhibits DNA Modification-Specific Recognition.’ Biochemistry. Vol 55 (31) pp 4275-4285. 2016.

2.Padlan CS, Malashkevich V, Almo SC, Levy M, Brenowitz M, Girvin ME. An RNA aptamer possessing a novel monovalent cation-mediated fold inhibits lysozyme catalysis by inhibiting the binding of long natural substrates. RNA. Vol 20 pp 1-15. 2014.

3.Padlan FS, Bachu R, Rouhanifard S, Brenowitz M, Schlatterer JC. ‘Monitoring Equilibrium Changes in RNA Structure by 'Peroxidative' and 'Oxidative' Hydroxyl Radical Footprinting’. J. Vis. Exp. (56), e3244, doi:10.3791/3244 (2011).

FELLOWSHIPS

2016 – 2017 Women and Science Postdoctoral Fellowship

INVITED TALKS

1.A novel monovalent cation stabilized binding platform mediates an aptamer – protein complex. 23rd Congress - International Union of Crystallography. 2014

2.Old proteins & new tricks: A novel cation-mediated fold mediates a lysozyme-aptamer complex. New York Structural Biology Discussion Group, New York Academy of Science. 2014

3.Structure Determination of RNA aptamer-protein complexes. Gibbs Conference on Biothermodynamics. 2012

POSTERS

●New York Structural Biology Discussion Group, 2013

●Biophysical Society Annual Meeting, 2013

●New York Structural Biology Discussion Group, 2012

●New York Structural Biology Discussion Group, 2011

●Gibbs Conference on Biothermodynamics, 2011

●Gordon Conference – Computational aspects of Biomolecular NMR, 2011

●Annual Biomedical Research Conference for Minority Students, 2008

o2nd place award in Engineering, Physics & Mathematics

●National Society of Black and Hispanic Physicists Conference, 2008

●National Society of Black and Hispanic Physicists Conference, 2007

SOCIETIES

2011 – Present New York Academy of Sciences

2010 – Present Biophysical Society

2009 – Present American Association for the Advancement of Science (AAAS)

REFERENCES

●Mark Girvin, Ph.D. ****.******@********.**.*** 718-***-****

●Michael Brenowitz, Ph.D. *******.*********@********.**.*** 718-***-****

●Matthew Levy, Ph.D. *******.****@********.**.*** 718-***-****

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