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Research University

Hyderabad, Telangana, India
3.5-4 lakhs per annum
March 10, 2018

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Mobile no: 871-***-****


To make the perception of mounting unique peaks in my career achievement with the skills, knowledge and perfection to the best of my achievable proficiency for mounting the higher goals of the esteemed organization with the ambitious status and also to gain huge exposure for the upgrading methodologies for a being innovative and flexible. EDUCATIONAL QUALIFICATIONS:

Pursuing PhD from Osmania University and working under the Guidance of Dr.M.P.Pratap Rudra and got 60% in the course work and thesis to be submitted.

M Sc. Biochemistry with 72% from Chaitanya PG College, Kakatiya University in 2009.

B Sc. (Bi.Mi.C) with 75% from Holy Cross Degree College Women, BHEL, R.C.Puram, Hyderabad in 2007.

Intermediate (B.P.C) with 71% from Board of intermediate in Kakatiya Junior College, SR Nagar, Hyderabad in 2004.

SSC with 80% from St. Arnold’s High school, BHEL, R.C.Puram, Hyderabad in 2002. Key Skills:

Strong knowledge in Microbiology, Molecular Biology and biochemical analysis protocols

Excellent in equipment handling

Expertise in performing tests and also grasps the new upcoming methodologies and applying them in a specific context

Reveals the sources of difficulties in a situation and recommended solutions for perfect handling of man and machine power

Literature Survey

Paper and poster presentation

Paper publications to be prepared, edited and formatted by my own skills.

Conducted Conferences

Good in measurements and readings

Computer literacy with knowledge of MS office (Power point and documentation proficiently) & internet browsing.


Good analytical and communication skills

Creative presentation and logical thinking

Hardworking nature, self-confident, sincere and enjoys the team work

Flexibility to work hard for long hours and patient enough until the goal I accomplish. Instruments Handled:

Paper Chromatography, Thin Layer Chromatography, Column Chromatography and Gas Chromatography and UV Spectrophotometer, Centrifugation, Colorimeter, Vacuums evaporators or Rota vapors, Hemocytometer.

Industrial Skills: Identifies the good qualitative and quantitative outputs for resolving the skillful methodologies, energetic work ethics for approaching the higher preferences in the present research and development.

General Skills: ELISA, RT-PCR, Primer Designing, Agarose Gel Electrophoresis, Purification by Affinity Chromatography.

Cell and Molecular Biology: Plasmid Isolation, Restriction Digestion, Ligation, Competent cell preparation, Transformation in E. Coli, Clone Selection, Genomic DNA extraction, Cell Viability Assay.

Cell Culture Skills: Preparation of Medium, Maintenance of Cell lines (Splitting the cells), Cell Count, Anti-Cancer Activity (MTT Assay), Annexin –V Assay, Flow Cytometry. Biochemical and Immunological Skills: Paper Chromatography, Thin Layer Chromatography, Column Chromatography and Gas Chromatography and UV Spectrophotometer, Centrifugation, Colorimeter, Vacuums evaporators or Rota vapors, Spectrophotometer, Colorimetry, Hemocytometer.


Dissertation with “Synthesis of biologically active prochiral alcohols by using biocatalysts” from IICT (Indian Institute of Chemical Technology)HYDERABAD of which it is dealing with the research for upgrading the Drug and Cosmetic industries and also upgraded my technical knowledge in biochemical techniques.


Participated in “National seminars on recent trends in Microbiology” organised by Department of Microbiology, Chaitanya PG college, Kakatiya University on 5-6th October 2007.

Attended workshop on “Molecular techniques” organized by Department of Microbiology, Chaitanya PG College, Kakatiya University.

Participated in one day workshop in “Ethical issues of Animal House and Animal experimentation” held on 4-09-2012, at Department of Zoology,University college of Science,Osmania University,Hyderabad.

Participated and given paper presentation entitled “Stigmaphyllone littorale leaves mediated silver nanoparticles biosynthesis and characterization” in the International Conference, Global Meet of Biologists-2012, at Indian Institute of Chemical Technology from 26th-28th December-2012.

Participated in National conference “Declining Interest in Science Education and Research Among Students:Reasons and Remedies” at IICT on 29th-30th March 2013.

Attented fieldworks in Yashoda hospitals Secunderabad, Government mental hospital Erragadda, Unani hospitals Erragadda.

Participated and given paper presentation entitled “Influence of distinctive sources on Hydrogen Production from Photosynthetic bacteria isolated from marine water of Goa”, in Indo-US Conference on Advanced Lignocellulosic Biofuels (Indo-US CALB-2014).

Presented the Oral presentation in “International Conference on Biochemistry, Nutrition & Pharmacy in Human Welfare: Recent Trends and Future Challenges” as titled

“BIOFUEL PRODUCTION FROM RHODOPSEUDOMONAS FAECALIS MPPR 001 PHOTOSYNTHETIC BACTERIA USING GAS CHROMATOGRAPHY” On September 3rd - 5th, 2015 at Department of Biochemistry, Osmania University. Research Experience:

SPECIALISATION: Microbial Biochemistry.

THESIS TITLE: “Biofuel Production from Marine Phototrophic bacteria” Supervisor: Dr.M.P.Pratap Rudra, Department of Biochemistry, Osmania University. Brief Outlines of Research Work:

Collection of different marine water samples from Kanyakumari (Indian Ocean), Vizag

(Bay of Bengal), and Goa (Arabian Sea).

Isolation of organism (Purple non-sulphur bacteria or an oxygenic Phototrophic bacterium) from different marine samples.

Characterization of organism by 16s rRNA sequencing for identification of strain.

Deposition of identified organism to NCBI for specific identified strain for my isolated cultures.

Optimization studies of Different carbon, nitrogen, growth factors and PH variation were checked by identified an oxygenic phototrophic bacteria and their growth variation in different parameters.

Biochemical Characterizations like Carbohydrate, Protein, Cellular carbon & nitrogen ratio, bacterial pigments, total amino acids and also indole acetic acid estimations were done for identified isolated organisms from different marine water samples.

For all the above parameters which were checked for Hydrogen gas production by water displacement method and also by Gas Chromatography.

The isolated strains were expressed for cloning, gene expression and characterization of aminolevulinic acid synthase and also the shown the variation of the hydrogen gas production after the expression studies.

Anticancer studies by MTT assay and flow cytometric apoptosis analysis for the isolated bacteria against the breast cancer cell lines SKBR3 and MDA-MB231. Laboratory Skills developed during my research project:

Microbial techniques like isolation of bacteria by culturing in the enriched media and by spreading, pouring by Para film overlay method, gram staining.

Biochemical techniques for the carbohydrates, protein and indole acetic acid and also the total amino acid content in the isolated bacteria by using HPLC.

Molecular biology and rDNA technology- DNA isolation, SDS-PAGE, Restriction digestion, PCR, Sequencing, cloning, transformation, agarose gel electrophoresis, and MTT assay and cell culture studies, etc.

Bioinformatics- BLAST,FASTA and Soft wares like MEGA 7,Origin for statistical analysis and graphical representation and NCBI deposition of the identified strains for the obtaining the accession numbers.

Special Skills: Isolation and synthesis of nanoparticles from the various plant sources and published the publications on the synthesized nanoparticles. WORK EXPERIENCE:

Worked as a lab technician in Sree Tirumala Medicare and DRC PVT.LTD, Kalyani Hospital, Hanamkonda from 2-8-2007 to 16-7-2009.

Working as Research Scholar in Osmania University, Biochemistry Department from 21/3/2011 to till date and also taken the Msc Biochemistry subject classes in the Department. LIST OF PUBLICATIONS (UGC Approved Journals) :

Green Synthesis of plant-mediated silver nanoparticles using Withania somnifera leaf extract and evaluation of their anti-microbial activity -Veera babu Nagati,, Rama Koyyati, Rajkiran Banala Jahnavi Alwala,, Manisha R Donda, Pratap Rudra Manthur Padigya* Department of Biochemistry, Osmania University, Hyderabad,IJAR Volume 1,Issue 9,307-313.

Green Synthesis and characterization of Silver nanoparticles from Cajanus cajan leaf extract and its antibacterial activity Veera babu Nagati,Rama Koyyati, Manisha R Donda, Jahnavi Alwala, Karunakar Rao Kundle, Pratap Rudra Manthur Padigya* Department of Biochemistry, Osmania University, Hyderabad International Journal of Nanomaterials and Biostructures Universal Research Publications – 2012,Vol 2,Issue 3,page no 39-43.

Biofabrication of silver nanoparticles using Cuminum cyminum through microwave irradiation Karunakar Rao Kudle, Manisha R Donda, Jahnavi Alwala, Rama Koyyati, Veerababu Nagati, Ramchander Merugu, Y.Prashanthi,M.P.Pratap Rudra*.-2012, 2(4)65-69.

Biosynthesis of Silver Nanoparticles using Catharanthus roseus Leaf extract and antimicrobial activity,Veerababu Nagati,Rama Koyyati,Manisha R Donda,Jahnavi Alwala,Pratap Rudra Manthur Padigya*,2013,Sep 11 2013.

Synthesis of silver nanoparticcles using extracts of Securinega luecopyrus and evaluation of its antimicrobial activity. Manisha D.R1, Jahnavi Alwala1, Karunakar Rao Kudle1, Anila Miryala,B Sreedhar and M.P.Pratap Rudra*,INT J CURR SCI,7:E 1-8.

Synthesis of silver nanoparticles from Phyllanthus reticulates: An investigation on the effect of broth (leaves & root) concentration in reduction mechanism and particle size., Karunakar Rao Kudle1 Manisha D.R1,Jahnavi Alwala1,Madhukar Rao Kundle,b Sreedhar,, M.P.Pratap Rudra* World Journal Of Pharmacy and Pharmaceutical Sciences,Volume 2,Issue 5,2839-2849.

Biosynthesis of Silver Nanoparticles using Flower Extracts of Catharanthus roseus and evaluation of its antimicrobial activity,Manisha D.R1, Jahnavi Alwala1, Karunakar Rao Kudle1, M.P.Pratap Rudra*2014, World Journal Of Pharmacy and Pharmaceutical Sciences,Volume 3,Issue 5,877-885.

Interpretative in vitro phytochemical analysis, Synthesis of silver nanoparticles and Thin Layer Chromatography screening of Aqueous and Ethanolic extracts of Morinda citrifolia L.

(Noni) Fruit and their comparative study. Jahnavi Alwala,Manisha R Donda,M.P.Pratap Rudra*,in World Journal Of Pharmacy 2014.

Photoproduction of hydrogen by Vishakahaptnam photosynthetic bacteria and their effect on cultural conditions Jahnavi Alwala1, M.P.Pratap Rudra *1, Ramchander Merugu, in International journal Of Research (IJR) Vol 1, Issue 8, September 2014.

Impact of various cultural conditions on photo production of hydrogen from Kanyakumari Photosynthetic bacteria Jahnavi Alwala 1,M.P.Pratap Rudra *1,Ramchander Merugu, in International Journal of Multidisciplinary Research and Development Volume 1,Issue 7,Part I 2014.

Biosynthesis of Silver Nanoparticles using Impatiens balsamina leaf extracts and its characterization and cytotoxic studies using human cell lines Raju Nalvothula,Jahnavi Alwala,Veerababu Nagati and M.P.Pratap Rudra*,in International journal of Chemtech Research Vol 7,No.5 pp 2460-2468.

Prevalence of distinctive sources on Hydrogen Production from Goa Photosynthetic Bacteria. Jahnavi Alwala1, M.P.Pratap Rudra*1, Ramchander Merugu2. JECET; March 2015- May 2015; Sec. A; Vol.4.No.2, 519-525.

Isolated phototrophic bacterial pure strains from marine water samples were deposited in NCBI as Rhodopsuedomonas faecalis SMR JVI (Vishakapatnam marine water), Rhodopsuedomonas faecalis MPPR 001(Kanyakumari marine water) and Rhodopsuedomonas palustris MPPR 001(Goa marine water).

Cytotoxicity of Biologically Synthesized Silver Nanoparticles from Citrus Lemon against some Cell Lines Raju Nalvolthula1, Ramchander Merugu2, Jahnavi Alwala1, M.P.Pratap Rudra1 * International Journal of PharmTech Research (Vol.8, No.4, pp 691-695, 2015).

Editorial board member for the Journal – Journal of Journal of Pharmaceutical and Biological Sciences (Ref ID: JPABS/EB/052) from 28-12-2016. References:

Dr.M.P.Pratap Rudra,

Retired Professor,

Department of Biochemistry,

University College of Science,

Osmania University, Hyderabad. Mobile: 939-***-****, Email id:


Retired Assistant Director,

Drugs Control Administration,

Telangana, Hyderabad. Mobile: 944*******.

Email id:


Retired Scientist,

Telangana Pollution Control Board,

Sanathnagar, Hyderabad. Mobile: 961-***-****.



Father name : VenuPrasad Alwala.

Date of birth : 26-08-1987.

Languages known : English, Telugu, Hindi.

Sex : Female

Marital status : married

Hobbies : Reading books and Painting.

Strengths : Hardworking, team work.

Nationality : Indian

Address : Flat No: 402, Vishwa Plaza, Road no: 7A, Bhandari Layout, Nizampet, Hyderabad - 500 090.

Phone No: 995-***-****(Father).

In view of the above I request you to kindly give an opportunity to serve your esteemed organization. I shall be thankful and grateful to you. Thanking you,




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