Plant Project

GEETIKA GAMBHIR

B **First floor

Inderpuri,

New Delhi 110012

Email: *********@*****.***

CAREER OBJECTIVES:

Improve my potential through on job learning and by solving problems/ challenges faced in an organization. Contribute along with the organization’s team to its goals to the best of my potential.

PROFESSIONAL QUALIFICATION

DOCTORATE

Degree Doctorate Degree in Biotechnology

Title of thesis Studies on Agrobacterium-mediated insect resistance gene transfer in cabbage (Brassica oleracea L. var. capitata) and molecular analysis of regenerated plantlets.

Institute Dr Y. S. Parmar University of Horticulture and Forestry,

Nauni, Solan, 173230 (H.P.)

Major field Biotechnology

Minor field Microbiology and Biochemistry

Division First

POST GRADUATION

Degree Masters Degree in Biotechnology

Title of thesis In vitro production of bulblets in Lilium by anther and ovary culture

Institute Dr Y. S. Parmar University of Horticulture and Forestry, Nauni, Solan, 173230 (H.P.)

Major field Biotechnology

Minor field Genetics and Floriculture

Division First

GRADUATION 2007

Degree Bachelors Degree in Biotechnology

Institute DAV College Amritsar

Affiliated to Guru Nanak Dev University

Division First

EDUCATION:

Class

Year

Institute

Board/ University

Percentage Secured

10th

2002

DAV Public school,

Vikas-puri

CBSE

77.4%

10+2

2004

DAV Public school,

Vikas-puri

CBSE

78.6%

BSc Biotechnology

2007

DAV College Amritsar (Pb)

Guru Nanak Dev University

75%

MSc

Biotechnology

2010

Dr Y.S. Parmar University of Horticulture and Forestry, nauni

Dr Y S Parmar University of Horticulture and Forestry Nauni (Solan) HP

78%

PhD Biotechnology

March,

2014

Dr Y.S. Parmar University of Horticulture and Forestry, nauni

Dr Y S P UHF Nauni (Solan) HP

78%

LANGUAGES KNOWN: Hindi, English and Punjabi.

ACHIEVEMENTS:

I have got Certificate of Merit in my Graduation in DAV College Amritsar (Pb).

Participated in Badminton and Chess competitions at School Level.

TECHNIQUES KNOWN

Genomics

DNA, RNA (Total, Small) Isolation, Plasmid Isolation (Mini, Midi & Maxi Prep), PCR (Polymerase Chain Reaction) and RT PCR, Gene Cloning, Creation of Vector constructs using various cloning Techniques, Southern and Northern Blotting, Probe Radiolabeling, Real-Time PCR

Plant Science

Agrobacterium mediated genetic transformation, Vaccum infiltration assisted genetic transformation, Centifugation assisted genetic transformation, Tissue culture and transformation of Lilium, Cabbage, Poplar, Wheat and Tobacco. Bioassay.

Microbiology

Maintenance of bacterial cell cultures, Sub-culturing of cell line, Cryopreservation and revive the cell, Disc diffusion assay, Minimum Inhibition Concentration (MIC).

BELIEFS:

I strongly believe that there is no task which is impossible.

Hard Work of a person finds silver lining among each dark cloud.

Practice makes a man perfect.

RELATED SKILLS:

Creative ideas

Lead others by setting example

Good communication skills

Perfectionist approach to tasks

Efficient Decision making

STRENGTHS:

Confident and self-motivated

Quick learner

Strong Determination and a good foresight

Positive and optimistic

Factual and objective

Perceptive and sensitive

High energy, drive, and ambition

PROJECT OR TRAINING EXPERIENCE:

Training for one month in ESCORTS HEART AND SUPER SPECIALITY INSTITUTE Amritsar (Pb) and completed project work.

Participated in one day Training-Cum-Awareness Programme on Protection of Plant Varieties held at Dr Y S Parmar UHF Nauni Solan on 20th July, 2012.

ABSTRACT PUBLISHED

Aggarwal G, Gambhir G and Srivastava D.K. Bio management studies to control agrobacterial growth and enhance transformation frequency in himalayan poplar (Populus ciliata wall.) at International conference on Plant Biotechnology for food security: New Frontiers at National Agricultural Science Centre, PUSA, New Delhi on 21st Feb to 24th Feb 2012.

Gambhir G, Kumar P and Srivasatava D K. Effect of kanamycin and cefotaxime on cultured tissues of cabbage (Brassica oleracea L. var. capitata) and its role in genetic transformation studies at International conference on industrial biotechnology at Patiala Agriculture University, Patiala, Punjab on 21st Nov to 23rd Nov 2012.

Sharma S, Gambhir G, Sharma P and Srivastava D K. Plant regeneration and genetic transformation studies in Pea (Pisum sativum L.) tissues at National conference on Perspective & trends in plant sciences and biotechnology at department of Botany, Panjab University, Chandigarh on 21st Feb to 23rd Feb 2014.

Sharma P, Sharma P, Gambhir G and Srivastava D K. Plant regeneration, genetic transformation and expression of foreign gene in lettuce (Lactuca sativa L.) at National conference on Perspective & trends in plant sciences and biotechnology at department of Botany, Panjab University, Chandigarh on 21st Feb to 23rd Feb 2014.

Sharma P, Gambhir G, Gaur A And Srivastava D K. Effect of TDZ (thidiazuron) on the regeneration potential of hypocotyl and cotyledon explants of broccoli (Brassica oleracea L. Var. italica) at National conference on Perspective & trends in plant sciences and biotechnology at department of Botany, Panjab University, Chandigarh on 21st Feb to 23rd Feb 2014.

Sharma S, Gambhir G and Srivastava D K. Molecular characterization of lettuce (Lactuca sativa L.) genotypes using RAPD PCR at National conference on Perspective & trends in plant sciences and biotechnology at department of Botany, Panjab University, Chandigarh on 21st Feb to 23rd Feb 2014.

Verma H, Gambhir G and Srivastava D K. Genetic transformation studies in Broccoli (Brassica oleracea L. Var. italica) with npt-II and Gus genes at National conference on Perspective & trends in plant sciences and biotechnology at department of Botany, Panjab University, Chandigarh on 21st Feb to 23rd Feb 2014.

PUBLICATION:

Kumar P, Gambhir G and Srivastava D.K. 2017. "Transgenic broccoli expressing The insect resistance gene (cryIAa) resistant to Diamondback moth (Plutella xylostella)". Plant and Soil Office. Submission ID: PLSO-D-17-00414.

Gambhir G, Kumar P and Srivastava D.K. 2016. The stimulatory effect of antibiotics on different tissues and its significance in genetic transformation of cabbage (Brassica oleracea L.var. capitata) – an economically important vegetable crop. Journal of Argiculture Science and technology. Manuscript ID: JAST-1607-12241.

Gambhir G, Kumar P and Srivastava D.K. 2016 "High frequency regeneration of plants from cotyledon and hypocotyl cultures in Brassica oleracea cv. Pride of India". Biotechnology Reports. S2215-017X(17)30049-8 DOI: http://dx.doi.org/doi:10.1016/j.btre.2017.02.005.

Reference: BTRE 193

Sharma S, Kumar P, Gambhir G, Kumar R and Srivastava D.K. 2016. Assessment of genetic diversity in lettuce (Lactuca sativa L.) germplasm using PCR- based molecular markers. Journal of Agricultural Science and Technology. Manuscript ID: JAST-1603-11629.

Verma H, Kumar P, Gambhir G and Srivastava D.K. 2016. Agrobacterium-mediated transformation of broccoli (Brassica oleracea L. var. italica), an important vegetable crop with gus and npt-II genes. Crop Improvement. 41(2): 139-147

Sharma S, Gambhir G, Sharma P and Srivastava D.K. 2016. Plant regeneration and Agrobacterium-mediated transformation of hypocotyl and root explants of pea (Pisum sativum l.) by npt-ii and gus gene. Journal of Cell and Tissue Research Vol. 16(1). ISSN: 0973-0028; E-ISSN: 0974-0910.

Manuscript number : CBAB-2015-0759 in Crop Breeding and Applied Biotechnology

Kumar P, Gambhir G, Gaur A and Srivastava D K. 2015. Molecular analysis of genetic stability in in vitro regenerated plants of broccoli (Brassica oleracea L. var. italica). Current science 109(8): 1470-1475.

Gambhir G and Srivastava D K. 2015. Thidiazuron Induces High Frequency Shoot Regeneration in Leaf and Petiole Explants of Cabbage (Brassica Oleracea L. Var. Capitata). Journal of Biotechnology and Biomaterials 5(1): 1-6.

Sharma S, Gambhir G and Srivastava D K. 2014. High frequency organogensis in cotyledon and hypocotyl explants of cabbage (Brassica oleracea L.var. capitata). National Academy Science Letters 37(1): 5-12.

Kumari A, Gambhir G and Srivastava D K. 2014. Plantlet regeneration from cotyledon and hypocotyl explants of brinjal (Solanum melongena L.).

Kumari A, Gambhir G and Srivastava D K. 2014. Plant regeneration and Agrobacterium-mediated gene transfer in brinjal (Solanum melongena L.). Vegetable Sciences.40(2):143-148.

Sharma S, Kaur M, Srivastava D K and Gambhir G. 2014. In vitro evaluation of plant growth regulators on tissue culture bioassay produced by Pseudomonas species. International journal of Agriculture, Environment and Biotechnology 7(4): 747-757.

Kumar P, Gambhir G and Srivastava D K. 2014. Molecular analysis of genetic stability in in vitro regenerated plants of broccoli (Brassica oleracea L. var. italica) using RAPD markers. Journal of Crop Improvement. In Press.

Gambhir G, Kumar S and Sen S.2010. An Improved Regeneration System of Oriental Lily hybrids from Ovary-Ovule Culture Using Plant Growth Regulators. Floriculture and Ornamental Biotechnology.4(1): 1-4.

BOOK CHAPTER

Srivastava, D.K., Gambhir G. and Sharma P. 2013. “Plant Cell and Tissue Culture Techniques in Crop Improvement”. In the book “Biotechnology in Agriculture and Food Processing: Opportunities and Challenges” Edited by Paramjit S.Panesar and Satwinder S. Marwaha, CRC Press Taylor and Francis Group, London, Newyork, pp. 75-119.

Bioprospecting of Genes from Microbes for Stress Management in Agricultural Crops (2016). Shashi Shekhar, Geetika Gambhir and Jasdeep Padaria.

Srivastava D.K, Kumar P, Sharma S, Gaur A and Gambhir G. 2016. Genetic engineering for insect resistance in economically important vegetable crops". In the book “Plant Tissue Culture: Propagation, Conservation and Crop Improvement” published by an International Publisher ‘Springer”, pp. 343-378. DOI 10.1007/978-***-**-****-3_15.

REFERENCES

Prof. D.K.Srivastava

(Former Head)

Dept.Of Biotechnology

College of Horticulture,

Dr Y.S. Parmar University of Horticulture and Forestry

Nauni, Solan, India, 173230

Contact No. +91-941*******

Email: ********@*****.***

Dr. M.kaur

Professor (Microbiolgy)

Dept. of Basic Sciences

College of Forestry,

Dr Y.S. Parmar University of Horticulture and Forestry

Nauni, Solan, India, 173230

Contact No. +91-179*-******

Email: ************@*****.**.**

Dr. K. Alagusundaram

Deputy Director General (Engineering)

Indian Council of Agriculture Research

Krishi Anusandhan Bhavan-II

Pusa, New delhi-110012

Contact No.: 91-11-258*-****

Email: *******@****.***.**

WORK EXPERIENCE:

S. No.

Name of the institute

Project Title

Post held

Time Period

From

To

1.

Dr Y.S. Parmar, University of Horticulture & Forestry, Nauni, Solan (HP)

“Studies on Agrobacterium-mediated genetic modification of lignin biosynthesis gene in Himalayan Poplar (Populus ciliate wall) for better paper production .”

Project Fellow

23rd

Jan 2013

3rd Nov 2013

2.

National Resarch Centre for Plant Biotecnology

“National Initiative on Climate Resilient Agricultre

Resarch associate

2ndFeb,2015

20thFeb, 2016

3.

KAB-II, ICAR

“Agriculture foresight development centre”

Senior Research Fellow

28th March, 2016

27th March, 2017

JOBS I CAN HANDLE

Genetic transformation, Molecular biology, Tissue culture and Karyotype work.

DUTY EXPERIENCE

Given two days Shoolini Fair duty on 22nd and 23rd June, 2012 at Thodo Ground, Solan so as to explain the exhibits to the visitors.

PERSONAL INFORMATION:

Husband’s Name: Mr. Shobhit Chopra

Permanent Address:

B 49 First Floor, Side Entry

Inderpuri

New Delhi 110012

Contact No.: 995-***-****

Age: 29 years

Nationality: Indian

Marital Status: Married

Hobbies: Dancing, Playing, Singing

Aptitudes: Sincere, Focused, Analytical, Hardworking, and friendly

Date: 27.03.2017

Place: New Delhi (Geetika Gambhir)

Contact this candidate