Anirudh Chowdhary Page * of *

ac4i9w@r.postjobfree.com +1-616-***-****

OBJECTIVE: To obtain a responsible and challenging position to contribute my knowledge and skills for growth of the company.

EDUCATION:

Grand Valley State University, Allendale, Michigan - USA (2010- 2012) Professional Science Masters in Cell and Molecular Biology: Biotechnology emphasis. GPA: 3.65/4.0 Key Skills: DNA/RNA extraction/purification, restriction digestion, plasmid manipulation and protein purification, animal and plant tissue culture, PCR, RT-qPCR, bacterial cell culture, electrophoresis, western blotting, cryopreservation, design protocols, setup experiments, maintain lab note books and equipments. KLE’S College of Engineering and Technology, Belgaum, Karnataka - India (2004-2008) Bachelor of Engineering in Biotechnology

1st position in academics for department and college for the year 2007-08 and top in 2% throughout undergraduate studies.

Recognized as ‘Best student’ department of Biotechnology, KLE Society’s College of Engineering and Technology for overall outstanding academic achievement, participation in co-curricular activities. BIOTECHNOLOGY EMPLOYMENT

Tetra Discovery Partners Inc., Grand Rapids, Michigan, October 2012-present Research Biologist/Lab Manager

Part of research team to effectively take a drug molecule into PhaseII clinical trials for Alzheimer’s disease.

To check the inhibitory effect of various new drugs against different isoforms of phosphodiesterase 4.

Perform enzyme (IC50) assays in 96 well plates as per SOPs, operate microplate spectrophotometer, troubleshoot assays, data collection and statistical analysis, report writing, database entry.

Lab manager, ordering lab supplies, maintain inventory, connect with new vendors, negotiate for best price schedule preventive maintenance, calibrate and troubleshoot equipment.

Carry out serial dilution of drug molecules and enzymes to determine effective drug concentration

Data analysis and interpretation using CDD (Collaborative Drug Discovery) database Portal.

Generate, analyze and modify database (CDD) protocols as per requirements by PI’s/ research team.

Analyze real time data for more than 2000 novel compounds for PE4D inhibition using CDD and GraphPad Prizm. Carry out enzyme titrations, enzyme inhibition assays, and SDS PAGE.

Grow and maintain cultures of THP-1, Ramos (RA1) and Ramos.2G6.4C10 cells and perform Cell Proliferation Assays (MTS assays).

3H rolipram binding assays to determine specific binding of test compounds to PDE4 enzymes.

Perform solubility assays and formulations for mice dosing for novel compounds. Grand Valley State University, Grand Rapids, Michigan, January 2012- April 2013 Independent study

Investigate the effects of the drug BIBR1532 and related analogs on Telomerase activity in human Prostate Cancer cells using Telomerase detection kits by means of TRAP (Telomeric Repeat Amplification Protocol) assay. Grow and maintain cultures of PC3 cell lines for PC3 parental, 5V and 17V.

Discuss and interpret results, assist PI and lab members in various research methods/techniques. Michigan State University, Department of Obstetrics, Gynecology & Reproductive Biology, Grand Rapids, Michigan, August 2011 – June 2012

Internship

Assist primary investigators to determine the roles of 17βHSD7 gene on estradiol synthesis in endometriosis in female reproductive system.

To determine the expression of 17HSDB7 in baboon model and human samples for endometriosis using Real Time PCR, ELISA and Immunohistochemistry.

To determine the expression of estrogen receptors on Klonisch cells and Genotyping. Grand Valley State University, Grand Rapids, Michigan, August 2010- May 2012 Graduate Assistant

Grow and maintain soy bean plants for various treatments by nucleic acids lab students.

Supervise students in nucleic acids lab with extraction and purification of DNA, developing primers using SDSC Biology workbench and DNA STAR soft wares, PCR amplification and agarose gel analysis. Institute of Computational Biology, India, August 2008-December 2009 Research Assistant and Business Development Executive

Involved in creation of Appetite Regulating Compound Database (ARCD) which is available online at www.iocb.in. It is a repository for appetite regulating compounds, both inducers and suppressors. These compounds can be tested and regulated to control obesity.

Assist professors in their research and communicate with prospective students.

Advertising and marketing of courses offered by meeting faculty and students of various biotechnology institutes.

Organize workshops, guest lectures, give presentations and prepare class schedules. ACADEMIC PROJECTS

Protocol for working on Autodock 2.4 software (Team size:1), January-April 2010 Design step wise instructions to carryout docking of 2D1R (Luciferase) molecule using Autodock 4.2 software. Protocol is to be used by students in Advanced Computational Biology course.

Structure Based drug Designing (Team size:3), Final year 2007-08 Carry out docking studies for Curcumin and other commercially available drugs such as Imanitib, Gemacitabine, Flourocil and Quinazoline with Aurora A kinase 1MUO protein structure using Argus lab in pancreatic cancer studies. Curcumin with an E-value of -10.95 forms better drug than other commercially available drugs for pancreatic cancer.

Production of Biodiesel from soy bean oil (Team size:3), Pre-final year 2006-07 Design parameters and construct a working model for biodiesel production from soy bean oil involving processes of transesterification. We produced biodiesel of low calorific value that needed further purification.

Construction of a working Bioreactor (Team size:2), Pre-final year 2006-07 Design parameters to construct a stirred tank continuous flow reactor using standard values of dimensions for shaft, rotor, baffles and tubing, using 1.5mm thick steel strips. We constructed a fully functional bioreactor with probe attachments for pH, temperature and rpm control. RESEARCH AND LAB EXPERIENCE

Mark E. Gurney, Anirudh Chowdhary et al. (2017). “Memory Enhancing Effects of BPN14770, an Allosteric Inhibitor of Phosphodiesterase-4D, in Wild-type and Humanized Mice”- Submitted - Nature Chem Bio

Presented poster on “Investigating the Effects of BIBR1532 and Related Analogs on Telomerase Activity in Human Prostate Cancer Cells (PC3-Parental)”, American Society For Cell Biology, San Francisco, California, December 2012.

Poster presentation “Expression of 17 Beta Hydroxysteroid Dehydrogenase Type 7, a Key Enzyme in Estradiol Biosynthesis, is Highly Expressed in Cell Specific Manner in Endometriosis” at Endo 2012 annual meeting at Houston, Texas, June 2012.

Poster presentation “Investigating the Effects of BIBR1532 and Related Analogs on Telomerase Activity in Human Prostate Cancer Cells” for Students Scholarships Day at Grand Valley State University, Allendale, Michigan, April 2012.

Paper and poster presentation “Use of Antioxidants as chemopreventive Agents: An Insilco Approach” at an International Conference on Free Radicals held by SFRR(Society for Free Radical Research) at Aligarh Muslim University, Aligarh, India, March 2009.

Poster presentation “Phenol Degradation by Pseudomonas Species” at National level symposium held at Adiyaman college of Engineering and Technology, TN, India, April 2006.

Poster presentation “Production of Biodiesel from Soy bean oil” at a national Level symposium

“PARICHAY” held at K.L.E.S’s College of Engineering and Tech, India, February 2005. Page 2 of 3

WORKSHOPS ATTENDED

“Genomics and discovery of Therapeutic drugs” GVSU, a hands on workshop, Allendale, Michigan, 2012.

“Entrepreneurship Awareness” Conducted by Visvesvaraya Technological University (VTU), Belgaum, India, 2008.

“Genetic Engineering Techniques” a hands on work shop at Pooja Bhagwat Memorial Mahajana Post Graduate center, Mysore, India, 2005.

“Clinical Research and Database Management” at, K.L.E Society’s College Engineering and Tech, 2007. PROFESSIONAL SKILLS

Life Science

Laboratory experience in, nucleic acids extraction and quality analyses, DNA/RNA sequence analysis, restriction digestion, plasmid manipulation, PCR analyses (Real Time and Reverse Transcriptase), gel electrophoresis, sterile cell and tissue culture, cell counting, ELISA, tissue embedding and sectioning using microtome, Immunohistochemistry, Immunocytochemistry, Western blot, Southern blot and hybridization, scratch/wound assay, cell proliferation assay(MTS assay), handling mice to administer compound solutions orally and carryout genotyping from mice tail clippings, handling research microscopes like bright field, DIC & fluorescence and fluorescent dissecting scopes, UV/Vis Spectrophotometer, pH meter, HPLC, SDS-PAGE, column, paper and affinity chromatography, fermentation/ bioprocess technology and microbial biotechnology. Making reagents and solutions, maintaining lab note books and ordering supplies, writing manuscripts, carrying out literature reviews, setting up experiments, maintaining and calibrating lab instruments, analyzing, interpreting and presenting experimental progress data and results in lab meetings.

Bioinformatics

Docking and visualization softwares: Autodock 4.2, Argus lab, ChemSketch, VMD, SwissDock.

Data mining: Pub Med, Protein Data Bank, GenBank, Web of Science, BLAST and Bioconductor. Computers

Operating systems: Windows 9x/2000/XP/Vista/7/8, Mac.

Microsoft office: Word, Excel and Power point, Access, Outlook, RefWorks, Mendeley

Statistical software: PASW (SPSS), Base SAS

Computer Languages: C, Perl

Managerial skills

Proficient in lab set up and management.

Excellent verbal and written communication, problem solving, organization and analytical skills.

Hard working and team player and can also work independently with minimal supervision.

Enthusiastic and swift in learning new things and excellent time management skills. LEADERSHIP/ RECOGNITION

‘Promotional officer’ of the Life Sciences and Biotechnology (LAB) student organization. Grand Valley State University, Allendale, Michigan, August 2010- April 2012.

Secretary “BEATS (Biotechnology Engineers Association Of Teachers and Students)”, 2007-08. Department of Biotechnology, K.L.E Society’s College of Engineering and Technology, India.

Cultural Secretary, K.L.E Society’s College of Engineering and Technology, India, 2007-08.

Secured 2nd Place in Biomodelling at National Level Symposiums “PARICHAY” 2006 and “SYNAPSE” 2007 held at K. L. E. Society’s College of Engineering and Technology, India. Page 3 of 3

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