Project Manager Microsoft Office

Chiara Montemurro, Ph.D.

Dept. of Medicine, Section of Endocrinology 11692 Chenault St. #303

David Geffen School of Medicine Los Angeles, CA 90049 University of California, Los Angeles Phone: 310-***-**** Los Angeles, CA 90095 ac4few@r.postjobfree.com Phone: 310-***-****

PROFILE

Ph.D. level scientist with 8 years of experience in islet and diabetes research

Active leadership in different research projects involving collaborations with other professionals

Prolific independent researcher with strong problem-solving and communication skills

Extensive experience in molecular and cell biology techniques

Successful set-up, management, and execution of animal studies for evaluation of biological targets for drug development

Desire to learn new techniques and flexibility in investigating scientific problems using various approaches has led to proficiency in a wide range of laboratory skills

Molecular biology – DNA, RNA isolation; RT-PCR; protein extraction and subcellular fractionation

Biochemistry – Quantitative immunoblotting; immunohistochemistry and immunofluorescence; flow cytometry; immunoprecipitation; ELISA; spectroscopic and fluorometric assays; insulin secretion assay; metabolic analysis

Cell and tissue culture – INS 832/13, rMC-1, HPDE, RIN-5F, HEK-293 and HTB9 cell lines; rodent and human islet and primary beta cell culture; adenoviral and lentiviral-mediated overexpression of genes; siRNA-mediated gene silencing

Animal models – Mouse and rat handling, pancreas and islet isolation, electroretinography, rodent models of type 1 and type 2 diabetes; intraperitoneal and subcutaneous injection; transcardial perfusion; extraction and dissection of rodent pancreas and liver; tissue processing and fixation

Microscopy – Fluorescent and confocal microscopy and morphometrical analysis

Teaching and mentoring – Training undergraduate students in laboratory techniques and islet and retinal cell biology

RESEARCH EXPERIENCE

University of California, Los Angeles, Post Doctoral Fellow (Feb. 2015 – Present) Advisor: Peter C. Butler

Demonstrated mitochondrial dysfunction and upregulation of Hif1a-PFKFB3 pathway in the hIAPP

(human islet amyloid polypeptide) rat model of type 2 diabetes

Investigation of strategies to abolish the metabolic changes caused by hIAPP overexpression in beta- cells

Developed a synchronization protocol to study glucose metabolism, mitochondrial dynamics and intracellular calcium levels in INS832/13 during the cell cycle

Found inflammatory molecules, released by islets under stress, promote ductal proliferation that is linked to the increased risk for pancreatitis in type 2 diabetes

Supervision of undergraduate students and a technician

Management of animal studies to test the efficacy of drugs in the treatment of type 2 diabetes This work resulted in two pubblications in Cell Cycle and JCI Insight. One manuscript is under review and another one in preparation.

State University of Campinas, Brazil Post Doctoral Fellow (Apr. 2013 – Dec. 2014) Advisor: Jose Butori Lopes de Faria

Found autophagic flux is dysregulated in retinal Muller cells under high glucose concentration and is responsible for cell death

Utilization of UTAS-E3000 system to record electroretinograms in rats

Found matrix-free NALDI-MS (nano-assisted laser desorption-ionization mass spectrometry) imaging provides well-resolved images of distribution of low molecular weight drugs in kidney tissue

Demonstrated podocyte apoptosis is prevented in streptozotocin diabetic rats by green tea treatment

Supervision of undergraduate students and a research technician This work resulted in three pubblications in IOVS, J Nutr Biochem and Drug Test Anal. University of Verona, Italy PhD Student (Oct. 2008 – May 2012) Advisor: Enzo Bonora

Demonstrated insulin released by insulinomas does not have any effect on beta cell turnover in tumor- adjacent tissue in patients with the disease

Found amyloid deposition is a characteristic of islets of type 2 diabetic patients and not patients with adenocarcinoma or pancreatitis

Involvement in studies evaluating beta-cell function in patients with type 2 diabetes who underwent oral glucose tolerance test

Initiated a productive collaboration with Dr. Meier at University of Bochum, Germany This work resulted in one pubblication in Regul Pept. University of Padova, Italy Graduate Student (Mar. 2007 – Apr. 2008) Advisor: Gian Paolo Rossi

Demonstrated green tea extracts reduce collagen deposition in the heart of a rat model of hypertension treated with high dose of angiotensin II

Collaboration in a study evaluating the activity of Lp-PLA(2) (lipoprotein-associated phospholipase A(2)) in twins

University of Padova, Italy Graduate Student (Jun. 2004 – Aug. 2004) Advisor: Giovanni Abatangelo

Demonstrated hyaluronan-based biomaterial is effective to promote replication and differentiation of preadipocytes derived from aspirated human adipose tissue EDUCATION

University of Verona, Italy (Oct. 2008 – May 2012)

Ph.D., Medical, Clinical and Experimental Science

Thesis: Pancreatic beta cells turnover: effects of endogenous hyperinsulinemia and amyloid deposits University of Padova, Italy (Oct. 2005 – Dec. 2007)

M.S. in Medical Biotechnology

Thesis: Cardiovascular morphological and molecular effects of interventions on oxidative stress in a hypertensive rat model

University of Padova, Italy (Oct. 2001 – Dec. 2004)

B.S. in Biotechnology

Thesis: Isolation and culture of human preadipocytes on hyaluronic acid - based biomaterials POST DEGREE PROGRAMMES AND COURSES

XVI School of Pure and Applied Biophysics, Italy (Jan. 2012) Multimodal Methods for Cell Imaging and Tracking

University of Padova, Agripolis, Italy (Dec. 2004 – Aug. 2005) Agro-alimentary Biotecnology Master Degree courses COMPUTER SKILLS

Windows, Linux (Ubuntu) and Macintosh OS

Microsoft Office

Adobe Photoshop, ImageJ, ImagePro, ZEN, and other imaging software

Statview, GraphPad Prism

FELLOWSHIPS

NIH National Institute of Health, USA (Feb. 2015 – present) Role: Post-doc Fellow

FAPESP Fund. de Amparo a Pesquisa do Estado de Sao Paulo, Brazil (Apr. 2013 – Dec. 2014) Role: Post-doc Fellow

Ministry of Education and Research, Italy (Oct. 2009 – May 2012) Role: Ph.D. Student

ESU Regional Authority for Study Right of Padova, Italy (Oct. 2005 – Dec. 2007) Role: M.S. Student

ESU Regional Authority for Study Right of Padova, Italy (Oct. 2001 – Dec. 2004) Role: B.S. Student

HONORS, AWARDS, CERTIFICATION

Second place poster prize, DOM Research day, Los Angeles, USA (Oct. 2016)

Cover paper, Drug Testing and Analysis, Vol. 6 (Sep. 2014)

Travel award, EASD European Association for the Study of Diabetes (Sep. 2014)

Italian Professional Biologist Certification (Sep. 2012) PROFESSIONAL AFFILIATIONS

EASD European Association for the Study of Diabetes, Member

Italian Professional Biologist, Member

SCIENTIFIC PUBLICATIONS

Montemurro C, Kohler C.U., Uhl W, Tannapfel A, Schmidt WE, Bonora E, Meier JJ. Endogenous hyperinsulinaemia in insulinoma patients is not associated with changes in beta-cell area and turnover in the tumor-adjacent pancreas. Regulatory Peptides, 165(2-3): 180-5, 2010 Tata A, Montemurro C, Porcari A, Silva K, Lopes de Faria J, Eberlin M. Spatial distribution of low MW drugs in tissues via matrix-free NALDI-MS imaging. Drug Testing and Analysis, 6(9): 949-52, 2014 E M P Prado, A Papadimitriou, D A T Teixeira, C Montemurro, D Andreazzi Duarte, J M Lopes de Faria, J Lopes de Faria. Reduced LRP-6 expression and increase in the interaction of GSK3β with p53 contribute to podocyte apoptosis in diabetes mellitus and are prevented by green tea. Journal of Nutritional Biochemistry, 26(4): 416-30. 2015

Lopes de Faria JM, Duarte DA, Montemurro C, Papadimitriou A, Consonni SR, Lopes de Faria JB. Defective autophagy in diabetic retinopathy. Invest Ophthalmol Vis Sci, 57(10): 4356-66, 2016 B Schludi, AS Md Moin, C Montemurro, T Gurlo, A Matveyenko, D Kirakossian, D Dawson, S Dry, P Butler and A Butler. Islet inflammation and ductal proliferation, a link to pancreatitis in type-2 diabetes?. JCI Insights, 2(13), 2017

Montemurro C, Vadrevu S, Gurlo T, Butler A, Vongbunyong KE, Shirihai O, Satin L, Braas D, Butler P, Tudzarova S. Cell cycle related metabolism and mitochondrial dynamics in pancreatic beta-cell. Cell cycle, 1- 14, 2017.

Montemurro C, Vongbunyong KE, Vadrevu S, Gurlo T, Butler A, Ritou E, Satin L, Shirihai O, Braas D, Butler P and Tudzarova S. Rescue of β-cell viability by targeting PFKFB3 in presence of misfolding protein stress. Under review Nature

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