CURRICULUM VITAE

April **th, ****

NAME: Soon Cheon Shin

Research Associate, Division of Allergy and Immunology Northwestern University Feinberg School of Medicine Home Address: 2748 Wilshire Lane, Northbrook, IL 60062 Home Phone: 847-***-****

Business Address: 240 E. Huron St. McGaw M-215, Chicago, IL 60611 Business Phone: 312-***-****

Fax: 312-***-****

E-mail: ac477a@r.postjobfree.com

Citizenship: US citizen

Summary of Experience

Over 20 years of research experience across one industry and two universities

7 years of experience in Laboratory Management

14 years of experience in pre-clinical sample handling and data management

Extensive experience in biology, cancer immunology, immunology, allergy and biochemistry

Skilled in data analysis and presentation using MS Office, Graph Pad Prism etc. General Roles and Responsibilities

Involved with all aspects of research including: planning, experimental designing, execution and data analysis

Perform data analysis using multiple statistical software and prepare results for presentations and publications

Maintain records of experimental results

Review scientific literature for to stay abreast of scientific developments, support ongoing research projects and assay for design, implementation and troubleshooting Education

College: Yon-Sei University, Korea

Major: Biology, Awarded Bachelor of Science degree Graduate School: Yon-Sei University, Korea

- Major: Bioengineering, Awarded Master of Science degree Seoul National University, Korea

- Major: Microbiology/Immunology, Awarded Doctor of Philosophy Professional Organization

Northwestern University Feinberg School of Medicine, Chicago, IL 2004. 9 ~ present:

- Research Associate, Division of Allergy/Immunology Field: Mechanisms of human siglec-8 & 6 on Eosinophils and Mast cells. Glucocorticoid regulation and sensitivity study in human Th17, etc. The Chicago Medical School. North Chicago, IL. 2000. 3 ~ 2004. 8

- Senior Research Associate, Dept. of Microbiology and Immunology Field: T/Tn antigen preparation for breast cancer immunotherapy. T/Tn antigen pulsed Immature Dendritic cell for immunotherapy study. Dong-A Pharmaceutical Co. Korea 1995. 3 ~ 2000. 2

- Senior Research Scientist, Research Institute,

Field: Development of diagnostic Kits such as HBV, HCV, HIV-1 & 2 and HCG. Laboratory Skills

Cell and Tissue Culture:

Mammalian cell line culture

Primary cell culture

Bacterial culture

T, B, Th17, DC, Mo/M culture

Molecular Biological Techniques:

DNA & RNA isolation & purification techniques

PCR, RT-PCR

Flow Cytometry

Immunological & Microbiological Techniques:

Polyclonal Ab production from animals(Rabbit, mouse and Guinea Pig)

Flow Cytometry

ELISA, RIA(51Cr release) and CBA(Cytometry Beads Assay)

Hybridoma technology: Monoclonal Ab production

Development of ELISA kits(HBV, HCV, HIV-1 & 2, HGC and AFP) Protein Purification Techniques:

Electrophoresis and Western Blot

Human Tissue and Sample Handling:

Handle infectious human blood samples

T/Tn antigen isolation from human blood for tumor vaccine preparation Animal Care and Handling

SPF, GF and conventional animal experiment: mouse, rat, porcine

Aseptic technology : GLP, GMP experience

PUBLICATIONS

A. Original Investigations:

(1) J. Banuelos, Y. Cao, S. Shin, & N. Z. Lu. Immunopathology alters Th17 cell glucocorticoid sensitivity. Allergy. (2017) Mar; 72(3):331-341.

(2) Banuelos J, Shin S, Cao Y, Bochner BS, Morales-Nebreda L, Budinger GR, Zhou L, Li S, Xin J, Lingen MW, Dong C, Schleimer RP, Lu NZ. BCL-2 protects human and mouse Th17 cells from glucocorticoid-induced apoptosis. Allergy. (2016) May; 71(5):640-650.

(3) Banuelos J, Shin SC, Lu NZ. A hot spot in the glucocorticoid receptor DNA-binding domain susceptible to loss of function mutation. Steroids 96 (2015) 115–120.

(4) Soon Cheon Shin, Anne Marie Singh, Kathleen Harris, Jihan Quraishi, Pedro Avila, and Robert P. Schleimer. The response of T cells and epithelial cells to staphylococcal enterotoxin B. Manuscript in preparation.

(5) Cho SH, Lee SH, Kato A, Takabayashi T, Kulka M, Shin SC, Schleimer RP. Cross-talk Between Human Mast Cells and Bronchial Epithelial Cells in PAI-Production via TGF-β1. American Journal of Respiratory Cell and Molecular Biology, Am J Respir Cell Mol Biol. 2015 Jan; 52(1):88-95.

(6) Yun Cao, Ingrid Bender, Athanasios Konstantinidis, Soon Cheon Shin, Christine Jewell, John Cidlowski, Robert P. Schleimer, Nick Lu. Glucocorticoid receptor Translational isoforms underlie maturational stage-specific Glucocorticoid sensitivities of dendritic cells in mice and human. Blood (2013) 121:1553-1562

(7) Isaac Wu, Soon Cheon Shin, Yun Cao, Ingrid K. Bender, Nadereh Jafari, Gilbert Feng, Simon Lin, John A. Cidlowski, Robert P. Schleimer, Nick Z. Lu. Selective glucocorticoid receptor translational isoforms Reveal Glucocorticoid-induced apoptotic transcriptomes. Cell Death and Disease. (2013) Jan 10; 4:e453.

(8) Leslie Grammer, Soon C. Shin, Quynh-Ai Truon-Tran and Robert Schleimer. Impaired TLR signaling in mononuclear cells from patients with food allergy. Submitted JACI, (2009)

(9) J. Sotiriadis. S. C. Shin, D. Yim, D. Siever, and Y. B. Kim. Thomsen- Friedenreich Antigen Expression Increases Sensitivity of NK Cell Lysis of Cancer Cells. International Journal of Cancer.111, 388-397, (2004)

(10) Daesong Yim., J. Sotiriadis., K.S. Kim., S.C. Shin., H.B. Jie., M.F. Rothschild., Y. B. Kim. Molecular cloning, expression pattern and chromosomal mapping of pig CD69. Immunogenetics. 54:176~281 (2002)

(11) S.C.Shin. Application and Markets of Diagnostic Reagents. Bioindustry Journal. 20.28~43(1998)

(12) Kim J.W., Lee U.S., Lee K.S., Park M.K., S.C.Shin., Kim H.S. Detection of PT and APTT by using of Coagrex 700 Auto Blood Clotting Analyzer and Thrombocheck. Journal of Clinical Pathology and Quality Control. 20(1) 21~30

(1998)

(13) Kim C.Y., S.C.Shin., Lee S.H., Kim W. B. and Kim B.M. Expression and Purification of Human Immunodeficiency Virus p24, gp41 antigens in Escherichia Coli. The Journal of Korean Society of Virology. 28(1) 21~30

(1998)

(14) Kim C.Y., Kim B.M., Lee M.Y., S.C.Shin., Lee T.Y., Lee J.H., Kim W.B. Expression and Purification of Human Immunodeficiency Virus-1 p24, gp41 antigens in Escherichia Coli.'97 Spring, The meeting of the Korean Society of Industrial Microbiology. Apr. 212(1997)

(15) Kim G.W., Hong S.W., S.C.Shin., Lee S.H. and Kim W.B. Studies on the development of Enzyme Linked Immunosorbent Assay (ELISA) for hepatitis B surface antigen(HBsAg) by Monoclonal antibodies of different affinity constants. Arch. Pharm. Res. 10(10) 18 ~ 24 (1997)

(16) S.C.Shin., Park J.H., Kim G.T., Lee J.H., Kim C.Y., Lee M.Y., Kim B.M., Kim W.B. Expression and purification of Korean Type Hepatitis C Viral Core, Nonstructural protein NS3 and NS4 in Escherichia Coli. The Journal of Korean Society of Virology.25 (2) 119 ~ 127 (1995)

(17) Kim B.M., S.C.Shin. Simultaneous detection of HIV and HCV antibodies. The Journal of Korean Society of Blood and Blood Transfusion.1 (1)91~92(1994)

(18) S.C.Shin. Studies on the Dong-A's AIDS diagnostic kits. The journal of Transfusion. 4(1) 133 (1993)

B. Abstract

(1) Banuelos J, Shin SC, Lu NZ. Distinct glucocorticoid sensitivity of the Th17 cytokines in murine T hybridomas and primary cells. American Association of Immunologists Annual Meeting, 2015.

(2) Banuelos J, Shin SC, Lu NZ. A hot spot in the glucocorticoid receptor DNA-binding domain susceptible to loss of function mutation, 16th International Congress of Endocrinology & the Endocrine Society’s 96th Annual Meeting & Expo, 2014.

(3) S. H. Cho, Soon C. Shin, A. Kato, R. P. Schleimer, Enhanced Production of Plasminogen Activator Inhibitor-1 (PAI-1) from Bronchial Epithelial Cells by Activated Human Mast Cells (MC). AAAAI 2009, Mar. 13~17, Washington D.C., USA (2009)

(4) N. Wang, MC Nlend, K. Thavarajah, A. Kato, SC Shin, S. Favoreto, A. Nair, RP Schleimer, JI Sznjder, and PHS Sporn. Hypercapnia inhibits Lipopolysaccharide- induced TNF and IL-6 mRNA and Protein Expression in differentiated THP-1 Macrophages. American Thoracic Society 2007 International Conference, May 18~23, Sanfrancisco, USA (2007)

(5) D. A. Wagner, S. C. Shin, B. P. Tancony, R. P. Schleimer, and L. C. Grammer. Activation of epithelial cells and lymphocytes by staphylococcal enterotoxin B induces profound expression of inflammatory cytokines and chemokines. AAAAI 2006, Mar. 3~7, Miami Beach, USA. (2006)

(6) J. I. Tirumalasetti, S. C. Shin, A. C. Miranowski, Q. A. Truong-Tran, L. C. Grammer, P. A. Greenberger, R. P. Schleimer. The TLR7 ligand loxoribine induces pentraxin 3 expression in peripheral blood mononuclear cells. AAAAI 2006, Mar. 3~7, Miami Beach, USA. (2006)

(7) S. C. Shin, Y-S. Kim, J. W. Yoon, and Y. Kim. Development of DTH and CTL in the SPF miniature swine immunized with the T/Tn antigen vaccine. ICI 2004. Jul. 17~23, Montreal, Canada, (2004)

(8) S. C. Shin, P. Kamien, A. Satyadev, J. Sotiriadis, Y-S. Kim, J-W. Yoon, and Y. Kim. T/Tn antigen expression and susceptibility of human carcinoma cells to direct killing by porcine natural killer cells and porcine immature dendritic cells. AAI Immunology 2004. Apr.17~23, Washington DC, (2004).

(9) Y-S. Kim, S.C. Shin, and Y.B.Kim. Effects of Microbial Flora on the Diversification of Porcine Ig Genes. 32nd Annual Autumn Immunology Conference. Nov. 22~24. Chicago.(2003)

(10) S.C. Shin, P. Kamien, A. Satyadev,Y-S. Kim, J-W. Yoon, and Y.B.Kim. T/Tn Antigen Expression and Susceptibility of Human Carcinoma Cells to Direct Killing by Porcine Natural Killer Cells and Porcine Immature Dendritic Cells. 32nd Annual Autumn Immunology Conference. Nov. 22~24. Chicago, (2003)

(11) Y-S. Kim, S.C. Shin, and Y.B.Kim. Effects of Microbial Flora on the Diversification of Porcine Ig Genes. AAI Immunology 2003. May 6~10, Denver, Colorado, (2003)

(12) S.C. Shin, P. Kamien, M. Choi, J. Sotiriadis, Y. Kim, and Y. B. Kim. T/Tn Antigen Expression and Susceptibility of Human Carcinoma Cells to Direct Killing by Porcine Natural Killer Cells and Porcine Immature Dendritic Cells. AAI Immunology 2003. May 6~10, Denver, Colorado, (2003)

(13) D. Yim, H-B. Jie, J. Sotiriadis, S.C. Shin, and Y. B. Kim. Molecular cloning and expression pattern of porcine CD16. Experimental Biology 2002, 927.13. Apr. 20 ~ Apr. 24. New Orleans, LA, (2002)

(14) H-B. Jie, D. Yim, S.C. Shin and Y. B. Kim. Molecular characterization of porcine CD16 complex. Experimental Biology 2002. 927.12. Apr. 20 ~ Apr. 24. New Orleans. LA, (2002)

(15) J. Sotiriadis, S.C.Shin, D. Yim, D. Sieber and Y. Kim. Human glycoprotein carcinoma antigens that active xenogeneic NK cells. Experimental Biology 2002, 507.14. Apr. 20 ~ Apr. 24. New Orleans. LA. (2002)

(16) Daesong Yim, Hyun-Bae Jie, John Sotiriadis, S.C. Shin, and Yoon Berm Kim. Molecular Cloning and Expression pattern of Porcine NKG2D, MDL-1 and CD69. Autumn Imminolgy Conference. AIC-(2001), Marriot Hotel. Chicago, IL60611. Nov. 17~19. Vol (30). No (146). P35. (2001)

(17) John Sotiriadis, S.C. Shin, Daesong Yim, and Yoon Berm Kim. Glycoprotein Antigens That May Potentially Activate Xenogeneic NK Cells. Autumn Immunology Conference. AIC-2001. Marriot Hotel. Chicago, IL60611. Nov. 17~19. Vol (30). No (146). P35. (2001)

(18) Hyun-Bae Jie, Daesong Yim, S.C. Shin and Yoon Berm Kim. Molecular Characterization of Porcine CD16 complex. Autumn Immunology Conference. AIC-2001. Marriot Hotel, Chicago. IL60611. Nov. 17~19. Vol (30). No.146. P50. (2001)

(19) J. Sotiriadis, S.C. Shin, D. Yim, and Y.B. Kim. Glycoprotein Antigens That May Potentially Activate Xenogeneic NK Cells. Experimental Biology 2001. 949.9. Mar. 31 ~ Apr. 4. Orlando, FL. (2001)

(20) Y-S. Kim, D. Yim, H-B. Jie, J. Sotiriadis, S.C. Shin, M.A. Fennawald and Y.B. Kim. The Diversification of the Antibody Repertoire in Germfree, Specific Pathogen-free and Conventionalized Piglets. Experimental Biology 2001. 781.1

. Mar. 31 ~ Apr. 4. Orlando, FL. (2001)

(21) D. Yim, H-B. Jie, J. Sotiriadis, Y-S. Kim, S.C. Shin, L.L. Lanier and Y.B. Kim. Expression of NKG2D and MDL-1 on Porcine Myeloid Cells. Experimental Biology 2001. 537.9. Mar. 31 ~ Apr. 4. Orlando, FL. (2001)

(22) H-B. Jie, D. Yim, Y-S. Kim, S.C. Shin, and Y.B. Kim. Porcine NK cell cytotoxicity enhancing molecule (PNK-E) associated with CD16 molecule complex. Experimental Biology 2001, 530.4. Mar. 31 ~ Apr. 4. Orlando, FL.

(2001)

(23) Kim C.Y., Kim B.M., Lee M.Y., S.C.Shin, Lee T.Y., Kim W.B. Expression and Purification of Human Immunodeficiency Virus 1 p24, gp41 antigen in Escherichia Coli. International Symposium and 1997 Spring Meeting of the Korean Society of Microbiology. (1997)

(24) Kim C.Y., S.C.Shin, et al. Development of HIV-1 ELISA using highly reactive HIV-1 Antigens. The Korean Society of Transfusion. (1997)

(25) S.C.Shin, Kim W.B. and Yang J.I. A novel Enzyme-Linked Immunosorbent Assay for the Simultaneous Detection of antibodies to Human Immunodeficiency Virus type 1 and Hepatitis C Virus. 4th Pacific Rim Biotechnology Conference. Feb. (1995)

(26) S.C.Shin A novel Immunosorbent Assay (ELISA) for the simultaneous detection of antibodies to Human Immunodeficiency Virus type 1 (HIV-1) and Hepatitis C Virus (HCV). 4th Western Pacific Congress of Chemotherapy and Infectious Disease . Dec. Australia. (1994)

(27) Lee T.Y., Lee Y.L., S.C.Shin, and Yang J.M. cDNA cloning and sequence determination of the hypervariable region of hepatitis C virus isolated from Korean patients. The Korean Molecular Biology. (1994)

(28) Kim B.M., S.C.Shin, Kim W.B. Growth of CHO-K1 cells in serum-free medium. The 48th Korean Society of Biological Science. 221(1993)

(29) S.C.Shin. Park J.H., Na G.H., Kim K.T., Lee J.H. Lee M.Y., Kim W.B. The important features in clinical diagnosis of Korean Type Hepatitis C Virus non- structural antigen p27. The Korean Society of Microbiology. 198 (1993)

(30) Park J.H., S.C.Shin, Kim K.T., Lee J.H., Na K.H., Lee M.Y., Kim W.B. Expression of Korean hepatitis C virus capsid protein in Escherichia coli and it's purification. The Korean Society of Microbiology. 198 (1993)

(31) S.C.Shin, Park J.H., Lee J.H., Na K.H., Kim K. T., and Kim W.B. Expression of Korean hepatitis C virus non-structural proteins in Escherichia coli and their purification. The Korean Society of Microbiology. 187 (1993)

(32) S.C.Shin. Studies on the Dong-A's AIDS Diagnostic Kits. The Korean Society of Transfusion. (1993)

(33) S.C.Shin, Kim W.B. and Chung T.J. Flow cytometry Analysis of Epstein-Barr Virus transformed Human B-lymphocytes. International Congress of New Drug Development. Seoul. (1991)

(34) S.C.Shin, Kim W.B. and Chung T.J. Production of LT-like factor by Epstein- Barr Virus transformed Human B-lymphocytes. International Congress of New Drug Development. Seoul. (1991)

PATENT

(1) S.C.Shin, Lee J. H. The simultaneous detection methods of anti HCV, anti HIV-1, anti HIV-2 & HTLV-1. Korean Patent Regulated Number 1371173 (1998.2.4)

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