Assistant Research

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Anuradha Rajulapati Allen, TX ***13

*************@*****.*** • 304-***-****

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Qualifications Profile

Analytical and skilled Research Associate with solid problem solving, research, and analysis skills, as well as a Master of Science degree in Chemistry.

Adept at assisting with and conducting laboratory experiments on research projects within well-defined guidelines.

Proven ability to maintain efficient operation of a laboratory, including providing supervision and guidance to junior level technicians.

Keeps abreast of current publications relative to methods, techniques, and developments within area of research; collects, prepares, and analyzes specimens.

Skilled at documenting work in a timely manner, assist in writing of research documents, and presenting data to audiences.

Educational Background

Master of Science in Chemistry, 2009

MARSHALL UNIVERSITY – Huntington, WV

Thesis: High Resolution Single Molecule Optical Localization of Multiple Fluorophores on DNA Origami Constructs; 3.50 G.P.A.

Bachelor of Science in Pharmacy, 2006

SRI PADMAVATHI WOMEN’S UNIVERSITY – Tirupati, Andhra, Pradesh, India 3.67 G.P.A.

Professional Experience

UT HEALTH NORTH EAST, Tyler, TX 11/2014 to 7/2017

Research Associate

Investigated the pathophysiological significance of factor VIIa interaction with endothelial cell protein C receptor.

Analyzed the mechanisms by which tissue factor and EPCR contribute or influence pathogenesis of various diseases, including cancer and inflammation.

Provided overall lab management, leading the designated research project.

Maintain and upkeep of transgenic mouse colony.

Utilized various techniques, including mouse breeding and colony management, Tail vein injections, Cell culture, Immunohistochemistry, ELISA, western blotting, Protein purification by affinity chromatography,antibody purifications by column chromatography, PCR and RT-PCR. Anuradha Rajulapati Page 2

UNT HEALTH SCIENCE CENTER, Fort Worth, TX 3/2012 to 10/2014 Research Assistant

Worked on a research project focused on autonomic regulation of blood pressure under chronic intermittent hypoxia condition, stimulating obstructive sleep apnea.

Research techniques include microinjections, telemetry, perfusions, brain sectioning, immunohistochemistry, PCR, RT-PCR, cell culture, western blotting, etc.

Trained new graduate and undergraduate students. MBR CANCER CENTER, WEST VIRGINIA UNIVERSITY, Morgantown, WV 5/2010 to 2/2012 Biological Technician

Studied the role of HEF1 and AurA in breast cancer and role of HDAC6 and Cortactin in invasion and metastasis of breast cancer.

General laboratory management, including training new lab members, ordering supplies and maintaining the lab environment.

Utilized Cell culture, Cell transfection, Viral infection and creation of stable cell lines, Immunofluorescence, bacterial culture, Plasmid DNA isolation, molecular cloning, DNA and RNA extraction and purification, Kinase Assay, ELISA, Western blotting, PCR, Gelatin/Casein Zymography techniques in assigned research projects..

Maintain and upkeep of transgenic mouse colony.

MARSHALL UNIVERSITY, Huntington, WV 1/2008 to 5/2010 Research Lab Technician, Department of Biology, 3/2010 to 5/2010

Analyzed the toxicity of Ceriumoxide nanoparticles in C.elegans.

Troubleshot various immunohistochemistry protocols on C.elegans.

Conducted general laboratory management, Protein-gel analysis and western blotting, plasmid isolation, cell culture work.

Made reagents and solution as necessary for the laboratory. Graduate Research Assistant, Department of Chemistry, 1/2008 to 3/2010

Completed Master’s thesis, High Resolution Single Molecule Optical Localization of Multiple Fluorophores on DNA Origami Constructs.

Thesis research, laboratory record keeping, tracking of project resources, preparation of stock solutions, and autoclaving tips and containers.

Involved in overseeing laboratory safety and compliance.

Synthesis and purification of fluorescently labeled Dendrimer-DNA complexes and DNA origami, Imaging DNA origami sample by Optosplit microscope (Two channel fluorescence microscope), analyzing results by MATLAB and Lab VIEW software.

Analyze samples using mass spectrometry.

Anuradha Rajulapati Page 3

Scientific Publications

Gaddam SK, Sundaram J, Rajulapati A, Pendurthi UR, Lella VMR. Factor VIIa interaction with EPCR modulates the hemostatic effect of rFVIIa in hemophelia therapy:mode of its action.Journal of Blood Advances 2017 June 1:1206-1214. DOI : 10.1182/BLOODADVANCES 201-***-****. Gaddam SK, Sundaram J, Rajulapati A, Pendurthi UR, Lella VMR. Pharmacological Concentrations of recombinant factor VIIa restores hemostasis independent of tissue factor in antibody-induced Haemophelia mice. Journal of Thrombosis and Haemostasis 2016 Feb 14(3):546-50. PMID:26727350. Kozyulina PY, Loskutov YV, Kozyreva VK, Rajulapati A, Ice RJ and Pugacheva EN. Pro-metastatic protein NEDD9 regulates individual cell migration via caveolin-1 dependent trafficking of integrins. Mol Cancer Res. 2015 Mar 15; 13(3):423-38. PMID: 25319010.

Kozyreva VK, McLaughlin SL, Livengood RH, Calkins RA, Kelley LC, Rajulapati A, Ice RJ, Smolkin MB, Weed SA, Pugacheva EN. NEDD9 regulates actin dynamics through cortactin deacetylation in an AURKA/HDAC6-dependent manner. Mol Cancer Res. 2014 May 26; 12(5):681-93. PMID: 24574519. McLaughlin SL, Ice RJ, Rajulapati A, Kozyulina PY, Livengood RH, Kozyreva VK, Loskutov YV, Culp MV, Weed SA, Ivanov AV, Pugacheva EN. NEDD9 depletion leads to MMP14 inactivation by TIMP2 and prevents invasion and metastasis. Mol Cancer Res. 2014 Jan 7; 12(1):69-81. PMID: 24202705. Bathina CS, Rajulapati A, Franzke M and Mifflin SW. Knockdown of tyrosine hydroxylase in the nucleus of the solitary tract reduces elevated blood pressure during chronic intermittent hypoxia. Am J Physiol Regul Integr Comp Physiol. 2013 Sep 18. PMID: 24049117. Oral Presentations

Bathina CS, Rajulapati A, Franzke M, Yamamoto K and Mifflin SW. Role of Catecholaminergic A2 neurons of nucleus of the solitary tract (NTS) in cardiovascular and respiratory adaptations to chronic intermittent hypoxia (CIH) in rats. Featured Topic: Neural Mechanisms In Cardiovascular Regulation, FASEB 2013, Gleneden Beach, OR.

Bathina CS, Rajulapati A, Franzke M, Kenta Yamamoto, Tom Cunningham and Mifflin SW. Knockdown of tyrosine hydroxylase in the nucleus of the solitary tract reduces elevated blood pressure during chronic intermittent hypoxia. Invited talk; Featured Topic: Central Nervous System Modulation of Cardiorespiratory Responses to Hypoxia, EB 2013, Boston, MA. Gaddam SK, Sundaram J, Rajulapati A, Penduruthi UR and Lella VM. Pharmacological Concentrations of Recombinant Factor VIIa Restores Hemostasis in Acquired Hemophilia Primarily Independent of Tissue Factor. Submitted to 56th Annual meeting and exposition 2015 of American Society of Hematology, Orlando, FL.

Presentations & Abstracts

Gaddam SK, Sundaram J, Rajulapati A, Pendurthi UR, Lella VMR. Pharmacological Concentrations of FVIIa restores hemostasis independent of tissue factor in antibody-induced Haemophelia mice. Journal of Blood 2015 Nov 126:293.

Anuradha Rajulapati Page 4

Gaddam SK, Sundaram J, Rajulapati A, Penduruthi UR and Lella VM. Pharmacological Concentrations of Recombinant Factor VIIa Restores Hemostasis in Acquired Hemophilia Primarily Independent of Tissue Factor. Submitted to 56th Annual meeting and exposition 2015 of American Society of Hematology, Orlando, FL from December 5-8th 2015.

Elena N. Pugacheva, Sarah mClAUGHLIN, Ryan Ice, Rajulapati A, Polina Kozyulina, Ryan Livengood, Varvara Kozyreva, Yuriy Loskutov, Alexey Ivanov, and Scott Weed. NEDD9 depletion leads to MMP14 inactivation by TIMP2 and prevents invasion and metastasis. AACR Annual Meeting 2014; April 5-9, 2014 San Diego, CA.

Bathina CS, Rajulapati A, Nagalla V, Nedungadi TP and Mifflin SW. Angiotensin receptor subtype AT1a and AMPA receptor subunit GluR2 mRNA levels are reduced in A2 neurons of nucleus of the solitary tract following chronic intermittent hypoxia. Experimental Biology 2013, Boston, MA. Bathina CS, Rajulapati A, Franzke M, Yamamoto K and Mifflin SW. Role of Catecholaminergic A2 neurons of nucleus of the solitary tract (NTS) in cardiovascular and respiratory adaptations to chronic intermittent hypoxia (CIH) in rats. Neural Mechanisms In Cardiovascular Regulation, FASEB 2013, Gleneden Beach, OR.

Bathina CS, Rajulapati A, Franzke M and Mifflin SW. Knockdown of tyrosine hydroxylase in the nucleus of the solitary tract reduces elevated blood pressure during chronic intermittent hypoxia. Experimental Biology 2013, Boston, MA.

Yamamoto K, Bathina CS, Rajulapati A, Franzke M and Mifflin SW. Acute intermittent optogenetic stimulation of glutamatergic NTS neurons induces sympathetic long-term facilitation. Experimental Biology 2013, Boston, MA.

Bathina CS, Rajulapati A, Franzke M and Mifflin SW. Knockdown of tyrosine hydroxylase in the nucleus of the solitary tract reduces elevated blood pressure during chronic intermittent hypoxia. High Blood Pressure Council Research, Washington DC, 2012.

Two Dimentional Materials as Substrates for the Development of Origami Based Bio-nano-sensors. Rahman Masudur, Hong Zhong, Anuradha Rajulapati, Dawn Nicholas, Anne Howells, Nathaniel Crow, Neff David, Michael Norton. (Publication in NANO-DDS 2009) Development of Systems and Methods for the Sub-Pixel Resolution Characterization of Nanoscale Constructs Containing Multiple Fluorophores. Anuradha Rajulapati, David Neff, Michael Norton

(Presented at Sigma Xi Research day April 2009, Marshall University).

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