Customer Service Professional Experience
Resume:
Rita O. Ebinum
Laurel, MD 20708
E-mail: ********@*****.***
Cell: 240-***-****
Education: Salisbury University, Salisbury MD
Bachelors of Science in Biology December 2010
Professional Experience
Company: Paragon Bioservices Inc
Title: Associate Scientist II July 2013-Present
Set up and Run 2L-200L Bioreactors
Scale up and maintain mammalian and insect cultures (small and large-scale production)
Work closely with production management and process development to initiate manufacturing projects and help develop processes/techniques
Scale up cells and run bioreactors in GMP
Proficient in the use of welders and sealers and different size tubing for bioreactor run
Transfection and infection of cells for protein expression
Excellent Aseptic technique for cell split, cell thaw, cell Feed and cell passaging
Freezing and harvesting of mammalian and insect cells
Working with suspension cell culture and adherent cell culture in
Proficient in the use of Nova Flex Bioprofile and Vi-cell
Perform Media prep, buffer prep and other Reagent for Cell feed and harvest
Monitoring and sampling of cultures in flasks and bioreactors
Maintain and scale up mammalian and insect cultures in suspension flask and CellStack
Set up and run Wave bioreactors
Run iCeLLis Nano bioreactors
Order and Stock lab supplies use in both insect and mammalian lab
Ship out completed material to clients
Assemble, Wash, and Autoclave equipment use for production
Write PFD and batch record for production use
Use centrifuge and Unifuge to harvest cells at the end of production
Perform Depth Filtration after cell harvest
Use Automatic Cell Counter and Microscope to Count inoculated bioreactors
Submit Samples for PCR, qPCR, ELISA, SDS-PAGE and Western Blot for testing
Company: Medimmune (Contract) February 2012-July 2013
Title: Manufacturing Technician
Operated and Ran 80L-17,000L bioreactors
Performed open and closed system Aseptic processing and Aseptic manipulation
Used welders and sealers for close system processes
Performed media prep, buffer prep and other Reagent preps for Cell feed and Cell harvest
Washed, autoclaved, assembled glassware and equipment used in GMP
Performed daily cell count using Vi-cell analyzer
Pulled samples from bioreactors for QC Testing daily
Bioreactor clean in place (CIP) and Steam in place (SIP)
Experience in a GMP manufacturing facility as both a performer and verifier for various processes requiring strict adherence to Master Batch Record
Used Osmometer, pH meter, Conductivity meter, Welder and Sealer during production
Flushed filters and tested the filters using IT4 Tester for integrity
Used Human Machine Interfac (HMI) during GMP Production
Manually cleaned equipment used for GMP production
Company: Human Genome Sciences (Contract) June 2011-December 2011
Title: Manufacturing Technician
Washed and autoclaved parts used in the GMP manufacturing
Clean in place (CIP) and Steam in place (SIP)
Performed daily tasks according to Standard Operating Procedure(SOP) and Good Laboratory Practice
Company: Howard Community College April 2008-April 2009
Title: Lab Technician
Worked and Set up lab for both inorganic and organic chemistry lab containing 80 students
Ordered and stocked lab supplies used in both inorganic and organic chemistry lab
Performed variety of procedures such as titration and calculation to make up solution used in the lab
Stocked chemicals for students to use for different lab experiments
Cleaned up and arranged lab daily
Worked with lab instructors and lab managers to maintain a high quality lab environment
Company: Lowes Inc. June 2006-June 2011
Cashier/Customer service
Provided fast- past and friendly customer service
Answered phones
Operated cash register
Processed customer payments
Copied and faxed forms
Worked in team to solve customer problems
Excellent customer service skills
Instruments
Bioreactor
Nova Flex Bioprofile
Centrifuge
Unifuge
Hemocytometer
Automatic Cell Counter
Sterile BioWelder
Sealer
Vi-CELL Cell Viability Analyzer
Countess
Balances, Pipettes
Microscopes
Incubator
Water bath
Osmometer
Conductivity Meter
Scales
Biosafety Cabinet
pH meter
Incubator
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