Rita O. Ebinum

***** *** ***** ***** ****

Laurel, MD 20708

E-mail: ac3x2f@r.postjobfree.com

Cell: 240-***-****

Education: Salisbury University, Salisbury MD

Bachelors of Science in Biology December 2010

Professional Experience

Company: Paragon Bioservices Inc

Title: Associate Scientist II July 2013-Present

Set up and Run 2L-200L Bioreactors

Scale up and maintain mammalian and insect cultures (small and large-scale production)

Work closely with production management and process development to initiate manufacturing projects and help develop processes/techniques

Scale up cells and run bioreactors in GMP

Proficient in the use of welders and sealers and different size tubing for bioreactor run

Transfection and infection of cells for protein expression

Excellent Aseptic technique for cell split, cell thaw, cell Feed and cell passaging

Freezing and harvesting of mammalian and insect cells

Working with suspension cell culture and adherent cell culture in

Proficient in the use of Nova Flex Bioprofile and Vi-cell

Perform Media prep, buffer prep and other Reagent for Cell feed and harvest

Monitoring and sampling of cultures in flasks and bioreactors

Maintain and scale up mammalian and insect cultures in suspension flask and CellStack

Set up and run Wave bioreactors

Run iCeLLis Nano bioreactors

Order and Stock lab supplies use in both insect and mammalian lab

Ship out completed material to clients

Assemble, Wash, and Autoclave equipment use for production

Write PFD and batch record for production use

Use centrifuge and Unifuge to harvest cells at the end of production

Perform Depth Filtration after cell harvest

Use Automatic Cell Counter and Microscope to Count inoculated bioreactors

Submit Samples for PCR, qPCR, ELISA, SDS-PAGE and Western Blot for testing

Company: Medimmune (Contract) February 2012-July 2013

Title: Manufacturing Technician

Operated and Ran 80L-17,000L bioreactors

Performed open and closed system Aseptic processing and Aseptic manipulation

Used welders and sealers for close system processes

Performed media prep, buffer prep and other Reagent preps for Cell feed and Cell harvest

Washed, autoclaved, assembled glassware and equipment used in GMP

Performed daily cell count using Vi-cell analyzer

Pulled samples from bioreactors for QC Testing daily

Bioreactor clean in place (CIP) and Steam in place (SIP)

Experience in a GMP manufacturing facility as both a performer and verifier for various processes requiring strict adherence to Master Batch Record

Used Osmometer, pH meter, Conductivity meter, Welder and Sealer during production

Flushed filters and tested the filters using IT4 Tester for integrity

Used Human Machine Interfac (HMI) during GMP Production

Manually cleaned equipment used for GMP production

Company: Human Genome Sciences (Contract) June 2011-December 2011

Title: Manufacturing Technician

Washed and autoclaved parts used in the GMP manufacturing

Clean in place (CIP) and Steam in place (SIP)

Performed daily tasks according to Standard Operating Procedure(SOP) and Good Laboratory Practice

Company: Howard Community College April 2008-April 2009

Title: Lab Technician

Worked and Set up lab for both inorganic and organic chemistry lab containing 80 students

Ordered and stocked lab supplies used in both inorganic and organic chemistry lab

Performed variety of procedures such as titration and calculation to make up solution used in the lab

Stocked chemicals for students to use for different lab experiments

Cleaned up and arranged lab daily

Worked with lab instructors and lab managers to maintain a high quality lab environment

Company: Lowes Inc. June 2006-June 2011

Cashier/Customer service

Provided fast- past and friendly customer service

Answered phones

Operated cash register

Processed customer payments

Copied and faxed forms

Worked in team to solve customer problems

Excellent customer service skills

Instruments

Bioreactor

Nova Flex Bioprofile

Centrifuge

Unifuge

Hemocytometer

Automatic Cell Counter

Sterile BioWelder

Sealer

Vi-CELL Cell Viability Analyzer

Countess

Balances, Pipettes

Microscopes

Incubator

Water bath

Osmometer

Conductivity Meter

Scales

Biosafety Cabinet

pH meter

Incubator

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