Postdoctoral Research Associate

Joy Michelle Blain, Ph.D.

ac3wy5@r.postjobfree.com www.linkedin.com/in/joy-blain

**** *. ****** *** ****, AZ 85206

Summary of Qualification

Enthusiastic research biochemist with over 5 years of experience in analytical chemistry, medicinal chemistry, molecular biology, and biochemistry. Proficiency in molecular biology techniques, drug discovery, biomarker discovery, insect cell culture, and immunoassays. Works well independently, as well as part of a team. Excellent communication and teaching skills, multi-tasking, and deadline oriented.

Education

Ph,D., Biochemistry, Northern Illinois University, DeKalb, IL August 2016

Master of Science, Analytical Chemistry, Governors State University, University Park, IL May 2011

Bachelor of Science, Chemistry, University of Illinois Chicago, Chicago, IL May 2008

Research Experience

2016-PRESENT Postdoctoral Research Associate – Arizona State University

Identifying autoantibodies against glycoproteins for the early detection of Breast Cancer

High-throughput screening of glycosylated antigens using protein microarrays

Expression and purification of glycosyltransferases from Insect Cells

Development of ELISA validation assay for autoantibodies against glycoproteins

Development of a Luminex validation assay for autoantibodies against glycoproteins

2014-2016 RESEARCH ASSISTANT - Northern Illinois University

Studied synthesis and characterization of small molecule/enzyme interactions

•Synthesis of small molecules as potential anti-infective inhibitors

•Developed activity assays to characterize the inhibition of enzymes by small molecule inhibitors synthesized

•Developed experience in x-ray protein structure determination through crystal screening, collection of x-ray diffraction data, processing data and map fitting

•Developed proficiency in protein engineering strategies (molecular biology techniques, mutagenesis, phage display)

•Mentored three undergraduate students

2011-2014 TEACHING ASSISTANT - Northern Illinois University

Studied synthesis and characterization of small molecule/enzyme interactions

•Developed expertise in protein biophysical techniques including calorimetry, surface plasmon resonance (SPR), and circular dichroism (CD)

•Assessed the binding affinity of potential anti-infective small molecule inhibitors

•Developed proficiency in subcloning, expressing, and purifying recombinant proteins

•Mentored four undergraduate students

2009-2011 RESEARCH ASSISTANT - Governor’s State University

Studied the development and methods in the treatment of chronic wounds

•Developed expertise in preparing biocompatible nano-emulsions

•Examine the DNA photocleavage, cytotoxicity, phototoxicity, and singlet-oxygen formation of nano-emulsions

2004-2005 SUMMER INTERNSHIP - Medpointe Pharmaceuticals

•Quality Control - Physical Characterization of Tablets and Suspensions

Teaching Experience

2011-2014 TEACHING ASSISTANT - Department of Chemistry and Biochemistry, Northern Illinois University

•Analytical Chemistry I Laboratory

•General Chemistry II Recitation

•General Chemistry I Recitation

•General Chemistry II Laboratory

Professional Experience

2011 SHERWIN WILLIAMS - Associate Chemist

Formulator in the High Performance Urethane Group, working on Solventborne 2K Urethanes and Moisture Cure Urethanes

•Development of new products

•Developed expertise in long-term testing using QUV, Saltfog, Humidity, and Corrosive Weathering

•Safety Auditor

•5S Auditor

2008-2011 SHERWIN WILLIAMS - Associate Chemist

Formulator in the High Performance Waterborne Group, working on acrylics, epoxies, and alkyds

•Development of new products

•Developed expertise in long-term testing using QUV, Saltfog, Humidity, and Corrosive Weathering

•Capital Improvement Committee

•Technical Summit Committee

•Raw Material Strategy Initiative Committee

Peer Reviewed Publications

Blain, J.M. (2016) Characterization of potential anti-infective agents of Burkholderia pseudomallei targeting IspF. ProQuest Dissertations Publishing. ISBN: 978**********.

Goshu, G.M., Ghose, D., Bain, J.M., Pierce, P.G., Begley, D.W., Hewitt, S.N., Udell, H.S., Myler, P.J., Meganathan, R., Hagen, T.J., (2015), Synthesis and biological evaluation of pyrazolopyrimidines as potential antibacterial agents. Bioorganic & Medicinal Chemistry Letters doi: http://dx.doi.org/10.1016/j.bmcl. 2015.10.096

Zhang Z, Jakkaraju S, Blain J.M., Gogol K, Zhao L, et al. (2013) Cytidine derivatives as IspF inhibitors of Burkolderia pseudomallei. Bioorg Med Chem Lett 23: 6860–6863 doi:10.1016/j.bmcl.2013.09.101

Blain, J.M., Ghose, D., Gorman, J.L., Goshu, G.M., Raneiri, G., Zhao, L., Meganathan, R., Walter, R.L., Hagen, T.J., and Horn, J.R.. Synthesis and Characterization of the Burkholderia pseudomallei IspF Inhibitor L-tryptophan hydroxamate. (In Preparation)

Blain, J.M., Goshu, G., Raneiri, G., Walter, R.L., Hagen, T.J., and Horn, J.R.. Enzyme Engineering for the Development of a High Throughput Temperature Screen of Burkholderia pseudomallei IspF Inhibitors. (In Preparation)

Presentations

Enzyme Engineering for the Development of a High Throughput Thermal Shift Assay of Burkholdria pseudomallei IspF Inhibitors

Blain, J.M., Goshu, G.M., Ghose, D., Raneiri, G.M., Walter, R.L., Hagen, T.J., and Horn, J.R.

The 29th Annual Gibbs Conference on Biothermodynamics, October 3-6, 2015, Carbondale, IL

Photoactivated Riboflavin and Phylloquinone as Photodynamic Antimicrobial Chemotherapeutic Agents

Blain, J.M. and Fu, P.K.

42nd Central Region Meeting of the American Chemical Society, June 8-10, 2011, Indianapolis, IN

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