Biochemist Protein Purification Scientist

ASHUTOSH PUDASAINI

US Permanent Resident

**** ****** **., ******, ** 75206

940-***-**** *************@*****.***

EDUCATION

Doctor of Philosophy (Ph.D.), Chemistry and Biophysics, December 2016.

Southern Methodist University, Center for Drug Discovery, Design, and Delivery, Dallas, Texas.

Bachelor of Science, Biology, Summa Cum Laude, May 2011.

Texas Christian University, Fort Worth, Texas.

WORK EXPERIENCE

UT Southwestern Medical Center, Dallas, Texas

Postdoctoral Researcher: 11/2016-Present

•Studied the HIF2A interactome to identify new genes involved in hypoxia and cancer.

•Developed a method to study post-translational modifications of proteins associated with the SCFFBXL5 complex involved in iron homeostasis in mammals.

•Expressed and purified protein complexes under various metabolic states (iron/oxygen).

•Evaluated loss of function using RNAi methods to study effects of iron-stress in mammalian cells.

•Expressed and purified proteins in E. coli, insect, yeast and mammalian cells.

•Proficient in biochemistry, cell and molecular biology techniques.

Southern Methodist University, Dallas, Texas.

Research Assistant: 06/2012-10/2016.

•Gained expertise in techniques such as SDS-PAGE, western blot, ELISA, SEC, MALS, UV/VIS Spectroscopy, Kinetics, DLS, DSC, and X-Ray Crystallography.

•Developed the automated protein purification protocols on the GE AKTA system.

•Discovered a the signaling mechanisms of ZEITLUPE, a circadian clock protein.

•Developed an optogenetic tool to evaluate protein folding and stability in vivo.

•Developed a mathematical model to simulate protein degradation and the overall effects of blue light in plant circadian rhythm.

•Expressed and purified proteins in E. coli, yeast and mammalian cells.

•Solved the X-Ray structures of 5 different protein variants.

•Supervised and trained more than 20 students and other laboratory staff.

Southern Methodist University, Dallas, Texas.

Research Technician: 11/2011-06/2012.

•Purified milligram to gram scale of proteins expressed in E coli and yeast.

•Performed protein characterization by UV/VIS Spectrometry, DSC, and ATKA-FPLC.

•Collected data for the procurement of grants (2).

•Provided direct support for the establishment of lab protocols, safety guidelines, and training modules.

SPECIFIC SKILLS

Laboratory: Cell culture, protein expression (E coli, yeast, insect and mammalian cells), isotopic labeling, protein purification, ATKA systems, molecular cloning, SEC/FPLC, HPLC, MALS, DLS, PCR, RT-PCR, qPCR, X-Ray crystallography, NMR, DSC, peroxide assay, photochemistry, enzyme kinetics, thermal shift assay, pulldown assay, protein-protein interaction, western blot, drug screening, and assay design.

Technical: Coot, Phenix, CCP4, MATLAB, Microsoft Office Suite, GraphPad Prism, OriginLab, Photoshop, and computing/programming (HTML, CSS, Linux OS, SAS, and R).

PUBLICATIONS

•Pudasaini, A., Shim, J.S., Song, Y.H., Shi, H., Kiba, T., Somers, D.E., Imaizumi, T., Zoltowski, B.D. (2017) “Kinetics of the LOV domain of ZEITLUPE determine its circadian function in Arabidopsis.” eLife 2017;6:e21646.

•Pudasaini, A., El-Arab, K.K, Zoltowski, B.D (2015) “LOV-based optogenetic devices: light-driven modules to impart photoregulated control of cellular signaling.” Front Mol Biosci. 2015; 2: 18.

•El-Arab, K.K, Pudasaini, A., Zoltowski, B.D (2015) “Short LOV proteins in Methylocystis reveal insight into LOV domain photocycle mechanisms.” PLOS One 10(5): e0124874.

•Pudasaini, A., Zoltowski, B.D. (2013) “Zeitlupe senses blue-light fluence to mediate circadian timing in Arabidopsis thaliana.” Biochemistry, 52 (40), pp 7150–7158.

SELECTED PRESENTATIONS

•Photosensory Receptors & Signal Transduction, Gordon Research Seminars (GRS), Jan 24 2016, Galveston Texas, USA (Oral).

•Photosensory Receptors & Signal Transduction, Gordon Research Conference (GRC), Jan 27-28 2016, Galveston Texas, USA, #13 (Poster).

•International Symposium on Plant Photobiology, May 26-31 2015, Austin Texas, USA (Poster)

•Biochemistry Cell Signaling and Drug Discovery, 70th ACS Southwest Regional Meeting, 19 November 2014, Fort Worth Texas, SWRM-575 (Oral).

•Photosensory Receptors & Signal Transduction, Gordon Research Conference (GRC), 6-11 April 2014, Lucca, Italy, P35 (Poster).

SELECTED HONORS AND AWARDS

•Beihl Graduate Fellowship (2015-16, Southern Methodist University).

•Lazenby Graduate Fellowship (2012-2016, Southern Methodist University).

•Dean’s Departmental Award (2014, Southern Methodist University).

•2nd prize for Oral Presentation (2013, UT Tyler, ACS MiM).

•Summa Cum Laude (2011, Texas Christian University).

•Chancellor’s Full Tuition Scholarship (2009-2011, Texas Christian University).

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