Data Quality Assurance

Philip Stoeber

***** **** **** ************, **

301-***-****, *************@*****.***

Objective

A data scientist or bioinformatic developer position in a stimulating, cutting-edge and fast paced environment that allows me to effectively utilize my critical thinking, programming and analytical skills.

Education

University of Maryland University College College Park, MD MS, Bioinformatics December 2017

University of Maryland College Park College Park, MD

BS, Cell Biology and Molecular Genetics December 2014

Notable Projects

Linking Gene Expression to Cancer Mutations Capstone Project

Contributed to the development of version 3.0 of the Annotation, Visualization and Impact Analysis (AVIA) web application. AVIA presents data in an integrated view of allele frequencies isolated from specific cancer types within The Cancer Genome Atlas (TCGA) and offers a comparison with normal tissue expression data from the Genotype-Tissue Expression (GTEx) Project. This project utilized Perl, MySQL, AWS, HTML, CSS, JavaScript.

Bioinformatic Gene Prediction Algorithm

Utilized Perl to develop a gene prediction algorithm that takes a single stranded DNA input sequence and predicts the open reading frames (ORFs) within the forward and reverse sequence. ORFs are parsed out of the input string based on Codon matching using regular expression. The resulting output was then used for Blast queries.

Data Science/Bioinformatic File Parsing to MySQL Application

Utilizing Perl, AWS and MySQL, developed a script that queries a database stored on AWS for a specific list of IDs’. These ID’s are used to extract specific columns of data out of large data sets and generates file that is loaded into a new MySQL table created within the script.

Technical Skills

Programming Languages: Perl, Java, AWS, MySQL, PL/SQL, Oracle, HTML, CSS, Linux, R, knowledge of JavaScript, Tecan Robotics, Caliper Robotics, Hamilton Robotics

Frameworks: BioJava, BioPerl, GALAXY

Bioinformatic Tools: NCBI, EMBL-EBI, Ensembl, UCSC Genome Browser, BLAST, ClustalX, MUSCLE

Bioinformatic Databases: GenBank, TCGA, ICGC, GTEx, cBioPortal

Certifications

Tecan Robotics Durham, NC

Fluent Control Certification October 2017

Certified to develop methods and scripts for Tecan Fluent robotics for clinical laboratory assays

Ability to configure and maintenance Tecan Fluent instrument

Work History

GeneDx Gaithersburg, MD

Next Generation Sequencing Technologist II June 2016 – Present

Developed robotic applications enabling laboratory automation of clinical assays and assisted in the implementation of these programs

Developed standard operating procedures, training protocols and maintenance procedures for Tecan Fluent robot

Validated robotic programs for clinical assays

Mastery of Illumina NovaSeq 6000, Hi-Seq 3000/4000, Hi-Seq 2500 and MiSeq Next-Generation sequencing instruments

Able to prepare Next-Generation sequencing assays and perform quality assurance analysis

Experience with Exome Sequencing

Microarray Technologist I May 2015 – June 2016

Prepared qPCR clinical assays using Qiagen robotic instruments

Experience analyzing qPCR clinical assays and performing quality assurance checks

Health and Human Services Rockville, MD

Student Trainee Data Management July 2014 – August 2014

Contributor to collaborative teams responsible for solving clients' web development issues and data management problems

Developed data storage solutions through creation of new data libraries and lists

Consulted on OIT's operational processes and developed Microsoft SharePoint applications to capture, store and maintain these re-designed processes

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