NASIM ESKANDARI

Toronto, ON, Canada Phone Number: 647-***-**** Email: ac3mhq@r.postjobfree.com

Professional Profile Summary

Results-driven and self-motivated professional with over 6 years of progressive experience in biomedical research, protocol development and manuscript writing

Areas of expertise: cellular and molecular biology, biopreservation (monolayer and cell suspension cryopreservation), and cancer biology, microbiology

Understanding of current good manufacturing practice (cGMP), Good Laboratory Practice (GLP), and Health Canada Food and Drug Act, and Natural Health Product

Understanding of laboratory safety practices and WHMIS

Technical Skills

Microbiology: Sterility test, disinfectant efficacy, antibiotic-microbial assays, microbial limits test (MLT), and growth promotion test for raw material, in process & finished products

Cell biology: Cell culture and sub culturing of human primary cell and cell line, Cell counting, cell suspension and monolayer cryopreservation, plasmid transfection to mammalian cells by lipofectamine and electroporation, Phase contrast microscopy, fluorescent microscopy

Molecular biology: DNA and RNA extraction/ purification/ quantification / PCR,RT-PCR, plasmid extraction, gel electrophoresis

Assays: Cell membrane integrity assay, angiogenesis assay, Immunocytochemistry (ICC), MTT assay, wound healing Assay (scratch test), cAMP Assay

Other Relevant Skills

Multi-tasks, self-motivated, work well independently and in a team

Proven organizational and time management skills, as demonstrated through previous working experience

Excellent communication skills (verbal, written, presenter) both in one-on-one and in a group setting

Proficient in use of Microsoft office (Microsoft Word, Excel, PowerPoint), email, PubMed database, and SPSS statistical software

PROFESSIONAL EXPERIENCE

University of Alberta, Edmonton, AB Jan. 2015- Aug. 2017

Research Assistant

Project: Cryopreservation of human umbilical vein endothelial cells (HUVECs) in monolayer

Developed a protocol for cryopreservation of HUVECs in monolayers with high post-thaw absolute viability >95%

Investigated the effect of cooling rate, cryoprotectant concentration and composition on cryopreservation of HUVECs in monolayers

Identified microorganisms using a variety of methods in culture medium such as sterility testing (membrane filtration, direct inoculation)

Practiced aseptic cell culture techniques (cell culture with no antibiotic)

Conducted literature review, data collection and interpretation, reported results to supervisor

Calibrated laboratory instruments in lab level such as pH meter, analytical balance, weighing balance and micropipette for accurate result

Performed weekly laboratory maintenance, safety inspection reports, ordered reagents, updated and archived Safety Work Procedures (SWPs), MSDS and protocols

Neuroscience Research Center, Tehran, Iran Jan. 2013-June. 2014

Microbiologist

Prepared bacterial growth media (Broth and Agar), bacterial cell transformation with plasmid constructs

Perform Microbiological, chemical testing aseptically for ingredients, raw and processed products

Conducted antibiotic resistance test to select the transformed bacteria with plasmids

Performed media preparation, identification of microorganisms using a variety of methods in culture medium such as sterility testing (membrane filtration, direct inoculation)

Experienced cell culture, DNA, RNA extraction/quantification, RT-PCR (cDNA synthesis), cell transfection by lipofectamine, apoptosis assay

Evaluated, interpreted, and reported test results on a timely manner

Assisted in teaching and organizing practical session in the third school of International Brain Research Organization (IBRO)

Kalle (food manufacturing company), Tehran, Iran Dec. 2011-Dec. 2012

Laboratory Analyst/quality assurance

Pickup, log in and distribute test samples

Prepared reagents, standards, controls and sample for analysis

Preparatory testing for Total Microbial Plate Count, Total Yeast and Mold Plate Count;

Preparatory testing and Screening for E. coli, Salmonella species by Direct Inoculation and plate method;

Preparatory tests and Screening for Staphylococcus aureus, Pseudomonas aeruginosa by Direct Inoculation and Plate Method

Developed standard operating procedures (SOP) for analytical methods used in the laboratory

Practiced GMP and GLP

Perform Microbiological, chemical testing aseptically for ingredients, raw and processed products

University of Sheffield, UK Sep. 2009 – Dec. 2010

Graduate Student

Project: Studying overexpression of Adrenomedullin and its specific receptors on prostate cancer cell invasiveness

Performed bioassays (wound healing and MTT assays) to study the effect of overexpression of Adrenomedullin (ADM) and it specific receptors (ADM-2 and ADM-3) on migration and proliferation of PC-3 cells

Tested cell culture and sub culturing of PC-3 cell line, bacterial culture, plasmid isolation (midi-prep), DNA and RNA extraction/ quantification (nanodrop), bacterial transformation, RT-PCR (cDNA synthesis), gel electrophoresis, and cell electroporation

EDUCATION

Master of Science (MSc), Biopreservation 2015-2017

University of Alberta, AB, Canada (GPA: 3.8/4)

Master of Science (MSc), Molecular Medicine 2009-2010

University of Sheffield, UK, Merit

Bachelor of Science (BSc), Cellular and Molecular Biology 2001-2005

University of Shiraz, Iran, First class degree

References Are Available Upon Request

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