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Pavan Kumar Cherukuri, Ph.D.

Phone: 516-***-****. Email:ac3jma@r.postjobfree.com

Summary

• An interdisciplinary research scientist with seven years of experience in Pharmacology, Formulations and Chemistry.

• An efficient scientist with a proven track record in synthesis and purification of nanoparticles for biological use in in-vivo and in-vitro models systems.

• Scientist adept at handling wide variety of formulation and molecular biology techniques.

• A published scientist with 11 publications and 11 conference presentations.

• Demonstrated ability to efficiently prioritize a broad range of responsibilities in order to achieve maximum level of operating effectiveness.

Education:

Ph.D. in Biomedical Sciences (2009-2016), Old Dominion University, Norfolk, VA. B.Pharmacy (2003-2007), Osmania University, India

Technical Skills:

Pharmacological: Eukaryotic cells cultures (ovarian cancer cells, brain tumor cells, pancreatic cancer cells, colon cancer cells, Liver cancer cells), Stem cell culture, Prokaryotic cells culture

(Gram-positive and gram-negative bacteria), Breeding zebrafish and maintenance, Efflux kinetics/mechanism of cells using fluorescent dyes, dialysis, fixing cells (prokaryotic and Eukaryotic cells), Sterile technique, Tissue processor microtome, Histology of samples and staining, Western blot, Inhibitory concentration determination. DNA recombinant technology, Analysis of ions, Binding affinity of receptor and substrate using monoclonal antibody and antigen or proteins, ELISA, Immunohistochemistry (IHC) techniques. Assay development such as cancer stem cell identification (Nanoassay for real-time study of single cancer stem cells), viability of cells (prokaryotes and eukaryotes).

Pharmaceutical: Preparation of cell culture medium and buffer solution. Pipetting the solution using micropipettes single and multichannel pipette). Nano particle synthesis (2-100 nm), wet chemical synthesis, chemical replacement reaction, co-precipitation method, Rotary evaporation, centrifugation. Preparation of sample for NMR analysis

Analytical: Dark Field Optical Spectroscopy, Fluorescence spectroscopy, Single nanoparticle spectroscopy, UV Spectroscopy, Dynamic Light Scattering, Atomic Absorption Spectroscopy, Zeta- potential, Conductivity of solution. Transmission electron microscopy of bacteria cells, NMR sample 2

analysis, Freeze drying, Sonication, Flow cytometry and chromatography & Calorimetric analysis. HPLC

Microscopy/Imaging: Dark field optical microscopy, Bright field optical microscopy, Fluorescence microscopy, Single nanoparticle imaging, Single live cell imaging, Single live embryo imaging. Professional Experience:

Research Assistant (Jan 2009-Present), Old Dominion University, Norfolk, VA

• Experience in cell based assay development and other molecular and cellular biology methods like identification of cancer stem cells and single live cells.

• Experience in identifying cancer stem cells and single live cells.

• High performance liquid chromatography for analysis of amino acids and proteins

• Excellent understanding and working experience in cancer therapeutics, chemical synthesis and formulations, protein formulation.

• Experience in synthesizing and characterizing nano particles and protein therapeutics.

• Thorough understanding of chemical degradation pathways involved with formulations.

• Experience in analyzing therapeutic efficacy of different formulations in vitro (cancer cells, Prokaryotes, neurons) and in vivo tumor models.

• Gained expertise in publication writing, grant writing, reviewing, book editing, clinical protocol and SOP writing. Maintaining lab inventory both chemical and equipment, MSD, reagents and LIMS. Excellent in Ms-office, power point, and excel sheet.

• Data analysis using SPSS, matlab, Anova, regression analysis. Protein structure analysis

• Supervised undergraduate, interns for 5 years.

• Gained excellent communication skills while interacting with collaboration teams.

• Published 4 papers in high impact journals and presented 16 presentations in various national and international conferences.

Research Assistant (Oct 2008-Dec 2008), Old Dominion University, Norfolk, VA

• Gained expertise in chemical synthesis and reaction process monitoring along with analytical method development. HPLC analysis of environment samples

• Method developed for extraction of environmental samples.

• Chemical analysis of environmental samples.

Teaching Assistant, Chemistry laboratory, Organic chemistry and Biochemistry, (2009, 2014- 2015 and 2016) Old Dominion University, Norfolk, VA 3

Experience in teaching: Fundamentals of chemistry, Chemical reactions, Chemical kinetics, chemical bonding, Stoichiometry, Organic Chemistry, Thermodynamics, Gas laws, Emission spectroscopy, Analytical techniques (TLC), Chemical compound and Biodiesel synthesis, and DNA extraction. Pharmacy Technician (2007-2008), Hyderabad, India

Experience in compounding pharmaceuticals.

Experience in communicating with patients about pharmaceuticals. Publications

1. Lee, K. J.; Browning, L. M.; Nallathamby, P. D.; Desai, T.; Cherukuri, P. K.; Xu, X. H., In vivo quantitative study of sized-dependent transport and toxicity of single silver nanoparticles using zebrafish embryos. Chemical research in toxicology 2012, 25 (5), 1029-46. 2. Lee, K. J.; Nallathamby, P. D.; Browning, L. M.; Desai, T.; Cherukuri, P. K.; Xu, X. H., Single nanoparticle spectroscopy for real-time in vivo quantitative analysis of transport and toxicity of single nanoparticles in single embryos. The Analyst 2012, 137 (13), 2973-2986. 3. Browning, L. M.; Lee, K. J.; Cherukuri, P. K.; Nallathamby, P. D.; Warren, S.; Jault, J.-M.; Xu, X.- H. N., Single nanoparticle plasmonic spectroscopy for study of the efflux function of multidrug ABC membrane transporters of single live cells. RSC Advances 2016, 6 (43), 367**-*****. 4. Browning, L. M.; Lee, K. J.; Nallathamby, P. D.; Cherukuri, P. K.; Huang, T.; Warren, S.; Xu, X.-H. N., Single Nanoparticle Plasmonic Spectroscopy for Study of Charge-Dependent Efflux Function of Multidrug ABC Transporters of Single Live Bacillus subtilis Cells. The Journal of Physical Chemistry C 2016 10.1021/acs.jpcc.6b03313.

5. Nallathamby, P.D*; Cherukuri, P. K*; Xu, X.-H. N., “Study of Cytotoxic and Therapeutic Effects of Silver Nanoparticles on Ovarian Cancer Cells equal co-authorship}(In preparation) 6. Cherukuri, P. K; Nallathamby P.D; Browning, L.M: Perez, E; Xu, X.-H. N., “Study of Cytotoxic and Therapeutic Effects of Silver Nanoparticles on Brain Tumor Cells”, (In preparation). 7. Cherukuri, P. K ; Ding, F; Songkiatisak, P ; T. Huang, and X. Xu*, “Design and Study of Size- Dependent Therapeutic Effects of Silver Nanoparticles toward B. subtilis”, (In preparation). 8. Ding, F ; Cherukuri, P. K ; Songkiatisak,P T. Huang, and X. Xu*, “Design and Study of Size- Dependent Therapeutic Effects of Silver Nanoparticles toward Pseudomonas aeruginosa”, (In preparation).

9. Songkiatisak, P; Cherukuri, P. K ; F. Ding, T. Huang and X. Xu*, “Sized dependent multivalency effects of silver nanoparticles conjugated with antibiotics on E. coli and its consequence on extensive filamentation”, (In preparation).

10. Cherukuri, P. K ; Songkiatisak, P ; T. Huang, and X. Xu*, “Design and Study of Size-Dependent Nano-drug Carriers Efflux function of ABC transporter of single live B.subtilis cells”, (In preparation). 11. Songkiatisak, P; Cherukuri, P. K ; T. Huang and X. Xu*, “Study of size dependent Nano-drug Carriers Efflux function of multidrug ABC membrane transporter of single live E.coli cells ”, (In preparation).

Poster Presentations

1. X. Xu*, K. Lee, L. Browning, P. Nallathamby, P. Cherukuri, “Single nanoparticle spectroscopy for quantitative analysis of nanotoxicity”, Proc. of Pittcon, Philadelphia, 2013. 2. L. Browning, K. Lee, P. Nallathamby, P. Cherukuri, E. Perez, X. Xu

*, “Study of charge-dependent

efflux function of multidrug membrane transporters in single live cells”, Pittcon’2014, Chicago. 3. P. Songkiatisak, M. Johnson, L. Browning, P. Cherukuri, and X. Xu*, “In Vivo Study of Toxic Effects of Silver Ions on Embryonic Development”, Pittcon, Chicago, 2014. 4

4. P. Songkiatisak, M. Johnson, L. Browning, P. Cherukuri, S. Warren, N. Gonda and X. Xu*, “Study of Acute Toxic Effects of Nanoparticles on Embryonic Development”, Pittcon, New Orleans, 2015. 5. M. Johnson, P. Songkiatisak, P. Cherukuri, L. Browning, N. Gonda, and X. Xu*, “In vivo Study of Nanoparticles on Embryonic Neurological Development”, Pittcon, New Orleans, 2015. And 11 more.

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