Junior Scientist

*(*)

A.M. Noah Curriculum Vitae

Curriculum vitae

Name: Alexandre NOAH MBOENE

Nationality: Cameroonian

Address: Department of Biochemistry,

Faculty of Sciences, PO Box 24157 Douala-Cameroon. Phone: +237-***-**-**-**

E-mail: ***********@*****.***

LinkedIn: https://www.linkedin.com/pub/alexandre-noah/63/147/bb3/en Google Scholar: http://scholar.google.ca/citations?user=MH5IaVkAAAAJ&hl=en Major research interest: Plant Biochemistry, Molecular Biology, Systems Biology. EDUCATION

01/2010–23/2016

PhD in Plant Biotechnology, University of Yaoundé I PROTEOMICS ANALYSES OF EMBRYOGENESIS IN Theobroma cacao (L.) Using proteomics as an analytical system, my PhD project aimed to uncover the intrinsic molecular mechanism that governs the transition from embryo to healthy seedling of Theobroma cacao (the chocolate tree). This project was primarily orientated toward the development of somatic embryogenesis as an alternative method for large scale propagation of cocoa elite genotypes.

Key publication: Noah MA, Niemenak N, Sunderhaus S, Haase C, Omokolo ND, Winkelmann T and Braun HP

(2013). Comparative proteomic analysis of early somatic and zygotic embryogenesis in Theobroma cacao L. Journal of Proteomics, 78: 123–133.

See the full list of publications and presentations on the related document. 09/2007–10/2009 Master in Plant Biotechnology

Main subjects studied:

- Molecular biology and biochemical techniques for assaying biological compounds such as sugars, amino acids, phenols, peroxidases and several other enzymes

- Research methodology and scientific drafting

- Final dissertation: Mass Propagation of cocoyam (Xanthosoma sagittifolium) in Temporary Immersion Bioreactor

09/2006–10/2008 Master in education

Main subjects studied:

- General knowledge on all the domain of biology and geology

- Conception of teaching content and management of a class room

- School administration

- Ethic and deontology of education

09/2003–06/2006 Bachelor in Plant Biology

Main subjects studied: Botany; Genetics; Plant Physiology and Experimental Biochemistry. RESEARCH SKILLS

During the course of my research I have built up a wealth of techniques including:

- Plant tissue culture: Somatic embryogenesis, micro-propagation, temporary immersion bioreactor

- Mammalian cell culture and transfection

- RNA extraction, qPCR and sample preparation for RNA sequencing

- Plant hormone metabolite extraction and purification (auxins and cytokinins)

- Biochemistry: Western blot, Protein chemistry, isolation and purification techniques, comparison of electrophoresis gel with DELTA 2D software, stage tip for peptide fractionation and hormonal metabolite purification, formulation of novel methodologies

- Proteomics: high-throughput protein identification on Orbitrap analyzer

- Bioinformatics: Experienced with Proteomic large dataset analysis with PERSEUS and CYTOSCAPE; Computational analysis of protein motifs and protein-protein interactions. I have successfully taken the online course Bioinformatics Methods II taught by Dr Nicholas J. Provart, Department of Cell & Systems Biology at the University of Toronto. In November, 2015, I participated in the training workshop organized by the European Institute of Bioinformatics (EBI) at Umea University, Sweden. 2(3)

A.M. Noah Curriculum Vitae

COMMUNICATION SKILLS

- Excellent written ability developed through writing my thesis and co-writing papers and posters for presentation.

- Good oral presentation skills: I have presented my work several times at scientific conferences including a Talk at the Annual meeting of the Canadian Society of Plant Biologists and a Poster at the Annual meeting of the American Society of Plant Biologists in July 2015.

- President, of the Environmental Club of the Higher Teachers’ Training College, University of Yaoundé I (2007–2008).

- Involved in National Science Week to promote science to the local public. CAREER SUMMARY

05/2009 till date High School Teacher of biology, employed by the Ministry of Secondary Education-Cameroon Teaching biology to high School students from Fom one to Upper six (from 12 to 19 years old) 07/2015–11/2015 Visiting Researcher at the Umeå Plant Science Centre (UPSC), Department of Forest Genetics and Plant Physiology, Sveriges lantbruksuniversitet, SE-901 83 Umeå, Sweden. Mentored by Prof. Karin Ljung, E-mail: *****.*****@***.**

09/2012–09/2013 Exchange PhD Student at the University of Ottawa, Faculty of Medicine, Department of Biochemistry, Microbiology and Immunology, 451 Smyth, Ottawa ON K1H8M5 Canada. Internship held on High-throughput Proteomics analysis of cacao seed germination.

09/2011–11/2011 Visiting Student at the Abteilung Pflanzenproteomik, Institut für Pflanzengenetik, Naturwissenschaftliche Fakultät, Leibniz Universität, Herrenhäuser Straße 2 D-30419 Hannover Germany. Internship held on Comparative Proteomics analysis of Somatic versus Zygotic embryos of Theobroma cacao. AWARDS AND FELLOWSHIPS

05/2014 Travel Award of the Canadian Society of Plant Biology. 01/2015 Travelling Fellowships Grant to visit UPSC Funded by The Company of Biologists Limited, Bidder Building, Station Road, Histon, Cambridge, CB24 9LF. 02/2015 Travel Award of the American Society of Plant Biology. 07/2012

Canadian Commonwealth Scholarship Program

Funded by the Canadian Bureau of International Education and the Department of Foreign Affairs and International Trade.

07/2011

Wood-Whelan Research Fellowship

Funded by the International Union of Biochemistry and Molecular Biology (IUBMB). SCIENTIFIC SOCIETIES AFFILIATION

1. Member of the American Society of Plant Biologists 2. Member of the Canadian Society of Plant Biologists 3. Member of the Cameroon Biosciences Society

PUBLICATIONS / CONFERENCES

1. Noah MA, Elisma F, Ning Z, Chatfield PS, Omokolo ND, Figeys D and Niemenak N (2016). Proteomic-based System approach to map regulatory networks of germination and seedling establishment in Theobroma cacao L. Manuscript submitted at Journal of Proteome Research.

2. Niemenak N and Noah MA (2016). Progress toward the cacao zygotic and somatic embryo proteome. In Book of Abstract, the Symposium "Frontiers of Cacao Science and Technology for Cacao Quality, Productivity and Sustainability" The PennState, Huck Institute of the Life Sciences May 31th-June 2, 2016. 3. Makondy ARE, Noah MA and Niemenak N (2016). Somatic embryo germination and root development in cacao

(Theobroma cacao) and evaluation of some markers associated with secondary rhizogenesis. Syllabus Review, Sci. Ser., 6: 58-68.

4. Noah MA, Elisma F, Ning Z, Chatfield PS, Omokolo ND, Figeys D and Niemenak N (2015). A Proteomic Approach to Map Regulatory Networks Controlling Germination and Seedling Establishment in Therobroma cacao L. In Book of Abstact, Annual meeting of the American Society of Plant Biologists. Minneapolis Minnesota, 26-30 July 2015. 5. Noah MA, Niemenak N, Sunderhaus S, Haase C, Omokolo ND, Winkelmann T and Braun HP (2013). Comparative proteomic analysis of early somatic and zygotic embryogenesis in Theobroma cacao L. Journal of Proteomics, 78: 123–133. 3(3)

A.M. Noah Curriculum Vitae

6. Niemenak N, Noah MA and Omokolo ND (2013). Micropropagation of Xanthosoma sagittifolium in temporary imersion system. Plant Biotechnology Report, 7: 383-390. 7. Noah MA, Ning Z, Elisma F, Omokolo ND, Niemenak N and Figeys D (2013). A proteomics view of germination and seedling establishment in Theobroma cacao L. In Book of Abstact, Annual meeting of the Canadian Society of Plant Biologists. Québec City, 25-28 June 2013.

8. Fotso B, Noah MA, Minyaka E, Effa OP, Niemenak N. et Omokolo ND (2010). Physiologie de la germination des embryons somatiques de deux clones récalcitrants de cacaoyer (Theobroma cacao L.) en système d’immersion temporaire. In Book of Abstact, 17th annual Conference of the Cameroon Biosciences Society. Banganté, 2-4 December 2010. 9. Noah MA, Minyaka E, Effa OP and Niemenak (2009). Somatic embryogenesis of six recalcitrant genotypes of Theobroma cacao L. In Book of Abstact, 16thAnnual Conference of the Cameroon Biosciences Society. Yaoundé, 3-5 December 2009.

10. Noah MA, Effa OP, Minyaka E et Niemenak N (2009). Micropropagation du macabo dans le système d’immersion temporaire. In Cameroon Biosciences Society Proceedings: 15: 126-136. REFEREES

Prof. Niemenak Nicolas, University of Yaoundé I, Higher Teachers’ Training College, Department of Biological Science, PO Box 47 Yaoundé-Cameroon, Phone +237-***-***-***, E-mail: ********@*****.***

Prof. Figeys Daniel, University of Ottawa, Canada Research Chair in Proteomics and Systems Biology, Phone +1-613-***-****, ext. 8674, E-mail: *******@*******.**

Prof. Dr. Braun Hans-Peter, Leibniz Universität Hannover,Abteilung Pflanzenproteomik, Institut für Pflanzengenetik, Naturwissenschaftliche Fakultät, Phone +49-051*-***-****, E-mail: *****@*******.***-********.** Alexandre Noah Mboene, Ph.D.

University of Douala, Faculty of Sciences

Department of Biochemistry, PO Box 24157 Douala, Cameroon Tel: +237-***-**-**-**

E-mail: ***********@*****.***

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