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Engineering Professional Experience

Pittsburgh, Pennsylvania, United States
January 16, 2018

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Xujie Zhang

Career Object: A graduate Student of Biomedical Engineering, focusing on biomaterials. Possessing background knowledge on biochemistry and 3+ years of correlated lab experience. Seeking to leverage my expertise in the role of research and development.

5870 Bartlett Street, Pittsburgh, PA +1-323-***-**** EDUCATION

Carnegie Mellon University Pittsburgh, Pennsylvania Master of Biomedical Engineering (GPA: 3.6/4.0) Dec. 2017 Selected Coursework: Engineering Biomaterial, Biological Transport and Drug Delivery Molecular Biomechanics, Biomaterial Host Interactions in Regenerative Medicine Sun Yat-sen University Guangzhou, Guangdong

Bachelor of Engineering – Biomedical Engineering (GPA: 3.5/4.0) Jul. 2015 Selected Coursework: Biochemistry, Organic Chemistry, Polymer Chemistry and Physics Medical Physiology, Cell Biology, Instrument Analysis SKILLS

Computer Skill: Python, Matlab

Lab Skill: Cell culture and evaluation experiment (ELISA, CyQUANT assay, MTT assay, etc.) Organic Chemistry experiment (focus on organic molecule or material synthesis, and its characterization) Mechanical characterization experiment of biomaterials PROFESSIONAL EXPERIENCE

Allegheny General Hospital – Research assistant internship Summer 2017

• Isolated exosomes from hPEACs and 293T cell culture media using ultracentrifugation, and compared exosome quantities released per cell using Nanosight system

• Identified exosomes and detected protein level in exosome particles using western blot ACADEMIC PROJECTS

Term project: Nanoparticles for siRNA delivery to treat Graves’ Disease (proposal) Pittsburgh, Pennsylvania Carnegie Mellon University Fall 2016

• Designed a siRNA delivery system that will target plasma cells and reduce TSI level, which triggers Graves’ disease Term project: Protein-resistant miniature neural probe (proposal) Pittsburgh, Pennsylvania Carnegie Mellon University Fall 2016

• Cooperated with a team of four people to contrive a surface modified neural probe to reduce protein adsorption and inflammation in brain tissue, mainly took charge of in vitro and in vivo experiment design Graduation Design: Preparation Method of Compound Bioreactor Plate Guangzhou, Guangdong Sun Yat-sen University Spring 2015

• Designed model using SolidWorks and prepared polyvinyl chloride bioreactor plates with 3D printing technology, then coated the plates with PLGA membrane

• Conducted plasma modification experiment to increase hydroxyl density on the surface, then led gelatin grafting to the surface of plate using EDC/NHS cross-linking agent

• Evaluated stem cell viability on the modified plate using MTT assay and FDA staining, to test the practicability of its usage in towel bioreactor

Composite Material of Extracellular Matrix- Electrostatic Spinning Nanofiber Guangzhou, Guangdong Sun Yat-sen University Fall 2013

• Prepared polylactic acid fiber membrane using electrospinning, and cultured fibroblasts, which will secrete extracellular matrix in the nanofiber structure

• Removed fibroblasts using repetitive frozen method, enabled qualitative analysis of ECM growth on PLLA membrane

• Cultured stem cell on the ECM-PLLA composite material and observed cell proliferation and differentiation

• Applied and received patent for cell-culture application (No. CN201410116021.X) EXTRACURRICULAR ACTIVITIES

Member of Student Life Department, Student Union Nov. 2011 - Jun. 2012

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