Project System
Resume:
Personal Information:
Name: Wei Lin
Address: **** ******* ****** #***, *******, TX 77027
Email: **********@*****.***
Telephone No: 832-***-**** (cell)
Status: American Permanent Resident (Green card holder)
Education:
9/1998-12/2001 Ph.D., Molecular Virology, SunYat-sen University, China
9/1990-7/1993 M.S., Microbiology, Guangzhou Institute of Energy of Academia Sinica, Chinese Academy of Sciences, China
9/1984-6/1988 B.S., Biology, Huazhong Normal University, China
Research and Work Experiences:
Shenzhen FUNDOLAND Company, Shenzhen, China 2014-2017
Position: Researcher of biomedical project development
Department of Surgery, University of Texas-Health Science Center at Houston 2011-2013
Position Research Associate
Focused on mechanism study of intestinal hemorrhage shock
Project 1 To determine and compare in vivo the resuscitative capacity and biological effects of LP and FFP in a mouse model of controlled hemorrhage shock.
Project 2 The functional significance of PPAR-gamma to the protective effects of glutamine in the post ischemic gut.
Accountabilities Manage, conduct projects, design experiments and develop new protocols and training/mentoring of lab members & Medical School summer students
Achievements: Established lung tissue MPO assay, in vitro permeability method and accomplished the first projects. Resulted in one publication.
Related skills Knockout mouse models: breeding, maintenance and genotyping; lung and gut tissue processing including embedding, sectioning and HE & Immunostaining; Western blotting; RNA interference, etc.
Department of Internal Medicine, University of Texas-Health Science Center at Houston 2007-2010
Position Postdoctoral fellow
Focused on mechanism of signaling pathway of autoimmune disease
Project 1 Altered localization and function of DNA topoisomerase I in scleroderma fibroblasts and association of single nucleotide polymorphisms with fibronectin III in scleroderma patients.
Project 2 Analyze associations of single-nucleotide polymorphisms (SNPs) in the IL-1A, ARTS1 and IL23R genes with human ankylosing spondylitis.
Accountabilities Design and conduct gene function study in human autoimmunity diseases with in vitro cell culture model
Achievements: Established an adenovirus expression system for genes delivery and accomplished DNA topoisomerase I assay in scleroderma patients samples. Resulted in one publication.
Related skills qRT-PCR, molecular cloning, adenovirus expression system, immunoprecipitation, western blotting, mouse skin fibroblast primary culture and joint synoviocyte primary culture and etc.
Department of Molecular Virology and Microbiology, Baylor College of Medicine (2004-2007)
Position Postdoctoral Fellow
Focused on mechanism study of norovirus particles packaging
Project Norwalk virus-like particles (VLP) encapsulated nonstructural protein VPg
Accountabilities Manage and maintenance bio-reactor for VLP production, design and conduct research on VPg function of norovirus
Achievements: Testified that VPg could be encapsidated in Norwalk VLPs, found calcium was a key factor for VLP stability and it could result in a new therapeutics for norovirus infection (Summarized)
Related skills RNA isolation, molecular cloning, site-directed mutagenesis, recombinant baculovirus expression system, virions purification, production of Virus-like particles (VLP), calculation of virus titer using plaque assay, plaque purification, electron micrography, ELISA and etc.
Dept. of Biochemistry, Hong Kong University of Science and Technology, Hong Kong (2004-2004)
Position Research Associate
Project Expression of MCM (minichromosome maintenance) proteins in baculovirus system
Accountabilities Expression of recombinant MCM protein in baculovirus system
Achievements: Design and setting up baculovirus expression system in insect cells for the lab and finish the expression of recombinant MCM protein
Related skills Molecular cloning, Recombinant baculovirus expression system and etc.
State Key Laboratory for Biocontrol, School of Life Science, SunYat-sen University, China (1998-2001)
Position PhD student & Research Assistant
Project Characterization and localization of main capsid protein of Bombyx mori cytoplasmic polyhedrosis virus and sequencing and functional analysis of the single strand RNA virus- dengue virus.
Accountabilities Manage, design and conduct scientific research in biology and molecular biology of single strand RNA virus, supervision lab daily research activity and the training of lab members.
Achievements: Accomplished sequencing and function study of Bombyx mori cytoplasmic polyhedrosis virus and dengue virus. Submitted one virus gene sequence to GeneBank and resulted in three publications.
Related skills Molecular cloning, DNA sequence, site-directed mutagenesis, Protein expression in E.coli, Virions purification, Electron micrography
Expertise and experimental skills:
Molecular biology
RNA manipulation
PCR and RT-PCR
Molecular cloning
Site-directed mutagenesis
DNA sequence
Dot Blotting, Southern blotting, Northern blotting
In vitro transcription
Genotyping
Cell Biology and Immunology
Cell or tissue culture
Insect cell culture using bioreactor system
Gene and siRNA transfection
Immunosorbent electron microscopy
ELISA
Flow cytometry
Biochemistry
Protein expression in E.coli and recombinant baculovirus expression system
Adenovirus expression system
Protein separation and purification
Western Blotting
Immunoprecipitation
Virology
Virions purification
Production of Virus-like particles (VLP)
Measurement of virus titer using plaque assay
Plaque purification
Electron micrography
Animal experimental technology
Intraperitoneal (IP) injection
Mouse skin fibroblast primary culture and joint synoviocyte primary culture
Knock- out mice
Mouse genotyping
Mouse breeding and maintenance
Histology
Tissue embedding
Tissue section
HE & Immunostaining
Major publications:
1.Ban K, Peng Z, Lin W, Kozar RA. Arginine decreases peroxisome proliferator-activated receptor-γ activity via c-Jun. Mol Cell Biochem. 2012 Mar; 362(1-2):7-13.
2.Xiaodong Zhou (correspondence author), Lin W, Filemon K Tan, Shervin Assassi, Mavin J Fritzler, Xinjian Guo, Roozbeh Sharif, Tom Xia, Syeling Lai and Frank C Arnett. Decreased Catalytic Function with Altered Sumoylation of DNA Topoisomerase I in Scleroderma Fibroblasts. Arthritis Research & Therapy. 2011, 13:R128.
3.Chen S, Cheng L, Zhang Q, Lin W, Lu X, Brannan J, Zhou ZH, Zhang J. Genetic, biochemical, and structural characterization of a new densovirus isolated from a chronically infected Aedes albopictus C6/36 cell line. Virology, 2004, 318(1):123-133.
4.Zhang QF, Cui JM, Huang XJ, Lin W, Tan DY, Xu JW, Yang YF, Zhang JQ, Zhang X, Li H, Zheng HY, Chen QX, Yan XG, Zheng K, Wan ZY, Huang JC. Morphology and morphogenesis of severe acute respiratory syndrome (SARS) -associated virus. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao, 2003, 35(6):587-591.
5.Lin W, Yang W, Xu A, Liang Y, Chen S, Dai W and Zhang J. Sequencing of whole segment IV of Bombyx mori Cytoplasmic Polyhedrosis virus. Microbiology, 2003, 30(1): 41-44.(微生物学通报)
6.Lin W, Xu A, Yang W, Sun J, Lu X and Zhang J. Sequencing of genomic dsRNA segment V of Bombyx mori Cyto-plasmic Polyhedrosis virus. Chinese journal of virology, 2002, 18(4): 375-377.(中国病毒学学报)
7.Lin W, Wei J, Yang W and Zhang J. Cloning and sequencing of partial structure protein gene of Bombyx mori Cytoplasmic Polyhedrosis virus. Journal of Sun Yat-sen University (Natural Science Edition), 2001, 40(2): 75-77. (中山大学学报-自然科学版)
Personality:
I consider myself is diligent, earnest, perseverant, detail oriented and friendly easy going. I am very interested in biomedical and clinic translation research, especially about key human diseases. I have solid experience in molecular and cell biology, biochemistry, virology and relevant fields. I hope to do clinic relevant research and explore new approaches for human disease treatment with my knowledge and technology. I can work independently or play a role as a team member. I do believe that it is very important for big goal to have good relationship in a big team.
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