TABASSUM M. RAHMAN

* ***** **, ****** ** ***** M: 518-***-**** Email: ac2qtb@r.postjobfree.com

EDUCATION:

New York University Master of Science June 2006 in Recombinant DNA technology

Pace University Bachelor of Science June 2003 in Biology

TECHNICAL EXPERTISE:

●Immunoassays: ELISAs, CBA, Luminex assays, dendritic cell biology, primary cell isolation

●Imagestream and Flow Cytometry: Acquisition, sorting and analysis

●Molecular Biology: Gel electrophoresis, Easymag extraction, PCR, qPCR, cloning, sequence analysis, GenMark Assay, 16S Assay, Cepheid, DNA preparation for whole genome sequencing

●Microbiology: Bacterial transformation, selection, Isolation and Identification by MALDI-TOF

●Cell Biology: Recombinant protein expression, purification, SDS-PAGE, Western blot

●Cell Culture: tissue culture manipulation, primary cell differentiation, in vitro cell transfections

●Biosafety experience: Cell culture and in vitro-in vivo infection in BSL-2 and BSL-3 facilities.

●Others: microscopy, immunohistology, staining techniques

●Software applications: MS Office, GraphPad Instat, Prism, FlowJo, EndNote, LIMS

EXPERIENCES:

Health Research Inc. (NYSDOH Bacteriology lab at Wadsworth center) Albany, NY

Research Scientist 01/17-10/17

●Identify, Respond to and Prevent resistant pathogens for CDC’s Antibiotic Resistance Lab Network.

●Perform, develop, test Quality Assurance and validate molecular assays by TaqMan Real-time PCR.

●Detect bacteria by MALDI-TOF, extract DNA for 16s and whole genome sequencing

●Prepare bacterial cell lysates, assay controls and bulk mixes.

●Analyze, interpret and report test results promptly as per Laboratory protocol.

Health Research Inc. (NYSDOH Virology lab at Wadsworth center) Albany, NY

Assistant Research Scientist 01/16-12/16

●Process isolates received for various virus surveillance testing including accessioning samples, scheduling tests and banking for inventory.

●Perform molecular diagnostic tests by single or multiplex PCR assays and GenMark Assay.

●Conduct complete procedure of New York State Zika Virus surveillance testing by PCR.

●Analyze test result, schedule for retests and maintain test results in laboratory information system.

Albany Medical College Albany, NY

Senior Research Technician 09/09-01/16

●Conducted independent research on inflammasomes to identify the mechanisms of immune response against bacterial infection using Francisella and Listeria model systems.

●Used site directed mutagenesis and cloning method to create chimeric constructs and mutants evaluating the immunogenicity of different NLRs.

●Cell transfection followed by infection to reconstitute inflammasomes in HEK293T cells.

●Implemented immunoassays (cell proliferations, maturation, activation, antigen binding, ELISAs, CBA, Luminex assay, Flow cytometry) in support of preclinical programs.

●Coordinated in vivo studies and participated in the performance of immunizations, sampling and sample processing from small laboratory animals.

●Collected data through complex laboratory/scientific experiments, techniques, and procedures. Record, interpret and analyze data using scientific or statistical techniques.

●Performed routine lab and equipment maintenance, prepared and maintained culture media, reagents, cells; grow cultures. Set-up and operated various scientific apparatus; troubleshoot and calibrated instruments, maintained inventory levels for supplies.

●Performed supervisory and training duties involving students and new employees.

●Prepared technical reports, summaries, protocols, quantitative analyses and presentations on the project progress. Conducted literature reviews, Prepared manuscript materials for submission to granting agencies and for publication.

NYU Medical Center New York, NY

Graduate Research Assistant 09/05-06/06

●Worked on a project with rat cochlea to quantify expression of ASIC/Degenerin genes.

●Optimized the assays by analyzing relative primer binding efficiency, defining the correct primer and probe concentration and achieving optimal salt concentration.

●Analyzed data obtained from 7 different genes in the ASIC family for expression analysis.

New York City Department of Health New York, NY

Public Health advisor 02/04-06/05

●Reviewed patient logs in microbiology, pathology, serology and parasitology laboratories.

●Collected data for communicable disease surveillance activities at hospitals and clinics and reported findings to appropriate bureaus according to detailed protocols and epidemiology.

●Developed and monitored the quality control of laboratories in NYC by ensuring that SOP Manual is complete, up-to-date and in compliance with city, state and federal regulations.

Haskins Laboratory New York, NY

Research Assistant 06/02-06/03

●Performed research on Microsporidia by testing infected Rabbit Kidney cells with Polyamine drugs.

●Performed ELISA for drug screening.

●Performed associated procedures like spore count, inoculation, preparation of media and drugs.

PUBLICATIONS:

●Singh A, Rahman T, Bartiss R, Arabshahi A, Prasain J, Barnes S, Musteata FM, and Sellati TJ. Lipoxin A4, a 5-lipoxygenase pathway metabolite, modulates immune response during acute respiratory tularemia. J Leukoc Biol jlb.4A0815-365RR; doi:10.1189/jlb.4A0815-365RR

●Singh A, Rahman T, Malik M, Hickey AJ, Leifer CA, et al. (2013) Discordant Results Obtained with Francisella tularensis during In Vitro and In Vivo Immunological Studies Are Attributable to Compromised Bacterial Structural Integrity. PLoS ONE 8(3): e58513.

●Periasamy S, Singh A, Sahay B, Rahman T, Feustel PJ, Pham GH, Gosselin EJ, Sellati TJ. Development of tolerogenic dendritic cells and regulatory T Cells favors exponential bacterial growth and survival during early respiratory tularemia. J Leukoc Biol. 2011 Sep;90(3):493-507.

●Zarrella TM, Singh A, Bitsaktsis C, Rahman T, Sahay B, et al. 2011 Host-Adaptation of Francisella tularensis Alters the Bacterium's Surface-Carbohydrates to Hinder Effectors of Innate and Adaptive Immunity. PLoS ONE 6(7): e22335.

ACHIEVEMENTS: * The National Dean’s List Award

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