State University Quality Control
Resume:
Carmina C. Blanco
*** ******** *****, ********, ** 94044
Home Phone: 650-***-**** and Cell Phone: 650-***-****
e-mail: ***********@*****.***
PROFESSIONAL GOAL:
Desire to secure a research associate/senior research associate position in biotechnology/pharmaceutical company that encourages creativity and innovation in the field of Stem Cell Biology, Cell and Molecular Biology.
SUMMARY OF QUALIFICATIONS
Over twenty years research experience in the field of Stem Cell Biology, Cell and Molecular Biology
Ability to collaborate and work effectively in a team setting
Excellent organization and written communication skills
Proficient with the following computer programs: Microsoft Word, Excel and PowerPoint
EDUCATION
MBA – Marketing Management, graduated August 2017
Notre Dame de Namur University, Belmont, California
B. S. Biology - Cell and Molecular Biology, 1998
San Francisco State University, San Francisco, California
A.S. in Biotechnology, 1995
City College of San Francisco, San Francisco, California
WORK EXPERIENCE
Research Scientist I
Allevity/VistaGen Therapeutics, Inc. – South San Francisco, CA July 2005-April 2017
Maintained and cultured undifferentiated human Embryonic Stem cells (hES-2)
Differentiated hES-2 cells into cardiomyocytes using specific growth factors
Performed immunofluorescence (IF) via flow cytometry to characterize the differentiated cardiomyocytes using specific gene expression markers such as Cardiac Troponin T, MLC-2V, MCL-2A, and HCN4
Performed immunohistochemistry (IHC) using different antibodies to characterize different subtypes of cardiomyocytes.
Performed biological cell-based assays to measure toxicity level from small molecules
Performed basic molecular biology technique such as DNA isolation, RNA isolation, cloning and PCR
Research Associate II
Life Technologies Corporation, Carlsbad, CA September 2001 – July 2005
Maintained and cultured different mammalian cell lines
Performed basic molecular biology technique such as DNA isolation, RNA isolation, semi-quantitative PCR and cloning specifically Invitrogen’s Gateway recombination cloning technology
Performed transfections of generated recombinant adeno-associated viral vectors (rAAVs) carrying specific gene of interest in different fibroblast cell lines for virus production.
Performed purification of adeno-associated viral vectors using Cesium Chloride technique.
Performed transductions using purified adenovirus expressing a gene of interest in different cell lines.
Performed high-throughput cell based screening for validation of target sequence specific RNA stealthTM molecules and siRNAs
Research Associate I
UCSF, San Francisco, CA November 1999 – August 2001
Maintained fibroblast cell lines and undifferentiated human embryonic stem cells.
Performed basic molecular biology technique such as DNA isolation, RNA isolation, cloning and PCR
Performed in vivo studies by injecting undifferentiated hES-cells in mice.
Performed co-culturing of hearts from 9-day old mice with undifferentiated hES-cells
Performed phenotypic PCR amplifications of genomic DNA from clippings of mouse tails.
Quality Control/Microbiologist
Beckman Coulter, Palo Alto, CA June 1999 – November 1999
Maintained STO and CHO cell lines in culture.
Performed basic molecular biology technique such as DNA isolation, cloning and differential PCR to perform quality control of PCR reagents
Performed instrument testing such as scanners and sequencers
Research Assistant
San Francisco State University, San Francisco, CA June 1998 – June 1999
Performed transfection of plasmid DNA in mammalian cells
Maintained mammalian and insect cell lines
Performed general molecular biology techniques: isolated and purified plasmid DNA, SDS-PAGE, PCR, UV/Vis spectrophotometry, Southern and Western hybridizations
Performed enzymatic assays using scintillation counter
Performed general microbiological techniques: practicing good aseptic technique, pouring LB agar plates, streaking bacterial colonies, inoculating bacterial cultures, preparing laboratory standard solutions, adjusting pH of buffer solutions, performing transformation.
Summer Intern student
Lawrence Berkeley National Laboratory, Berkeley, CA June 1995 - August 1995
June 1994 - August 1994
Maintained mammalian cell culture.
Analyzed data from a non-radioactive DNA hybridization of spontaneous and irradiated mutants.
Performed basic molecular biological techniques such as agarose gel electrophoresis, DNA isolation and purification, and Southern hybridization.
PEER-REVIEWED PUBLICATION
Note: authorship listed as Carmina S. Catuar
Kristina C. Pfendler, Carmina S. Catuar, Juanito M. Meneses, and Roger A. Pedersen.
Overexpression of Nodal promotes differentiation of mouse embryonic stem cells into mesoderm and endoderm at the expense of neuroectoderm formation. Stem Cells and Development 14:162-172, 2005.
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