Carmina C. Blanco

*** ******** *****, ********, ** 94044

Home Phone: 650-***-**** and Cell Phone: 650-***-****

e-mail: ac2jnb@r.postjobfree.com

PROFESSIONAL GOAL:

Desire to secure a research associate/senior research associate position in biotechnology/pharmaceutical company that encourages creativity and innovation in the field of Stem Cell Biology, Cell and Molecular Biology.

SUMMARY OF QUALIFICATIONS

Over twenty years research experience in the field of Stem Cell Biology, Cell and Molecular Biology

Ability to collaborate and work effectively in a team setting

Excellent organization and written communication skills

Proficient with the following computer programs: Microsoft Word, Excel and PowerPoint

EDUCATION

MBA – Marketing Management, graduated August 2017

Notre Dame de Namur University, Belmont, California

B. S. Biology - Cell and Molecular Biology, 1998

San Francisco State University, San Francisco, California

A.S. in Biotechnology, 1995

City College of San Francisco, San Francisco, California

WORK EXPERIENCE

Research Scientist I

Allevity/VistaGen Therapeutics, Inc. – South San Francisco, CA July 2005-April 2017

Maintained and cultured undifferentiated human Embryonic Stem cells (hES-2)

Differentiated hES-2 cells into cardiomyocytes using specific growth factors

Performed immunofluorescence (IF) via flow cytometry to characterize the differentiated cardiomyocytes using specific gene expression markers such as Cardiac Troponin T, MLC-2V, MCL-2A, and HCN4

Performed immunohistochemistry (IHC) using different antibodies to characterize different subtypes of cardiomyocytes.

Performed biological cell-based assays to measure toxicity level from small molecules

Performed basic molecular biology technique such as DNA isolation, RNA isolation, cloning and PCR

Research Associate II

Life Technologies Corporation, Carlsbad, CA September 2001 – July 2005

Maintained and cultured different mammalian cell lines

Performed basic molecular biology technique such as DNA isolation, RNA isolation, semi-quantitative PCR and cloning specifically Invitrogen’s Gateway recombination cloning technology

Performed transfections of generated recombinant adeno-associated viral vectors (rAAVs) carrying specific gene of interest in different fibroblast cell lines for virus production.

Performed purification of adeno-associated viral vectors using Cesium Chloride technique.

Performed transductions using purified adenovirus expressing a gene of interest in different cell lines.

Performed high-throughput cell based screening for validation of target sequence specific RNA stealthTM molecules and siRNAs

Research Associate I

UCSF, San Francisco, CA November 1999 – August 2001

Maintained fibroblast cell lines and undifferentiated human embryonic stem cells.

Performed basic molecular biology technique such as DNA isolation, RNA isolation, cloning and PCR

Performed in vivo studies by injecting undifferentiated hES-cells in mice.

Performed co-culturing of hearts from 9-day old mice with undifferentiated hES-cells

Performed phenotypic PCR amplifications of genomic DNA from clippings of mouse tails.

Quality Control/Microbiologist

Beckman Coulter, Palo Alto, CA June 1999 – November 1999

Maintained STO and CHO cell lines in culture.

Performed basic molecular biology technique such as DNA isolation, cloning and differential PCR to perform quality control of PCR reagents

Performed instrument testing such as scanners and sequencers

Research Assistant

San Francisco State University, San Francisco, CA June 1998 – June 1999

Performed transfection of plasmid DNA in mammalian cells

Maintained mammalian and insect cell lines

Performed general molecular biology techniques: isolated and purified plasmid DNA, SDS-PAGE, PCR, UV/Vis spectrophotometry, Southern and Western hybridizations

Performed enzymatic assays using scintillation counter

Performed general microbiological techniques: practicing good aseptic technique, pouring LB agar plates, streaking bacterial colonies, inoculating bacterial cultures, preparing laboratory standard solutions, adjusting pH of buffer solutions, performing transformation.

Summer Intern student

Lawrence Berkeley National Laboratory, Berkeley, CA June 1995 - August 1995

June 1994 - August 1994

Maintained mammalian cell culture.

Analyzed data from a non-radioactive DNA hybridization of spontaneous and irradiated mutants.

Performed basic molecular biological techniques such as agarose gel electrophoresis, DNA isolation and purification, and Southern hybridization.

PEER-REVIEWED PUBLICATION

Note: authorship listed as Carmina S. Catuar

Kristina C. Pfendler, Carmina S. Catuar, Juanito M. Meneses, and Roger A. Pedersen.

Overexpression of Nodal promotes differentiation of mouse embryonic stem cells into mesoderm and endoderm at the expense of neuroectoderm formation. Stem Cells and Development 14:162-172, 2005.

Contact this candidate