Phone: 630-***-****

Email: ac2izv@r.postjobfree.com

www.linkedin.com/in/GrantGucinski

SUMMARY Ph.D. level Biochemist pursuing a Research & Development or Process Development position utilizing analytical, technical, management, and communication skills

• 10 years of research experience in the field of biochemistry with an emphasis on microbiology

• 9 years of experience with purification and characterization of DNA, RNA, and recombinant proteins

• Extensive management skills demonstrated by holding leadership and supervisory positions

• Excellent technical writing and communication skills proven by 4 publications and 5 conference presentations EDUCATION Ph.D. Biochemistry and Molecular Biology, University of California, Santa Barbara, CA 10/2017 (Expected) GPA: 3.96

Dissertation: “Delivery and Activity of Contact-Dependent Growth Inhibition Toxins” Advisor: Christopher Hayes, Ph.D.

B.A. Chemistry, Pomona College, Claremont, CA 05/2010 TECHNICAL SKILLS Experimental: FPLC (IMAC, SEC, IEC), HPLC, LC-MS, Mass Spectrometry, Enzyme Kinetics, SDS-PAGE, FACS, Molecular Cloning, DNA/RNA Extraction, Western blotting, Northern blotting, Fluorescence Microscopy, Circular Dichroism, NMR Spectroscopy, Cell-Based Assays Certifications: Biosafety Level 2 (BSL-2), Bloodborne Pathogens, Radiation Safety Computer: PyMol, ApE, ExPASy, BLAST, Microsoft Office Suite Lab Management: Standard Operating Procedure (SOP) writing, instrument training and maintenance Language: Bilingual in English and Spanish

PROFESSIONAL EXPERIENCE University of California, Santa Barbara, CA 09/2011 – Current Graduate Research Assistant, Biomolecular Science and Engineering

• Identified membrane translocation pathways utilized by contact-dependent growth inhibition systems

• Discovered novel activities of uncharacterized contact-dependent growth inhibition toxins from Gram- negative bacteria

• Managed lab operation by developing SOP’s and providing instrument training and maintenance

• Mentored 3 undergraduate researchers through instrument training, lectures, and discussions

• Presented research at 5 scientific conferences and published 3 articles in high-impact journals Graduate Teaching Assistant, Department of Molecular, Cellular, and Developmental Biology

• Developed curriculum for the senior-level microbiology lab class and trained ~90 students in modern microbiological techniques

• Led review sessions and elaborated difficult concepts to students of various knowledge levels

• Coordinated a graduate student seminar class for the development of presentation skills Pomona College, Claremont, CA 10/2007 – 08/2011

Research Assistant

• Developed FPLC purification method, optimized crystallization conditions, and determined crystal structure of toluene monooxygenase

• Studied the Escherichia coli ferrous iron transport system

• Supervised biochemistry and organic chemistry laboratory classes and ensured safety Grant Gucinski

LEADERSHIP EXPERIENCE Officer, Graduate Union of Molecular Biology Investigators 08/2012 – 06/2017 BMSE & MCDB, UC Santa Barbara

• Coordinated outreach events to foster collaboration amongst departments

• Led seminars to prepare students for qualifying exams and thesis defenses

• Represented graduate students in discussions with faculty and administration pertaining to stipend and other graduate student concerns

Student Liason 08/2008 – 05/2010

Department of Chemistry, Pomona College, CA

• Coordinated outreach events to engage prospective majors

• Interacted with faculty in the development of core curriculum PUBLICATIONS 1. Michalska, K,* Gucinski, GC,* Garza-Sánchez, F, Johnson, PM, Stols, L, Eschenfeldt, WH, Babnigg, G, Low, DA, Goulding, CW, Joachimiak, A, Hayes, CS. (2017) A novel antibacterial toxin exploits elongation factor Tu (EF- Tu) to cleave tRNA containing guanine discriminator nucleotides. Nucleic Acids Research, gkx700, https://doi.org/10.1093/nar/gkx700 *equal contribution 2. Johnson, PM, Gucinski, GC, Garza-Sánchez, F, Wong, T, Hung, L, Hayes, CS, Goulding, CW. (2016) Functional diversity of cytotoxic tRNase/immunity protein complexes from Burkholderia pseudomallei. J. Biol. Chem. 291:193**-*****. https://doi.org/10.1074/jbc.M116.736074 3. Willett, JLE,* Gucinski, GC,* Fatherree, JP, Low, DA, Hayes, CS. (2015) Contact-dependent growth inhibition toxins exploit multiple independent cell-entry pathways. PNAS, 112(36), 113**-*****.*equal contribution http://www.pnas.org/content/112/36/11341.long

• Highlighted in Nature Reviews Microbiology: http://www.nature.com/nrmicro/journal/v13/n10/full/nrmicro3554.html 4. Song, WJ, Gucinski, GC, Sazinsky, MH, Lippard, SJ. (2011) Tracking a defined route for O2 migration in a dioxygen-activating diiron enzyme. PNAS, 108(36), 147**-*****. http://www.pnas.org/content/108/36/14795.full

PRESENTATIONS 1. “E. coli NC101 Contact-dependent growth inhibition toxin is a tRNase that requires EF-Tu and EF-Ts” (Poster) Molecular Genetics of Bacteria and Phages Meeting, Madison, WI, August 8-12, 2016.

• Poster contest finalist

2. “Structure and activity of an E. coli contact-dependent growth inhibition toxin that requires ribosomal elongation factors EF-Tu and EF-Ts” (Poster) Mechanisms of Bacterial Toxin Delivery Symposium, Santa Barbara, CA, June 24, 2016.

3. “Structure and activity of an E. coli contact-dependent growth inhibition toxin that requires ribosomal elongation factors EF-Tu and EF-Ts” (Poster) Molecular Genetics of Bacteria and Phages Meeting, Madison, WI, August 4-8, 2015.

4. “Identification of inner-membrane proteins required for contact-dependent growth inhibition (CDI)” (Talk) West Coast Bacterial Physiologists Annual Asilomar Conference, Pacific Grove, CA, December 12-14, 2014. 5. “Identification of inner-membrane proteins required for contact-dependent growth inhibition (CDI)” (Poster) Molecular Genetics of Bacteria and Phages Meeting, Madison, WI, August 5-9, 2014.

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