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Flow Cytometry, DNA/RNA isolation, Q-PCR, PCR, western blot, cell cult

Fair Lawn, New Jersey, 07410, United States
November 12, 2017

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Home address: **-*** ******* ****, **** Lawn, New Jersey 07410

Immigration status: US citizen

Contact information: 201-***-**** (cell)

212-***-**** (office)


Linkedin: Zinaida Dubeykovskaya


Master of Science in Biochemistry,

Moscow State University, Moscow, Russia

Ph.D. in Biochemistry,

Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia


2004-Current: Associate Research Scientist at Irvine Cancer Research Center of Columbia University, 1130 St. Nicholas Avenue, Room 901,New York, NY, 10032


Anti-inflammatory and anti-tumor function of spasmolytic polypeptide/trefoil factor 2 (SP/Tff2) in colon and gastric cancer.


1.Design, plan and execute experiments to examine the role of TFF2 in gastric and colon cancer by using following techniques:

Mouse models: DSS-induced colitis, gastric, colon and liver cancer, maintaining mice colonies, genotyping

FACS analysis of immune cells populations from mouse immune, gastric and colon tumor tissues

phenotypic and functional characterization of myeloid-derived suppressive cells (arginase-, ROS- activity, immunosuppressive activity)

isolation myeloid and T-cells from spleen and bone marrow for in vitro culture and adoptive cells transfer via tail vein injection

Purification and functional characterization of recombinant mouse TFF2 and its derivates in tests in vitro and in vivo in mouse cancer models

Adenovirus-mediated gene therapy: adenovirus production, purification and tail vein injection

Western blotting, PCR, RT-PCR

Immunochistochemical staining of frozen tissue sections

Mouse bone marrow transplantation and analysis of mouse chimeras

Computer skills: Microsoft Word, Adobe Photoshop, Power Point, Excel, GraphPadPrism

analyze, summarize and present data, writing manuscript for peer-reviewed journals

Supervise students


Mouse models of colon, gastric and liver cancer

Multi (six)-color flow cytometry analysis

ELISA, SDS-PAGE electorphoresis & Western blotting, immunohistochemistry

Purification and characterization recombinant proteins from eukaryotic, bacterial and insect hosts and proteins from natural sources like human blood, snake venom, honey bee venom

Cultivation of eukaryotic and primary mouse cells (T-cells, macrophages, myeloid cells)

Plasmid DNA and mRNA isolation, PCR amplification, RT-PCR analysis

Chromatography: ion exchange, hydrophobic interaction, size exclusion, hydroxylapatite, immunoaffinity and affinity (metallochelate, immobilized dyes)


29. Hayakawa Y, Sakitani K, Konishi M, Asfaha S, Niikura R, Tomita H, Renz B. W., Tailor Y, Macchini M, Middelhoff M, Jiang Z, Tanaka T, Dubeykovskaya Z. A, Kim W, Chen X, Urbanska A M, Nagar K,., Westphalen C B., Quante M, Lin C-S, Gershon M D, Hara A, Zhao C-M, Chen D,. Worthley D L, Koike K and Timothy C. Wang. Nerve growth factor promotes gastric tumorigenesis through aberrant cholinergic signaling. Cancer Cell, 2017, Jan 9;31(1):21-34.

28. Dubeykovskaya ZA, Si Y, Chen X, Worthley DL, Renz BW, Urbanska AM, Hayakawa Y, Xu T, Westphalen CB, Dubeykovskiy A, Chen D, Friedman RA, Asfaha S, Nagar K, Tailor Y, Muthupalani S, Fox JG, Kitajewski J, Wang TC. Neural innervation stimulates splenic TFF2 to arrest myeloid cell expansion and cancer. Nat. Commun. 2016 Feb 4; 7:10517

27.Hayakawa Y., Ariyama H, Stancikova J, Sakitani K, Asfaha S, Renz BW, Dubeykovskaya ZA, Shibata W, Wang H, Westphalen CB, Chen X, Takemoto Y, W, Khurana SS, Tailor Y, Nagar K, Tomita H, Hara A, Sepulveda AR, Setlik W, Gershon MD, Saha S, Ding L, Shen Z, Fox JG, Friedman RA, Konieczny SF, Worthley DL, Korinek V, Wang TC. Mist1 expressing gastric stem cells maintain the normal and neoplastic gastric epithelium and are supported by perivascular stem cell nich. Cancer Cell. 2015, Dec 14; 28(6):800-14.

26. Judd LM, Chalinor HV, Walduck A, Pavlic DI, Däbritz J,

Dubeykovskaya Z, Wang TC, Menheniott TR, Giraud AS. TFF2 deficiency

exacerbates weight loss and alters immune cell and cytokine profiles in DSS

colitis, and this cannot be rescued by wild-type bone marrow. Am J Physiol

Gastrointest Liver Physiol. 2015 Jan 1;308(1):G12-24.

25.Hayakawa Y, Jin G, Wang H, Chen X, Westphalen CB, Asfaha S, Renz BW, Ariyama H, Dubeykovskaya ZA, Takemoto Y, Lee Y, Muley A, Tailor Y, Chen D, Muthupalani S, Fox JG, Shulkes A, Worthley DL, Takaishi S, Wang TC. CCK2R identifies and regulates gastric antral stem cell states and carcinogenesis. Gut. 2015 Apr; 64: 544-653

24.Asfaha S., Dubeykovskiy A., Tomita H., Yang X., Stokes S., Shibata W., Friedman R, Ariyama H., Dubeykovskaya Z., Muthupalani S., Ericksen R., Frutcht H., Fox J.G., Wang T.C. Mice that express human interleukin- 8 have increased mobilization of immature myeloid cells, which exacerbates inflammation and accelerates colon carcinogenesis. Gastroenteroliogy, 2013, 144(1), 155-66.

23.Shibata W., Ariyama H., Westphalen C.B., Muthupalani S., Asfaha S., Dubeykovskaya Z., Quante M., Fox J.G., Wang T.C. Stromal cell-derived factor-1 overexpression induces gastric dysplasia through expansion of stromal myofibroblasts ans epithelial progenitors. Gut, 2013, 62(2), 192-200.

22.Quante M, Bhagat G, Abrams JA, Marache F, Good P, Lee MD, Lee Y, Friedman R, Asfaha S, Dubeykovskaya Z., Mahmood U, Figueiredo JL, Kitajewski J, Shawber C, Lightdale CJ, Rustgi AK, Wang TC. Bile Acid and inflammation activate gastric cardia stem cells in a mouse model of barrett-like metaplasia. Cancer Cell, 2012, Jan 17; 21(1):36-51.

21.Takaishi S., Shibata W., Tomita H., Jin G., Yang X., Ericksen R., Dubeykovskaya Z., Asfaha S., Quante M., Betz K.S., Shulkes A., Wang T.C. In vivo analysis of mouse gastrin gene regulation in enhanced GFP BAC transgenic mice. Am J Physiol Gastrointest Liver Physiol. 2011, Feb:300(2):G334-44.

20.Okomura T., Ericksen R.E., Takaishi S., Wang S.S.W., Dubeykovskiy Z., Shibata W., Betz K., Mathupalani S., Gogers A.B., Fox J.G., Rustgi A.K., Wang T.C. K-ras mutation targeted to gastric tissue progenitor cells results in chronic inflammation, an altered microinvaronment, and progression to intraepithelial neoplasia. Cancer Res., 2010, 70(21):8435-45.

19.Tu S.P., Chi A.L., Ai W., Takaishi S., Dubeykovskaya Z., Quante M., Fox J.G., Wang T. C. P53- inhibition of AP-1 dependent TFF2 expression induces apoptosis and inhibits cell migration in gastric cancer cells. Am J Physiol Gastrointest Liver Physiol. 2009 Aug; 297(2):G385-96.

18.Takaishi S., Tu S.P., Dubeykovskaya Z., Whary M.T., Muthupalani S., Rickman B.H., Rogers A. B., Lertkowit N., Varro A., Fox J.G., Wang T.C. Gastrin is an essential cofactor for Helicobacter-associated gastric corpus carcinogenesis in C57BL/6 mice. Am. J.Pathology, 2009 July; 175:365-375

17.Dubeykovskaya Z., Dubeykovskiy A., Solal-Cohen J., Wang T.C. Secreted trefoil factor 2 activates the CXCR4 receptor in epithelial and lymphocytic cancer cell lines. J Biol Chem, 2009, 286, 3650-62.

16.Dubeykovskiy A.N., Nguyen T., Dubeykovskaya Z., Lei S., Wang T.C. Flow cytometric detection of progastrin interaction with gastrointestinal cells. Regul. Peptide, 2008, 151, 106-14.

15.Tu S., Chi A.L., Lim S., Cui G., Dubeykovskaya Z., Ai W., Fleming J.V., Takaishi S., Wang T. C. Gastrin regulates the TFF2 promoter through gastrin-responsive cis-acting elements and multiple signaling pathways. Am J. Physiol. Gastrointest. Liver. Physiol. 2007, 292, G000-G000.

14.Ksenzenko V.N., Ivashina T.V., Dubeikovskaya Z.A., Ivanov S.G., Nanazashvili M.B, Druzhinina T.N., Kalinchuk N.A., Shibaev V.N. The pssA gene encodes UDP:glucose-poleprenilphosphate glucosyltransferase responsible for initiation exopolysaccharyde biosynthesis in Rhizobium leguminozarum. Russian Journal of Bioorganic Chemistry, 2007, 33, 150-155.

13.Stark J., Cohet N., Gonnet C., Sarrazin S, Doubeikovskaia Z., Doubeikovski A., Verger A., Duterque-Coquillaud M., Morle F. Functional cross-antagonism between transcription factors Fli-1 and EKLF. Mol. Cell Biol. 2003, 23:1390-1402.

12.Doubeikovskaia Z, Aries A, Jeannesson P, Morle F, Doubeikovski A. Purification of human recombinant GATA-1 from bacteria: implication for protein-protein interaction studies. Protein Expr. Purif. 2001, 23, 426-31.

11. Derbyshev V.V., Petrenik I.F., Doubeikovski Z.A., Aslanyan E.M., Kiselev Yu.B., Dobritsa A.P. The technological characteristics of a recombinant Pseudomonas aeurofaciens strain, a producer of Lepidoptera protein toxin from Bacillus thuringiensis. Biothechnology (Russ):1999, 5, 3-10.

10.Doubeikovski A., Uzan G., Doubeikovski Z., Prandini M.-H., Gisselbrecht S., Dusanter- Fourt I.. Trombopoietin-induced expression of the GPIIb gene involves the transcription factor PU.1/SPI-1 in UT7-MPL cells. J Biol Chem, 1997, 272: 243**-*****.

9.Morceau F., Aries A., Lahlil R., Chenais B., Devy L., Doubeikovskaya Z.A., Doubeikovski A., Jardillier J.-C., Trentsaux C., Jeannesson P. Molecular Mechanisms of antracyclines-mediated differentiation of human erythroleukemic cells. In: Differentiation Therapy, 1995, 10, 477-481, ed. Samuel Waxman, M.D

8.Kuzina L.V., Koretskaia N.G., Trofimenko A.F., Dubeikovskaya Z.A., Dobritsa A.P. Design of recombinant strains Bacillus thuringiensis efficient against insect of Lepidoptera and Coleoptera orders. Biotechnology (Russ), 1994, 7, 15-17.

7.Sever I.S., Dubeikovskaya Z.A., Trofimenko A.F., Aslanyan E.M., Kachaturian S.V., Dobrica A.P. Gene expression of delta endotoxin of B.thuringiensis var. berliner in E.coli and B .megatherium cells. Mol. Gen. Microbiol. Virol., 1990, 6, 24-28..

6.Dubeikovskaya Z.A., Franc R.I., Pahtuev A.I., Shevcov V.V. Evaluation of delta endotoxin of B. thuringiensis by immunochemical methods. Biotechnology, 1990, 5, 89-92.

5.Dubeikovskaya Z.A, Shachanina K.L., Moroz A.F., Alexandrov A.D., Alexandrova I.A. Determination of antibodies of Pseudomonas aeruginosa in serum. In: ELISA in biology and medicine. Moscow, 1983, 94-98.

4.Dubeikovskaya Z.A., Savitskaia K.I., Anciferova N.G., Shashanina K.L., Moroz A. F. Enzyme-linked immunosorbent assay for detection specific antibodies of Pseudomonas aeruginosa. Journal of Microbiol. Epidemiol. Immunol., 1983, 8, 53-57.

3.Edvabnaya L.S., Shahanina K.L., Dubeikovskaya Z.A., Palkina N.A. dentification lipopolisaccharide of Pseudomonas aeruginosa by ELISA. Journal of Microbiol. Epidemiol. Immunol., 1983, 1, 36-39.

2.Shahanina K.L., Moroz A.F., Dubeikovskaya Z.A., Anciferova N.G., Savitskaia K.I. “Determination of specific antibodies against slime of Pseudomonas aeruginosa in the serum of patients with acute and chronic deseases of lungs by ELISA”. In: “Infections caused by opportunistic bacteria- Staphilococcus and Pseudomonas aeruginosa”, Moscow, 1982, 48-52 (Russ).

1.Dubeikovskaya Z.A., Anciferova N.G., Shahanina K.L., Moroz A.F. Serological studies of the slime components of Pseudomonas aeruginosa. In: “Infections caused by opportunistic bacteria- Staphilococcus and Pseudomonas aeruginosa”, Moscow, 1982, 40-47 (Russ).


Dr Timothy Wang,

Dorothy L. and Daniel H. Silberberg Professor of Medicine,

Chief of Division of Digestive and Liver Diseases, Department of Medicine, Columbia University, New York, US


Phone: 212-***-****

Dr Tatiana Kisseleva, Adjunct Professor of Surgery, Department of Surgery, University of California, San Diego, US


Phone : 858-***-**** (lab)

858-***-**** (do not have a voice mail)

Dr Daniel Worthley, Head of Gastrointestinal Cancer Biology Group, The University of Adelaide, Australia


Phone: 61 4 0036 3208

Dr Michael Quante, Principal Investigator, Technical University of Munich, Germany


Phone: 49-89-414*-****

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