Professional Experience Manager

Sajan Shrestha

**-** ****** *** *********, New York, 11385

Tel: 929-***-**** E-mail: ***********@*****.*** Fully Work Authorized. No Visa Sponsorship Required. Professional summary

Biologist with more than 3 years of research experience in field biology, lab techniques and performing chemical experiments.

Key skills

• Detailed study of the fundamentals of Good Manufacturing Practice (GMP) such as hygiene, pest control, sanitation and waste disposal.

• Knowledge of Hazard Analysis Critical Control Point (HACCP) and the development and implementation of Standard Operating Procedure (SOP)

• Experienced in ELISA testing, PCR, RDT, Micrometry and Culture and Identification of micro- organisms

• Prepared buffers, standard solution and sample solution and Performed titration, dilution, filtration, extraction and calibration

• Experienced in using, fixing and maintenance of instruments such as: pH meter, microscope and centrifuges

• Theoretical knowledge of: HPLC, GC, Spectrophotometer, IR, UV

• Theoretical knowledge of: Southern blotting, Northern blotting, Western blotting, embryology and cell biology

• Sterilization and Disinfection techniques

• Worked with hazardous materials and chemicals and neutralized waste products

• Computer (Word, Excel, Power Point, Outlook)

• Fluent in English, Hindi and Nepali

Professional experience

Lecturer

Times college, Kathmandu, Nepal 20/201*-**-****

• Planed, delivered lectures and supervised to undergraduate students at biological laboratory

• Checked practical notes of students, find out the problems and solved them Researcher (Parasitology)

Tribhuwan University, Nepal 06/2013-01/2015

• To find out the parasitic infection and cross-transmission between wild (Red Pandas) and domestic animals (goats, sheep, cows, buffaloes, horse), samples were collected from field and preserved in 5% Potassium dichromate

• Collected samples were centrifuged, prepared slides by staining with iodine solution and methylene blue and examined in microscope

• Recorded data was analyses by SPSS and results showed that 100% of the animals were infected with either protozoan or helminth parasites and transmission of parasites between wild and domestic animals were possible at the study area

Laboratory demonstrator in biology lab. 01/2013-06/2013 Trichandra multiple college

• Operated and maintained laboratory equipment including centrifuge, microscope and glassware and Performed techniques such as: titration, dilution, filtration, distillation and extraction

• Prepared solutions such as: Formalin, Iodine, Methylene blue to fixed and stain the parasites

• Cultured of bacteria and protozoan to find out the growth rate and identification and Prepared thick and thin blood smear to examined the malaria parasite (Plasmodium) and microfilaria

(Wucheria bancrofti)

• Performed Enzyme Linked Immune Sorbent Assay (ELISA) to find out the sample was exposed to virus (antigen) or other substances that may cause chronic infection

• Performed Polymerase Chain Reaction (PCR) to amplify a single or few copies of a DNA into many copies of particular DNA sequence

• Sterilized and disinfected the equipment including slides, glassware and strainer Professional development

Participated in Nepal Academy of Science and Technology (NAST) conference Kathmandu, Nepal, March 29-31, 2016

• Presented paper(Oral) in title “Cross-transmission of gastrointestinal parasites between Red Panda and Domestic animals (Goat, Sheep, Cow, Buffalo, Horse).” Participated in Himalayan Knowledge Conclave Kathmandu, Nepal, April 4 and 5, 2016

• Presented paper(Poster) in title “Parasitic burden in Red Panda”. Education

Master of science in Zoology. 06/2011-05/2013

Tribhuwan University, Kathmandu, Nepal

Thesis: Cross transmission of gastrointestinal parasites between wild animal (Red Panda) and domestic animals (Cow, Buffalo, Goat, Sheep, Horse).

Course of interest: Molecular biology, Cell biology, Pharmacology, Oncology, Anatomy, Embryology, Epidemiology, Pathology, Microbiology, Methodology, Pest and Pesticides, Parasitology, Genetic biology, Ecology, Protein synthesis

Bachelor of science (Zoology, Botany, Chemistry). 04/2008-01/2011 Trichandra Multiple College, Kathmandu, Nepal

Report: Butterfly fauna in Shivapuri National Park, Kathmandu, Nepal. Publication

Sajan Shrestha and Mahendra Maharjan “Parasitic burden in Red panda (Ailirus fulgens Cuvier, 1825) of Ilam district Community forest, Nepal.” Nepalese Journal of Zoology, Nov. 2015

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