Medical Research
Resume:
Scott A. Helms
Conway, AR *****
Ph: 501-***-****
Email: *****.******@*****.***
KEY ACHIEVEMENTS
Developed referenced methods for quantitative analysis of histology images. Developed a method of biomarker selection based on preservation of ordinality across an ensemble of projective methods based on non-transformable pre-filtered sets.
EDUCATION
August 2005 – May 2011: University of Arkansas for Medical Sciences: PhD
August 2001 – May 2005: University of Arkansas at Little Rock: BA in Chemistry, Minor in Biology
August 1988 – July 1995: Arkansas State University: Concentration in Biology and Manufacturing
Technology
NON-RESEARCH LABORATORY EXPERIENCE
Laboratory Technician
August 2016 – April 2017
Toxicology Department, Pathology Partners LLC
Supervisor: Chenbo Dong, PhD
● Preparation of patient urine samples for LC-MS for determination of prescription drug compliance. This included the use of internal standards, external quality controls, and concurrent calibration curves, enzymatic digestion, dehydration, and solid phase extraction.
● Development of system for the prediction of preparation run completion time, and occasional setup of LC-MS runs.
RESEARCH EXPERIENCE
Post-Doctoral Fellowship
April 2012 – June 2014
Department of Environmental and Occupational Health
Fay W. Boozman College of Public Health
University of Arkansas for Medical Sciences
Principal Investigator: Gunnar Boysen, PhD
● Untargeted analysis of mass spectrometry (TOF) data for the distinction of cell line and lung tumor types obtained by biopsy.
● Development of robust a analytical method for the selection of feature sets capable of separating groups, based on lack of variability in rank across analytical models.
Graduate Research
May 2006 – May 2011
Donald W. Reynolds Center on Aging
University of Arkansas for Medical Sciences
Advisor: Jeanne Y. Wei, MD, PhD, Department of Geriatrics, UAMS
● Fluorescence microscopy and quantitative image analysis to determine fractional localization of proteins in transfected cells.
● Use of online tools to identify nuclear localization signals in target proteins.
● Use of the PIXImus system (x-ray densitometry) to determine the lengths of mouse tibia.
● Sectioning and histological (H&E, Masson Trichrome) staining of mouse hearts.
● Measurement of nuclear size, cell size, sarcomere length, and collagen area using ImageJ.
● Analysis of promoter regions of genes and miRNA’s found to be up- or down-regulated in the presence of enhanced levels of SRF for the CArG box motif.
● In-silico analysis of 10Kbp promoter regions and first and second introns for interactions of locations and orientations of binding sites for SRF, ETS, ATF, and NFAT with regard to their combinatorial effects on gene expression.
● Additionally, I was trained in Western blot, rt-PCR, and mouse colony management.
Third Graduate Research Rotation
March 2006 – May 2006
Donald W. Reynolds Center on Aging
University of Arkansas for Medical Sciences
Advisor: Jeanne Wei, MD, PhD, Department of Geriatrics, UAMS
● Analysis of microarray data for selection of genes up- or down-regulated in the presence of excess SRF.
Second Graduate Research Rotation
December 2005 – March 2006
John L. McClellan Memorial Veterans Hospital
Little Rock Arkansas
Advisor: Mark D. Crew, PhD, Department of Microbiology and Immunology, UAMS
● Isolation, separation, and semi-quantitation of select DNA from bacterial culture using agarose gel electrophoresis, PCR, and phosphor imaging.
First Graduate Research Rotation
August 2005 – December 2005
Advisor: Anna Radominska-Pandya, PhD, Department of Biochemistry, UAMS
● Enzyme kinetics of UDP-glucoronidase variants versus several substrates by digestion, thin- layer chromatography, autoradiography, and scintillation counting.
Undergraduate Research: Modeling of Oxidized Fatty Acids
January 2002 – December 2004
Advisor: Robert T. Swindell, PhD, Department of Chemistry, UALR
● Computational chemistry Newtonian and ab-initio modeling of interactions of sphingomyelin with cholesterol.
RESEARCH INTERESTS
● Network statistics built on geometric calculus.
● Autonomous agents.
AWARDS and HONORS
● UALR Chemistry Department: Outstanding Graduating Senior Award (2005)
● UALR Physics Department: Elementary Physics Student of the Year (2003)
PUBLICATIONS
Manuscripts
Sappington DR, Helms SA, Siegel ER, Penney RB, Jeffus S, Bartter T, Bartter T, Boysen G. (2017) Diagnosis of lung tumor types based on metabolomic profiles in lymph node aspirates. Canc Treat & Res Comm. 14 (2018) 1–6
Zhang X, Azhar G, Helms S, Burton B, Huang C, Zhong Y, Gu X, Fang H, Tong W, Wei JY. (2011) Identification of New SRF Binding Sites in Genes Modulated by SRF Over-Expression in Mouse Hearts. Gene Regul Syst Bio. 5:41-59.
Zhang X, Azhar G, Helms SA, Wei JY. (2011) Regulation of cardiac microRNAs by serum response factor. J Biomed Sci. 18:15.
Helms SA, Azhar G, Zuo C, Theus SA, Bartke A, Wei JY. (2010) Smaller cardiac cell size and reduced extra-cellular collagen might be beneficial for hearts of Ames dwarf mice. Int J Biol Sci. 6(5):475-90.
Zhang X, Azhar G, Helms SA, Zhong Y, Wei JY. (2008) Identification of a subunit of NADH-dehydrogenase as a p49/STRAP-binding protein. BMC Cell Biol. 9:8.
Manuscripts in Preparation
Helms SA, Nookaew I, Siegel ER, Sappington DR, Boysen G. Effects of data analysis variables on feature section form metabolomic data reveal the advantage of consensus features for group separation over individual data analysis approaches.
Dissertation
Scott Allen Helms. (2011). Modulation of Gene Expression by SRF in Mouse Hearts. University of Arkansas for Medical Sciences. Little Rock, Arkansas. 164pp.
Posters and Abstracts
Daniel R. Sappington, Scott Helms, Ishwori B. Dhakal, Eric R. Siegel, Thaddeus Bartter, Gunnar Boysen. Molecular characterization of lung tumors based on metabolomic profiling. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 3236. doi:10.1158/1538-7445.AM2013-3236
62nd Gerontological Society of America, Annual Scientific Meeting Atlanta, Georgia. November 21, 2009 BS Poster Session III, 8:00 AM to 6:00 PM. (Poster) Zhang X, Helms SA, Azhar, Wei JY. Not All Genes with Classic CArG Elements are Differentially Regulated by SRF in Mouse Hearts.
62nd Gerontological Society of America, Annual Scientific Meeting Atlanta, Georgia. November 21, 2009 BS Poster Session III, 8:00 AM to 6:00 PM, (Poster) Helms SA, Azhar G, Zuo C, Theus S, Bartke A, Wei JY. Smaller Cardiomyocyte Size and Reduced Collagen Confers Longevity Benefit in the Hearts of Ames Dwarf Mice.
227th ACS National Meeting (Anaheim)/CHED
Undergraduate Research Poster Session: Biochemistry
Mon 03/29/2004 11:00 AM Convention Center Hall A, Paper 259: (Poster) The effect of epoxidation of palmitoylsphingomyelin on interactions with cholesterol. S. Helms, R. Swindell
225th ACS National Meeting (New Orleans)/CHED
Undergraduate Research Poster Session: Biochemistry
Mon 03/24/2003 2:00 PM Convention Center Hall J, Paper 924: (Poster) Molecular modeling of unsaturated fatty acids and fatty acid epoxides. L. Coney, R. Jenkins, S. Helms, R. Swindell
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