Neha Ghosh, PhD

Walnut Creek, CA *****

785-***-**** ac1z3q@r.postjobfree.com

Scientist & Laboratory Manager

Distinguished Scientist with remarkable success working in a highly matrixed environment to drive scientific and technical research and innovation, disseminate data, and present findings. Track record of productivity, effective verbal, written and interpersonal skills has resulted in the following:

Presenting before senior stakeholders, doctors, and clients on assays and releasing patents.

Participating in multidisciplinary efforts to plan experiments in support of development and validation of new reagents, assays, and instrumentation for protein-based research.

Interacting with other R&D teams to assess feasibility of new product concepts.

Handling multiple projects and priotities while liasing between staff to chart /report data.

Guiding and supervising Research Scientists, Post-Docs, PhDs and Master students in writing and developing project proposals, dissertations, reports, SOPs, assays, and lab safety protocol.

Efforts completing analytical and process development in timely fashion with scientific rigor.

Education

Defence Research and Development Establishment (DRDE), Gwalior, India

Dissertation: “Development of immunological detection and diagnostic systems for anthrax from environmental and clinical samples”

Ph.d. in Biological Sciences

Jiwaji University, Gwalior, India

Thesis: “Study of protein expression pattern following exposure to Polycyclic Aromatic Hydrocarbons (PAHs) using Proteomic approach”

Master of Science in Biotechnology

Bachelor of Science in Biotechnology

Professional Experience

Trinity Biotech, Kansas City, MO

Scientist, 2015-Present

Collaborate with staff in a fast-paced environment with a high degree of autonomy to design experiments, interpret data, troubleshoot technical problems, and establish new analytical assays for the quantitation of diabetic biomarkers (HbA1c).Conduct in-depth studies and evaluate test results to develop innovative solutions for improvements of Premier Hb9210 consumable products. Adhere to Food and Drug Administration (FDA) processes and general laboratory procedures for FDA 510(k) approvals.

Develop strategic reports and present findings on software validation testing programs.

Regulate HPLC and market or validate consumable products from GMP and cGMP laboratories.

Build and maintain customer relations through structured training seminars and presentations.

Coordinate large-scale data analysis using Microsoft Office Excel, Word, PowerPoint, Outlook and graphic editing software implement HPLC analytical methods for HbA1c testing.

King Abdullah University of Science and Technology (KAUST), Saudi Arabia

Lab Manager; Lab Safety Officer, 2014-2015

Spearheaded developments and maintained the Epiginetics lab: Developed key safety protocols, SOPs, and decisions to advance lab safety and administration. Supervised and trained PhD students and researchers on troubleshooting and designing SOPs. Met and collaborated with research groups to exchange ideas and determine best courses of action in and outside of the laboratory.

Plan and conduct experiments and troubleshoot issues to support new product development.

Acted as the main point of contact on laboratory procedures: liased between staff and departments to coordinate research developments and determine next steps.

Wrote and reviewed technical reports, method transfer protocols and present and interpret data at project team meetings.

Developed and finalized new operating and lab safety protocols in a competitive lab.

Participated in lab research, seminars, and experiments to further scientific discoveries.

Devised an inventory on reagents allocations produced or stored in lab compartment built a functional “Historical Memory” to advance scientific research and discovery.

Exercised good judgment on a team while retaining the ability to work independently and multitask on projects. Administered MTAs, shipments, and process development.

Compile data and prepare technical reports for presentation to project and management teams.

Liase between Engineering, Marketing, and Sales teams to improve information transfer.

Defense Research and Development Establishment (DRDE), India

Senior Research Fellow, 2009-2014

Developed immunological detection systems for serodiagnosis of anthrax in humans and animals. Performed cloning, production and purification of recombinant proteins and determined their utilization in development of immunoassays for diagnosis of anthrax. Plan and implement laboratory procedures and computerized data handling

Multitasked on project development while maintaining/demonstrating initiative, creativity, and tangible productivity. Key project developments included the following:

An anti-Protective Antigen IgG Enzyme-linked immunosorbent assay (ELISA) for diagnosis of cutaneous anthrax in India.

A field usable qualitative anti-PA enzyme linked immunosorbent assay(ELISA) for serodiagnosis of human anthrax..

A quantitative Anti-Protective Antigen IgG Enzyme-linked immunosorbent assay (ELISA) for the quantification of anti-PA IgG in human sera.

An anti-Lethal Factor IgG Enzyme-linked immunosorbent assay (ELISA) for serodiagnosis of human cutaneous anthrax in India.

A Surface Plasmon Resonance (SPR) Biosensor based Detection of Bacillus anthracis, from human serum samples targeting Protective Antigen.

A universal Flow through system (Point of care ELISA) for serodiagnosis of anthrax infection in human and animals.

A sandwich ELISA system for the detection and confirmation of Bacillus anthracis spores in artificially spiked samples.

A Surface Plasmon Resonance (SPR) Biosensor based detection of Bacillus anthracis, from Soil Samples Targeting Protective Antigen.

A simple and rapid on-spot membrane ELISA for detection and differentiation B. anthracis, the causative agent of anthrax.

Additional Experience

Jiwaji University, Gwalior, India

Research Fellow: Preceptor: Prof. GBKS Prasad, PhD, 2008-2009

Researched and identified molecular targets of polyherbal formulation for Type II diabetes.

Birla Insititute of Medical Research (BIMR), Gwalior, India

Ad-Hoc Lecturer, 2007-2007

Instructed graduate-level biotechnology courses and supervised complex laboratory experiments.

Technical Skills

Biochemistry, Cellular and Molecular Biological Methods:

Genome editing (using CRISPR/Cas9), Cloning, expression and purification of proteins, DNA Transformation in Prokaryotes and Eukaryotes, Real Time PCR, RT-PCR, qPCR, Standard PCR, Nested PCR, 2D-Gel electrophoresis, Plasmid isolation, DNA isolation, RAPD, RFLP,RNA Isolation, Proteomics, Genomics and fingerprinting procedures.

Immunological Techniques:

ELISA, Cytokine assay, Neutralization assay, Immunofluorescence, Immunoblotting, Production and purification of polyclonal and monoclonal antibodies, Immunochromatography, Multibead Assay, Nano ELISA, Immunosensors, Enzymatic assay development, Antigen detection kit development, Antibody detection kit development, assay development, quatification and analysis of samples, broad knowledge of working with blood, serum/plasma.

Analytical Instruments:

Phase Contrast Microscope, Fluorescence Microscope, Bright Field Microscope, HPLC, LC/MS, Ion exchange chromatography, Affinity chromatography, Gel Filtration chromatography, MALDI-TOF-TOF and Genomics and Proteomics tools, Surface Plasmon Resonance, AKTA Purification System, SDS PAGE, Gel Elcetrophoresis, Western Blot

Analysis tools:

GraphPad Prism, SigmaStat, Microsoft Office Suite: Excel, BLAST, ClustalW,tree view, MLST.

Handling of Pathogenic Microbes:

Highly skilled in handling extremely pathogenic bacteria, including Category A Biowarfare agents in BSL-3 and BSL-2 containment.

Animal Handling:

High expertise in handling small laboratory animals and working with rats, mice and rabbits. Knowledgeable of immunization and blood collection through different routes (intraperitonial, oral, sub-cutaneous, intradermal and intramuscular) and dissection of small laboratory animals.

Research & Projects

Trinity Biotech, Kansas City, MO

Scientist, 2015-Present

Validation of PremierHb9210 Software ver 9.1.1.17 for market release.

Research data analysis for identification and study of buffer bias with PremierHb9210.

Ruggedness and efficacy study of Premier Hb9210 consumables and columns.

Writing validation protocols for FDA 510(k) claims for Premier Hb9210 as the detection system for Diabetes biomarker (HbA1c).

King Abdullah Univesrity of Science and Technology (KAUST), Saudi Arabia

Lab Manager; Lab Safety Officer, 2014-2015

Establishment of new epigenetics lab and laboratory protocol and safety procedures.

Carry out routine inspection of laboratory intruments including safety equipments.

Defense Research and Development Establishment (DRDE), India

Senior Research Fellow, 2009-2014

Cloning, Expression, and purification of recombinant proteins in suitable host vector.

Production and purification of antibodies for differents antigens in Rabbit and mice.

Development of immunodiagnostic assays for detection of anthrax.

Development of molecular diagnostic assays for detection of anthrax.

Exoproteome analysis of novel strains of bacteria implicated in disease resembling anthrax.

Differentiation of bacterial species in anthrax like infections in humans and animals.

Honors & Awards

M.P Council of Science and Technology, India

27th M.P Council of Science and Technology Young Scientist Award: Best Presentation, 2012

Indian Council of Medical Research(ICMR), India

Senior Research Fellowship (SRF) Award, 2012

Defence Research and Development Establishment (DRDE), India

(Collaborative Project with Jiwaji University, Gwalior,India)

Senior Research Fellowship (SRF) Award, 2011

M.P Council of Science and Technology, India

Junior Research Fellowship (JRF) Award, 2008

Jiwaji University, Gwalior,India

Silver Medal in M.Sc Biotechnology: Academic Outstanding Performance, 2007

Jiwaji University, Gwalior,India

Special Appreciation Award for Outstanding Student of BIMR College of Life Sciences, 2006

Bharat Vikas Parishad, Gwalior, India

Special Appreciation Award for Outstanding Performance in Academics. 2005

Patents

1.Method and kit for analyzing the presence of Bacillus anthracis. Neha Ghosh and A. K. Goel Indian Paten Filed No. 853/MUM/2012. (TOT In-Process)

2.A broth medium for growth of Bacillus anthracis, a media to analyze its presence in a sample for differentiation, and a kit for conducting such analysis. A. K. Goel and Neha Ghosh. Indian Patent Filed No. 1087/MUM/2012. (TOT In-Process)

Presentations

1.Ghosh, N., Gupta, N., Tomar, I., Goel, A.K.. A direct immunofluorescence assay for rapid and specific detection of Bacillus anthracis spores from the environmental samples. 53rd International Conference of Microbiologist of India. Bhubaneswar, India. 2012.

2.Ghosh, N., Tomar, I. and Goel, A.K.. A field usable qualitative anti-protective antigen (PA) enzyme linked immunosorbent assay for serodiagnosis of human anthrax. 53rd International Conference of Microbiologist of India. Bhubaneswar, India. 2012.

3.Ghosh, N. Isolation and characterization of a Bacillus cereus strain having the pathogenic potential of Bacillus anthracis associated with illness resembling cutaneous anthrax in South India. 27th M.P Young Scientist Congress, Bhopal, India, 2012.

4. Ghosh, N., Alam, S.I, Goel, A.K.. Bacillus cereus strain associated with illness resembling cutaneous anthrax in South India. 15th International Congress on Infectious Diseases. Bangkok, Thialand. 2012

5.Ghosh, N. On-Site Label Free Ultra Sensitive Detection of Bacillus anthracis Directly from the Environmental Samples Using Surface Plasmon Resonance. 26th M.P Young Scientist Congress, Jabalpur, India, 2011.

6.Ghosh, N., Kumar, P., Goel, A.K., Bhattacharya, P., Kamboj, D.V., Kumar, O., Padmaja, J. and Rao, T. N. Multidrug resistant Pseudomonas species isolated from skin lesions of patients from a tribal area in India. ICAR, Spain, 2010.

7.Ghosh, N., Goel, A.K. and Kamboj, D.V.. Direct detection of Bacillus anthracis in animals using Polymerase Chain Reaction. Indo-US workshop on Chemical and Biological Defence, Gwalior, India, January 21-22, 2010.

8.Ghosh, N., Kumar, P., Goel, A. K. and Kamboj, D.V. Environmental detection of Bacillus anthracis using Polymerase Chain Reaction. 50th Annual Conference of AMI, Pune, India, December 15 – 18, 2009.

9.Gupta, G.,, Ghosh, N.,, Boopathi, M., Goel, A.K., Singh, B., Vijayaraghavan, R. Rapid detection of biological warfare agent Bacillus anthracis by surface plasmon resonance. International Symposium on recent advances in ecology and management of vectors and vector born diseases. Defence Research and Development Establishment, Gwalior, India. 2010.

10.Jain, M., Kumar, P., Ghosh, N., Goel, A.K., Kamboj, D.V. Molecular epidemiology of cholera outbreak in India reveals continuous evolution of new variants of Vibrio cholera O1. Molecular Epidemeology and Evolutionary Genetics of Infectious Diseases Conference, Amsterdam, Netherland, November 3-5, 2010.

11.Yadav, D., Anand, A., Zacharia, A., Ghosh, N., Bhadouria, P., Bisen, P.S., Prasad, G.B.K.S. The emerging link between metabolic syndrome and diabetes in Gwalior-Chambal region of Central India on the basis of the clinical implications. 3rd International Congress on Prediabetes and the Metabolic Syndrome, Nice, France, April 1-4, 2009.

12.Prasad, G.B.K.S., Pathak, V., Yadav, D., Singh, N., Dubey, S., Ghosh, N., Bisen, P.S. Role of polyherbal preparations in management of oxidative stress in diabetes mellitus. International Conference on Molecular Mechanism of Diseases, DRDE,Gwalior,December 15-16, 2008

13.Yadav, D., Shrivastava, P., Ghosh, N., Bind, A., Prasad, G.B.K.S. Tolerance to honey by subjects with parental history of diabetes. International Conference on Molecular Mechanism of Diseases, DRDE, Gwalior, December 15-16, 2008.

Publications

Peer-reviewed Journal Articles:

1.Goel, A.K., Ghosh, N., Puranik,N., Varshney, A., Kumar, M.. 2016 Development of a rapid point of care immuno-filtration assay for serodiagnosis of cutaneous anthrax in India. International Journal of Infectious Diseases. 45: 151

2.Ali Z., Abul-faraj A., Li L., Ghosh N., Piatek M., Mahjoub A., Aouida M., Piatek A., Baltes N.J., Voytas D.F., Dinesh-Kumar S., and Mahfouz M.M. 2015. Efficient Virus-Mediated Genome Editing in Plants using the CRISPR/Cas9 System. Molecular Plant. 8: 1288-1291.

3.Ghosh, N., Gunti, D., Lukka, H., Reddy, B.R., Padmaja, J., Goel, A.K. A quantitative enzyme linked immunosorbent assay for measurement of anti-protective antigen IgG in human cutaneous anthrax cases. 2015. Indian Journal of Medical Research. 142: 103-111

4.Ghosh, N., A.K. Goel and S.I Alam. Exoproteome analysis of a novel strain of Bacillus cereus implicated in disease resembling cutaneous anthrax. 2014. Infection, Genetics and Evolution 22: 1–11.

5.Ghosh, N., N. Gupta,G. Gupta, M. Boopathi, V. Pal, A.K. Goel. 2013. Detection of protective antigen, an anthrax specific toxin in human serum by using surface plasmon resonance. Diagnostic Microbiology and Infectious Disease. 77 :14–19.

6.Ghosh, N., Tomar, I. and Goel, A.K. 2013. A field usable qualitative anti-PA enzyme linked immunosorbent assay for serodiagnosis of human anthrax. Microbiol Immunol. 57:145-149.

7.Ghosh, N., Tomar, I., Lukka, H. and Goel, A.K. 2013. Serodiagnosis of human cutaneous anthrax in India using an indirect anti-lethal factor IgG ELISA. Clin Vacc Immunol. 20: 282-286.

8.Ghosh, N. Gupta, G., Boopathi, M., Singh, A.K., Gopalan, N., Pal, V., Goel, A.K.. 2013. Surface Plasmon resonance based detection of Bacillus anthracis in soil samples using monoclonal antibodies. Indian J. Microbiol. 53(1):48-55.

9.Ghosh, N. and Goel, A.K. 2012. An anti-protective antigen IgG enzyme-linked immunosorbent assay for diagnosis of cutaneous anthrax in India. Clin Vacc. Immunol. 19: 1238-1242.

10.Ghosh, N., Alam, S., Goel, A.K. 2012. Prevalence of Bacillus cereus strains associated with illness resembling cutaneous anthrax in South India. Int J Infect Dis. 40(6):570-71.

11.Ghosh, N., Goel, A.K., Bhattacharya, P., Lukka, H.V., Kamboj, D.V. 2011. Prevalence of drug resistant and exotoxin A producing Pseudomonas aeruginosa in cutaneous infections in a tribal area in South India. African J Microbiol Res. 5(30): 5512-5517.

12.Goel, A.K., Jain, M., Kumar, P., Sarguna Meera Bai, P., Ghosh, N., and Gopalan, N. 2011. Molecular Characterization Reveals Involvement of Altered El Tor Biotype Vibrio cholerae O1 Strains in Cholera Outbreak at Hyderabad, India. Journal of Microbiology. 49(2): 280-4.4.

Journal Articles In-Progress:

1.Ghosh, N., A. K Goel and S. I Alam. Molecular characterization of B. cereus strains having pathogenic potential similar to Bacillus anthracis and causing symptoms resembling cutaneous anthrax in India. (Article under preperation).

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