Manufacturing Research
Resume:
Sumathi Jayakumar, M.Phil, ASCP (MB)
Dublin, CA *4568
*******.*.*********@*****.***
Summary of Qualifications
Senior level molecular biologist experienced in research and manufacturing environments.
Proficient in a variety of molecular biology techniques – Small and large volume Nucleic acid extraction (Miniprep), DNA quantification (Bioanalyzer, Nanodrop), NGS sample preparation (Ion Torrent PGM), PCR/qPCR and RNA isolation from tissue samples.
Experienced with liquid handlers, automated systems, data analysis (Excel) and ELN. Fast learner and good attention to detail.
Proficient in manufacturing practices – cGMP, cGLP.
Proficient in handling samples from various tissues.
Ability to handle large volumes of biological specimens. Experienced in handling 300+ BAC libraries and 200,000+ clones.
Highly organized with excellent communication skills and a proven ability to multi-task.
ASCP certification in Molecular Biology, licensed as a CGMBS Trainee
Experience
Associate Scientist, Merck Research Labs. May 2017 – Aug 2017
RNA isolation using Stat 60 method from various tissue samples e.g. spleen, lymph node, small intestine, colon, blood
Quality analysis of RNA using Agilent Bioanalyzer
Document projects using an Electronic Lab Notebook (ELN)
Manufacturing Technician, Linkage Biosciences. Sep 2015 – May 2017
Design, execute and optimize assays using data analysis software
Produce HLA typing kits using ABI 7900, QuantStudioTM 6 and Roche platforms
Perform and troubleshoot layout, buffer, plate and sample QC experiments using PCR/qPCR
Follow ISO compliant work instructions and Standard Operating Procedures (SOPs). Maintain detailed records and documentation (batch and production records) as per Current Good Manufacturing Practices and Current Good Laboratory Practices (cGMP, cGLP)
Mentoring and training junior associates on various aspects of the manufacturing process
Associate, CSU East Bay. Jan 2012 – May 2012
Prepare samples using Ion Torrent Fragment Library Kit for Next Generation Sequencing (NGS).
Perform end-repair of DNA, ligation using adaptors, size selection using Pippin Prep and library QC using Bioanalyzer.
Good knowledge of Cytomegalovirus from Microbiology and Advanced Molecular Techniques course work.
Staff Research Associate, Children’s Hospital Oakland Research Institute. Aug 2001 – Sep 2011
Constructed and organized genomic libraries and individual clones for further research. The Bacterial Artificial Chromosome (BAC) libraries were used for human-genome sequencing and for the creation of knockout mouse models. Completed annual bio-safety training and performed all procedures in compliance with company’s bio-safety protocols
Job functions included the following:
Responsible for handling orders for complete genomic libraries and individual clones and for fulfilling and shipping those orders. Organized more than 300 genomic libraries and more than 200,000 clones belonging to various species and diseases.
Library Screening. Performed hybridization using radio labeled synthetic Oligonucleotide probes.
Quality-Assurance of Knockout Mouse Models. Prepared samples for Roche 454 sequencer, Performed Gateway reactions, PCR for further research.
Standardize Fosmid Library Pool Strategies. Standardization of pool strategies to construct Pine Fosmid libraries. Performed serial dilution of the harvested cells to determine optimal pool size.
Single colony isolation of BAC clones. Responsible for accurately streaking and picking specific clones from various libraries (200 clones/day).
Characterization of BAC-clones. Performed Pulse Field Gel Electrophoresis (PFGE) of digested BAC DNA to study average DNA insert size.
BAC Library Production. Responsible for isolating BAC clones using Q-Pix Robots (Cherry picker) into 384-well format micro titer plates.
Re-arraying BAC-clones from 384 to 96-well format. Programmed and operated Q-Select robots (600 clones/day).
BAC-clone Replication. Programmed and operated Q-Select robot to replicate BAC and Fosmid libraries into 384 well plates.
Macro array (Gridding). Generated macro arrays of BAC and Fosmid libraries using BioGrid robots. The macro arrays were used for library screening, gene expression and gene discovery.
Techniques: Hybridization using P32 radioactive probes, clone analysis, PCR (small volume, less than 10μl and multichannel pipetting), Southern blot, Pulse Field Gel Electrophoresis (PFGE), DNA sample preparation, single colony isolation of BAC clones, transformation of cells using electroporation techniques, Programming Q-Pix, Q-Select, and BioGRID robots (Liquid handling), Nylon membrane filters, spreading and harvesting cells.
Skills
Molecular Biology
Extensive experience in DNA isolation and analysis using hybridization techniques.
Extensive experience in PCR (small volume, less than 10μl and multichannel pipetting) standard and pulsed field Gel Electrophoresis, molecular assays, gateway reactions, and plasmid miniprep.
Experienced in Ion Torrent NGS Library Kit, Bioanalyzer, qPCR, Qubit, RNA isolation from various tissues
Computer Skills
Proficient in a range of software applications including Microsoft Word, Microsoft Excel and ELN.
Well versed in liquid handling systems (Hamilton, Integra, BioGRID, etc.)
Education
CSU East Bay, Hayward, CA
Statistical methods – Descriptive and inferential statistics including hypothesis testing using ANOVA and Chi Square.
Introduction to Hematology (Undergraduate Level).
Advanced Molecular Techniques. Isolation and characterization of ORFeome clone and site-directed mutagenesis (SDM). (Graduate Level)
Functional Genomics. Isolation and characterization of miRNA from White Lupin growing in Phosphorous deficient conditions. (Graduate Level)
2014-2015 Coursera
Bioinformatics I including BLAST, ClustalW, MSA, Phylogenetics, Functional Genomics and Precision Medicine
1994–1995 Anna University, Chennai, India
Master of Philosophy with dissertation in Biochemistry
1989–1994 Madras University, Chennai, India
B.Sc. & M.Sc. in Biochemistry
Master of Philosophy Dissertation
Biochemical Effects of Tefroli on Carbon Tetra Chloride induced Rats
This project was part of my Master of Philosophy thesis. As part of this project, I studied the peroxidation and accumulation of lipids in CCl4 induced rats. The induced rats developed liver necrosis. The disease was cured using Tefroli a commercially available herbal drug. The results showed that Tefroli could be an effective hepato-protective agent.
Techniques used. Animal handling, biochemical assays and data analysis.
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