Phillip A. Harding

** ******* **, ******, ** ****1

ac1sxb@r.postjobfree.com • 724-***-**** • www.linkedin.com/in/pahardingr

Education

The Johns Hopkins University Baltimore, MD

Bachelors of Science, Biomedical Engineering Graduation 2016

Experience

Dr. Stephen J. Gould Research Laboratory October 2016 – Present

Research Technician

Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, MD

Overall projects aim to understand the mechanism of exosome biogenesis for future use as conduits for DNA and RNA delivery, as well as small molecules

Worked closely with Principal Investigator and PhD students to conduct experiments based on gene knockouts of exosome-budding proteins in human embryonic kidney cells (CD63, CD9, CD81, ALIX, Syntenin)

Independently studied the effects of protein kinase C activators and inhibitors on the production of exosomal protein markers

oAnalyzed the relative abundance of proxy markers using gel electrophoresis and western blot techniques

Coordinated the daily workings of the laboratory, including inventory and budget

Build-a-Genome, Synthetic Biology Research Group January 2016 – May 2016

Undergraduate Research Assistant

Department of Biomedical Engineering, The Johns Hopkins University, Baltimore, MD

Project aimed at designing, constructing, and integrating an entirely synthetic version of the yeast Saccharomyces cerevisiae genome

Used PCR amplification to create synthetic DNA segments that were transformed into bacteria and yeast plasmids to test accuracy of synthetic segment

Novel SCRaMbLE technique was used to generate phenotypically diverse yeast

oSCRaMbLE-WT diploids were created to test viability of specific genotypes and phenotype

Learned skills: PCR amplification, gel electrophoresis, bacterial and yeast transformation, and DNA cloning and sequencing (Sanger)

Translational Tissue Engineering Center April 2014 – November 2014

Undergraduate Research Assistant

Department of Biomedical Engineering, The Johns Hopkins University, Baltimore, MD

Project aimed to use infrared-activated gold nanoparticle-infused biomaterials as a novel drug delivery method

Developed branched gold nano-particles for incorporation into PBAE hydrogels

Determined a polymer ratio that would allow for the correct physical attributes necessary for drug delivery

Conducted experiments to test the release of doxrubicin from hydrogel by mass differences after incubation

Skills & Activities

Programming: MATLAB, Python, R

Lab: Cell encapsulation, gel contraction, cell culture, microfabrication, gene delivery, microfluidics, BioMEMS

Language: Spanish (Native Bilingual), French (Limited Proficiency)

Clubs: Biomedical Engineering Society, Society for Professional Hispanic Engineers, Organización Latino Estudiantil

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