CURRICULUM VITAE

Dr Saba (Toxicology)

Post doctorate

Phone: +919*********

E-mail: ac1jun@r.postjobfree.com

OBJECTIVE

An enthusiastic, adaptive and fast-learning person with a broad and acute interest in the discovery of new innovative drugs. I believe to work with dedication and honesty in the field of medical science so that my skills can bestow better outcomes

Academic qualification

2016-2017 Post doctorates from University of Strasbourg, France

Advisor: Dr. Alberto Bianco

Research Topic: Intravenous injection of few layer graphene at 100 µg/ml into mice to evaluate different factors including the survival of the animals, organ accumulation and histology, blood parameters, and immune cell viability and activation.

2010-2015 PhD (Toxicology) from Department of Medical Elementology and Toxicology, Jamia Hamdard (Hamdard University), New Delhi, India.

Advisor: Dr. Sheikh Raisuddin (Advisor Research and Head of the Department).

Thesis Topic “Protective effects of walnut (Juglans regia) extract on combined effects of cyclophosphamide and bleomycin in rat lungs.

Course work Passed with 70%

2006-2008 M.Sc. (Toxicology) from Jamia Hamdard (Hamdard University), New Delhi, India with First division

2003- 2006 B.Sc. (Subjects: General English, Zoology, Biochemistry, Chemistry) from Womens College University of Kashmir, J&K, India, with Second Division

Professional experience

2009-2013 Meritorious research fellow, UGC-SAP-BRS, Govt. of India (1st two years as junior research fellow and current as senior research fellow)

2013-2015 CSIR-SRF fellow.

Teaching activities

During the course of Ph.D. remained involved in instructing M.Sc. (Toxicology) Students for their Lab. Course (2010-2013).

Present area of interest

My research work focuses mainly on the Toxicological events occurring in the lungs and its amelioration by plant based products. Other than lung toxicology part, I have already worked on evaluation of preventive potential of naturally occurring agents against hepatotoxicity.

Awards and achievements

2014 Awarded CSIR-SRF fellowship

2013 Received International travel grant by department of biotechnology (DBT) for presenting poster at the 49th congress of European Society of Toxicology, Interlaken Switzerland.

2013 Received a travel grant by Eurotox-2013 for presenting poster at the 49th congress of European Society of Toxicology, Interlaken Switzerland held in Switzerland.

2013 Received certificate of recognition of my research work and publication in high impact factor journal.

2012 Awarded a travel grant in international conference of society of free radical research (SFRR) held in Kolkata.

2011 Awarded meritorious research fellowship under UGC-SAP BRS-I Scheme of UGC (University Grants Commission), Govt. of India.

Training and symposium attended

Attended symposium on contemporary regulatory scenario in pharmacovigilance from 5th- 6th March, 2014

3 months industrial training in Drug Safety Evaluation R&D in Ranbaxy Research Laboratories, Gurgaon (India) from 10th June- 12th July 2009. Title of the project: Effect of carbon tetrachloride on hepatic biomarkers in Wistar rat.

2months training in forensic science lab, Srinagar, Kashmir. Had been involved in various poisoning cases, trap cases and explosive cases from 20th June- 20th July, 2010.

Training in recognition of BD FACSOrient (BD FACS Calibur) in flow cytometry basics from 16th -18th April, 2013 at BD-JH FACS Academy, New Delhi.

Participated in workshop on patenting process in the Department of Toxicology from 22th -23th Nov, 2011

Techniques and experimental expertise

Lung toxicity evaluation Bronchoalveolar lavage fluid (BALF) collection, cell isolation from BALF, estimation of cytotoxicity markers (lactate dehydrogenase, alkaline phosphatase) in BALF, phospholipid estimation in BALF, GSH in BALF and histopathological and immunohistochemical evaluation of lungs.

Molecular Biology DNA and RNA isolation, ELISA, Electrophoresis, Western blotting, PCR, Real Time PCR, FACS analysis, cell culture.

Staining Techniques Histology (H & E and Masson Trichrome Stain, Immunohistochemistry, Immunofloresence.

Oxidative stress studies Reduced glutathione (GSH), Lipid peroxidation, Xanthine oxidase activity, spectrophotometrically enzymes kinetic assay (Superoxide dismutase, Glutathione peroxidase, Glutathione-S transferase, Glutathione reductase, Catalase), Hydrogen peroxide level.

Inflammatory studies Expression of NFκB, TNFα, PGE-2 and IFNγ by ELISA, Neutrophil infiltration study by histopathology, Caspase-3, COX-2 and i-NOS by immunohistochemistry.

Biochemical studies Protein, Glucose, Cholesterol, Bilirubin, Creatinine, Activity of AST, ALT, Alkaline Phosphatase, AChE, MAO and Na+ K+ ATPase.

Animal handling Handling and conducting pharmacological animal experiments for various disease models handling pharmacology instruments. Efficient in animal handling especially mice (Swiss albino mice), rats (Wistar rats). Efficient handling in fish especially Channa punctata Bloch.

Routes of Administration Intratracheal (i.t.), gavages, intraperitoneal (i.p.), subcutaneous (s.c.), intrarectal (i.r.), intramuscular (i.m.).

Pharmacology Lab Techniques Invivo surgical preparations: tracheal intubation, and arterial and venous cannulations.

Behavioral pharmacology Develop animal behavioral models to screen chemical compounds with therapeutic potentials.

Environmental pharmacology Endocrine disrupting chemicals and mitochondrial toxicity.

Measurement Dose response curves

Others Collection of Liver, lungs, kidney, gonads, brain from the animal models and in Fishes. Homogenization of tissue samples, collection of various cell fractions by centrifugation/ultracentrifugation, Mitochondrial Isolation, Histopathological and immunohistochemical analysis of liver samples.

Research experience

4 years of experience in research work in Jamia Hamdard University

Research Paper (online/Accepted/Under Review)

The accepted paper of Saba can be cited as

Saba, Khan S, Parvez S, Chaudhari B, Ahmad F, Anjum S, Raisuddin S (2013). Ellagic acid attenuates bleomycin and cyclophosphamide induced acute pulmonary toxicity in Wistar rats. Food Chem Toxicol 58:210-9 (Online).

Beigh S, Rashid H, Sharma S, Parvez S, Raisuddin S (2017). Bleomycin-induced pulmonary toxicopathological changes in rats and its prevention by walnut extract. Biomed. Pharmacother. (in press). Date of acceptance - July 24, 2017.

Khan S, Beigh S, Chaudhari BP, Sharma S, Aliul Hasan Abdi S, Ahmad S, Ahmad F, Parvez S, Raisuddin S.

(2016). Mitochondrial dysfunction induced by bisphenol A is a factor of its hepatotoxicity in rats. Environ Toxicol 31:1922-1934 (Online)

Saba Beigh, Sheikh Raisuddin Ellagic acid ameliorates bleomycin induced lung toxicity in Wistar rats. J drug metab toxicol. doi: 10.4172/2157-7609.S1.002 (online).

Saba Beigh, Sameya Anjum, Firoz Ahmed, Sheikh Raisuddin (2017). Ellagic acid attenuates bleomycin and cyclophosphamide induced combinational toxicity by reducing INOS, COX -2, NF-κB And Caspase-3 Expression in rat lungs. Respiratory research (Under Review).

Hina Rashid, Firoz Ahmad, Saba Beigh, Shikha Sharma, Shahzad Ahmad, Syad Aliul Hasan Abdi and Sheikh Raisuddin (2016). Chronic low level exposure to bisphenol A induces changes in hormonal level in anaemic rats but less remarkable histological changes in gonads.Reproductive toxicology (Under Review).

Sameya Anjum, Saba Beigh, Firoz Ahmad and Sheikh Raisuddin (2016). Bisphenol induces apoptosis via the intrinsic mitochondria pathway in testes of Swiss albino mice. Andrology. (Under review).

Sameya Anjum, Banjit Bastia, Saba Beigh, Arun Kumar Jain, and Sheikh Raisuddin (2017). Bisphenol A induces disrupted spermatogenesis via oxidative stress and mitochondrial toxicity in the testis of adult male mouse. Toxicology (Under review).

Conferences attended and abstracts published

Saba, Firoz ahmad, Sameya anjum, Suhel Parvez, Sheikh Raisuddin. “Ellagic acid protects against cyclophosphamide induced acute lung injury in Wistar rats by inhibiting oxidative stress, NF-κB activation and inflammatory cell production”. Abstract published in eurotox-2013 held in Switzerland in Sep 1-4-2103.

Saba and Sheikh Raisuddin. Ellagic acid Ameliorates Bleomycin and Cyclophosphamide Induced Pulmonary Toxicity in Male Rats. Abstract published in abstract book of “The XIII international congress of toxicology” held in Coex, Seoul, Korea in June 30 - July 4, 2013.

Saba, Somaira Khan, Suhel Parvez, Sheikh Raisuddin. Juglans regia L. (Walnut) extract ameliorates cyclophosphamide induced acute lung injury in male rats. Abstract published in abstract book of 8th Congress of Toxicology in Developing Countries (CTDC8) held in Bangkok, Thailand in Sep 10-14-2012.

Saba, Firoz Ahmad, Suhel Parvez, Sheikh Raisuddin. Protective effect of Walnut (Juglans regia L.) extract against cyclophosphamide induced biochemical and histological alterations in rat lungs. Abstract published in abstract book of 12th Annual conference of Society for Free radical Research in India (SFFR India) and international conference on “Advances in Free Radicals, Redox Signaling and Translational Antioxidant Research & XII Annual meeting of the Society for Free Radical Research, India on 30th Jan- 1st Feb, 2013.

Saba, Somaira khan, Firoz Ahmad, Suhel Parvez, Sheikh Raisuddin. Pulmonary toxicity of combination therapy of bleomycin and cyclophosphamide in wistar rat lungs. Abstract published in abstract book of 11th Annual Conference of Society for Free radical Research in India (SFRR India) and International conference on “Emerging Trends in Free Radicals, Antioxidants and nutraceuticals on Health, Disease and Radiation Biology” held in Kolkata (India) on 1st jan- 3 feb, 2012.

Somaira khan, Saba, Firoz Ahmad, Suhel Parvez, Sheikh Raisuddin. Protective effect of vitamin E against Bisphenol A induced biochemical alterations in rat lung mitochondria. Abstract published in abstract book of 11th Annual Conference of Society for Free radical Research in India (SFRR India) and International conference on “Emerging Trends in Free Radicals, Antioxidants and nutraceuticals on Health, Disease and Radiation Biology” held in Kolkata (India) on 1st Jan- 3 Feb, 2012.

Firoz Ahmed, Hina Rashid, Saba, Somaira Khan, Manpreet Kaur, Hasib ur Rehman, S Raisuddin. Protective effect of unani formulation (Jawarish Amla sada) on cyclophosphamide induced toxicity in mice. Abstract published in abstract book of 30th annual Conference of Society of Toxicology held in jamia Hamdard, New Delhi in 2010.

Hina Rashid, Firoz Ahmed, Somaira Khan, Saba, Sheikh Raisuddin. Iron deficiency augments the effect of Bisphenol A on hormonal level in male rats. Abstract published in abstract book of 30th annual Conference of Society of Toxicology held in jamia Hamdard, New Delhi in 2010.

Membership

Member of “Society for Free Radical Research International (SFRR)”

Member of “Society of Toxicology, (STOX), India”

Computer skills

Preparing Power point presentations, Word Documents, Editing, Formatting etc.

Net browsing, scientific literature search, other essential parts of computer usage (installation of software, hardware problems etc.)

Linguistic ability

English: Speaking, reading and writing.

Urdu: Speaking, reading and writing.

Hindi: Speaking.

Arabic: Reading and learning to speak.

Other activities

Working as a Kashmiri Voice over Artist

Thesis summary and research areas worked on

Lungs are the most vulnerable to changes when exposed to toxicants and drugs because of having a large surface area and thin epithelium which makes them more susceptible towards xenobiotics and causes oxidative burden on the lungs. Studies revealed that oxidative stress in lungs generates reactive oxygen species (ROS) which initiates and impregnates various disorders such as chronic bonchites, fibrosis, emphysema, acute lung toxicity and cancers. Remodeling in lung epithelial cells may cause architectural and functional disruptions in lungs. ROS are also constantly formed in the human body as byproducts of normal metabolism and are removed by antioxidant defense mechanism. The lungs have efficient antioxidative defense mechanism and the primary defense mechanisms against oxidant stress are antioxidant enzymes (AOEs). Natural products with antioxidant properties show protective effect against chemically-induced lung toxicity. In recent years, there has been a growing research activity in understanding the importance of various phytochemicals, flavonoids and phenyl propanoids for their possible mechanisms of action in inhibiting oxidative stress. CP and other anticancer drugs have been used in combination with various detoxifying and protective agents with the purpose of reducing or eliminating their toxic effects. Natural compounds such as carboxymethylpachymaran, epigallocatechin-3-gallate, melatonin and ellagic acid (EA) have shown efficacy in reducing toxicities caused by BLM and CP. we studied protective effect of EA in rats exposed to BLM and CP with special reference to its protective effect against drug-induced lung toxicity. I have also studied the protective effect of Walnut extract and its major constituent Ellagic acid (through HPTLC analysis) against the toxic effect induced by BLM and CP.

References

Prof. Sheikh Raisuddin, Research Advisor and Head

Faculty of Science

Jamia Hamdard

New Delhi-110062

Email: ac1jun@r.postjobfree.com

Ph nb:098********

Dr. S. Chattopadhyay.F.N.A. Sc.

Head Bio organic Division

Baba atomic research Centre, Mumbai 400085

Email: ac1jun@r.postjobfree.com

Ph nb: 099********

Dr. Basharat Andrabi

Manager, Pharmacovigilance

Kinapse Ltd, Gurgaon 122002

Email: ac1jun@r.postjobfree.com

Ph nb: 077********

Date of birth 16th Aug 1983.

Father’s Name Gh. Ahmed Beigh.

Address Chinkral Mohalla, Habba Kadal, Srinagar.

All the details mentioned above are true to the best of my knowledge and nothing has been concealed.

Date: 24-7-2017

(SABA)

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