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Assistant Research

Location:
Santa Clara, CA
Posted:
July 20, 2017

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Resume:

EDUCATION

Bachelor of Science, Microbiology September 2013- June 2017 University of California, Riverside

EXPERTISE, SKILLS & KNOWLEDGE

Immunology, Parasitology, Genomics and Bioinformatics and life-cycles of various parasites.

Function of the Immune System responses to pathogens.

Pathology and epidemiology of viruses.

Use of Online Databanks and Tools such as the RCSB Protein Data Bank and BLASTs provided by NCBI.

Learned to conduct DNA/RNA extraction and Polymerase chain reaction experiments and qPCR analysis.

Experienced in Flow Cytometry procedures for detection of biomarkers, cell sorting and counting with hands on expertise in fluorescence activated sorting using green fluorescent protein markers.

Lab experience in High Performance Liquid Chromatography (HPLC) and Spectroscopic analysis.

Proficiency and practical expertise in executing cell based and immunoassays (ELISA).

Practical knowledgeable in Reagent preparation, storage and handling.

Experience in setting up and running Gel Electrophoresis.

Knowledgeable in mass spectrometry and Protein Analysis Assays.

Skilled in Streak and Spread Plating and procedures for Gram and Endospore Staining.

Knowledgeable in care and use of laboratory equipment for cell culture, microscopy and other assays.

Skilled in purification of chemical compounds using Separatory funnels, Recrystallization and Distillation.

Theoretical knowledge of many cellular, analytical and immunoassay techniques including Western Blot.

Theoretical knowledge of Sanger and Next -generation sequencing methods.

PROFESSIONAL AND RESEARCH EXPERIENCE

Dr. Sarjeet Gill’s lab, UC Riverside, Riverside CA November 2014-June 2016 Research Assistant

Led breeding of transgenic mosquitoes: maintained the mosquito colony, prepared reagents and assisted researchers in performing assays. This involved refilling sugar water vials, hatching eggs and using a fluorescent microscope to screen the larvae for a GFP marker linked to the modified gene.

Handled and bred mosquitoes with required phenotype and maintained sufficient supply of modified mosquitoes for researchers.

Maintained and cleaned lab equipment, such the decalcifying mosquito colony's humidifier every month.

Gained proficiency in dilutions, breeding and using fluorescent microscopes.

Molecular Medicine Research Institute (MMRI), Sunnyvale CA June 2014

Summer Intern

Assisted various scientists with different research projects and conducted DNA extraction, passaging adherent cells, counting cells, loading cells into wells and completing cell cultures for a variety of cell types grown for different research teams.

Gained experience with the proper use of the following equipment and techniques;

Incubators, Cell culture vessels (flasks, Petri dishes, roller bottles, multi-well plates), Aspiration using vacuum pump, Confocal microscope, Hemocytometer, Multichannel pipettes and Fume Hoods.

Learned lab procedures for Thin Layer Chromatography, Vacuum Filtration, Mass Spectrometry and Autoclaving.

Experienced in proper handling and cleanup of hazardous chemicals

INTERESTS

Strong desire to pursue graduate studies in the field of Virology.

Amnesty International - Participated, conducted research and acted to prevent and stop abuses of human rights.

Water Polo - Member of Lynbrook’s Varsity water polo team for 3 years.

SCUBA certified (PADI) - Previously certified as Life Guard and provided lifeguard services at community pools.



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