State University Assistant

Dear sir,

I am writing to apply for the position advertised on web. The opportunity presented in this listing is very interesting, and I believe that my strong education and technical experience will make me a very competitive candidate for this position. The key strengths that I possess for success in this position include:

With the BS and MS degrees in microbiology and Ph.D in biochemistry, I have a full knowledge on molecular biology, microbiology, and pathobiology. I have done research works in many different fields of biology and obtained a lot of research experiences, such as microorganism isolation and characterization, gene clone, real time PCR, protein analysis, DNA-protein binding, microarray, tissue culture, microbial infection, immunofluorescence staining, cell and animal imaging in vivo, etc. I also have experience in learning and excelling at new technologies as needed.

Please see my resume for additional information on my experience.

I can be reached anytime via email at **********@*****.*** or my home phone, 225-***-****

Thank you for your time and consideration. I look forward to hear from you about this employment opportunity.

Sincerely,

Yanlin Shi

CURRICULUM VITAE

Yanlin Shi

1235 Jim Taylor Dr

Baton Rouge, LA 70820

Phone: 225-***-****

Email: **********@*****.***

Education

Ph. D., Biochemistry

08/1999-08/2005, Louisiana State University, Baton Rouge, LA.

MS., Microbiology

08/1984-09/1987, Chinese Academy of Sciences, Beijing, China.

BS., Microbiology

02/1978-01/1982, Northwest University, Xian, China.

Positions and Employment

05/2011-07/2015 Research assistant professor

Louisiana State University, Department of Pathobiological Science, Baton Rouge, LA70803.

08/2010-04/2011 Sr. research assistant

University of Texas, Health Science Center, Medical School at Houston, Department of Pediatric Surgery, Houston, TX77030.

04/2008-08/2010 Postdoctoral research associate

Texas A&M University, Health Science Center, Department of Microbial and Molecular Pathogenesis, College Station, TX77843

08/2005-03/2008 Postdoctoral research associate

Louisiana State University, Department of Pathobiological Science, Baton Rouge, LA70803

08/1999-08/2005 Teaching assistant

Louisiana State University, Department of Biological Sciences, Baton Rouge, LA77802.

Research experiences

With the Ph.D in biochemistry and many years’ research experiences in different medical labs, I have a full knowledge on molecular biology and pathobiology. I have done research works in many different fields of biology and obtained a lot of research experiences, such as extract DNA, RNA, protein from different animal, plant, or bacteria, DNA cloning and gene transformation, DNA cycle sequencing, real time PCR, Genotyping, genome analysis, protein analysis, DNA-protein binding, microarray, tissue culture, microbial infection, immunofluorescence staining, cell and animal imaging in vivo, etc. I am familiar with different sequencing software, capillary electrophoresis. I took some courses on statistical during PhD study and used statistical analysis during research.

In Louisiana State University, School of Veterinary Medicine (08/2005-03/2008, as a postdoctoral research associate, 05/2011- present, as a research assistant professor), my research focus on Lyme disease, studying how gene change in bacteria can affect its infectivity in animal, including Borrelia strain geome analysis, impotant gene' knock out and completmentation, gene expression analysis. In Texas A&M University, Medical School (04/2008-08/2010, as a postdoctoral research associate), I study Tuberculosis, using in vivo imaging system to detect TB in animal, incuding insert gene probe into TB genome, detect signal in vivo. In The University of Texas, Medical School at Houston (08/2010-04/2011, as a Sr. research assistant), Health Science Center at Houston, Medical School, I did research on gastroschisis, using different methods to detect animal tissue histological change during gastoschisis, such as using different protein antibodies to label and observe change of tissue on microscope.

Teaching experiences

In Louisiana State University, Department of Biology (08/1999-08/2005, as a teaching assistant), I taught General Microbiolgy (BIOL 2051) lab class for 5 years and Basic Biochemistry (BIOL 4087) lab class for one year.

Selected publications

1.Shi Y., Dadhwal P., Li X., Liang F.T. (2014). BosR functions as a repressor of the ospAB operon in Borrelia burgdorferi. PLoS One. 2014; 9(10):e109307.

2.Xu Q., Shi Y., Dadhwal P., Liang F.T. (2012). RpoS regulates essential virulence factors remaining to be identified in Borrelia burgdorferi. PLoS One. 2012; 7(12): e53212.

3.Kong Y., Shi Y., Chang M., Akin A.R., Francis K.P., Zhang N., Troy T.L., Yao H., Rao J., Cirillo S.L.G., Cirillo J.D. (2011). Whole-body imaging of infection using bioluminescence. Current Protocols In Microbiology Issue: SUPPL.21 (2011-01-01) ISSN: 1934-8525.

4.Shi Y, S. L. G. Cirillo, Y. Kong, M. Chang, K. P. Francis, and J. D Cirillo. Detection of mycobacterium with b-galactosidase in vitro and in vivo (2010). Poster on 110th General Meeting of the American Society for Microbiology. San Diego, CA.

5.Cirillo S.L.G., S. Subbian, Y. Kong, M.Chang, H. Nawar, Y, Shi, and J. D. Cirillo. (2008). Molecular imaging strategies for tuberculosis: Visualizing the white plague. Poster on 2008 world molecular imaging congress. Nice, France.

6.Shi Y, Xu Q, Seemanaplli SV, McShan K, Liang FT. (2008). Common and unique contributions of decorin-binding proteins A and B to the overall virulence of Borrelia burgdorferi. PLoS One. 3(10):e3340.

7.Shi Y, Xu Q, McShan K, Liang FT. (2008). Both decorin-binding proteins A and B are critical for the overall virulence of Borrelia burgdorferi. Infect Immun. 76(3):1239-46.

8.Shi Y, Xu Q, Seemanapalli SV, McShan K, Liang FT. (2006). The dbpBA locus of Borrelia burgdorferi is not essential for infection of mice. Infect Immun. 74(11):6509-12.

9.Shi Y, Zhang Y, Shih DS. (2006). Cloning and expression analysis of two beta-1,3-glucanase genes from strawberry. J Plant Physiol. 163(9):956-67.

10.Shi Y, Shih DS. (2006). Identification of a zinc transporter gene in strawberry. DNA Seq. 2006 Feb;17(1):15-23.

11.Liu Z, Shi Y, Zhang Y, Zhou Z, Lu Z, Li W, Huang Y, RodrÌguez C, Goodfellow M. (2005). Classification of Streptomyces griseus (Krainsky 1914) Waksman and Henrici 1948 and related species and the transfer of 'Microstreptospora cinerea' to the genus Streptomyces as Streptomyces yanii sp. nov. Int J Syst Evol Microbiol. 55(Pt 4):1605-10.

12.Zhang S, Rush M, Shih D, Shi Y, Groth D. (2005). Quantification of transgene expression in transgenic rice plants with different levels of rice sheath blight resistance. PHYTOPATHOLOGY 95 (6): S117-S117.

13.Khan AA, Shi Y, Shih DS. (2003). Cloning and partial characterization of a beta-1,3-glucanase gene from strawberry. DNA Seq. 2003 Dec;14(6):406-12.

14.Lu Z, Wang L, Zhang Y, Shi Y, Liu Z, Quintana ET, Goodfellow M. (2003). Actinomadura catellatispora sp. nov. and Actinomadura glauciflava sp. nov., from a sewage ditch and soil in southern China. Int J Syst Evol Microbiol. 53(Pt 1):137-42.

15.Wang LM, Zhang YM, Lu ZT, Shi YL, Liu ZH, Maldonado L, Goodfellow M. (2001). Nocardia beijingensis sp nov., a novel isolate from soil. Int J Syst Evol Microbiol. 51: 1783-1788.

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