John R. Haserick, Ph.D.

E-mail: ac19p0@r.postjobfree.com

linkedin.com/in/john-haserick

SUMMARY

Over 15 years of laboratory experience in both academic research and industrial R&D, I excel in collaborative environments, developing novel methods and techniques to solve challenging technical problems. I have five years of hands-on experience using high-resolution mass spectrometers and developing LC-MS/MS methods to map post-translational modifications on proteins including: phosphorylation, O- and N-glycosylation, and lipidation. I have demonstrated success identifying unknown structures using state-of-art instruments and techniques (eg. FT-ICR EED MS/MS) and using established methods (GC-MS; QIT MSn). I am a self-motivated researcher with a high degree of independence, with an established track record of productivity and the ability to work with collaborators from diverse backgrounds.

EXPERIENCE

Boston University School of Medicine, Analytical Instrumentation Core, Boston, MA 2017–present

Technical Consultant

Providing consultation on instrumentation setup and utilization for peptide, glycan, VOC, and lipid analyses

Establishing analytical workflows and semi-automated reporting systems for VOC and FAME analyses

Training the core personnel on mass spectrometry, LC, and GC instrumentation

Boston University School of Medicine, Center for Biological Mass Spectrometry, Boston, MA 2011–2017

Ph.D. Candidate

Proteomic and glycomic analysis of human pathogens, focused on novel glycoconjugates

Effectively collaborated in a parasitology/glycobiology and an analytical mass spectrometry lab, advancing research in proteomics, glycoproteomics, and glycomics.

Completed all aspects of experimental design, sample preparation, LC-MS/MS method development, data analysis, and interpretation of results with complex bio-samples

Developed methods for discovery, characterization, and mapping of protein modifications including: phosphorylation, O- and N-glycosylation, and lipidation

A specialist in the analysis of N- and O-glycosylated proteins and in structural characterization of oligosaccharides

Highly skilled in interpretation of MS/MS spectra from CID, HCD, and ExD fragmentation techniques

Extensive hands-on experience setting up, running, optimizing, and maintaining numerous mass spectrometers and UPLC/HPLC instruments

Proteomics and data analysis expert with most commercial software suites (see skills and techniques)

Achievements:

Produced the first detailed description of the N-glycosylated and O-glycosylated proteins from the human pathogen Cryptosporidium parvum

Provided structural characterization of the N-glycans from C. parvum using FT-ICR EED MS/MS

Mapped the post transitional modifications of vaccine candidate and immunodominant proteins from C. parvum, paving the way for future vaccine development and improved serodiagnostic tools

Method development of CE-MS for monosaccharide analysis

Shared the discovery of O-fucosylated proteins from the pathogen T. gondii

Monosaccharide composition analysis of the N-linked octasaccharide from M. mazei

BioPhysics Assay Laboratory, Worcester, MA 2008–2011

Senior Scientist, Research and Development

Manufacturer of novel nanoparticles used by researchers

Worked with a multidisciplinary group of scientists developing nanoparticles used for labeling and tracking cells, spanning the disciplines of chemistry and cell biology, bringing products through development to the market.

Envisioned new products and their applications, gauging market demand and customer needs

Designed and synthesized novel nanoparticles, testing on multiple cell lines

Performed bioconjugations with proteins and carbohydrates, adding additional functional groups, pharmaceuticals, flurophores, and MRI contrast agents

Maintained meticulous records, wrote reports, and authored application notes for new products

Interacted with customers, helping them optimize their methods

Achievements:

Designed a reliable and reproducible method for manufacturing iron oxide nanoparticles, solving the problems of reproducibility and variability in quality

Developed multiple products currently on the market, including products used for cell labeling and in vivo tracking, therapeutics/diagnostics, and separation of dead cells

Products brought to market include: Molday ION C2Amine™, Molday ION Aromatic Amine™, Molday ION Carboxyl Terminated™, Molday ION™ (EverGreen, Coumarin, Rose Bengal, and Dye Free) and the Dead-Cell Removal Kit - Viahance™

University of Connecticut, Storrs, CT 2002–2008

M.S. Student, Researcher 2005–2008

B.S. Student, Research Assistant 2002–2005

Laboratory focused on the underlying mechanisms driving cell movement

Focused on studying the mechanisms of cell movement, with an emphasis on the use of live cell imaging microscopic techniques

Achievements:

Successfully designed, built, and tested an apparatus to manipulate cell movement on a microscope stage, providing the capability of studying the underlying mechanisms of cell motility with live cells

Transiently transfected primary fish epithelial cells with GFP-actin allowing for the visualization of actin dynamics during cell movement

SKILLS AND TECHNIQUES

Mass Spectrometry:

Expertise utilizing LC-MS/MS, GC-MS, CE-MS, ESI/nESI-MS/MS, and MALDI-TOF-MS to gain a deep understanding of complex biological samples for mapping post-translational modifications on proteins and identification of unknown structures

Instrumentation:

Extensive hands-on experience running: Thermo Fisher Scientific: (LTQ-XL-ETD Orbitrap, QE+, and QE-HF); Bruker: (AmaZon Speed QIT with ETD, SolariX 12-T FT-ICR MS, Scion SQ GC-MS, UltrafleXtreme MALDI-TOF/TOF MS) mass spectrometers

User familiar with Agilent: (6550 QTOF, 6520 QTOF, and 6890N GC-MS) instruments

Data Interpretation and Software:

Proven ability of manual interpretation of glycopeptide, peptide, and glycan MS/MS spectra using: Thermo Xcalibur Qual Browser, Bruker DataAnalysis/FlexAnalysis, and Agilent Mass Hunter

Proficiency using Thermo Proteome Discoverer, PEAKS Studio, Mascot, and Scaffold for proteomics

Liquid Chromatography:

Maintained functionality, operated, and managed the use of a Waters nanoACQUITY UPLC and an Agilent 1260 Infinity for over 3 years

User familiar with Agilent: 1200 Series HPLC; Beckman Coulter: Gold HPLC Systems

Sample Preparation and Purification:

Performed: affinity, size exclusion, and ion exchange chromatography, solid phase extractions, SDS-PAGE separations, tangential flow and stirred cell ultrafiltration/concentrations, and dialysis

Optimized extraction methods for proteins and carbohydrates from limited amounts of sample

Enzymatic digestions of proteins for LC-MS/MS analyses

Chemistry:

Carbohydrate derivatization (permethylation, per-acetylation) for LC-MS/MS, GC-MS, and MS/MS experiments for mono- and oligosaccharide identification and structural analyses

Bioconjugations: modification of proteins/antibodies with fluorophores, Gd-DTPA, and conjugation to nanoparticles; modification of polysaccharides and oligosaccharides with functional groups for attachment of ligands, and reducing end labeling.

Synthesis of cross-linked nanoparticles, surface modifications to add functional groups for additional conjugations

Cell Biology and Microscopy:

Sterile technique, cryopreservation, viability assays, proliferation assays, growth and maintenance of cell lines and primary isolates, inhibitor studies, cell motility assays, cell fixing and staining (chemical, antibody, and lectin), and transient transfections

Microscopic right field, phase contrast, DIC, fluorescent, and confocal)

Molecular Biology:

PCR amplification, cloning, and expression of recombinant proteins in bacteria.

Computer:

MS Office; Graphics Programs: Adobe Illustrator, Adobe Photoshop, Inkscape, GIMP; Operating systems: Microsoft Windows, OS/X, and Linux/Unix

General Lab Techniques:

SDS-PAGE, TLC, ELISA, UV-Vis-NIR spectrophotometry, fluorescent spectroscopy, dynamic light scattering particle sizing

EDUCATION

Ph.D., Biochemistry, Boston University School of Medicine, Boston, MA 2017

The Unique Glycoproteins of Cryptosporidium parvum and Toxoplasma gondii

M.S., Cell Biology, University of Connecticut, Storrs, CT 2010

Design, Manufacturing, and Implementation of a Galvanotactic Chamber as a Tool for Cell Biology

B.S., Molecular and Cell Biology, University of Connecticut, Storrs, CT 2005

PUBLICATIONS

Haserick, J.R.; Klein, J.; Costello, C.E.; Samuelson, J., (2017). Cryptosporidium parvum Vaccine Candidates are Heavily Modified with O-linked-N-acetylgalactosamine or Contain N-terminal N-Myristate and S-Palmitate. PLoS ONE, 12(8): e0182395.

Khatri, K.; Klein, J.; Haserick, J.R.; Leon, D.; Costello, C. E.; McComb, M. E.; Zaia, J. (2017). Microfluidics Capillary Electrophoresis - Mass Spectrometry for Analysis of Monosaccharides, Oligosaccharides and Glycopeptides. Analytical Chemistry, 89 (12), 6645-6655

Haserick, J.R., Leon, D.R., Samuelson, J. and Costello, C.E., (2017). Asparagine-linked Glycans of Cryptosporidium parvum Contain a Single Long Arm, Are Barely Processed in the ER or Golgi, and Show a Strong Bias for Sites with Threonine. Molecular & Cellular Proteomics, 16, S42-S53.

Bandini, G., Haserick, J.R., Motari, E., Ouologuem, D.T., Lourido, S., Roos, D.S., Costello, C.E., Robbins, P.W. and Samuelson, J., (2016). O-Fucosylated Glycoproteins Form Assemblies in Close Proximity to the Nuclear Pore Complexes of Toxoplasma gondii. Proceedings of the National Academy of Sciences U S A, 113, 115**-*****.

Jurado, C., Haserick, J.R. and Lee, J., (2005). Slipping or Gripping? Fluorescent Speckle Microscopy in Fish Keratocytes Reveals Two Different Mechanisms for Generating a Retrograde Flow of Actin. Molecular Biology of the Cell, 16, 507-518.

PRESENTATIONS

Haserick, J. R.; Leon, D. R.; Pu, Y.; Samuelson, J. C.; Costello, C. E., Mass Spectrometric Analysis of the Human Pathogen Cryptosporidium parvum. 64th Annual Conference of the American Society for Mass Spectrometry, San Antonio, TX, June 5-9, 2016.

Haserick, J. R.; Costello, C. E.; Samuelson, J. C., Mass Spectrometry of Glycopeptides from Cryptosporidium parvum. Molecular Parasitology Meeting XXVI, Woods Hole, MA, September 20-24, 2015.

Haserick, J.R.; Samuelson, J.; Costello, C. E., De novo Sequencing of the Glycoproteins from Cryptosporidium parvum. Gordon Research Conference, Carbohydrates: Frontiers in Basic and Translational Glycosciences, June 14-19, 2015, Dover, VT.

Haserick, J.R.; Costello, C. E.; Samuelson, J., De novo Sequencing of the Glycoproteins of Cryptosporidium parvum. Science Day, Henry M. Goldman School of Dental Medicine, March 19, 2015, Boston, MA

Haserick, J.R.; Costello, C. E.; Samuelson, J., Carbohydrate Structures in the Oocyst Walls of C. parvum. Molecular Parasitology Meeting XXV, Woods Hole, MA, September 14-18, 2014.

Haserick, J.R.; Costello, C. E.; Samuelson, J., Glycomics of the parasite Cryptosporidium parvum. The New England Association of Parasitologists, paraINsites 2014: From Protozoa to Worms, Boston, MA, April 12, 2014.

Haserick, J.R.; Motari, E.; Carpentieri, A.; Bushkin, G. G.; Duchene, M.; Costello, C. E.; Robbins, P. W.; Samuelson, J., Protective Monoclonal Antibodies to Entamoeba Target Dextran-like Phosphoglycans Present on a Diverse Set of Amebic Glycoproteins. Molecular Parasitology Meeting XXIV, Woods Hole, MA, September 8 - 12, 2013.

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