research scientist
Resume:
Jeff Thomas
Sewell, NJ *****
ac14cf@r.postjobfree.com
EDUCATION:
Drexel University College of Medicine (Philadelphia, PA), received Ph.D. in biology in Molecular Pathobiology program in May 2013
Thesis project: My research project entailed understanding the pathways involved in translational repression that occurs during hypoxia in cancer cells.
Advisor: Gregg Johannes Ph.D
Drexel University (Philadelphia, PA), Received M.S. degree in biology in 2003.
Thesis project: Characterizing regulation of the orfG promoter during stationary phase in cyanobacteria.
Advisor: Shivanthi Anandan Ph.D
Richard Stockton College (Pomona, NJ), Received B.S. degree in biology in 1998.
RELEVENT EXPERIENCE:
Research Scientist, Drexel University College of Medicine/Context Therapeutics (Philadelphia, PA) 4/15- Present
My research focuses on targeting the sigma 1 protein in prostate cancer through through using novel sigma 1 antagonists to inhibit the Androgen Receptor axis of prostate cancer progression.
Adjunct Professor of Biology, Rowan University (Glassboro, NJ) Fall 2015 Semester-Present
I have taught the laboratory sections for a General Biology class that is for non-biology majors along with both genetics, and cell biology classes for biomedical engineering majors.
Post-Doc Fellow, Children’s Hospital of Philadelphia (Philadelphia, PA) 8/13-4/15
My project is focused on understanding the role of the unfolded protein response pathway for the ability of plasma cells to cope with ER stress resulting from continuous antibody production. I am also interested in understanding the role the UPR has on the differences in survival of short-lived vs. long lived plasma cells.
Senior Research Assistant, Drexel University College of Medicine (Philadelphia, PA) 7/03 – 7/07
Identified mRNAs that are translated efficiently through microarray analysis during hypoxia.
Directly supervised two Ph.D students and trained them in various molecular biology techniques.
Responsible for ordering supplies, keeping inventory databases, keeping track of laboratory budget, maintaining logs for radiation usage in the lab.
Teaching Assistant, Drexel University (Philadelphia, PA) 1/01 – 6/03
Responsible for teaching recitations and lab courses to undergraduate students in a variety of courses.
These classes included: microbiology, genetics, developmental biology, biochemistry, and advanced cell biology.
Laboratory Technician, Coriell Institute for Medical Research (Camden, NJ) 11/98 – 1/01
Responsible for isolating lymphocytes from blood samples and maintaining lymphocyte cell cultures.
Laboratory Technician, Richard Stockton College (Pomona, NJ) summer of 1997 +1998
Responsible for isolating DNA from bacterial samples from various geothermal wells that were on campus.
PEER-REVIEWED PUBLICATIONS:
1. Thomas JD, Longen CG, Oyer HM, Chen N, Maher CM, Kania B, Salvino, JM, Anderson KN, Ostrander W, Knudsen KE, Kim FJ. Sigma 1 Targeting to Suppress Aberrant Androgen Receptor Signaling in Prostate Cancer. Cancer Research 2017 Feb. 15
2. Zhang L, Zhai J, Mojsilovic-Petrovic J, Jian X, Thomas JD, Homma K, Scmitz A, Famulok M, Ichijo H, Argon Y, Randazzo P, Kalb R. Inhibition of cytohesins protects against genetic models of motor neuron disease. The Journal of Neuroscience. 2015 Jun. 17
3. Peng M, Ostrovsky J, Koon Y, Tsukikawa M, Licata J, Polyak E, Thomas JD, Felix C, Xiao R, Gasser D, Argon, Y, Falk M. Inhibiting cytosolic translation and autophagy improves health in mitochondrial diseases. Hum Mol Genet. 2015 Jun 3
4. Popnikolov NK, Dalwadi BH, Thomas JD, Johannes GJ, Imagawa WT. Association of autotaxin and lysophosphatidic acid receptor 3 with aggressivnesss of human breast carcinoma. Tumour Biol. 2012 Aug 26.
5. Crowe E, Sell C, Thomas JD, Johannes GJ, Torres C. Activation of proteasome by insulin-like growth factor-1 may enhance clearance of oxidized proteins in the brain. Mech Ageing Dev. 2009 Nov-Dec; 130(11-12): 793-800.
6. Thomas JD, Dias LM, Johannes GJ. Translational repression during chronic hypoxia is dependent on glucose levels. RNA. 2008 Apr; 14(4): 771-81.
7. Thomas JD, Johannes GJ. Identification of mRNAs that continue to associate with polysomes during hypoxia. RNA. 2007 Jul; 13(7): 1116-31.
8. Dolloff NG, Shulby SS, Nelson AV, Stearns ME, Johannes GJ, Thomas JD, Meucci O, Fatatis A. Bone-metastatic potential of human prostate cancer cells correlates with Akt/PKB activation by alpha platelet-derived growth factor receptor. Oncogene. 2005 Oct 13; 24(45): 6848-54.
AWARDS:
1.April-2014 – April 2015. Awarded T32 Training Grant in the Immunology of Normal and Neoplastic Lymphocytes from the University of Pennsylvania.
2.September 2012. Received travel award from the alumni association of Drexel University College of Medicine to attend the translational control conference at Cold Spring Harbor Laboratory.
LABORATORY SKILLS:
Agarose gel electrphoresis, DNA isolation, cloning, PCR, RT-PCR, qRT-PCR, RNA isolation, northern and southern blotting, microarray analysis, polysome RNA purification, S35 metabolic labeling, luciferase assays, transfection of cells (transient, retrovirus and lentivirus), si- and shRNA mediated knockdown in cells, SDS-PAGE gel electrophoresis, native gel electrophoresis, western blotting, co-immunoprecipitation, silver staining, alamar blue assay, fluorescence microscopy, immobilized metal-affinity chromatography, flow cytometry, Elispot.
References:
1.Gregg Johannes Ph.D
Assistant Professor of Biology
Drexel University College of Medicine
ac14cf@r.postjobfree.com
2.Shivanthi Anandan Ph.D
Associate Professor of Biology
ac14cf@r.postjobfree.com
3.Maria Tahamont Ph.D
Professor of Biologial Sciences
Rowan University
856-***-**** ext 3579
ac14cf@r.postjobfree.com
.
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