Débora Torres Alves Figueirêdo

*** ********* **, ****, ***** Carolina, 27519

Home: 919-***-****- Cell: 919-***-**** - ac0wvy@r.postjobfree.com

PROFILE:

I have more than 10 years of experience in laboratory, since I started to work as a research assistant in the Catholic University where I used to attend my Biology degree. I am looking for an opportunity to work on an important institution that provides professional development, interesting experiences and personal growth.

Goal- oriented and highly motivated scientist

Extensive experience in molecular biology

Excellent communications skills and learning ability.

Excellent collaborative and interdisciplinary interactions

Excellent computer skills, proficient with MS Office and familiar with LIMS.

EDUCATION:

03/2013-03/2017: PhD Molecular Biology

University of Brasília – Brasília, DF, Brazil.

Research: Metabolic engineering in soybean (Glycine max) and genome editing in genes associated with fatty acids.

01/2010-02/2013: Master of Science: Molecular Pathology

University of Brasília – Brasília, DF, Brazil.

Research: Preliminary Functional Analyses of Dipeptidyl peptidase 8 and Otubain in Trypanosoma cruzi through knockout.

02/2005-12/2009: Bachelor of Science: Biology

Catholic University of Brasília – Brasília, DF, Brazil

Research: Purification of recombinant anti-HIV molecule in soybean seeds.

SKILLS:

Protein purification/ column chromatography

SDS-PAGE gel analysis

HPLC chromatography

Enzymatic assays

Heat shock/plasmid transformation

RNA/DNA extraction and purification

Gel electrophoresis/restriction analysis

PCR performance

Western/southern Blot

Experience with immunoassays (e.g. ELISA)

Experience with bioconjugation reactions

End Point qPCR;

Experience with FPLC and gel filtration

Knockout of genes through TALEN and Homologous Recombination.

Proficient with LIMS

LANGUAGE:

Excellent spoken and written English and Portuguese

PROFESSIONAL EXPERIENCE:

September 2016- Present: Lab Technician (Protein Analyst)

US Tech Solutions, Inc/ Bayer Crop Science

Perform protein extraction and quantitative ELISA-based protein expression levels in various plant tissues.

Execute technical experiments under direction of the Protein Analysis lead,

Followed standard operating procedures (SOPs).

Responsible for ELISA development and validation.

Receive, catalogue and inventory samples, including application of barcodes.

Complete high throughput sample grinding and DNA extraction from plant tissues using robotics.

Prepare and stock reagents and consumables for DNA extractions.

Trained to execute fluorescence based endpoint and Real-time PCR (qualitative and quantitative) in multiple agricultural plant species;

2013- 2016: PhD Candidate

University of Brasília

Design TALEN proteins to bind DNA sequences common to two enzymes involved in the fatty acid biosynthetic pathway, and construction of vectors for transformation.

Responsible for selection of transgenic plants

Use GC-MS to determinate oil content from soybean seeds.

Development of PCR, southern blot protocols to verify TALEN mutations.

Improvement of DNA extraction protocols specific for soybean.

Uses of ELISA immunoassay to verify presence of recombinant protein expressed in soybean.

Review, interpret, integrate, and present data on scientific meetings and conferences

2010-2013: Master Student

University of Brasília

Laboratório de Interação Parasito hospedeiro

Responsible for investigate protein function in Trypanosoma cruzi.

Development of knockout protocols using PCR products and vector construction.

Maintaining cell cultures

Development of Bioconjugation reactions

2008-2009: Undergraduate Researcher

Brazilian Agricultural Research Corporation EMBRAPA

Laboratory of Synthetic Biology, Cenargen

Performance of projects with purification and detection of recombinants molecules expressed in soybean using analytical HLPC, gel filtration chromatography, ELISA, Western Blot, Dot blot and PCR for selection of transgenic plants.

2005-2008: Undergraduate Researcher

Catholic University of Brasília

Centro de Análises Proteômicas e Bioquímicas.

Performance of projects with purification and characterization of different amylases inhibitors from plants, using protein extractions protocols, purification using Sepharose and Gel filtration columns and analytical HPLC, protein electrophoresis, western blot, EDMAN sequencing, enzyme assays using colorimetric methods and in vivo tests.

PUBLICATION:

ALVES, D. T. ; Vasconcelos, I. ; Oliveira, J. T. A. ; Farias, L. R. ; DIAS, S. C. ; Chiarello, M. D. ; Neto, S. M. ; Franco, O. L.. Identification of four novel members of Kunitz-like α-amylase inhibitors family from Delonix regia with activity toward Coleopteran insects. Pesticide Biochemistry and Physiology, v. 95, p. 166-172, 2009.

PRESENTATIONS:

ALVES, D. T. ; COELHO, M. C. F. ; Murad, A. M. ; VIANNA, G. R. ; COELHO, C. M. ; RECH, E. L. . ENGENHARIA METABÓLICA DE SOJA E EDIÇÃO DE GENOMA DE GENES ASSOCIADOS A ÁCIDOS GRAXOS. (Metabolic and genomic engineering of genes associated with polyunsaturated acids). In: XIX Encontro do Talento Estudantil, 2014, Brasilia. XIX Encontro do Talento Estudantil, 2014.

ALVES, D. T. ; MOTTA, F.N. ; FIGUEIREDO, G.S.F ; AZEVEDO, C. S. ; SANTANA, J. M. ; BASTOS, I. M. D. . Study of the role of Dipeptidyl-peptidase 8-like and Otubain genes in Trypanosoma cruzi infection. In: 10th International Congress on Cell Biology, 2012, Rio de Janeiro. 10th International Congress on Cell Biology, 2012.

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