Kathryn Reinicke

Oncology Research Scientist

***** ******* ****** *****

Brooklyn Park, MN 55445

Phone: 507-***-****

ac0vbi@r.postjobfree.com

EXECUTIVE PROFILE

Innovative and adept Research Scientist with a strong scientific record and proficiency in molecular biology and biochemical techniques. Successfully designs, develops, and validates research projects. Provides outstanding leadership and supervision to junior scientists and technical support staff. Possesses substantial expertise elucidating the mechanism of action of therapeutic compounds as well as understanding the genetic framework of cancer and genetic changes associated with tumor progression. Proficient in utilizing data from public resources such as PubMed as part of data analysis or method development. Practiced in writing scientific abstracts, peer-reviewed manuscripts, and grants. Effective presentation and verbal communication of scientific information.

PROFESSIONAL EXPERIENCE

Mayo Clinic, Rochester, MN 2007-2012

Research Fellow with Dr. Matthew M. Ames

Lead and assisted with qualitative data collection and analysis as well as manuscript development. Collected and interpreted experimental data, prepared study reports, and presented findings.

Designed and executed in vivo studies in models utilizing new technologies to evaluate tumor burden. Performed large-scale murine xenograft studies to determine the efficacy of endoxifen compared to other anticancer agents in vivo and analyzed the effect of endoxifen on the estrogen receptor’s ability to act as a transcription factor.

Elucidated the mechanism of action of endoxifen antitumor activity in breast cancer. Examined several cell signaling pathways for involvement in endoxifen-mediated growth inhibition in both Tamoxifen-sensitive and Tamoxifen-resistant cancer cell lines.

Developed and applied innovative approaches for oncology. Elucidated the mechanism of action of aminoflavone antitumor activity in breast cancer and utilized radioactive drugs to measure aminoflavone cellular uptake, and binding to cellular macromolecules and DNA.

Case Western Reserve University, Cleveland OH 2001-2006

Graduate Student with Dr. David A. Boothman

Directed and managed the elucidation of the novel pathway by which beta-lapachone induces apoptosis in cancer cells. Examined several cell death pathways (apoptosis, necrosis, programmed cell death) for involvement in beta-lapachone-induced cell death.

Utilized UV spectroscopy for analyses of prodrug conversion under acidic conditions. Determined that beta-lapachone treatment produces toxic amounts of free radicals, specifically superoxide, depleting glutathione reserves, causing DNA damage and PARP-1 over-activation as well as endoplasmic reticulum calcium release.

Pennsylvania State University, State College, PA Summer 1999

Undergraduate Research Program with Dr. John Golbeck

Rescue of a point mutation in the PSI protein PsaC with beta-mercaptoethanol as determined by Electron Paramagnetic Resonance (EPR).

Bacterially expressed, purified and reconstituted mutant PsaC protein. Analyzed the ability of mutant PsaC protein to function normally with beta-mercaptoethanol rescue via EPR.

Valparaiso University, Valparaiso, IN 1998-1999

Undergraduate Research Program with Dr. Thomas Goyne

Development of a biological technique for the production of lactones via E. coli. Compared the quality of lactones made from genetically modified E. coli to chemically synthesized lactones.

KATHRYN REINICKE, PAGE 2

Education And Training

Ph.D. in Biochemistry: Case Western Reserve University, Cleveland, OH December 2006

Bachelor of Science in Chemistry: Valparaiso University, Valparaiso, IN May 2000

Professional Skills and Techniques:

Cultivating mammalian cell cultures, cell growth assays, cell viability assays, colony formation assays, Hoechst staining for mycoplasma contamination

Western blotting, co-immunoprecipitation

Plasmid construct design, stable and transient transfection, RNA interference

Murine xenograft models and treatment (subcutaneous and oral gavage drug delivery)

DNA and RNA isolation, PCR, RT-PCR, agarose gel electrophoresis

TUNEL+ assay, cell cycle analyses by flow cytometry

Radioactive drug uptake, macromolecule binding assays

Comet assay for DNA damage

Teaching Experience

Mayo Clinic, Rochester, MN

Mentor, Summer Undergraduate Research Program, 2008-2010

• Cultivated the understanding of graduate-level science to college students through research projects and presentations

Mentor, High School Research Experience Program, 2010

• Guided high school students in their first exposure to scientific research through short-term research projects

Case Western Reserve University, Cleveland, OH

Mentor, High School Research Experience Program, 2004-2005

• Provided high school students exposure to scientific research through research projects and presentations

National Meeting Abstracts

M. Goetz, M. Kuffel, K.E. Reinicke, Z. Huang, A. Bode, J. Cheng, T. Hoskin, V. Suman, C. Arteaga, J. Reid, J. Hawse, and M. Ames. A Comparison of the Non-Genomic Effects of Endoxifen and Tamoxifen in Aromatase Inhibitor Resistant Breast Cancer: Differential Effects on the Estrogen Receptor Co-Regulator SRC3 (AIB1) and Identification of PKC and PI3K as Endoxifen Substrates. 36th Annual CTRC-AACR San Antonio Breast Cancer Symposium, 2013.

M. Goetz, X. Hou, V. Suman, K.E. Reinicke, M. Kuffel, P. Haluska, Jr., A. Oberg, D. Grill, J. Reid, A. Brodie, J. Ingle, and M. Ames. Endoxifen Exhibits Potent Anti-Tumor Activity and Regulates Different Genes Than Tamoxifen in an Aromatase Expressing MCF7 Model Resistant to Letrozole. 34th Annual CTRC-AACR San Antonio Breast Cancer Symposium, 2011. Poster Discussion I PD01-06.

K.E. Reinicke, X. Hou, M. Goetz, V. Suman, M. Kuffel, P. Haluska, J. Reid, M. Ames. Endoxifen exhibits potent in vitro and in vivo antitumor activity in ER+/HER2+ breast cancer and tamoxifen refractory tumors. AACR 102nd Annual Meeting, 2011. Abstract 2283.

M. Goetz, K.E. Reinicke, J. Reid, V. Suman, M. Kuffel, S. Safgren, S. Buhrow, C. Reynolds, R. Jenkins, J. Hawse, E. Perez, J. Ingle and M. Ames. Tamoxifen, HER2, and Endoxifen: The Role of CYP2D6 as a Predictor of Tamoxifen Resistance in ER+/HER2+ Breast Cancer. 32nd Annual CTRC-AACR San Antonio Breast Cancer Symposium, 2009. Abstract number 2006.

K.E. Reinicke, M. Kuffel, M. Goetz, M. Ames. Synergistic interactions between aminoflavone, Taxol and Camptothecin in human breast cancer cells. AACR 100th Annual Meeting, 2009. Abstract number 879.

K.E. Reinicke, D. Spitz, J. Pink, and D. Boothman. Reactive Oxygen Species Generation is Necessary, but not Sufficient, for beta-Lapachone-mediated Apoptosis. Metabolomics: From Bioenergetics to Apoptosis, Keystone Symposium, 2006.

K.E. Reinicke, M. Varnes, D. Spitz, J. Pink, and D. Boothman. Investigating the role of reactive oxygen species in beta-lapachone-mediated cell death. Department of Defense Era of Hope Breast Cancer Research Conference, 2005.

KATHRYN REINICKE, PAGE 3

Publications

Reid, J.M., Goetz, M.P., Buhrow, S.A., Walden, C., Safgren, S.L., Kuffel, M.J., Reinicke, K.E., Suman, V., Haluska, P., Hou, X., Ames, M.M. Pharmacokinetics of endoxifen and tamoxifen in female mice: implications for comparative in vivo activity studies. Cancer Chemother Pharmacol. 2014 Dec. 74(6):1271-8.

Cao, L., Li, L.S., Spruell, C., Xiao, L., Chakrabarti, G., Bey, E.A., Reinicke, K.E., Srougi, M.C., Moore, Z., Dong, Y., Vo, P., Kabbani, W., Yang, C.R., Wang, X., Fattah, F., Morales, J.C., Motea, E.A., Bornmann, W.G., Yordy, J.S., Boothman, D.A. Tumor-Selective, Futile Redox Cycle-Induced Bystander Effects Elicited by NQO1 Bioactivatable Radiosensitizing Drugs in Triple-Negative Breast Cancers. Antioxid Redox Signal. 2014 Jul 10. 21(2):237-50.

Bey, E.A., Reinicke, K.E., Srougi, M.C., Varnes, M., Anderson, V., Pink, J.J., Li, L.S., Patel, M., Cao, L., Moore, Z., Rommel, A., Boatman, M., Lewis, C., Euhus, D.M., Bornmann, W.G., Buchsbaum, D.J., Spitz, D.R., Gao, J., and Boothman, D.A. Catalase Abrogates beta-Lapachone-Induced PARP1 Hyperactivation-Directed Programmed Necrosis in NQO1-Positive Breast Cancers. Mol Cancer Ther. 2013. 12(10):2110-20.

Reinicke, K.E., Kuffel, M.J., Goetz, M.P., Ames, M.M. Synergistic interactions between aminoflavone, Paclitaxel and camptothecin in human breast cancer cells. Cancer Chemother Pharmacol. 2010; 66(3):575-83.

Bentle, M.S., Reinicke, K.E., Dong, Y., Bey, E.A., Boothman, D.A. Nonhomologous end joining is essential for cellular resistance to the novel antitumor agent beta-lapachone. Cancer Res. 2007; 67(14):6936-45.

Bey, E.A., Bentle, M.S., Reinicke, K.E., Dong, Y., Yang, C-R., Girard, L., Minna, J.D., Bornmann, W.G., Gao, J., Boothman, D.A. An NQO1- and PARP-1-mediated cell death pathway induced in non-small cell lung cancer cells by beta-lapachone. Proc Natl Acad Sci USA. 2007; 104(28):11832-7.

Bey, E.A., Wuerzberger-Davis, S.M., Pink, J.J., Yang, C-R., Araki, S., Reinicke, K.E., Bentle, M.S., Dong, Y., Cataldo, E., Criswell, T.L., Wagner, M.W., Li, L., Gao, J., Boothman, D.A. Mornings with Art, lessons learned: feedback regulation, restriction threshold biology, and redundancy govern molecular stress responses. J Cell Physiol. 2006; 209(3):604-10.

Bentle, M.C., Reinicke, K.E., Bey, E.A., Spitz, D.R., Boothman, D.A. Calcium-dependent modulation of poly(ADP-Ribose) polymerase-1 alters cellular metabolism and DNA repair. J Biol Chem. 2006; 281(44):33684-96.

Bentle, M.C., Bey, E.A., Dong, Y., Reinicke, K.E., Boothman, D.A. New tricks for old drugs: the anticarcinogenic potential of DNA repair inhibitors. J Mol Histol. 2006; 37(5-7):203-18.

Araki, S., Israel, S.L., Leskov, K.S., Criswell, T.L., Mayo, L.D., Beman, M., Klokov, D.Y., Reinicke, K.E., Cataldo, E., Boothman, D.A. Clusterin proteins: Stress-inducible polypeptides with proposed functions in multiple organ dysfunction. Brit J Radiology Suppl. 2005; 27:106-13.

Reinicke, K.E., Bey, E.A., Bentle, M.S., Pink, J.J., Ingalls, S.T., Hoppel, C.L., Misico, R.I., Arzac, G.M., Burton, G., Bornmann, W.G., Sutton, D., Gao, J., Boothman, D.A. Development of beta-lapachone prodrugs for therapy against human cancer cells with elevated NAD(P)H:quinone oxidoreductase 1 levels. Clin Cancer Res. 2005;11(8):3055-64.

Tagliarino, C., Pink, J.J., Reinicke, K.E., Simmers, S.M., Boothman, D.A. mu-Calpain activation in beta-lapachone-mediated apoptosis. Cancer Biol Ther. 2003;2(2):141-52.

Volunteering

Metro North Adult Basic Education, Brooklyn Center, MN

Tutor, Adult Basic Education, GED Brush-up, December 2013-Present

• Assists students with coursework towards earning their GED, assists teachers with classroom instruction, teaches math with a focus on algebra

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