SHRISHTI

SINGH

** ****** ******, *** **, Stamford, CT: 06905 +1-203-***-**** ac0lz8@r.postjobfree.com Overview

To create new materials and utilize them in the health sector by using my scientific skills, critical thinking and creative articulation.

Education

Masters in Biomedical Engineering (GPA 4.0) Fall 2015-Spring 2017 University of Bridgeport, Bridgeport, CT, USA

Major: Biomaterial Science and Engineering, Bioelectronics, Tissue Engineering, Fabrication of nanocomposites.

Minor: Magneto Bio-engineering, Quantitative NMR and MRI, Instrumental Analysis of Nanomaterials, Bio-computational Modeling.

Bachelor in Engineering in Biotechnology (GPA 3.9) Fall 2011-Spring 2015 M S Ramaiah Institute of Technology, Bangalore, Karnataka, India Major: Cell and Molecular Biology, Human Physiology, Human Anatomy, Genetic Engineering, Biochemistry, Tissue Culture, Biomedical Instrumentation Maintenance, Service and Safety, Electric and Electronics.

Minor: Chemical Thermodynamics, Fluid Flow and its Dynamics, Bio-reactions and its Kinetics Thesis

Development of chip-based biosensor for detection of hereditary non- polypylosis colorectal cancer (Lynch Syndrome) marker Fall 2015-Spring 2017 University of Bridgeport, Bridgeport, CT

Summary: A chip based system is created for the detection of mismatch repair proteins at nanomolar concentrations whose deficiency leads to Lynch Syndrome, which affects 5% of world’s population.

Key Responsibilities: Development and optimization of the conductive graphene-polypyrrole substrate used in the biosensor using cyclic voltammetry, validation of nanomolar 2

concentration detection of the biosensor using electrochemical impedance spectroscopy and simulation of the interactions between the analyte and substrate. Funding: Department of Public Health, CT.

Study of interactions between tri-peptides and carbon nanotubes(CNT) to develop a method for sorting CNT based on their chirality Fall 2015-Spring 2017 University of Bridgeport, Bridgeport, CT

Summary: Interactions between peptides and nanotubes has not been studied extensively. Such studies are important because peptides are the building blocks of proteins. Knowing such interactions can lead to the development of more sensitive sensors composed of CNT. In this study, tripeptides are taken from flagellin protein and modelled to study their interactions with different forms of CNT. Modelling studies and UV-Visible studies are carried out. Key Responsibilities: Development of procedures to synthesize and validate the tripeptides, developing a protocol to establish the interaction between the peptides and CNTs, validating the type of CNTs obtained and their chiralities using Circular Dichroism, statistical modeling of the type of interactions.

Synthesis and characterization of silver nanoparticles derived from Cinnamon zeylanicum extract and it’s inhibitory effect on E.fecalis, a bacterium found in oral cavities. Winter 2014-Spring 2015

M S Ramaiah Institute of Technology, Bangalore, Karnataka Summary: Preparation of silver nanoparticles from Cinnamon (a common spice) bark, characterization of the particles and testing their efficacy against E.fecalis and breast cancer cell lines.

Key Responsibilities: Developing a protocol for the synthesis of silver nanoparticles, their characterization using UV-Visible spectroscopy, SEM and XRD, testing their efficacy using anti- bacterial and MTT assays.

Funding: Dr. Chandraprapha MN Lab, M S Ramiah Institute of Technology Projects

Analysis of the effect of low intensity pulsed ultrasound on glucose intake through the cell membrane for higher vaccine production Summer 2016 University of Bridgeport, Bridgeport, CT

Summary: Using simulation software Visual Molecular Dynamics to demonstrate the amount of force required for the passage of glucose through the cell membrane. 3

Key Responsibilities: Creating the simulation system using tcl scripting and quantitative and statistical analysis of the data obtained.

Inhibition of the activity of dinucleotide phosphate kinase by graphene oxide and its implications in cardiovascular diseases Winter 2016 University of Bridgeport, Bridgeport, CT

Summary: Using simulation software Visual Molecular Dynamics to demonstrate the loss of enzyme activity and competitive inhibition of the enzyme activity by introducing graphene oxide.

Key Responsibilities: Creating the simulation system using tcl scripting and quantitative and statistical analysis of the data obtained, demonstrating the effect of inhibitors on the enzyme activity and mathematically modeling the type of inhibition. Funding: National Science Foundation (NSF)

Experience

University of Bridgeport

Graduate Research Assistant Fall 2015-Spring 2017

Key Responsibilities: Synthesis of graphene and carbon nanotube polymer based nanocomposites chemically and electrochemically using cyclic voltammetry, measure of their electron transfer properties using Electrochemical Impedance Spectroscopy, electrospinning of these polymers to generate scaffolds for cardiomyocytes growth, characterization of these substrates using SEM, TEM and AFM. Additional Information: All training for SEM, TEM and AFM has been received at the Brookhaven National Laboratory, Upton, NY as part of a joint proposal between University of Bridgeport and the US Department of Energy run Brookhaven Lab. Vellore Institute of Technology, Tamil Nadu, India Research Intern Summer 2014

Key Responsibilities: Study of toxicity of silver nanoparticles on algal cells by using MTT assay and fluorescence microscopy.

Publications

Macwan, I, Singh, S. et al. Interactions between avidin and graphene for development of a biosensing platform. Biosens. Bioelectron. 89, Part 1, 326–333 4

(2017). Imapct Factor of Biosensors and Bioelectronics: 7.47 Summary: The work details how the interaction between avidin and graphene (substrate) can be quantified using Electrochemical Impedance Spectroscopy hence providing a new class of electrical sensors which can measure a change in analyte concentration as low as nanomolar when deposited on the substrate.

Increasing Vaccine Production Using Pulsed Ultrasound Waves, IEEE Journal of Translational Engineering in Health and Medicine (Under Review) Summary: The work highlights the use of pulsed ultrasound waves to increase the uptake of glucose by the cells hence resulting in increased vaccine production. Effects of carbon nanotubes in photo anode, tin sulphide in carbon electrode and titania in electrolyte on quantum dot solar cell performance, The Journal of Physical Chemistry C (Under Review)

Poster Presentations

1. Singh S, Kalluri A, Macwan I and Patra P, “Single step synthesis of p-aminobenzoic acid rhombohedral shaped crystals using graphene nanoplatelets”; Biomedical Engineering Society Annual Conference 2016, Minneapolis, MN.

2. Singh S, Macwan I and Patra P, “Chirality based separation of Sw-CNT using AMB-1 derived flagellin tripeptides: a mathematical study”, Material Research Society Annual Meeting 2016, Boston, MA.

3. Singh S, Khan MD, Macwan I, Liu J, Hingorani M and Patra P, “A novel biosensor for the detection of cancer biomarkers”, Annual IEEE Connecticut Conference on Industrial Electronics, Technology and Automation 2016, Bridgeport, CT. 4. Singh S, Macwan I and Patra P, “Chirality based separation of Sw-CNT using AMB-1 derived flagellin tripeptides: a mathematical study”, American Society for Engineering Education Northeast Conference 2016, Kingston, RI. Awards and Acknowledgements

Member of Phi Kappa Phi since Spring 2017

Best Bachelor in Engineering Project 2015

Awarded by M S Ramaiah Institute of Technology Alumni Association 5

Winner of CamTECH India Jugadathon 2015

Awarded by MERCK for Mother’s. In association with Massachusetts Institute of Technology, Harvard Medical School and Massachusetts General Hospital. Sponsored by GE Health. Description: An international hackathon competition for innovation of economical healthcare technologies. MERCK for Mother’s challenge was to generate a solution to determine maternal and infant sepsis at early stages, hence the technology MOM (Maternity Over Morality) was born as an idea which beat out hundreds from institutes like MIT, Stanford and Harvard. MOM made use of sturdy nanofilms to detect the bacterial species responsible for maternal sepsis.

Certifications

Forensic Science by International Open Academy August 2016 Criminology and Profiling by International Open Academy Aug 2016 Academic Accomplishment Award 2017

Awarded by School of Engineering, University of Bridgeport Best Poster Award Category- Graduate Honorable Mention 2016 Awarded by 2016 American Society for Engineering Education, ASEE 2016, Northeast Conference, University of Rhode Island.

Best Poster Award Master Category Third Place 2016 Awarded by Annual IEEE Connecticut Conference on Industrial Electronics, Technology and Automation, CT-IETA 2016, University of Bridgeport. Best Poster Award Master Category Honorable Mention 2016 Awarded by Annual IEEE Connecticut Conference on Industrial Electronics, Technology and Automation, CT-IETA 2016, University of Bridgeport. 6

Best Poster Award Graduate Category First Place 2017 Awarded by Faculty Research Day, University of Bridgeport, FRD 2017, University of Bridgeport. Skills

Well-versed to work on SEM, TEM, AFM, FT-IR, STM, UV-Visible Spectrophotometer, Fluorescence microscope, EIS, CV, Electrospinning, Origin 3.0 and above, Visual Molecular Dynamics for simulation and modeling, Tcl scripting for Visual Molecular Dynamics. Reference

Dr. Prabir Patra Dr. Michael Gargano

Head and Professor CEO and Director

Department of Biomedical Engineering St. Vincent’s Medical Center University of Bridgeport, Bridgeport, CT 06604 Bridgeport, CT ac0lz8@r.postjobfree.com ac0lz8@r.postjobfree.com

+1-203-***-****

Contact this candidate