Hyeong Hun Choe

**** ******** **, ****** *****, CA 94598

Phone: 319-***-**** Email: ac0kp5@r.postjobfree.com

Summary

Organized research assistant specialized in molecular biology, tissue engineering, and gene therapy. Latest research includes focus on chondrogenic progenitor cells (CPCs) and Proteoglycan 4 / lubricin (PRG4) gene therapy for preventing osteoarthritis.

Highlight / Skills

Molecular biology techniques

DNA isolation

In-vitro transcription

Microbiological techniques

Gene cloning, PCR

Cell culture

Western blotting

Protein analysis

HPLC

FACS

Confocal imaging

Immunostaining/Immunohistochemstry

Biostatistics

Design of biomaterial and In vivo test

Accomplishments

Completed advanced research on Adeno-Associated Virus-mediated PRG4 Gene Therapy.

Characterization of Chondrogenic progenitor cells which respond to cartilage injury. (Arthritis & Rheumatism, 64(11), 3626-37, 2012 (SCI, IF; 7.866, selected article from F1000 faculty))

Experience

Research assistant, December 2008 to January 2016

The University of Iowa – Iowa City, IA

Served as a laboratory research assistant for 6 years at the Cell Biology Lab in the Department of Orthopaedics and Rehabilitation at the University of Iowa.

Research assistant, August 2007 to July 2008

Ajou University – Suwon, South Korea

Served as a Research Assistant for 1 year at the Biochemistry lab in the Bioengineering Department at the Ajou University.

Education

Ph.D. : Biomedical Engineering, 2015

The University of Iowa – Iowa City, IA, USA

Thesis: Feasibility of intra-articular Adeno-Associated Virus-mediated proteoglycan-4 gene therapy to prevent

osteoarthritis (Advisor: Prof. James A. Martin & Prof. Tae-Hong Lim)

Master of Science : Bioengineering, 2007

Ajou University – Suwon, South Korea

Thesis: Identification and Characterization of a Novel Oligomeric Decaprenyl Diphosphate Synthase

(Advisor: Prof. Yeon-Woo Ryu)

Bachelor of Science : Bioengineering, 2005

Ajou University – Suwon, South Korea

Publications

1. Choe Hyeong H, Hwang Heejin, Kim Seung B, Yoon Sangyoung, Jo Do Hyun, Ryu Yeonwoo, Kim T. Doohun, “Identification and Characterization of a Novel Oligomeric Decaprenyl Diphosphate Synthase”, Protein and Peptide Letters, 16(9), 1036-1040, 2009

2. Heejin Hwang, Hyunghun Choi, Hyun-kyung Kim, Do Hyun Jo, T. Doohun Kim, “Ionic liquids promote amyloid formation from α-synuclein”, Analytical Biochemistry, 386(2), 293–295, 2009

3. Dongrim Seol, Hyeong Hun Choe, Hongjun Zheng, Keewoong Jang, Prem S Ramakrishnan, Tae-Hong Lim and James A Martin, “Selection of reference genes for normalization of quantitative real-time PCR in organ culture of the rat and rabbit intervertebral disc”, BMC Research Notes, 4(162), 2011

4. Dongrim Seol, Daniel J. McCabe, Hyeong Hun Choe, Hongjun Zheng, Yin Yu, Keewoong Jang, Morgan W. Walter, Abigail D. Lehman, Lei Ding, Joseph A. Buckwalter, James A. Martin, “Chondrogenic progenitor cells respond to cartilage injury”, Arthritis & Rheumatism, 64(11), 3626-37, 2012 (SCI, IF;7.866, selected article from F1000 faculty)

5. Dongrim Seol, Hyeong Hun Choe, Prem S. Ramakrishnan, Keewoong Jang, Gail L. Kurriger, Hongjun Zheng, Tae-Hong Lim, James A. Martin, “Organ culture stability of the intervertebral disc: rat versus rabbit”, Journal of Orthopaedic Research, 31(6), 838-46, 2013

6. Dongrim Seol, Michael J. Magnetta, Prem S. Ramakrishnan, Gail L. Kurriger, Hyeong Hun Choe, Keewoong Jang, James A. Martin, Tae-Hong Lim, “Biocompatibility and Preclinical Feasibility Tests of a Temperature-Sensitive Hydrogel for the Purpose of Surgical Wound Pain Control and Cartilage Repair”, JBMR Part B, 101(8), 1508-15, 2013

7. Dongrim Seol, Yin Yu, Hyeong Hun Choe, Keewoong Jang, Marc J. Brouillette, Hongjun Zheng, Tae-Hong Lim, Joseph A. Bcukwalter, James A. Martin, “Effect of short-term enzymatic treatment on cell migration and cartilage regeneration; In vitro organ culture of bovine articular cartilage”, Tissue Engineering Part A, 20(13-14), 1807-14, 2014

8. Hyeong Hun Choe, Hongjun Zheng, Dongrim Seol, Prem S. Ramakrishnan, Marc J. Brouillette, James A. Martin, “Feasibility of Intra-articular Adeno-Associated Virus-mediated PRG4 Gene Therapy”, 2015 (processing)

Abstracts

1. Hyeong Hun Choe, Gi-Sub Choi, Jin-Ho Seo, and Yeon-Woo Ryu. “Ops gene knock-out and dps gene expression for coenzyme Q10 production in E. coli BW25113", 2005 The Korean Society for Biotechnology and Bioengineering, Jinju City, Korea, p293, October 27-29, 2005

2. Hyeong Hun Choe, Gi-Sub Choi, Jin-Ho Seo, and Yeon-Woo Ryu. “Ops gene knockout system construction and Dps gene expression for coenzyme Q10 production in E. coli BW25113", Genomics and Proteomics in Health and Agriculture, Program and Abstract of 18th FAOBMB Symposium, Lahore, Pakistan, p125, November 20-23, 2005

3. Hyeong Hun Choe, Seung-Bum Kim and Yeon-Woo Ryu. “Dps gene expression and analysis of coenzyme Q10 production by using pQE30 expression vector in E. coli" 2006 The Korean Society for Biotechnology and Bioengineering, Chungju, Korea, p174, May 10-12, 2006

4. Hyeong Hun Choe, Yeon Woo Ryu, and Jin Ho Seo. “Dps gene expression and analysis for coenzyme Q10 production by using pQE30 vector in E. coli” 10th International Symposium on the Genetics of Industrial Microorganisms, Prague, Czech Republic, p20, June 24-28, 2006

5. Seol DR, Choe HH, Lehman AD, Homandberg G, Heiner AD, Ramakrishnan PS, Lim TH, Martin JA, “Effect of impact-induced medium in bovine articular cartilage on surrounding tissues: Cell proliferation and migration study”, 2009 World Congress on Osteoarthritis, Montreal, Quebec, Canada, September 10-13, 2009

6. McCabe Daniel J, Seol Dongrim, Choe Hyeong Hun, Stroud N, Buckwalter J, Martin James, “Do Cartilage-derived Stem Cells Repopulate Dead Zones in Injured Cartilage?”, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, LA, p949, March 6-9, 2010

7. Choe HH, Seol DR, Jang KW, Lehman AD, Homandberg G, Heiner AD, Ramakrishnan PS, Lim TH, Martin JA, “Effect of Impact-induced chemical factors on bovine articular cartilage: a cell proliferation and migration study”, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, LA, March 6-9, 2010

8. Choe HH, Seol DR, Lehman AD, Homandberg G, Heiner AD, Ramakrishnan PS, Lim TH, Martin JA, “Effect of impact-induced chemical factors on bovine articular cartilage: a cell proliferation and migration study”, KSEM Midwest Regional Conference, Iowa city, IA, March 20-21, 2010

9. Seol Dongrim, Choe Hyeong Hun, McCabe Daniel J, Stroud N, Buckwalter J, Lim Tae-Hong, Martin JA, “Articular cartilage regeneration using cartilage fragments with fibrin hydrogel”, KSEM Midwest Regional Conference, Iowa city, IA, March 20-21, 2010

10. Choe HH, Seol DR, Lehman AD, Heiner AD, Ramakrishnan PS, Lim TH, Martin JA, “Effect of chemokines on migratory progenitor cell (MPC) migration”, 2011 Annual Meeting of the Orthopaedic Research Society, Long Beach, CA, January 13-16, 2011

11. Seol DR, Choe HH, Zheng HJ, Jang KW, Ramakrishnan PS, Lim TH, Martin JA, “Selection of reference genes for normalization of quantitative real-time PCR in organ culture of the rat and rabbit intervertebral disc”, 2011 Annual Meeting of the Orthopaedic Research Society, Long Beach, CA, January 13-16, 2011

12. Seol DR, Choe HH, Kurriger GL, Jang KW, Ramakrishnan PS, Lim TH, Martin JA, “Intervertebral disc stability in organ culture: rat versus rabbit”, 2011 Annual Meeting of the Orthopaedic Research Society, Long Beach, CA, January 13-16, 2011

13. Choe HH, Zheng H, Seol DR, Martin JA, “HMGB1, a key activator of chondrogenic progenitor cells and a possible trigger of post trauma osteoarthritis”, 2012 Annual Meeting of the Orthopaedic Research Society, San Francisco, CA, p464, February 4-7, 2012

14. Choe HH, Zheng H, Ramakrishnan PS, Seol DR, Martin JA, “Lubricin is an important glycoprotein for preventing progress of post-traumatic osteoarthritis”, 2014 Annual Meeting of the Orthopaedic Research Society, New Orleans, LA, March 15-18, 2014

15. Seol D, Zhou C, Yu Y, Choe HH, Lehman AD, Jang K, Zheng H, Martin JA, “Characteristics of migratory meniscus progenitor cells (MPCs) in meniscus injury”, 2014 Annual Meeting of the Orthopaedic Research Society, New Orleans, LA, March 15-18, 2014

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