Rand S. Schwartz

*** ********** **** #** 302-***-****

Wilmington, Delaware 19803 ac0hqy@r.postjobfree.com

www.linkedin.com/pub/rand-schwartz/42/3b2/665/

PROFESSIONAL PROFILE

Versatile biochemist with pharmaceutical drug discovery and agrochemical industrial research and development experience. Expertise pursuing targets for crop disease resistance and cardiovascular disease in humans. Research experience encompasses in vitro assay development for target discovery and elucidation of chemical mechanisms. Recognized for contributions on multidisciplinary cardiovascular drug discovery team for establishing assays elucidating potency and mechanism of action for compounds of interest. Contributed to the characterization of a lipase inhibitor currently in phase III clinical trials for the treatment and prevention of atherosclerosis. Highly interactive problem-solver with expertise in clear communication and for building effective working relationships with diverse employee groups.

PROFESSIONAL EXPERIENCE

Agilent Technologies, Inc., Wilmington, DE May 2011 – December 2016

Applications Engineer

I provided applications and technical support to Agilent’s customers, our sales team, field service engineers, distributers, etc., for gas chromatography.

Merck & Co., Inc., NewAgeSys, Inc., West Point, PA October 2009 - April 2010

Biologist II -Bioanalytics & Pathology

Designed, developed, and analytically validated immunoassays for biomarker and immunogenicity evaluation.

Assayed thousands of ELISA’s to support pre-clinical trials in Neuroscience.

Developed efficient sample clean-up strategies to prep serum, plasma and CSF samples for HPLC analysis.

JRF America - a GLP compliant-contract research organization. Judge Technical Staffing,

King of Prussia, PA October 2008 - January 2009

Analytical Chemist

Performed trace level analysis of organic compounds in complex environmental matrices. Conducted

14C-environmental-fate studies for pesticide registration with the EPA, DEP and the OECD.

GlaxoSmithKline Pharmaceuticals, Cardiovascular Biochemistry, King of Prussia, PA

July 2006 - August 2008

Scientist

Established fluorescent enzymatic lipase assays and characterized high throughput screening (HTS) hits for IC50’s, time dependent behavior, structure-activity relationships (SAR), reversibility rates, mechanism of inhibition and mode of action.

Designed and executed in vitro assays for cardiovascular targets of interest and reported results to chemists.

Contributed to the characterization of Darapladib, currently in phase III clinical trials for the treatment and prevention of atherosclerosis.

Techniques included: Fluorescence and UV/Vis assays in a 384 well format, methods development for HPLC-MS, programming and operation of automation for liquid handling.

Reported Ki, time dependence and reversibility results to medicinal chemistry to develop structure activity relationships (SAR) and to drive go/no go decisions.

PAGE TWO RAND S. SCHWARTZ

E.I. DuPont De Nemours & Company, Wilmington, DE 1992-2006

Associate Biochemist II, Pioneer/DuPont Biotech, Crop Genetics R&D 2001-2006

Developed better tasting soy-products with added functionality that is more acceptable to the general western consumer.

Utilized analytical biochemistry, steady-state enzyme assays, and gas chromatography (GC/FID) for fatty acid analysis as a member of a multidisciplinary team determining the chemical basis of soy flavor.

Designed and implemented assays to screen transgenic soy embryos and seeds for desired phenotype.

Designed biochemical screens for analyzing tens of thousands of soy embryos and seeds, which resulted in a 50-80% reduction in time, resources and cost.

The result went to product, SUPRO ® XF, a soy protein isolate sold to enhance the flavor and functionality for ready-to-drink and powder beverages.

Associate Biochemist, DuPont AgBiotech, Nutrition & Health/Disease Resistance 1999-2001

Identified and isolated novel peptides, proteins and secondary metabolites involved in resistance to fungal infection in wheat by Septoria tritici.

Techniques included: proteomics, analytical biochemistry, sub-cellular fractionation, isolation and collection of intercellular apoplastic fluid, bioassays, steady-state enzyme assays, capillary LC/MS/MS, MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization-Time of Flight) mass spectroscopy, database searches and bioinformatics.

Discovered novel proteins present in the intercellular apoplastic fluid from a cultivar of wheat resistant to infection by Septoria tritici but absent in the susceptible cultivar of wheat.

Associate Biochemist, DuPont Agricultural Crop Protection Products 1992-1999

Worked as part of a multidisciplinary team to discover novel herbicide and fungicide chemical leads.

Optimized chemical leads using a rational inhibitor design approach for fungicide discovery.

Techniques included: protein purification and characterization, refolding of insoluble recombinant proteins, western blot, SDS-PAGE, assay development, design and implementation of steady-state enzyme assays and medium throughput screens.

Screened compounds synthesized by chemists and assembled structure-activity relationships (SAR) to advance chemical lead discovery.

Published Accomplishments

Utilized the crystal structure of scytalone dehydratase to elucidate the residues involved in catalysis and binding of substrate. Site directed mutagenesis was used to alter these residues and the effects were studied by enzyme kinetic analysis, (see addendum).

Purified and refolded insoluble recombinant proteins and determined kinetic constants for wild type and 15 site directed mutants. The goal was to understand the chemical mechanism of scytalone dehydratase to advance our rational inhibitor design program.

EDUCATION AND PROFESSIONAL AFFILIATIONS

Graduate Studies University of Delaware, Department of Chemistry, Newark, DE 1989-1993

Continuing Education Non-Degree Graduate program. Earned 15 graduate credit hours while working full time. Course work included: Advanced Physical Organic Chemistry, Instrumental Methods, and four semesters of Biochemistry.

B.A., Biology Rutgers, The State University of New Jersey 1989

Active member of the Delaware Valley Enzymology Club.

Active member of the American Chemical Society’s Chemistry Subdivision.

PAGE THREE ADDENDUM RAND S. SCHWARTZ

Publications:

Basarab, G. S., Jordan, D. B., Gehret, T. C., and Schwartz, R. S. (2002) Design of inhibitors of scytalone dehydratase: probing the interactions with an asparagine carboxamide. Bioorg. Med. Chem. 10, 4143-4154.

Basarab, G. S., Jordan, D. B., Gehret, T. C., Schwartz, R. S., Bonman, J. M., and Smith, G. S. (2002) Design of scytalone dehydratase inhibiting rice blast fungicides. Synthesis and Chemistry of Agrochemicals VI. American Chemical Society, Washington, DC, pp. 278-291.

Jordan, D. B., Basarab, G. S., Steffens, J. J., Schwartz, R. S., and Doughty, J. G. (2000) Tight binding inhibitors of scytalone dehydratase: effects of site-directed mutations. Biochemistry 39, 8593-8602.

Jennings, L. D., Rayner, D. R., Jordan, D. J., Okonya, J. F., Basarab, G. S., Amorose, D. K., Anaclerio, B. M., Lee, J. K., Schwartz, R. S., and Whitmore, K. A. (2000) Cyclobutane carboxamide inhibitors of fungal melanin: biosynthesis and their evaluation as fungicides. Bioorg. Med. Chem. 8, 897-907.

Jennings, L. D., Wawrzak, Z., Amorose, D., Schwartz, R. S., and Jordan, D. B. (1999) A new potent inhibitor of fungal melanin biosynthesis identified through combinatorial chemistry. Bioorg. Med. Chem. Lett. 9, 2509-2514.

Jordan, D. B., Kranis, K. T., Picollelli, M. A., Schwartz, R. S., Sternberg, J. A., and Sun, K. M. (1999) Famoxadone and oxazolidinones: potent inhibitors of cytochrome bc1. Biochem. Soc. Trans. 27, 577-580.

Basarab, G. S., Jordan, D. B., Gehret, T. C., Schwartz, R. S., and Wawrzak, Z. (1999) Design of scytalone dehydratase inhibitors as rice blast fungicides: derivatives of norephedrine. Bioorg. Med. Chem. Lett. 9, 1613-1618.

Jordan, D. B., Lessen, T. A., Wawrzak, Z., Bisaha, J. J., Gehret, T. C., Hansen, S. L., Schwartz, R. S., and Basarab, G. S. (1999) Design of scytalone dehydratase inhibitors as rice blast fungicides:

(N-phenoxypropyl)-carboxamides. Bioorg. Med. Chem. Lett. 9, 1607-1612.

Basarab, G. S., Steffens, J. J., Wawrzak, Z., Schwartz, R. S., Lundqvist, T., and Jordan, D. B. (1999) Catalytic mechanism of scytalone dehydratase: site-directed mutagenesis, kinetic isotope effects, and alternate substrates. Biochemistry 38, 6012-6024.

Jordan, D. B., Basarab, G. S., Steffens, J. J., Lundqvist, T., Pfrogner, B. R., Schwartz, R. S., and Wawrzak, Z. (1999) Catalytic mechanism of scytalone dehydratase from Magnaporthe grisea. Pestic. Sci. 55, 277-280.

Steffens, J. J., Basarab, G. S., Schwartz, R. S., Lundqvist, T., Wawrzak, Z., and Jordan, D. B. (1999) Substrate and inhibitor binding by scytalone dehydratase, a target of rice blast fungicides. Modern Fungicides and Antifungal Compounds II (Lyr, H., Russell, P. E., Dehne, H. W., and Sisler, H. D. eds.), 121-130. Intercept Ltd, Andover, Hampshire, UK.

Jordan, D. B., Livingston, R. S., Bisaha, J. J., Duncan, K. E., Pember, S. O., Picollelli, M. A., Schwartz, R. S., Sternberg, J. A., and Tang, X.S. (1999) Oxazolidinones: a new chemical class of fungicides and inhibitors of mitochondrial cytochrome bc1 function. Pestic. Sci. 55, 213-215.

Jordan, D. B., Livingston, R. S., Bisaha, J. J., Duncan, K. E., Pember, S. O., Picollelli, M. A., Schwartz, R. S., Sternberg, J. A., and Tang, X.S. (1999) Mode of action of famoxadone. Pestic. Sci. 55, 105-118.

Jordan, D., Viitanen, P., Wawrzak, Z., Picollelli, M., Bacot, K., Schwartz, R., and Thompson, J. (1997) Cloning, overexpression, crystallization, and some kinetic studies on E. coli riboflavin synthase. Flavins and Flavoproteins 1996 (Stevenson, K. J., Massey, V., and Williams, C. H. eds.), 945-948. University of Calgary Press, Calgary, Canada.

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