GEETA L. IYER, Professional Science Masters in Biotechnology

Tampa, FL 33613 813-***-**** ac0hbb@r.postjobfree.com https://www.linkedin.com/in/geetaliyer PROFESSIONAL EXPERIENCE

Research Technician- (USF Health Byrd Alzheimer's Institute, Tampa, FL) August 2016- present

Project 1: Assay optimization to check if the test drugs can reduce the α-synuclein aggregated protein using -Thioflavin-T assay, Stressmarq proteasomal aggregates, MTS assay, LDH assay, 6-ODHA, Alamar Blue Asssay

Making presentations and scheduling monthly meeting with collaborators to give updates on the project and decide the future directions

Project 2: Determined whether the test Drug WA affects α-synuclein levels and the degradation pathway involved in the clearance of α-synuclein after WA treatment

Demonstrated the localization of α-synuclein in cells by immunofluorescence

Investigated the translocation of α-synuclein using immunoprecipitation analysis followed by Western blotting

Designed experiments, problem solved and analyzed data for future publications

Supervised several undergraduates by organizing responsibilities, designing and coordinating experiments, training them in laboratory techniques, instrumentation, procedures, and daily tasks

Mice handling wet lab experience-Estrus detection, Weaning and sexing mice, Mouse blood collection, Injection routes (SC, IP, PO), Blood collection

(Submandibular, Saphenous, Femoral, Cardiac), Anesthesia/Analgesia, Euthanasia

Was selected as the top 10 candidates from USF across all disciplines for virtual heat round of 3-minute thesis presentation competition Teaching Assistant – (USF College of Medicine, MS in Biotechnology) August 2016- December 2016

Instructed a graduate lab course -Methods in Molecular Biology (BCH 6135C) of ~30 students throughout each week by designing lectures, leading discussions, demonstrating procedures, fielding questions, grading student lab reports and presentations. Research Intern - (USF Health Byrd Alzheimer's Institute, Tampa, FL) October 2015 – August 2016

Annotation of cell cycle pathway affected by fendiline, a potential drug to treat pancreatic cancer and further study the process using cell lines transfected with knockdown of certain proteins that play an important role

Demonstrated the effect of combinatorial drug treatment and determined the effective concentration on different pancreatic cancer cell lines followed by comparison of changes in protein expression to human pancreatic tissue samples

Verified the effect of combinatorial drugs that influence metastatic properties of cells

Interpretation of protein localization due to endocytic trafficking proteins under the influence of fendiline by subcellular fractionation

Delivered periodic presentations of results and work in progress to the Principal Investigator and maintained lab notebook

Conducted trainings and mentored two undergraduate students from USF Research Assistant (Fine Fragrances Private Limited (FFPL), Mumbai, India) March 2015- July 2015

Designed the experiment for enhanced production of essentials oil from patchouli using tissue culture leading to a decrease in manufacturing cost by 10% and increase in production of oil by 15%

Responsible for the batch production of patchouli using tissue culture

Checked different parameters for setting up soilless gardening for producing microgreen

Identified different spent products from oil manufacturing companies for production of different mushrooms

Documented all the procedures and the data obtained ACADEMIC PROJECTS

Research Intern (Advanced Centre for Treatment Research and Education in Cancer, India) September 2013-March 2014

Title of Project: Investigated the role of glycosylation and phosphorylation of Keratin 18 in regulating its properties

Generated HHL17 cells stably expressing K18 O-GlcNAc (WT, S29A and S48A) and phospho mutant (S52A). Validated the cell line stably expressing K18 WT and K18 O-GlcNAc mutants in HHL17 cells using fluorescence detection of YFP tagged K18 and confirmed the presence of K18 O-GlcNAc mutants by PCR sequencing

Confirmed the HHL17 cell lines stably expressing K18 O-GlcNAc mutants were mycoplasma free using PCR

Investigated which K18 O-GlcNAcylation site regulates its stability using O-GlcNAc mutants of K18

Examined which K18 O-GlcNAcylation site regulates phosphorylation at S33 and S52 in K18 O-GlcNAcylation double mutants

Standardized anti-K8 antibody to study filament architecture by indirect immunofluorescence

Presented, organized and developed data, which was present in lab meetings on weekly basis

A copy of thesis and presentation was submitted to University of Mumbai

The work done was acknowledged in “Functional implications of O-GlcNAcylation dependent phosphorylation at proximal site on keratin 18”. EDUCATION

University of South Florida

Master of Science in Biotechnology (GPA:3.8/4) December 2016

S.I.E.S College, Sion, India

Master of Science, Biotechnology (GPA:4.0/4.0) August 2014

Jai Hind College, Churchgate, India

Bachelor of Science, Biotechnology (GPA:3.51/4.0) May 2012 TECHNICAL SKILLS

Cell Biology: Mammalian Cell Culture, Flow Cytometry, Immunofluorescence Molecular Biology: PCR, qPCR, RT-PCR, DNA/RNA Isolation, Restriction Enzyme Analysis, ELISA Protein Biology: Protein Purification, SDS-PAGE, Western Blotting, Nuclear and cytoplasmic fractionation, Immunoprecipitation (IP) Genetics: Site-Directed Mutagenesis, DNA Cloning (Transformation of mammalian cells and E.coli, transduction of expression vector in cell line using lentivirus), DNA Quantification, Primer Design

Computer: GraphPad, Stat View, Python, Image J, Photoshop, AlphaEase FC, Database querying namely Pubmed, Entrez, PDB, homology searching and other sequence comparisons.

Instrumentation- Spectrophotometer UV-VIS, Microscopy-Confocal and Fluorescent, phase contrast microscopy; Nanodrop, Sonicator, Ultra centrifuge, Microtome. CERTIFICATIONS

Perusing a certificate course for Lean six sigma green belt.

AALAS- Aseptic Technique in Rodent Survival Surgical Procedures, Handling and Use of Immune Deficient Mice

Contact this candidate