Research Volunteer at NYU Langone Medical center

Snigdha Pramanik

*** ******* *****, *********, ** 94089 347-***-**** ******@***.***

EDUCATION

New York University, School of Engineering, New York, USA May’17 Master of Science in Biotechnology - GPA: 3.5/4

Major Courses: Advanced Cell and Molecular Biology, Biosensors, Genetic Engineering, Biotechnology and pharmaceutical industry

Manipal University, Manipal, India May’14

Bachelor of Engineering in Biotechnology - GPA: 3.23/4 Major Courses: Microbiology, Immunology, Plant Biotechnology, Biochemistry, Downstream Processing, Bio-Chemistry

SKILLS

• Research: SDS-PAGE, Western Blot, ELISA, PCR and qPCR, DNA extraction, RNA isolation, cDNA synthesis, cell culture, IHC staining, HPLC, cGE, cIEF, Affinity Chromatography, SEC, Ion exchange chromatography

• Technical: Microsoft Office Suite, PERL, Ras Mol, SPDB Viewer, BLAST, FASTA, Chem Draw, Marwin Sketch

EXPERIENCE

NYU Langone Medical Centre Research Volunteer New York Nov’16-May’17

• Performed DNA extraction from SOX2 knockout mouse tails using the digestion procedure and determined the genotypic characteristics using PCR and Gel electrophoresis

• Frequently Passaged, maintained and Cryopreserved 202 Cas9 cell lines for future experiments

• Transferred CRISPR Cas9 clones to 10cm plates and further sub cultured the cell lines for the protein extraction and Western Blot Analysis

Bangalore Biotech Labs Research Intern Bangalore, India Nov’14- May’15

• Performed Sub-culturing, Cryopreservation and MTT Assay on 96 well plates using BHK-21 mammalian cell lines to preserve and determine the viability of the cells for further experiments

• Maintained accurate records of experiments and analysis for a given project, conducted literature searches, and kept an up-to-date inventory for the lab equipment, buffers and the stock solutions

• Purified BSA using various chromatography techniques as a part of the quality assurance tests

LUPIN Pharmaceuticals Research Intern Pune, India Jan’14- May’14

• Detected the presence of HIV antigen by performing ELISA using Human serum IgG primary antibody and HRP linked secondary Anti IgG antibody

• Designed laboratory experiments to confirm the feasibility of processes and electrophoretic techniques such as SDS-PAGE, Iso electric focusing, Capillary gel electrophoresis, Capillary Iso electrofocusing and RP-HPLC used in the characterization of monoclonal antibodies with different characteristics

GCC Biotech Project Trainee Kolkata, India July’13-Aug’13

• Extracted DNA from the Rice leave samples obtained from different states using CTAB method to detect the presence of any mutation as a part of the quality assurance tests

• Executed PCR and Gel electrophoresis using the Rice DNA samples to establish the genotypic characteristics

LEADERSHIP EXPERIENCE

NYU Tandon School of Engineering Graduate Orientation Leader New York Jan’17

• Served as a liaison between graduate students and the office of Graduate Student Activities & Resource Center, NYU

• Guided the incoming graduate students in academic and professional sectors

• Reached out to sponsors for budgeting of various events during Spring’17 Orientation by exhibiting exemplary sponsor imploration skills

NYU Tandon School of Engineering Market Research Intern New York Jan’16-Feb’16

• Conducted research on various universities to monitor the top-tier Undergraduate and Graduate courses

• Catalogued departmental assessments based on peer reviews to revise the Faculty profile on university’s database using Microsoft Excel and Power Point

• Provided comprehensive report on New York University rankings from 1831-2016 and projected the growth rate using Tableau NYU Chemical & Biomolecular Engineering Department

Teaching Assistant New York Sep’15-Present

• Mentored a class of 100 students in Biological Sciences and increased their interest in higher learning

• Assisted and familiarized the students about the sterile techniques and good laboratory practices while performing the experiments

ACADEMIC PROJECTS

• Biotechnology and Pharmaceutical Industry – Drug to inhibit hunger signals and fight obesity

• Performed extensive literature review and hypothetically designed a theoretical novel drug Obestop to inhibit the feeding signals from the hypothalamus and prevent excessive dietary intake

• Stressed upon the clinical trial designs demonstrating the success of the novel drug over the Placebo.

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