Charlene A. SHAW, RLATg, RVT

*** ***** **

Centreville, Md. 21617

410-***-****

Objective: To obtain a challenging career, while utilizing my education and professional experience with a growth oriented company involved in animal welfare.

Experience: WIACAR, LLC

9/28/11 to Present

Oversee all operations, projects, and Facility Quality Control. Responsible for ordering, budget distributions, limits and reconciliations, Time Management Coordinator for all projects, Regulate SOP’s, Protocols, MSDS, and Hazards. Accounts payable/receivable, scheduling employees, training, Insure facility compliance with federal regulations, submit weekly and monthly reports to Director and Review all technical proposals for approval. Supervise and Direct employees daily activities, Performance evaluations, new hires, HR compliance, payroll and training. Order all equipment for facility and ensure certifications. Some travel to inspect new equipment needs and representative for company at all conferences, meetings, and Inspections. Draft and finalize all companies contracts, company documentations, submissions, and reports.

Priority One Services Bethesda, MD

Project Manager 4-6-09 to 9-26-11

Supervise the NIDCR (Dental and Cranial Facial Research) Research Facility. Responsible for all facilities research transgenic and congenic strains of mice and consists of eight animal rooms, three procedure rooms and cage wash. Supervising includes fifteen people: 2 Assistants, 4 Veterinary Techs, 6 Caretakers and 3 Cage Wash Techs. My duties include insuring that the animal care staff carries out their daily responsibilities with the highest degree of quality possible in order to comply with AAALAC and NIH standards. Other responsibilities include implementing Disaster Plan, reporting any environmental and mechanical deficiencies that occur throughout the facility, preparing daily and monthly reports for submission to the government veterinarian and program coordinator, making weekend and holiday schedules, preparing SOP’s, training staff on all research projects, SOP training, Certification training, Contract reviews, Government Liaison between Contractual personnel. Protocol review and approvals for Animal Research compliance, Time Management Coordinator for all Research studies, compiling property lists, budget reconciliations, maintaining inventories and submitting weekly census reports. Responsible for all Pathology submissions & necropsy reports, perform monthly quality assurance inspections, and Quality Control, ordering supplies, interviewing and hiring new employees, HR compliance, documenting deficiencies in the performance of staff members and performing terminations when necessary.

Priority One Services Bethesda, MD

sistant Project Manager/Trainer 6-9-08 to 4-5-09

Responsible for all daily aspects of AAALAC accredited facility, including; daily operations, training, power point presentations for National Institute of Allergies and Infectious Disease, maintain compliance of facility SOP’s, Supervise Staff and oversee compliance regulations and issues,. and new protocol training, scheduling and facility maintenance. Review annual SOP’s and write SOP’s as needed, review protocols for ACUC, supervise Staff, schedule procedure’s and technical requests, Coordinate Government PI’s studies and oversee work and quality, weekly and monthly census reports, and access software applications. Daily facility reports to Project Manager, Monthly inspections, submit and review diagnostic submissions and results. Coordinate space allocations, budget management and ordering of government supplies (given credientuals)

Scientific Applications Inc. Bethesda, MD

of Technical Operations (Large Animal) 6-7-06 to 6-5-08

Responsible for all aspects of AAALAC accredited facility of large animals. In charge of daily facility operations including: supervision of staff, liaison between contractual personnel and government staffing, ordering, facility maintenance, scheduling staff, lead surgical technician, inventory, budget and bank reconciliations. Closely monitor surgical procedures and clinical animal health issues as well as behavioral enrichment and social pairing. Keep accurate records on ordering for yearly budget requests, per diems, SOP’s (Standard Operational Procedures) and operational expenses. Maintain controlled drug logs and inventory. Training compliance for staff including SOP training, technical, new hire, and procedural. Responsible for Reports to FDA, review, approval and compliance of all protocols, animal record keeping, facility maintenance, GLP studies, training of employees and payroll.

Microsoft Word, Excel, Power Point, Outlook, Filemaker Pro, HP photosmart, Delpro ordering system, Image ready, Adobe, SSH secures Shell, NBS system, and Oracle.

Molecular Imaging Program (MIP)

Research Associate 1/05 to 6/7/06

In charge of all animal research studies for Molecular Imaging Program and Veterinary Intern Program. Provide assistance and guidance to PI’s working on animal protocols including; Anesthetics and Post-operative care. Conduct all Imaging studies, including Magnetic Resonance Imaging (MRI), Pet, Maestro (fluorescent imaging), Ultrasound, and Fibered Confocal Fluorescent Molecular Microscopy (Cell Vizio). Conduct Bio distributions, Pharmacokinetic and Therapeutic studies for Radiation Oncology Branch. Tissue Preparations for Pathology review, Authorized radiation/irradiation user through radiation Safety Branch. In charge of maintaining laboratory in compliance with Safety, AAALAC, and ACUC regulations. Provide guidance to PI’s for Protocol amendments, renewals, policies, and government regulations. Train Post docs in animal technical procedures.

LASP Program

Sentinel Program Coordinator- 3/04 to 1/05

Coordinated all Sentinel testing, ordering, environmental testing, and all Imports & Exports (international and Interstate) for five NCI Animal Facilities/Laboratory Programs. Maintained all Sentinel Animal Health records. Handled all quarantine procedures, rederivations of infected animals, and support for PI’s that need technical guidance.

Sr. Research Associate – 4/02 to 3/04

Comparative Molecular Pathology Unit/NCI

In-Vivo studies involving Magnetic Resonance Imaging, Computed Tomography, Ultrasound (C256), Xenogen, Proteomics, and pharmakinetics, using predisposed rat and mouse models for uterine, prostate, breast, and colon cancer studies non-invasively. Contrast agents used Gadolinium (Magnavest @), Oxilan, Barium Sulfate, and other suitable agents. Surgical procedures involve necropsy for immunohistochemistry, tissue preservation, surgical anesthesia, preanestetics, BP measurements, IV tail injections, gavage, colon and urethra Catherization, intubation, retro-orbital bleeds. Demonstrated talent in performing managerial and supervisory duties. Excellent verbal and written communicator exhibiting a thorough knowledge of regulatory and ethical standards and the ability to apply and enforce appropriate practices. Energetic, compassionate volunteer possessing a proven understanding and concern for current issues and challenges facing diverse human and animal populations. Responsibilities also include training of Research staff on technical and surgical procedures of research animals, instructing investigators on step by step processes needed for new protocols, and amendments for admittance to MIF facility.

Filemaker Pro, HP photosmart, Image ready, Adobe photoshop, and SSH secures Shell. Data transfer from imaging modalities for comparison studies, using SSH secure transfer client, Efilm, MRIcro, Image J, and digital jacket.

Priority One (NIAID, NIH) Rockville, MD

Facility Manger - Twinbrook II 3/01 to 4/02

animal caretakers, assistant supervisor, technicians, and cage wash personnel to support research in an AAALAC accredited facility. Maintain Sentinel programs for quarantine and general population colonies. Experience with Lagomorph’s, Mus Musculus, and Non- Human Primates. Handled all imports and exported animals. Coordinated timed mating’s of mice, inoculations, phlebotomy and tissue harvesting schedules. Provided direct and indirect research support and training for investigators and technicians. Performed daily health observations, veterinary treatments, and routine and quarantine examinations of research animals. Maintained veterinary records in accordance with AAALAC guidelines. Provided research support (equipment, personnel, etc.) for NIAID researchers. Coordinated facility repairs and improvements. Established facility schedules for routine and periodic cleaning and maintenance as well as weekend husbandry. Responsible for training of staff in SOP’s (Designing and Implementing new SOP’s ), AALAS (voluntarily for interested employees in studying for ALAT, LAT, LATG) new hires, Safety procedures, and Contracted procedures and guidelines.

Computer Programs: Delpro, Microsoft Word, Excel, Access, Power Point, Outlook and Crystal Reports

Priority One (NIAID, NIH) Rockville, MD

Lab Animal Technician – Twinbrook II 6/00 to 3/01

Supervised and provided continuing education to level II & III animal caretakers (ALAT and LAT). Coordinated timed mating’s, inoculations, phlebotomy and tissue harvesting schedules. Performed: inoculations via oral gavage, subcutaneous, intraperitoneal, tail vein and footpad injections; phlebotomy (tail vein, retro-orbital sinus and cardiac puncture); sterile tissue harvesting for ongoing research (bone marrow, spleen, lymph nodes, etc.); identification of research animals with ear tags and ear punches, tissue collection for DNA analysis of tail and ear samples, irradiation of animals for research projects using a Gamma irradiator, collection and cryopreservation of sperm for banking and in vitro fertilization, collection of tissues and various samples for histology and diagnostic evaluation. Assist in survival surgical procedures involving mice and nonhuman primates. Provided direct and indirect research support and training for investigators and technicians. Performed daily health observations, veterinary treatments, and routine and quarantine examinations of research animals. Maintained veterinary records in accordance with AAALAC guidelines.

Demonstrated knowledge of NIAID Animal Study proposals, Standard Operating Procedures, and procedures required by the NIH Division of Safety for the safe operation of an Animal Biosafety Level 3 Suite.

Computer Programs: Microsoft Word, Excel, Access, Power Point, Outlook and Crystal Reports

Priority One (NIAID, NIH) Rockville, MD

Lab Animal Caretaker – Twinbrook II 3/00 to 6/00

Provided daily husbandry and routine observations to laboratory animals under approved research studies. Maintained breeding records to include pedigree, production and weaning records. Maintained accurate animal inventories of animals in production and on study for NIAID researchers.

Arnold Pet Station Arnold, MD

Veterinary Technician 9/98 to 3/00

Assisted with the provision of routine and emergency veterinary medical and surgical care. Duties included: veterinary treatments of hospitalized animals as directed by the veterinarian, administration of injections, performance of radiography for diagnostic and OFA evaluation, clinical laboratory procedures (fecal flotation examinations, complete blood cell counts, serum chemistries, heartworm testing, ectoparasite exams and diagnostic cytology); maintenance of surgical instruments, preparation of sterile packs and assisting with surgeries; restraint of animals for examination, sample collection and induction of anesthesia; dental scaling and polishing; phlebotomy of companion animals, exotics, and client education. I was also responsible for data entry of medical procedures, medications, inventory management, and client billing.

Bench Experience:

Preparation and Conduction of In-Vivo and In-Vitro specimens for Fluorescent Imaging using Cell Vizio, and Maestro (Bio-Illuminescence). Histo-pathology tissue collection, preparation (paraffin blocking) and analysis, Conduct Bio-distribution studies and process samples, and collect data in spread sheet. Tissue collection, preservations and prep for Histopathology, necropsy and cryopreservation. Blood chemistries and analysis, Cell Culture, Cell Counts, Urine collection, Ph/Sediment, Electrolyte analysis, Microscopic Blood Cell Counts, Run PCR testing, Tissue collection for Western blot and Preparation, Irradiation of study mice, dosing, radioactive material (ex: Indium 111) Preparations, Injections, and tissue collection through half-life to end point and bio-assay collection and analysis for toxicology studies.

Vetsmart Annapolis, MD

Lead Service Coordinator, Vet tech 6/94 to 9/98

Multi phone lines, customer service, client recovery,

Payroll, scheduling, treatments, X-ray, Sx prep/assistant, cytology, phlebotomy.

Pulaski Veterinarian Hospital Baltimore, MD

Vet. Tech 1985 - 1993

Radiology, clinical treatments, surgery assistant, clinical laboratory procedures, including but not limited to, CBC’s, heartworm testing, chemistries, endoparasitic exams, phlebotomy, etc…

Belair Road Animal Hospital Baltimore, MD

Vet Tech/Assistant Tech 1983 – 1985

Clinical treatments, surgery assistant, radiology, EEG’s, laboratory procedures, Serum chemistries, CBC’s, fecal flotations, restraint, etc….

Education:

Veterinary Sciences

Essex Community College

Essex, Maryland. 21237

Biology, Veterinary Sciences

Dundalk Community College

5 to 9/97

7 to 9/99

Certification:

Certified Veterinary Technician

Certified Laboratory Animal Technician

American Association of Laboratory Animal Science

Registered laboratory Animal Technologist

– International Laboratory Animal Management

ILAM (2yr Management Program)-Memphis, TN

Professional Affiliations:

American Association of Laboratory Animal Science (AALAS)

Present

Laboratory Animal Management Association (LAMA)

2007-Present

References:

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