Assistant Quality Control
Resume:
Oak Lawn, IL ***** Phone: 708-***-****
Email:*************@*****.***
Education:
University of Illinois at Chicago (UIC), Chicago, Illinois
Bachelors of Science: August 2007
Major: Biological Sciences
Laboratory safety: I have held general laboratory safety, bloodborne
pathogen safety, and radiation safety certificates from the University of
Illinois at Chicago. Additionally I have completed both MRI safety and
controlled substance training at Northwestern University.
EXPERIENCE:
Lab Tools: Thermo-Finnigan Delta plus-
XL mass spectrometer
Stratagene Mx3000 RT-PCR machine
Corbett Research RT-PCR machine
ABI 7500 RT-PCR Machine
Lab Skills:
Luciferase Assay
DNA, RNA, Protein Extraction
Gel electrophoresis of DNA, RNA, and proteins
Silver Staining
Iron Staining
May Grunwald Staining
Tissue culturing
Immuno Staining
MTT cell proliferation assay
Western blot
Cell cycle analysis using flow cytometry
PCR (Standard, Real Time, Reverse Transcription, and
Multiplex varieties)
Elisa
Utilize Costech Elemental analyzer
Computer Skills: Microsoft Office (Excel, PowerPoint, Word)
Graph Pad Prism
Novopath
Internet Programming: HTML
Employment:
Hematogenix Laboratory Services
Laboratory Technician April 2010-
December 2011
. Utilizing standard and RT-PCR to diagnose mutations related to
cancer.
. Extracting DNA and RNA from patient blood, bone marrow, and tissue
samples.
. Preparing smears of peripheral blood and bone marrow for
morphological staining
. Preparing peripheral blood, bone marrow, and tissue samples for
analysis via flow cytometry.
. Entering patient test result data into Novopath software.
. Utilizing quality control and maintenance protocols to ensure
accurate and reliable test results.
. Ensuring departmental compliance with College of American
Pathologists (CAP) standards.
. Training new technicians and supervising their work.
. Assisted in the development of quantitative BCR/ABL testing for the
monitoring of
CML.
Umi and Abi Medical Services
Research Assistant February 2009-
Present
. Maintaining database of patients involved in ongoing clinical
research studies and entering pertinent information from medical
records into the database.
Department of Physical Therapy at Northwestern University, Chicago,
Illinois
Research Associate April 2009 -
June 2009
. Assisting in animal studies to determine effects of spinal injury
on muscle tissue.
. Attending weekly wound clinics to recruit patients for ongoing
research protocols.
. Utilizing Matlab software to analyze data from animal studies.
Department of Digestive Disease and Nutrition at UIC Hospital, Chicago,
Illinois
Visiting research specialist June 2007-
January 2009
Transfection of cells to study the activity of NHE2 and NHE3 genes.
Utilizing gel shift techniques to study binding interactions between
proteins and nucleic acids.
. Utilized RT-PCR as a means of studying genetic regulation of NHE
genes.
. Studied the effects of Enteropathogenic E-coli (EPEC) on NHE3
expression in C2BBE1 cells.
. Managed ordering of laboratory supplies and maintained records of
purchases for accounting purposes
Department of Anesthesiology at UIC Hospital, Chicago, Illinois May 2006
- May 2007
Laboratory research assistant
. Preparing tissue samples for both western blotting and PCR analysis.
. Utilizing western blot and immunochemical techniques to detect various
proteins both intra and extra cellularly.
. Reverse transcribing RNA to obtain DNA for RT-PCR analysis.
. Using agarose gel electrophoresis to verify the results of PCR
reactions.
. Analyzing the results of the above techniques and assisting in
devising new methods to increase the accuracy and reproducibility of
various laboratory results.
Department of Immunology at Rush University, Chicago, Illinois October 2005
- May 2006
Laboratory assistant
. Assisted lab personnel with developing Elisa protocol to detect both
Interferon Beta and Interleukine-8.
. Assisted in the extraction of DNA from tissue samples for use in
various PCR reactions.
. Assisted lab supervisors with studies to determine the effect of TLR
ligands on the rate of infection of Cytomegalo Virus in human cervical
tissue.
. Graphed data and discussed results and their ramifications with
supervisors.
. Maintained lab inventory and ordered supplies when needed.
. Maintained sanitary laboratory conditions by disinfecting
workstations.
Department of Surgical Oncology at UIC Hospital, Chicago, Illinois May
2005 - August 2005
Laboratory Assistant II
. Culturing and preserving various lines of cancer cells extracted from
humans.
. Studying the effects of several experimental cancer treatments on tumor
cells grown in mice.
. Using the MTT assay to determine efficiency of experimental treatments on
cancer cells.
. Using immuneohistochemistry techniques to stain cells for Ki-67 and
apoptosis.
. Treating cancer cells for cell cycle analysis using a flow cytometer
. Using Western blot technique to test for the presence of various proteins
Department of Biological Science at UIC, Chicago, Illinois
Senior Laboratory Assistant February 2004 to May 2005
. Assist with research involving stable Carbon isotopes
. Prepare soil and root samples for elemental analysis
. Analyze Carbon Dioxide samples for their Carbon isotope content
. Preparing and analyzing soil samples for isotopes and Nitrogen.
. Documenting sources for pending journal articles
. Train new lab aides and supervise their experiments
. Collecting Carbon Dioxide and plant samples in the field
. Growing plants for experimentation
ABSTRACTS & PUBLICATIONS:
Vujicic S., White L.R., Aasly J.O., Othman A., Petry K.G. and Boullerne
A.I. Intrathecal IgM to S-nitrosocysteine correlate with multiple sclerosis
relapses, under review at Journal of Neuroimmunology.
Othman, Ahmad, Firm, David M, Vujicic, Snezana et at. (2011) Olig1 is
expressed in human oligodendrocytes during myelin regeneration. Glia 59 914-
926
MD Ruhul, Amin, Ghannad, Leda, Othman, Ahmad et al. (2009) Transcriptional
regulation of the human Na+/H+ exchanger NHE3 by serotonin in intestinal
epithelial cells. Biochemical and Biophysical Research Communications, 382,
620-625
Altered Promoter DNA Methylation Regulates the human NHE2 Gene
Transcription
Eleanor J. Murray, Ying X Zhu, Ahmad Othman, Pradeep K. Dudeja,
Krishnamurthy Ramaswamy, Jaleh Malakooti
Submitted to Digestive Disease Week San Diego 2008
Intrathecal IgM Response Against S-nitrocysteine in MS Patients
Vujicic S, Othman A, and Boullerne AI.
Department of Anesthesiology, University of Illinois at Chicago
Submitted to Society for Neuroscience Chicago Chapter 2008 Annual Meeting,
also accepted for presentation at the 2008 World Congress or Treatment and
Research in Multiple Sclerosis Conference Montreal Canada. Accepted for
publication in Journal Multiple Sclerosis: Clinical and Laboratory Research
Expression of Nitric Oxide Synthase isoforms in primary cultures of Human
Oligodendrocytes.
Ahmad Othman*, Snezana Vujicic*, and Anne I. Boullerne*
Department of Anesthesiology, University of Illinois at Chicago
Presented at The UIC College of Medicine Research Day 2008
Expression of Transcription Factors and Growth Factor Receptors in Human
Oligodenrocytes
Othman, A, Firm, D.M., Aranson, B.G., Vujicic, S, Boullerne, A.I.
Submitted to ISN and ASSN 21st Biennial Meeting Cancun Mexico 2007
A PPARdelta agonist promotes differentiation of oligodendrocytes from
oligospheres
A.I. Boullerne*, P. Polak, S. Vujicic, A. Othman and D.L. Feinstein
University of Illinois at Chicago, Chicago, Illinois, USA.
Submitted to the 40th annual Conference on Brain Research at Snowmass
Colorado.
My work in researching stable isotopes has been acknowledged in an article
published in the August 2005 edition of Global Change Biology. The
citation is listed below.
Trueman Rebecca, Gonzalez-Meler Miquel (2005) Accelerated belowground C
cycling in a managed agriforest ecosystem exposed to elevated carbon
dioxide concentrations. Global Change Biology, 11, 1258-1365
I am also in the process of analyzing data for several possible journal
articles I will be coauthoring with research supervisors.
Contact this candidate