GANGA NARASIMHAN

**** ********* ****, *** # ***, Arlington, VA - 22209

abz4fh@r.postjobfree.com, abz4fh@r.postjobfree.com 202-***-****[pic]

EDUCATION

Georgetown University

Washington, DC

MS, Biochemistry and Molecular Biology

May 2013

. GPA - 3.63

Bangalore University

Bangalore, India

MS, Biochemistry

June 2010

. GPA - 3.60

Bangalore University Bangalore,

India

BS, Biotechnology, Botany, Chemistry

July 2008

. GPA - 3.95

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CERTIFICATIONS

Radiation and Laboratory Safety Training,

Initial Blood borne Pathogen Training

Good Laboratory Practices (GLP),

Good Manufacturing Practices(cGMP) [pic]

EXPERIENCE

Research Intern at Georgetown Lombardi Comprehensive Cancer Center

Washington, DC

Dec 2012

- Present

Development of method for the detection and quantification of site and DNA

adduct specific mutation in Human Cells.

- Plasmid Construction, DNA Cloning, Transformation (Heat Shock and

Electroporartion)

- Restriction Digestion, Plasmid Isolation using Mini Prep and Maxi

Prep, Site directed mutagenesis.

- Preparation of Single Stranded DNA using helper phage and Qiagen M13

Kit, Preparation of Double stranded DNA from ssDNA.

- Transfection(Lipofectamine), Cell splitting.

- PCR, RT-PCR, Touch down PCR

Slow repair of Etheno-adenine at mutation hotspots in p53

- Plasmid Construction

- Transfection, Cell splitting, Transformation by Electroporation into

XL1 Blue cells.

- Plasmid isolation

- Sequencing using bio-informatics tools like multiple sequence

alignment (Bioedit), NCBI-BLAST.

Purification of human MPG protein from E. coli using FPLC

- Transformed pRSETb-hMPG expression vector into Ecoli system for the

expression.

- Purified recombinant DNA repair enzyme Human MPG in the E.Coli

expression system through ion-exchange chromatography with AKTA

FPLC system

- Identified the purified protein by SDS PAGE.

Teaching Assistant at Georgetown University Department of Biochemistry

Washington, DC

Jan 2013 - May 2013

Preparing Biotechnology Lab for the graduate students and assisting in

performing the experiments.

- Reagent Preparation, Reagents cataloging, Aliquoting the reagents, Lab

maintenance.

Research Intern at Georgetown University Department of Biochemistry

Washington, DC

Sep 2012 - Dec

2012

Melanoma cancer initiating stem cells.

- Growing Phoenix, fibroblast cell line

- Cloning and Transfection

- Cell Splitting, Cryopreservation

- Restriction Digestion, Plasmid Isolation using Mini Prep and Maxi Prep

kit.

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TRAINING

. Add on course on plant biotechnology Specialized in plant tissue

culture.

. Participated in two day workshop on RAPD & Blotting Techniques at

KLE's society S. Nijilingappa College, Bangalore, India organized by

M/s Wingene Biotech Research Labs,

. Participated in one day workshop on Automation in Life Sciences at M.S

Ramaiah College of Arts, Science and Commerce, Bangalore, organized by

PROBIOSYS, Bangalore India

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SKILLS AND EXPERTISE

Molecular Biology Techniques

. Isolation of Plasmid by Maxi Prep Method, Mini Prep Method, Site

directed Mutagenesis, Single Strand DNA Isolation, Transformation by

Heat shock and Electroporation, DNA Isolation, RNA isolation, Plasmid

Construct Preparation, Isolation of single- stranded DNA, Preparation

of dsDNA from ssDNA, Ligation, Preparation of bacteriophage

suspension, Restriction digestion (EcoR1, Sma 1, Dpn 1), PCR, RT-PCR,

Touch Down PCR, DNA isolation from the Gel, FACS, ELISA, SDS-PAGE,

Western Blotting, Spectrophotometer, Colorimeter, UV

spectrophotometer, Speed Vac.

Cell Culture Techniques

. Transfection, Primary Explant Culture from liver, kidney & pancreas,

Cell Isolation, Sub culturing of cells, Cryopreservation, Maintaining

mammalian cell line/Stem Cell line, splitting of Cells,

Heamocytometer, Hoechst staining, Annexin V FITC/PI Staining,

Flurometric assay of Caspase-3 Activity, Giemsa Staining, Cell cycle

analysis, Dilution Cloning, Colony Isolation, Apoptosis analysis,

Chromosomal Spread for karyotype analysis,

. 3T3 Fibroblast cell line(Mouse), MDA -MB-231 Epithelial cell line,

Jurkat T.Lymphocyte, SH-SY5Y Neuroblastoma, HepG2, HEK293, CD133

cell.

Microbiology techniques

. Plating techniques, Isolation, purification and maintenance of

bacteria, Bacterial growth curves, Induction and isolation of mutants

in bacteria, preparation of competent cells in bacteria, Enumeration

of bacterial numbers by serial dilution and plating, Determination of

Viable count of bacteria, Ouchterlony double diffusion.

Chromatography techniques

. Circular paper Chromatography, Thin Layer Chromatography, Column

Chromatography (CM-Sephadex Cation Exchange Column, Cobalt Agarose

Column), HPLC.

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PROJECTS

Fermentation, Georgetown University

Feb 13 - Apr 13

Shake flask fermentation in E. coli, Bench top fermentation, purification

and characterization of Lysozyme from egg by Ion Exchange Chromatography

and ?- Galactosidase by Affinity Chromatography, Ultra & microfiltration.

- Identified the purified protein by western blotting and SDS PAGE.

- Characterization of proteins by Bradford Coomassie Blue protein

assay, Lysozyme activity assay and ?- Galactosidase activity

assay.

HPLC, IAH & VB Hebbal, Bangalore India

Standardization of Enrofloxacin in HPLC for the analysis of amount of

enrofloxacin residue in chicken sample.

Pro Nano Technology, Inc

Jan 12 - May 12

Involved in setting up an imaginary company called Pronano Technology, Inc

Also associated with writing an SBIR Grant, SBA application, Side by side

revenue tactics, developing a novel drug/product, designing an efficient

yet novel mechanism for the drug action, assessing its balance sheet,

income statement, commercializing the product into the market

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SIGNIFICANT POSTER/ORAL PRESENTATION

"Development of method for the detection and quantification of site and DNA

Adduct specific Mutation in human cells"

Ganga Narasimhan1, Jordan Woodrick2, Suhani Gupta2, Anand Chetram2,

Rabindra Roy2

"Regenerative Medicine: Stem Cell and Future"

. Presented a seminar on Regenerative Medicine: Stem Cells & Future on

Manipal Institute of Regenerative Medicine, Bangalore, India.

"Human genome project"

. Presented a seminar on Human genome project in one day state level

seminar for students conducted by Science Forum KLE's society S.

Nijilingappa college, India.

"Cloning"

. Presented a seminar on Cloning in one day state level seminar for

students conducted by Science Forum KLE's society S. Nijilingappa

college, India.

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REFERENCE

. Dr. Cynthia M. Simbulan-Rosenthal (email: abz4fh@r.postjobfree.com,

phone: 202-***-****)

Associate Professor, Director - MS Biochemistry & Molecular Biology

Program

Rm : 319 Basic Science, Department of biochemistry and Molecular &

cellular Biology

Georgetown University School of Medicine

3970 Reservoir Rd. NW, Washington, DC 20057

. Dr. Rabindra Roy (email: abz4fh@r.postjobfree.com, phone: 202-***-****)

Associate Professor of Oncology,

S122 (Lombardi Comprehensive Cancer Center)

3800 Reservoir Rd. NW, Washington, DC 20057

. Jordan Woodrick (email: abz4fh@r.postjobfree.com phone: (202)-687-

3509, 601-***-****)

Tumor Biology Doctoral Student at Georgetown University

S120 (Lombardi Comprehensive Cancer Center)

3800 Reservoir Rd. NW, Washington, DC 20057

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