Pranali Taskar Resume

**** ******** **, *** ****, San Diego, CA, 92122

Cell: 213-***-****

Academics:

****- **** :

Masters of Science (MS) in Molecular Microbiology and Immunology from University

of Southern California, GPA 3.5/4.00

Relevant Coursework:

Cell biology, Pathology, Biochemistry, Molecular biology of Cancer, Infection and host

immunological response, Biostatistics, Structure and management of clinical trials

Certified in AALAS animal training certificate course

2007 – 2011:

Bachelor of Technology (B.Tech) – Biotechnology from Dr. D. Y. Patil University –

Dept. of Biotechnology & Bioinformatics, Aggregate: 65.28% (Sem I – VIII).

Relevant Coursework

Immunology, Microbiology, Virology, Industry Management, Genetics, Animal Tissue

Culture, Plant Tissue Culture, Bioinformatics, Statistics.

Research:

Tranalational and post-translational regulation of Hemeoxygenease -1 in response to

PlGF.

Towards MS Thesis: (September 2012 to May 2013)

Placental Growth Factor mediated transcriptional and post-transcriptional regulation of

Hemeoxygenase-1. PlGF levels are elevated in patients with sickle cell disease.

My research involved studying the response of the endothelial cells to PlGF, from initiation of

cell signalling till post translational effect on the HO-1 mRNA.

Isolation and Genetic characterization of Hepatic tumor initiating stem cells.

Previous graduate research (January 2012 to August 2012)

Characterization of Human and Mouse hepatic Tumor Initiating stem cells freshly isolated

from mice. Colony formation assays, methylation studies and karyotyping performed.

Maintained stable transformed cell lines in BSL 2+ facility.

Cytogenetic analysis of Acute Myeloid Leukemia

(January 2011 to June 2011)

Under geneticist Dr. Bani Bandana Ganguly,MGM Hospital, Vashi, Navi Mumbai, India.

The project involved culturing of cells from blood and bone marrow samples of patients and

examining them for cytological abnormalities and profile their karyograms.

2

Skills involved:

microRNA isolation Plasmid construction and purification

Gene promoter and 3’UTR sequence construct synthesis

qRT-PCR for miRNA and

mRNA

Bacterial transformation RNA extraction

Human cell transfection Protein extraction and western blot analysis

Cell culture (Primary. Karyotyping, genotyping

Transformed, Hematopoetic,

Stem cells and cancer cell

lines.)

Animal (Mice)Handling,

Publications: European Journal of Human Genetics, May 2011

1. Genetic Basis of Bombay blood group

2. Chromosomal abnormalities in follicular thyroid carcinoma

3. Genetic basis of Rhabdomyosarcoma

Technical Training:-

Trained in the Microbiology department of the Pathology section of Jupiter Hospital,

Thane.

Trained in department of histopathology and microbiology at MM medical center and

diagnostics, Mumbai,

Attended training conducted by Onan Biotech Pvt. Ltd. in the college, givin g hands- on

training in various molecular biology practical techniques like RT -PCR, RFLP, IEF,

Mononuclear cell culture etc.

Computer Skills:-

Software: Bioedit, Rasmol, Swiss pdb, Ligand Scout, Modeller, Chemsketch,

Marvinsketch, DS Visualizer, Argus Lab etc.

Operating Systems: W indows, Mac

Application: MS office, MS excel

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