Molecular Biology,Molecular Tox,real-time PCR,cell culture,ELISA, FACS

TULIKA TYAGI http://www.linkedin.com/pub/tulika-

tyagi/18/586/885

**** **** *****, *** *** ************@*****.***

San Mateo, CA, 94403 980-***-****

OBJECTIVE:

To obtain a Research Associate/Associate Scientist position in a biotechnology or

pharmaceutical industry where I can effectively utilize my skills in molecular biology,

epigenetics and toxicology

EDUCATIONAL QUALIFICATIONS:

Master of Science, Environmental Toxicology January 2011 – May 2013

Texas Tech University, Lubbock, Texas (GPA: 4.0)

Bachelor of Technology, Biotechnology June 2006 – August 2010

Vellore Institute of Technology, Vellore, India

SKILLS:

• Real-time PCR, flow cytometry, primer design

• Western Blot, ELISA, SDS-PAGE, HPLC

• DNA/RNA isolation, protein isolation

• Mammalian and bacterial cell culture

• Cell based assays (viability, apoptosis, proliferation, tumorigenicity)

• Microarrays, Pyrosequencing, RAPD

• Cell transfection, gene cloning, si/shRNA knockdown

• Mice Handling (IP, SC injections)

COMPUTER SKILLS:

• Microsoft Office, ImageJ software, GraphPad Prism

WORK EXPERIENCE:

• Research Assistant, Molecular Toxicology and Carcinogenesis - Texas Tech

University (Sept 2011-May 2013)

• Performed real-time PCR/RT-PCR to evaluate gene expression changes in cancer

cells

• Performed flow cytometric analysis to study effect of drugs on cancer cell cycle

and survival

• Performed Western blot analysis to study changes in expression of proteins

involved in cell survival, growth, DNA methylation and histone modifications

• Performed pyrosequencing and methylation sensitive RAPD PCR to study gene-

specific and global CpG methylation patterns in cancer

• Performed IC50 assays to evaluate cytotoxicity of natural compounds and

chemotherapeutics

• Performed soft agar colony growth assay to study tumorigenicity of cells

• Isolated protein from cells and performed quantification by Bradford method

• Isolated DNA/RNA and verified quantity/quality by nanodrop and gel

electrophoresis

• Used aseptic techniques such as laminar hood to perform cell culture maintenance

• Created tumor models and administered drugs by giving IP, SC injections to mice

• Assisted in shRNA gene knockdown, bacterial transformation and gene cloning

TULIKA TYAGI http://www.linkedin.com/pub/tulika-

tyagi/18/586/885

1075 Park Place, Apt 318 ************@*****.***

San Mateo, CA, 94403 980-***-****

• Prepared stock solution, reagents and buffers

• Intern-Indian Institute of Sciences, India ( Dec 2009 -June 2010)

• Performed gene expression profiling for breast cancer tissues by microarray

• Validated microarray results by semi-quantitative PCR

• Isolated RNA from tissue sample and performed reverse transcription

• Verified quality/quantity of RNA by gel electrophoresis and nano drop

• Optimized PCR conditions and validated normalization genes

• Assisted in collection and maintenance of tissue samples from breast cancer

patients

• Summer trainee -Biotechnology Division, Ranbaxy Labs, India (May 2008-June2008)

• Performed ELISA to detect tuberculosis antigen

• Performed total cellular protein isolation, SDS-PAGE and western blot on various

proteins

• Assisted in protein purification through HPLC

PUBLICATIONS:

• Singh KP, Treas J, Tyagi T, Gao W (2012) DNA demethylation by 5-aza-2-deoxycytidine

treatment abrogates 17 beta-estradiol-induced cell growth and restores expression of

DNA repair genes in human breast cancer cells. Cancer Lett 316(1):62-9.Epub 2011 Oct

23.

• Treas JN, Tyagi T, Singh KP (2012) Effects of Chronic Exposure to Arsenic and Estrogen

on Epigenetic Regulatory Genes Expression and Epigenetic Code in Human Prostate

Epithelial Cells. PLoS One 7(8): e43880

• Accepted for publication in The Prostate: Treas JN, Tyagi T, Singh KP. Chronic exposure

to arsenic, estrogen, and their combination causes increased cell growth and

transformation by hypermethylation-mediated silencing of MLH1 in human prostate

epithelial cells

PRESENTATIONS:

• Gave platform presentation titled "Increased growth inhibition of breast cancer cells by

combination of 5-aza 2'deoxycytidine and - epigallocatechin gallate" at T exas Tech

Annual Biological Sciences Symposium in 2013

• Presented poster on "Increased growth inhibition of breast cancer cells by co-treatment

with EGCG and 5-aza2'deoxycytidine is modulated through epigenetic mechanisms" at

annual Society of Toxicology (SOT) meeting in 2013

• Delivered platform presentation titled "Co-treatment of green tea polyphenol and 5-aza-

2-deoxycytidine increases growth inhibition of human breast cancer cells" at regional

SOT Lone Star chapter meeting in 2012

• Presented poster on "Effect of curcumin and epigallocatechin gallate in combination

with demethylating agent,5-aza-2'deoxycytidine in breast cancer cell growth inhibition"

at annual SOT meeting in 2012

TULIKA TYAGI http://www.linkedin.com/pub/tulika-

tyagi/18/586/885

1075 Park Place, Apt 318 ************@*****.***

San Mateo, CA, 94403 980-***-****

• Presented poster on "Evaluation of anti-breast cancer properties of naturally occurring

compounds" at regional SOT Gulf Coast chapter meeting in Oct 2011

AWARDS AND HONORS:

• Recipient of Waste Control Specialists 2012 Outstanding Masters Student award at

The Institute of Environmental and Human Health (TIEHH), Texas Tech University

• Recipient of Jhamandas Watumull Scholarship for the period of January-May 2012

• Recipient of the nationally competitive Pediatric Oncology Summer Training (POST)

grant by Alex’s Lemonade Stand Foundation in May 2011

• Awarded Merit Scholarship for Academic Excellence for two consecutive years (2006-

07, 2007-08) by Vellore Institute of Technology, India

ACTIVITIES & MEMBERSHIPS :

• Secretary and Treasurer of TIEHH Student Association for the academic year 2012-13

• Member - Society of Toxicology, Women in Toxicology, SOT Lone Star regional

chapter, SOT carcinogenesis specialty section

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