Management Service

Basil Perkins

Education

New York University (NYU), New York, NY

Master of Science, Biology, May 2013

GPA: 3.9

Two time recipient of the Master’s Student Research Grant – Fall 2012, Spring 2013

Master’s thesis: “Optimizing DDX6 mammalian gene knockdown”

University of California at Santa Barbara (UCSB), Santa Barbara, CA

Bachelor of Science, Pharmacology, June 2011

GPA: 3.5

Distinction in Biology

Experience

NYU, Center for Genomics and Systems Biology, Vogel Lab, New York, NY

Graduate Researcher 2012 - Present

Purified small and large scale protein and DNA extracts

Cultured adherent mammalian cells: HeLa, HEK293, NIH3T3, and HT22

Established RNA binding protein knockdown in mammalian cells by endogenous shRNA expression

Assessed the effect of transduced shRNA by western blot and MTT assays

NYU Morse Academic Program, New York, NY

Teaching Adjunct 2012 - 2013

Instructed courses: Lessons from the Biosphere – Tyler Volk, PhD; and The Brain: A User’s Guide – Efrain Azmitia, PhD

Directed weekly laboratory classes and assisted in all aspects of testing, grading, and student management

Received positive comments, and praise for patience from students on anonymized evaluations

UCSB, Marine Science Institute, Analytical Lab, Santa Barbara, CA

Research Associate 2010 - 2011

Operated isotope-ratio mass spectrometry and carbon/ hydrogen/ nitrogen element analysis and generated client reports

Responsible for maintaining reaction tubes and scrubbers in isotope-ratio mass spectrometry and carbon/ hydrogen/ nitrogen

element analysis instruments

Organized receipt of samples for analysis

UCSB, Molecular, Cellular and Developmental Biology, Hayes Lab, Santa Barbara, CA

Undergraduate Researcher 2010 - 2011

Demonstrated competence in bacterial cloning, and transformations

Conducted sucrose density gradients and fractionation of RNA-protein structures

Performed FACS analysis and competition experiments to determine binding specificity of bacterial surface proteins

Genentech, Protein Chemistry, Gonzalez Lab, South San Francisco, CA

Summer Intern 2009 and 2010

Prepared and analyzed protein micro-arrays of secreted proteins

Validated protein binding with surface plasmon resonance (SPR) on Biacore and ProteOn

Presented research at annual intern poster presentations, department meetings, and lab meetings

UCSB, Ecology, Evolution and Marine Biology, Mazer Lab, Santa Barbara, CA

Undergraduate Researcher 2007 - 2009

Researched background, designed experiment, and developed protocol for starch gel electrophoresis of plant protein

Participated in weekly lab meetings and presented current research using Excel and PowerPoint

Software skills

Microsoft Office Suite Google Docs/Drive Suite OpenOffice Suite Image J

Leadership

Young Adult Leader for Free Unitarian Universalist Network 2009 - Present

Coordinated meetings of 3-10 people to design community service events, weekend conferences for 30-50 people

Planned, facilitated and acted as registrar for district wide weekend conferences: developed and managed budget and attendee

information of 50 people

Classroom Tutor for America Reads/ America Counts 2013

Motivated East Harlem middle school students to excel in math and science

Provided individualized in-classroom learning support to groups of 1-10 6th grade students

Active member of SACNAS, UCSB Chapter 2007 - 2011

Presented current research at National SACNAS Conference

Attended weekly chapter meetings, and held a one year chair position

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