Development Health

Vijayalaxmi G Gupta, Ph.D.

**** ******* ****, *** # ***, Kansas City, KS 66103 Cell 913-***-****

E mail: ********@*****.*** www.linkedin.com/pub/viju-gupta/4/16/95b/

PROFESSIONAL OBJECTIVE

Independent and driven scientist with experience in drug discovery and development and space flight/microgravity,

looking for a challenging and stimulating environment that will help me learn more and grow as a research

professional

EDUCATION & TRAINING

Ph.D., National Institute for Research in Reproductive Health, Mumbai, India

MSc (Microbiology)., The Institute of Science, Mumbai, India

BSc (Microbiology)., Ramnarain Ruia College, Mumbai, India

Ph.D. from National Institute for Research in Reproductive Health 1999 2006

Summary: Ph.D. dissertation on, “Molecular characterization of human sperm adhesion molecules using

polyclonal/monoclonal antibody and their role in fertility”, PhD advisor: Dr. K.V.R. Reddy. I identified gene

encoding a protein that was present exclusively in sperm from fertile men and absent in sub fertile. I developed

polyclonal and monoclonal antibody against the protein with long term plan to use them as bio markers of fertility.

PROFESSIONAL EXPERIENCE

Research Assistant Professor, University of Kansas Medical Center 2011 Present

Postdoctoral Fellow, University of Kansas Medical Center 2006 2011

Summary of research work:

• Non hormonal reversible male contraceptive drug discovery and development . We use small molecule

inhibitors as lead compounds to develop a reversible male contraceptive. Currently we are in the pre

clinical trial development phase.

• Determine effect of space flight and microgravity on reproductive physiology . Worked as part of BSP (Bio

specimen Sharing Program) team, where female mice were flown on three different space shuttles (STS

131,133 and 135). We determined that 12 15 days of space flight exposure alters female reproductive

physiology. The ongoing BION M1 mission will add more info.

•

Role of protein tyrosine kinase in sperm capacitation . We determined that sperm protein kinases play a

critical role on sperm capacitation and acrosome reaction.

Duties: Design and execute research experiments, write manuscripts, present scientific data at national and

international meetings, mentor trainees and research technicians and assistants, write grants

Skills acquired:

• Formulation of compounds

• In vitro and In vivo assays

• Studies in animal models (Oral gavage, SQ, IP in mice, rats, rabbits)

• Collaborate with medicinal chemists and protein chemists: SAR (Structure activity relationship)

• Mechanism of action of compounds of interest

• Sperm analysis (routine) and motility analysis using CASA

• Sperm egg/ sperm zona binding

• Mouse In Vitro Fertilization

• Tissue histology, Immunohistochemistry and Immunofluorescence

• Spermatogenic index determination

• SDS PAGE/Western Blot,ELISA

• PCR and other molecular biology techniques

• Scientific writing

PERFORMANCE AWARDS

AND FELLOWSHIPS

• Faulty travel award – To attend SSR 2013 to be held at Montreal, Canada in July 2013

• 2011 Research Day, Second Place Award for Postdoc Research Oral Presentation

• Larry Ewing Memorial Trainee Travel award at 43rd SSR meeting, Milwaukee, Wisconsin, 2010

• Larry Ewing Memorial Trainee Travel award at 41st SSR meeting, Kailua Kona, Hawaii, 2008

• Kathleen Osborn Travel grant to attend 41st SSR meeting Kailua Kona, Hawaii, 2008

• Partial travel grant from Council for Scientific and Industrial Research (CSIR) to attend the 3rd European

Congress of Andrology, Muenster, Germany, 2004

• Best presentation award at the 3rd European Congress of Andrology, Muenster, Germany, 2004.

• Partial travel grant from European Congress of Andrology to attend the 3rd European Congress of

Andrology, Muenster, Germany, 2004. The abstract got in top 20 list

• CSIR (Council for Scientific & Industrial Research) Senior Research Fellowship (2003 –2006)

• DBT (Department of Biotechnology) fellowship from 1999 to 2001

INVITED LECTURE

Lecture delivered to Master’s (Bioscience) students on “Introduction to Research Methodology” at Kansas City

University of Medicine and Biosciences, Kansas City, MO. April 25th 2013.

SERVICE

• Training Course on Immunological Techniques and their applications, ICMR, Mumbai, 2001

• WHO sponsored Short Training Course on Dissemination of Use of Resazurin Reduction Test and Sperm

Function Tests for Infertility, ICMR, 2002

• Assisted in organizing the Forum 9, Global Forum for Health Research at Mumbai, India, 2005.

Reviewed abstracts and co chaired a session for the 43rd annual meeting of the Society for the Study of

•

Reproduction, Milwaukee, Wisconsin in July Aug 2010

Reviewed abstracts for American Society for Gravitational and Space Research meeting held at New

•

Orleans from Nov 28th –Dec 2nd 2012

Reviewed abstracts for Society for the Study of Reproduction “Reproductive Health: Nano to Global”

•

meeting to be held at Montreal, Canada in July 2013

Vijayalaxmi G Gupta, Ph.D.

3540 Rainbow Blvd, Apt # 326, Kansas City, KS 66103 Cell 913-***-****

E mail: ********@*****.*** www.linkedin.com/pub/viju-gupta/4/16/95b/

PROFESSIONAL DEVELOPMENT

• Grant writing workshops

• Clinical trials educational lecture series

• Speaking for success seminar

PROFESSIONAL AFFILIATIONS

• Indian Society for Studies in Reproduction and Fertility (ISSRF)

• Society for the Study of reproduction (SSR)

• American Society of Gravitational and Space Biology (ASGSB)

• Honorary member of Sigma Xi

ADDENDUM

Peer reviewed Publications:

• α 6β 1 Integrin is a potential clinical marker for evaluating sperm quality in men. Venkata Rami K. Reddy,

Shrimali K. Rajeev, and Vijayalaxmi Gupta. Fertility and Sterility, Vol.79.3 pg. 1590 1596 June 2003

• Identification and characterization of cDNA encoding a human sperm membrane protein sharing homology

with Huntington protein. Vijayalaxmi Gupta, Raghavan V P, Reddy K V R. J.Reprod.Imm, 66.Issue.2:

117 125, 2005

KVR Reddy, G Vijayalaxmi, K Rajeev, C Aranha. Inhibition of sperm egg binding and fertilization by

•

monoclonal antibody reactive to 57 kDa human sperm surface antigen. Reproduction, Fertility and

Development, 18(8):875 84.2006.

Joseph S. Tash, Ph.D., Lesya Holets, Ph.D., Katherine F. Roby, Ph.D., and Vijayalaxmi Gupta, Ph.D.

•

Getting More Bang for the Buck: A PI’s perspective on how the Biospecimen Sharing Program worked

successfully to maximize science return from STS 131. Feature article in Newsletter of the American

Society for Gravitational and Space Biology 26(1) Summer 2010 (pg. 15 19)

Jinping Luo, Vijayalaxmi Gupta, Brian Kern, Joseph Tash, Gladis Sanchez, Gustavo Blanco, and William

•

H. Kinsey. Role of Fyn kinase in spermatogenesis: defects characteristic of Fyn null sperm. Biology of

Reproduction. 2012 Jan 30; 86(1):1 8

Jae Chul Lee, Subhashree Francis, Dinah Dutta, Vijayalaxmi Gupta, Yan Yang, Jin Yi Zhu, Joseph S.

•

Tash, Ernst Schönbrunn, and Gunda I. Georg. Synthesis and Evaluation of Eight and Four membered

Iminosugar Analogues as Inhibitors of Testicular Ceramide specific Glucosyltransferase, Testicular b

Glucosidase 2, and other Glycosidases. Journal of Organic Chemistry. J Org Chem. 2012 Apr 6;

77(7):3082 98

Vijayalaxmi Gupta, Sudhakar Jakkaraj, Gunda I. Georg, Joseph S. Tash. N Butyldeoxygalactonojirimycin

•

Induces Reversible Infertility in Male CD Rats. Manuscript submitted to Journal of Medicinal Chemistry

V. Gupta, L. Holets, K.F. Roby & J.S. Tash. A multi investigator compatible tissue retrieval regimen of

•

male and female reproductive organs to aid in Biospecimen Sharing Program procedures for acute post

flight tissue collection and processing. Manuscript in preparation.

Vijayalaxmi Gupta, Katherine F. Roby, Michael Baltezor, Colleen Flynn, Brian Kern,Todd Hall, Sudhakar

•

Jakkaraj, Ramappa Chakrasali, Gunda I. Georg, Melinda Broward, Robyn Wood, Scott Weir, Joseph S.

Tash. KU AS 272, a potential single dose anti spermatogenic compound for cats and dogs, shows safety

and ability to block spermatogenesis in testis to Sertoli cells only in male rat. Manuscript in preparation.

Abstracts and Presentations:

Vijayalaxmi G Gupta, Ph.D.

3540 Rainbow Blvd, Apt # 326, Kansas City, KS 66103 Cell 913-***-****

E mail: ********@*****.*** www.linkedin.com/pub/viju-gupta/4/16/95b/

Vijayalaxmi Gupta, Reddy KVR. “Expression of integrin cell adhesion molecules and their ligands on

human spermatozoa with a potential role in fertilization". XVIII National Conference on Reproductive

Biology and Comparative Endocrinology, Chennai, Dec.27 29, 1999.

Rajeev KS, Vijayalaxmi Gupta and Reddy KVR. “Expression of VLA 6 integrin and ligand fibronectin

on human spermatozoa A flow cytometric analysis". XXVI National conference on Cancer Immunology

in New Millennium, CRI, Mumbai. Jan.6 8, 2000.

Vijayalaxmi Gupta, Rajeev KS, Reddy KVR. "Correlation between a 6b 1 integrin and ligand fibronectin

on human spermatozoa and their fertilization potential". International Congress on Fertilization, Embryo

development and Implantation. National Institute of Immunology, New Delhi, 6 9th Nov.2000.

Vijayalaxmi Gupta, Clara Aranha, KVR Reddy. “Molecular characterization of 57kDa Fertility Associated

Sperm Antigen identified using human spermatozoa and its role in fertility”. NCARH, held at University of

Rajasthan, Rajasthan, 5 7th February 2003.

Vijayalaxmi Gupta, Raghavan VP, Reddy KVR. “Molecular characterization of human sperm adhesion

molecules using polyclonal / monoclonal antibody and their role in fertility”. ISSRF 2004 Annual Meeting

held at Institute of Science, Bangalore, 23 25th January 2004.

Vijayalaxmi Gupta, Raghavan VP, R. Shrimali, C.Aranha and K.V.R.Reddy. Characterization of Human

Sperm Adhesion Molecules identified by Polyclonal/Monoclonal Antibodies and their Role in Fertility”.

3rd European Congress of Andrology 16th Congress of the German Society of Andrology, held at

Muenster, Germany from 11th September 14th September 2004.

Vijayalaxmi Gupta, Raghvan V.P. and Reddy K.V.R. “Characterization of cDNA encoding a human

sperm membrane protein sharing homology with Huntingtin protein”, International Symposium on

Advances and Challenges in Reproductive Health Research in the Post Genomic Era, National Institute for

Research in Reproductive Health (NIRRH), 9 12th January 2005, Mumbai

Vijayalaxmi Gupta, Raghavan V.P. and Reddy K.V.R. “Characterization of a human sperm membrane

protein sharing homology with Huntingtin protein”. International symposium on STEM CELLS : Premises

& Promises for Research & Therapeutics, National Institute for Research in Reproductive Health (NIRRH),

18 21 September 2005,Mumbai.

Vijayalaxmi Gupta, Sudhakar Jakkaraj, Gunda I. Georg, Joseph S. Tash. “Administration of candidate

male contraceptive iminosugar leads, NB DNJ and NB DGJ, by single daily oral administration in

C57BL/6 mice reveals differences in anti spermiogenic efficacy” presented at Future of Male

Contraception” meeting held at Seattle, Washington in September 2007.

Vijayalaxmi Gupta, Sudhakar Jakkaraj, Gunda I. Georg, Joseph S. Tash. “Exploiting the Potential of

Iminosugars as Novel Reversible Non Hormonal Male Contraceptive”. Presented at the 41st Society for the

Study of Reproduction meeting held in Kailua Kona, Hawaii in May 2008.

V. Gupta, L. Holets, K.F. Roby, J.S. Tash. “Definition of multi investigator compatible tissue retrieval of

male and female reproductive organs to aid in Biospecimen Sharing Program procedures for acute post

flight tissue collection”. Platform presentation at the American Society for Gravitational and Space

Biology (ASGSB) meeting held at Raleigh, North Carolina in November 2009

Vijayalaxmi Gupta, Sudhakar Jakkaraj, Gunda I. Georg, Joseph S. Tash. “In vitro and In vivo Effects of

NB DGJ and NB DNJ: Lead Compounds for Development of Non hormonal Reversible Oral Male

Contraceptives” Presented at the 43rd Society for the Study of Reproduction (SSR) meeting held in

Milwaukee, Wisconsin, July Aug 2010

V. Gupta, K.F. Roby, L. Holets, J.S. Tash. “Spaceflight and 13 days exposure to LEO has negative impact

on the cellular morphology of ovaries in C57Bl/6J mice”. Platform presentation at the American Society

for Gravitational and Space Biology (ASGSB) meeting held at Washington DC in November 2010

Vijayalaxmi Gupta, Mary Zelinski, Karen Steinmetz, Andras Miklos, Melinda Broward, Sudhakar

Jakkaraj, Ramappa Chakrasali, Gunda I. Georg, Joseph S. Tash. “Anti spermatogenic Efficacy of Non

Hormonal Male Contraceptive Agent, H2 Gamendazole, in Mice, Rats, Rabbits, and Non human Primates

(Rhesus), and a Multiple Low Dose Oral Regimen That Gives 100% Infertility with Complete

Reversibility”. Abstract accepted for platform presentation at 44th SSR meeting to be held in Portland,

Oregon July 3 Aug 3. This abstract was selected in the final round for competition.

Vijayalaxmi Gupta, Mary Zelinski, Karen Steinmetz, Andras Miklos, Melinda Broward, Sudhakar

Jakkaraj, Ramappa Chakrasali, Gunda I. Georg, Joseph S. Tash. Anti spermatogenic Efficacy of Non

Hormonal Male Contraceptive Agent, H2 Gamendazole, in Mice, Rats, Rabbits, and Non human Primates

(Rhesus), and a Multiple Low Dose Oral Regimen That Gives 100% Infertility with Complete Reversibility.

Presented at Future of Contraception Initiative meeting held at Seattle, Washington in October 2011.

V Gupta, KF Roby, L Holets, JS Tash. STS 131, STS 133 and STS 135: Spaceflight inhibits ovarian

follicle development and formation of corpus luteum in mice. Platform presentation at the American

Society for Gravitational and Space Biology (ASGSB) meeting held at San Jose, California in November

2011

Vijayalaxmi Gupta, Katherine F. Roby, Brian Kern, Todd Hall, Sudhakar Jakkaraj, Ramappa Chakrasali,

Gunda I. Georg, Melinda Broward, Robyn Wood, Scott Weir, Joseph S. Tash. KU AS 272, a Potential

Single dose Sterilant for Cats and Dogs, Shows Safety and Ability to Block Spermatogenesis in Testis to

Sertoli cells only after a Single Subcutaneous Injection in Male Rat. Presented as a talk at 45th Society for

the Study of Reproduction meeting held at State College, PA in August 2012.

V Gupta, KF Roby, L Holets, JS Tash. Spaceflight disrupts reproductive dynamics in mice ovary. Platform

presentation at the American Society for Gravitational and Space Research (ASGSR) meeting held at New

Orleans, Louisiana in Nov 28 – Dec 2, 2012

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