Assistant Research

SyedHasnainNasirNaqvi

***/**, ***** *****, ****** Badi Masjid, Lucknow - 226003

Phone: +91-522-*******; Mobile: +91-941*******, +91-941*******

Email: h **************@*****.**.**, **********@**********.***, *******.******@*****.***

T echnical Assistance / Lab I nst ructor, R esearch &

D evelopment – Toxicology Department

Offering an impressive experience of over 25 years in Indian Institute of Toxicology Research .

P rofile

• H ighly accomplished Technical Assistant w ith significant experience acqui red over

t he years in diverse areas encompassing

Training & development of under-graduate and postgraduate students in practical

techniques in Biochemistry / Biotechnology / Proteomics / Cancer Chemoprevention

In vivo animal handling, induction of tumors in Swiss albino mouse by the application of

well known carcinogens and study of anti-tumorigenic and /or chemo preventive effect of

d ietary constituents

In vitro animal cell culture techniques

Flow cytometry for multiple parameters including immunophenotyping;

apoptotis / necrosis detection; cell cycle distribution, reactive oxygen species, Glutathione

content and mitochondrial membrane potential analysis

Tissue sectioning - Cryostat / m icrotome.

DNA, RNA isolation from mammalian blood, tissue, f luids and laboratory-cultured cells

Clinical / Biochemical tests - estimation of enzymatic parameters

• Hands on exposure to inhalation technology, biochemical testing, water and soil

a nalysis; experience in handling various l aboratory equipments l ike Flow Cytometry,

H PLC, GLC, RT-PCR, Western blotting, Spectrophotometer (nanodrop), Cryostat/microtome,

SDS-PAGE Electrophoresis, and routine lab instruments.

• Functional exposure to optimal utilization of available resources ; decisive team member,

able to build teams cohesion inspire and empower individuals to strive toward ever-higher

levels of achievement

• Versatile professional w ith a strong academic background, having several research works on

toxicology, publications & presentation, journals, experimental models, scientific reports, etc.

P rofessional Experience and Accomplishments

• I ndian I nstitute of Toxicology Research, Dec 1985 till date

(Formerly: Industrial Toxicology Research Center) Council of Scientific and Industrial

Research, New Delhi, Government of India

Technical Assistant - P roteomics Laboratory, Dec 2000 till date

• As an active Team Member, involved in studying & analyzing Hyaluronic acid or hyaluron as a

b iodegradable polymer and doxorubicin as an anti cancer drug

• Gained experience in conducting of 2 stage initiation promotion protocol in skin of mouse and

observed that Ninhydrin is acting as a stage I promoter in skin carcinogenesis system

• Team member of Proteomics breast cancer project, carcinogenic/tumerigenic to study the effect

of neem kernel powder on wistar rats in long term duration, plasma proteomics profiling of

auto mobile worker in Lucknow, and evaluated the potential of black tea polyphenol in animal

model

Technical Assistant - I nhalation Depa rtment, Dec 1985 – Dec 2000

• Gained exposure to inhalation techniques and as Team Member, assisted in the

standardization of conditions for experimental exposure of animals to vapors of Methyl

I socianate, Furfuraldehyde doped Kerosene Oil and the Biomass fuel smoke both in short term

and long term studies

• Learnt various facets of physiological (blood gasses) and path biochemical techniques,

h istopathology, histochemistry, and biochemical estimations techniques (general & MFO’s

enzymes) used for evaluation of pulmonary toxicity following exposure to air borne toxicants as

a routine laboratory work and analysis of hazardous waste of deferent parameters like metal

analysis, cyanide, total nitrogen, pesticides, polycyclic aromatic hydrocarbons in liquid and solid

hazardous waste and compilation of report

Academic and P rofessional Credentials

M. Sc.(Chemistry), 2012

B.Sc. (Physics, Chemistry, M aths), 1984

Lucknow University, Lucknow

Diploma in Foreign Affai rs & Diplomacy, 1985

Lucknow University, Lucknow

D iploma in Public Administration, 1990

Lucknow University, Lucknow

Computer P roficiency

MS-Word, MS-Excel, MS-PowerPoint

Research and Publications

Papers

• Inhalation toxicity of methyl isocynate: Biochemical and Cytological profile of

B ronchoalveolar lavage f luid in rats. Gupta GS, Kaw JL, Naqvi SHN, Dixit R, Ray PK, Journal

of Applied Toxicology (1991), 11(3): 157-60

• Tea polyphenols inhibit cyclooxygenase-2 expression and block activation of nuclear factor-

kappa B and Akt in diethylnitrosoamine induced lung tumors in Swiss mice. Roy P, Nigam N,

Singh M, Naqvi SHN, Srivastava S, George J and Shukla Y, Molecular Cancer, March 2009

(epub).

Abstract P resented

• Modulation of pulmonary mixed function oxidases in rats following inhalation of wood dust

smoke. Husnain MM, Naqvi SHN, Gupta GSD - presented at IX Society of Toxicology meeting

a t NIOH, Ahmadabad, Nov. 23-25, 1990

• Domestic fuel induced changes in trachea and lung: An experimental study. Srivastava AN,

Sinha KN, Akhtar S, Maheshwari P, Nath P, Sunderaraman S, Gupta GSD, Dutta KK, Naqvi

SHN. Presented at 2nd Congress of Toxicology in Developing countries at New Delhi, India

f rom Nov. 24-28, 1991

• Genotoxicity and Oxidative Stress Induced by Allethrin in Swiss Albino Mice. Amit Kumar

S rivastava, Syed H N Naqvi, Pramod Kumar Srivastava and Yogeshwer Shukla. Presented at

xxxvth annual conference of Environmental Mutagen Society of India (EMSI) and international

symposium on "environmental exposures to mutagens and carcinogen on human health" Feb.

4-6, 2011

T rainings & Semina rs Attended

• Attended seminar IX Society of Toxicology meeting at NIOH, at Ahmadabad, Nov. 23-25, 1990

• Attended seminar, 2nd Congress of Toxicology in Developing countries at New Delhi, India

f rom Nov. 24-28, 1991

• Attended xxxvth annual conference of Environmental Mutagen Society of India (EMSI) and

i nternational symposium on "environmental exposures to mutagens and carcinogen on human

health" Feb. 4-6, 2011

• Attended Abcam Seminar entitled “ Optimisation Techniques for Westarn Blotting (WB) &

I mmunohistochemistry organized by NuLife Supporting Science & abcam discover more in

CSIR-I ITR on 2nd, December, 2010 .

• Attended Abcam Seminar entitled “ Optimising Techniques for Immunohistochemistry (IHC)

Optimisation Techniques for Westarn Blotting (WB) organized by NuLife Supporting Science &

abcam discover more in CSIR-I ITR on 30th November, 2012 .

• Attended Symposium on Principles of Good Laboratory Practices, Organised by Society of

Toxicology (STOX), I ndia a t CSIR-Indian Institute of Toxicology Research, L ucknow on

December 4, 2012

• Attended X XXI I Annual Conference of Society of Toxicology (India) & International

Symposium on New Frontiers in Toxicology organized CSIR-Indian Institute of Toxicology

Research, L ucknow on December 5-7, 2012

Syed H asnain Nasir Naqvi

R eferences & Supporting Documents Available upon Request

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