Medical Development

May, ****

CURRICULUM VITAE

Personal information

Name: Zhongcai Ma, PhD

Address: ***** **** ***

Torrance, CA 90505

Telephone: 424-***-****

E-mail: ****@*****.***

Education

September, 1990~July, 1994

B.S. in Biochemisty, Department of Biochemistry and Biophysics, Wuhan

University, Wuhan, China

September, 1994~July, 1999

Ph.D. in Pharmacology, Department of Pharmacology, Shanghai Institute of

Materia Medica, Chinese Academy of Sciences, Shanghai,

China

Professional Experiences

October, 1999~September, 2001

Postdoctoral fellow, Department of Microbiology, Second Military Medical

University, Shanghai, China

October, 2001~September, 2004

Postdoctoral researcher, Department of Microbiology, Immunology, and

Molecular Genetics, University of California at Los Angeles (UCLA), CA

October, 2004~March, 2005

Staff Research Associate II, Department of Microbiology, Immunology, and

Molecular Genetics, UCLA, Los Angeles, CA

March ~ October, 2005

Staff Research Associate III, Department of Pathology and Laboratory

Medicine, UCLA, CA

November, 2005~December, 2007

Staff Research Associate IV, Department of Pediatrics, Los Angeles

Biomedical Research Institute at Harbor-UCLA Medical Center, UCLA,

Torrance, CA

January, 2008~ June, 2010

Assistant Researcher, Department of Pediatrics, Los Angeles Biomedical

Research Institute at Harbor-UCLA Medical Center, UCLA, Torrance, CA

July, 2010~September, 2011

Research Associate, Department of Neurology, Los Angeles Biomedical

Research Institute at Harbor-UCLA Medical Center, UCLA, Torrance, CA

October, 2011~ January, 2013

Research Scientist, NeuroInDx Inc., Signal Hill, CA

Awards and Grant Honors

. Di Ao Scholarship Award by Chinese Academy of Sciences, 1996, China

. Award of Excellent Academic Paper in Natural Science, 1996, Hubei, China

. Invited Reporter for Chinese Anti-Cancer News, Chinese Anti-Cancer

Association, 1996-1998

. Excellent Graduate Student Scholarship, Shanghai Institute of Materia

Medica, Chinese Academy of Sciences, 1997, China

. Principal Investigator of grant from Novartis, titled "Effect of Elidel

(pimecrolimus) on a murine model of cutaneous mastocytosis", 2007-2008

. Co-Principal Investigator of grant from Merk, titled "Effect of high-

dose montelukast on a murine model of urticaria pigmentosa", 2009

. Principal Investigator of Seed Grant, titled "Mechanism of USF-1 for the

regulation of 4G/5G polymorphism-dependent PAI-1 expression in mast

cells", Los Angeles Biomedical Research Institute at Harbor-UCLA Medical

Center, UCLA, 2009

. Principal Investigator of grant from SEPRACOR Inc., titled "Effect of

Formoterol Stereoisomers in the Fibrinolytic System in a Murine Asthma

Model", 2009-2010

. Co-Principal Investigator of Seed Grant, titled "Imbalanced expression

of pro versus anti-inflammatory cytokines is associated with development

of early wheezing disease in children born prematurely", Los Angeles

Biomedical Research Institute at Harbor-UCLA Medical Center, UCLA, 2010

Ad Roc Reviewer

Acta Pharmacologica Sinica

American Journal of Respiratory Cell and Molecular Biology

Annals of Allergy, Asthma & Immunology

Chinese Medical Journal

Clinical and Molecular Allergy

Cough

Journal of Allergy and Clinical Immunology

Journal of Asthma

Journal of Cellular Physiology

Journal of Dermatological Science

Pediatric Allergy and Immunology

Respiratory Medicine

Professional Memberships

American Association for the Advancement of Science (AAAS)

American Society for Biochemistry and Molecular Biology (ASBMB)

American Association of Allergy Asthma & Immunology (AAAAI)

Research Highlights

. NeuroInDx Inc., (10, 2011~01, 2013)

o Primary culture of neural progenitor cells from adult rat brains

o Immunohistochemistry and immunofluorescence staining of cultured cells

and human tissues

o Development and optimization of the protocols based on a new

microdissection instrument, cell and tissue acquistion system (CTAS).

o Development of the protocol of a novel concept based site-directed

cleavage of DNA sequences

o Development of a new technology and device for direct microcollection

of RNA from complex tissues

. Department of Neurology, Harbor-UCLA Medical Center, UCLA, (07, 2010 ~

09, 2011)

o Development of a specific cell-based assay to measure puromycin-

sensitive aminopeptidase (PSA) activity

o Identification of small molecular PSA activators as potential drugs to

treat neurodegenerative diseases through cell-based high throughput

screening (HTS)

o Maintenance of PSA overexpressing transgenic mice colonies and

genotyping

. Department of Pediatrics, Harbor-UCLA Medical Center, UCLA, (11, 2005~06,

2010)

o Study of molecular mechanisms for plasminogen activator inhibitor

(PAI)-1 expression in mast cells and its contribution to the

pathogenesis of asthma

o Study of effects of Zileuton, a 5-liposygenase inhibitor, and

montelukast in a murine model of allergic conjunctivitis

o Study of therapeutic effects and mechanisms of pimecrolimus on a

murine model of cutaneous mastocytosis

o Study of the effect of high-dose montelukast on a murine model of

cutaneous mastocytosis

o Study of effects of formoterol stereoisomers on mast cell-dependent

airway inflammation, airway remodeling and fibrinolytic system in a

murine asthma model

o Study of the imbalanced expression of pro versus anti-inflammatory

cytokines in the development of early wheezing disease in children

born prematurely

. Department of Pathology and Laboratory Medicine, UCLA (03~10, 2005)

o Study of expression and intracellular location of prostate specific

membrane antigen in human prostate cancer cell line COS-7 cells and

fibroblast-like PC3 cells

. Department of Microbiology, Immunology, and Molecular Genetics, UCLA (10,

2001~03, 2005)

o Probing functional residues in the C-terminal domain of the

Escherichia coli transcription factor NarL protein using alanine

scanning mutagenesis

o Study of binding activities of NarL to different NarL-associated

promoters

. Department of Microbiology, Second Military Medical University,

Shanghai, China (10, 1999~09, 2001)

o Screening small peptides that mimic the biological activities of brain-

derived neurotrophic factor from a phage-displayed conformation-

constrained random nonepeptides library

o Study of secreted endostain-mediated gene therapy of cancers

o Construction of the human interferon-( 2b phage display library

. Department of Pharmacology, Shanghai Institute of Materia Medica, Chinese

Academy of Sciences, Shanghai, China (09, 1994~07, 1999)

o Study of antitumor effects and mechanisms of a novel estrogen receptor

antagonist (-anordirn on Swarm rat chondrosarcoma model in vitro and

in vivo

o Study of in vitro and in vivo antiangiogenic activity and mechanisms

of (-anordirn

. Department of Biochemistry, Wuhan University, Wuhan, China (09, 1990~07,

1994)

o Establishment of an optimal method to purify hyaluronidase from blood-

sucking leeches

Major Skills and Expertise:

o Drug discovery through cell base high throughput screening;

o Bacterial and mammal cell culture, transduction, transfection and

associated in vitro assays;

o Primary culture of neural progenitor cells

o Immunohistochemistry and immunofluorescence staining, manipulating

fluorescence microscopy and image processing;

o ELISA;

o Recombinant DNA techniques, especially DNA extraction, vector

construction, site-directed mutagenesis and primer design and PCR;

o RNA extraction and analysis, including real-time RT-PCR;

o Allery/immunology;

o Biochemistry;

o Pharmacology;

o Oncology

o Establishment of animal models of human diseases to study functions of

compounds and genes in vivo, including allergic diseases and tumor

xenografts;

o Maintaining and breeding transgenic animal colonies and genotyping;

o Protein overexpression/purification, Western blot and functional

study;

o Gene expression/regulation, and signal transduction;

o Development and optimization of the protocol for a special assay;

o Serving as the PI or co-PI on several projects from industry and

institute;

o Preparing grant proposals to NIH (R01, R21, SBIR, etc), other funding

agencies, and industry;

o Preparing manuscripts for publication;

o Supervising a biomedical research laboratory;

o Working on multiple projects efficiently, effectively and

independently;

o Training postdoctoral researchers and physicians;

o Good at team work and strong written and verbal communication

Publications

Original Research Articles

1. Yang, T., Ma, Z. Purification of hyaluronidase from two kinds of blood-

sucking leeches. Acta Hydrobiol Sin, 1995; 19: 213-7

2. Ma, Z., He, G., Xu, B. Pathological changes of Swarm rat chondrosarcoma

treated with a-anordrin. In: Wang, W. (ed.), Advances in cancer research

in China, vol. 4. Guangzhou: Jinan University Press. 1998: 205-11

3. Ma, Z., He, G., Xu, B. The inhibitory effects of a-anordrin and other

drugs on the growth of Swarm rat chondrosarcoma. Chin J Clin Oncol,

1999; 26: 771-5

4. Ma, Z., He, G., Xu, B. Sterol hormone-dependent growth of Swarm rat

chondrosarcoma and its relation with the therapeutic effect of a-

anordrin. Chin Oncol, 1999; 9: 330-3

5. Ma, Z., Lou, L., Zhang, Z., Xu, B. Antiangiogenetic effect of (-

anordrin in vitro and in vivo. Acta Pharmacol Sin 2000; 21: 939-44

6. Ma, Z., He, G., Zhang, Z., Xu, B. Effects of (- anordrin and other drugs

on the growth of Swarm rat chondrosarcoma implanted into suberenal

capsule of mice. Tumor (Shanghai), 2000; 20: 237-40

7. Cao, M., Pan, W., Chen, Q., Ma, Z., Ni, Z., Wu, X., Cao, G., Qi, Z.

Construction of the eukaryotic expression vector expressing the fusion

protein of human endostain and IL-3 signal peptide. World Chin J

Digestol, 2000; 9: 43-6

8. Ma, Z., He, G. and Xu, B. a-Anordrin induced Swarm rat chondrosarcoma to

differentiate towards ossification. Chinese J Cancer, 2001; 20: 834-9

9. Ma, Z., Wu, X., Pan, W., Cao, M., Zhu, F., Qi, Z. Expression of brain-

derived neurotrophic factor receptor trkB on murine fibroblast NIH 3T3.

Life Sci Res, 2001; 5: 265-9

10. Wu, X., Pan, W., Ma, Z., Chen, Q., Wu, W., Cao, M., Qi, Z. Phage

display of human interferon (2b. Acad J Sec Military Med Uni, 2002; 23:

403-6

11. Ma, Z., Wu, X., Cao, M., Pan, W., Zhu, F., Chen, J., Qi, Z. Selection

of trkB-binding peptides from a phage-displayed random peptide

libraries. Science China (C), 2003; 46: 77-86

12. Maris, A.E., Kaczor-Grzeskowiak, M., Ma, Z., Kopka, M.L., Gunsalus,

R.P., Dickerson, R.E. Primary and secondary modes of DNA recognition by

the NarL two-component response regulator. Biochemistry 2005; 44:14538-

52

13. Ma, Z., Jhun, B., Oh, C.K. Upstream stimulating factor-1 mediates the E-

box-dependent transcriptional repression of the plasminogen activator

inhibitor-1 gene in human mast cells. FEBS Lett. 2007; 581: 4485-90

14. Ma, Z., Jhun, B., Jung, S.Y., Oh, C.K. Binding of USF-1 to the E-Box

regulates the 4G/5G polymorphism-dependent PAI-1 expression in mast

cells. J Allergy Clin Immunol 2008; 121:1006-12

15. Ma, Z., Tovar, J.P., Kwong, K.Y.C., Paek, D. Pimecrolimus induces

apoptosis of mast cells in a murine model of cutaneous mastocytosis. Int

Arch Allergy Immunol, 2010; 153:413-8

16. Ma, Z., Kwong, K.Y.C., Tovar, J.P., Peak, D. Cyclic adenosine

monophosphate induces plasminogen activator inhibitor-1 expression in

human mast cells Biochem Biochem Biophys Res Commun, 2010; 400:569-74.

17. Ren, G., Ma, Z., Hui, M., Kudo, L.C., Hui, K.S., Karsten, S.L. Cu, Zn-

superoxide dismutase 1 (SOD1) is a novel target of Puromycin-sensitive

aminopeptidase (PSA/NPEPPS): PSA/NPEPPS is a possible modifier of

amyotrophic lateral sclerosis. Mol Neurodegener. 2011; 6:29.

18. Kudo, L.C., Parfenova, L., Ren, G., Vi, N., Hui, M., Ma, Z., Lau, K.,

Gray, M., Bardag-Gorce, F., Wiedau-Pazos, M., Hui, K.S., Karsten, S.L.

Puromycin-sensitive aminopeptidase (PSA/NPEPPS) impedes development of

neuropathology in hPSA/TAU(P301L) double-transgenic mice. Hum Mol Genet.

2011; 20:1820-33.

19. Ren, G., Ma, Z., Hui, M., Kudo, L.C., Hui, K.S., Karsten, S.L.

Puromycin-sensitive aminopeptidase is involved in wild-type huntingtin

clearance. Mol Neurodegener. 2012; 7 Suppl 1:S5.

20. Kudo, L.C., Vi, N., Ma, Z., Fields, T., Avliyakulov, N.K., Haykinson,

M.J., Bragin, A., Karsten, S.L. Novel cell and tissue acquistion system

(CTAS): microdissection of live and frozen brain tissues. PLoS One.

2012; 7(7): e41564

21. Ma, Z., Kudo, L.C., Bragin, A., Karsten, S.L. Collection and

recultivation of individual adherent cells with a novel capillary-based

vacuum-assisted cell and tissue acquisition system (CTAS-Live). BMC

Biotechnol. 2013; submitted

22. Ma, Z., Kudo, L.C., Bragin, A., Damoiseaux, R, Karsten, S.L.

Identification of small molecule modulators of human puromycin-sensitive

aminopeptidase through a cell-based high-throughput screening. In

preparation

23. Ma, Z., Gunsalus, R.P. Alanine scanning mutational analysis of the

Escherichia coli NarL DNA binding domain reveals determinants for

recognition. In preparation

24. Ma, Z., Tovar, J.P., Kwong, K.Y.C. Effect of formoterol stereoisomers

on the airway inflammation and remodeling and fibrinolytic system in a

murine asthma model. In preparation

Reviews & Book Chapters

1. Ma, Z., Xu, B. Tumor multidrug resistance and its clinical reversal.

Chin J Prac Oncol, 1995; 10: 204-6

2. Ma, Z., Liu, M. Betulinic acid selectively inhibits growth of human

melanoma through induction of apoptosis. Fore Med Sci: Cancer Sec, 1996;

23: 190-191

3. Yao, T., Ma, Z., Xu, B. Lipid modifications of Ras protein. Chem Life,

1996; 16:9-11

4. Ma, Z., Xu, B. Progress in methods of screening farnesyltransferase

inhibitors as antitumor drugs. Fore Med Sci: Cancer Sec, 1997; 24: 25-8

5. Ma, Z., Xu, B. Progress in multidrug resistance-associated protein. Chem

Life, 1997; 17: 42-5

6. Ma, Z., Xu, B. A novel protein inducing tumor multidrug resistance: lung

resistance protein. Tumor (Shanghai), 1997; 17: 234-6

7. Ma, Z., Xu, B. Cellular signal transduction as new targets for

anticancer drugs. In: Jin, Z, Wang, Y and Su, D (ed.), Trends in

pharmacology (1997). Beijing: People's Medical Publishing House. 1997:

96-113

8. Ma, Z., Xu, B. Progress in the study of differentiation of chondrocytes

induced by retinoic acid. In: Jin, Z, Wang, Y and Su, D (ed.), Trends in

pharmacology (1999). Beijing: Science Press. 1999: 225-9

9. Ma Z., Wu, X., Qi, Z. Applications of phage-displayed peptide libraries

in the study of cellular signal transduction. Chem Life, 2001; 21:434-5

10. Ma, Z., Xu, B. Progress in clinical research of inhibitors of tumor

angiogenesis. Chin J Prac Oncol, 2001; 16:298-300

11. Ma, Z., Wu, X., Qi, Z. Relations of neurotrophin receptors with stage

and prognosis of neuroblastoma. Fore Med Sci: Cancer Sec, 2002; 29:74-6

12. Ma, Z., Pan, W., Qi, Z. Brain-derived neurotrophin factor and

Parkinson's disease. Chin J Neuroanatomy, 2002; 18:87-90

13. Xu, B., Ma, Z. Alkylating agents. In: Xu, S. (ed.), Chinese Clinical

Pharmacology. Peking: People's Medical Publishing House. 2003: 1309-1424

14. Xu, B., Ma, Z. Platinum antitumor drugs. In: Xu, S. (ed.), Chinese

Clinical Pharmacology. Peking: People's Medical Publishing House. 2003:

1509-1514

15. Ma, Z., Paek, D., Oh, CK. Mast cells-derived plasminogen activator

inhibitor-1 in the pathogenesis of asthma. In: Jung, JF and Scholz, L.T.

(ed.), Mast cells: roles, interactions and disorders. Nova Science

Publishers, Hauppauge, NY, 2008: 93-114.

16. Ma, Z., Paek, D., Oh, CK. Plasminogen activator inhibitor-1 and asthma:

role in the pathogenesis and molecular regulation. Clin Exp Allergy,

2009; 39:1136-44.

17. Ma Z., Jiao, Z. Mast cells as targets of pimecrolimus. Curr Pharm Des.

2011; 17:3823-9.

Selected Abstracts

1. Ma, Z., He, G., Xu, B. Hormone-dependent growth of Swarm rat

chondrosarcoma and its relation with the therapeutic effect of a-

anordrin. Acta Pharmacol Sin 1998;; 20: 93-94

2. Oh, CK, Ma, Z. Identification and characterization of cis-acting

regulatory elements of PAI-1 gene in human mast cells. J Allergy Clin

Immunol 2007; 119(1, Supplement 1):S51

3. Ma, Z., Oh, CK. The 4G/5G-genotype-specific regulation of plasminogen

activator inhibitor (PAI)-1 expression in human mast cells. J Allergy

Clin Immunol 2007; 119(1, Supplement 1):S52

4. Marzo, E., Ma, Z., Oh, CK. Effect of montelukast on collagen deposition

of the eyes in a murine model of chronic allergic eye disease. J Allergy

Clin Immunol 2007; 119(1, Supplement 1):S60

5. Jung, S.Y., Ma, Z., Oh, C.K. Effect of Formoterol Steroisomers on Mast

Cell-Dependent Airway Inflammation. J Allergy Clin Immunol 2008; 121(2,

Supplement 1): S112

6. Ma, Z., Jhun, B.S., Oh, C.K. USF-1 regulates the 4G/5G polymorphism-

dependent PAI-1 expression in human mast cells. J Allergy Clin Immunol

2008; 121(2, Supplement 1): S216

7. Ma, Z., Peak, D., Oh, C.K. The cAMP/PKA pathway positively regulates PAI-

1 expression in human mast cells. J Allergy Clin Immunol 2009; 123(2,

Supplement 1): S214

8. Tovar, J.P., Ma, Z. Elidel (Pimecrolimus) induces mast cell apoptosis in

a murine model of urticaria pigmentosa. J Allergy Clin Immunol 2009;

123:725

9. Ma, Z., Tovar, J.P., Kwong, K.Y.C. Effects of formoterol stereoisomers

on airway remodeling and fibrinolytic system in a murine asthma model. J

Allergy Clin Immunol 2010; 125(2, Supplement 1): AB45

Selected Conference Presentations

1. Ma, Z., He, G., Xu, B. Effects of (- anordrin and other drugs on the

growth of Swarm rat chondrosarcoma. The 6th National Conference of

Cancer Pharmacology and Chemotherapy of China. 1998; Taian, China. (Oral

presentation)

2. Ma, Z., He, G., Xu, B. Hormone-dependent growth of Swarm rat

chondrosarcome and its relation with the therapeutic effect of (-

anordrin. The Ninth China and Japan Joint Meeting on Pharmacology. 1998;

Shanghai, China. (Oral presentation)

3. Ma, Z., Xu, B. New progress in studies of inhibitors of tumor

angiogenesis. The 6th National Academic Conference of Oncology of China.

2002; Zhuhai, China

4. Cao, M., Pan, W., Chen, Q., Ma, Z., Ni, Z., Wu, X., Wu, W., Pan, X.,

Cao, G., Qi, Z. Construction of the eukaryotic expression vector

expressing the fusion protein of human endostain and IL-3 signal

peptide. The 13th National Academic Conference of Interferon and

Cytokines of China. 2002; Shanghai, China

5. Ma, Z., Oh, CK. The 4G/5G-genotype-specific regulation of plasminogen

activator inhibitor (PAI)-1 expression in human mast cells. The 63rd

AAAAI Annual Meeting. 2007; San Diego, CA

6. Oh, CK, Ma, Z. Identification and characterization of cis-acting

regulatory elements of PAI-1 gene in human mast cells. The 63rd AAAAI

Annual Meeting. 2007; San Diego, CA

7. Marzo, E., Ma, Z., Oh, CK. Effect of montelukast on collagen deposition

of the eyes in a murine model of chronic allergic eye disease. The 63rd

AAAAI Annual Meeting. 2007; San Diego, CA

8. Ma, Z., Jhun, B.S., Oh, C.K. USF-1 regulates the 4G/5G polymorphism-

dependent PAI-1 expression in human mast cells. The 64th AAAAI Annual

Meeting. 2008; Philadelphia, PA. (Oral presentation)

9. Jung, S.Y., Ma, Z., Oh, C.K. Effect of Formoterol Steroisomers on mast

cell-dependent airway inflammation. The 64th AAAAI Annual Meeting. 2008;

Philadelphia, PA.

10. Ma, Z., Paek, D., Oh, C.K. The cAMP/PKA pathway positively regulates

PAI-1 expression in human mast cells. The 65th Annual AAAAI Meeting,

2009; Washington, DC. (Oral presentation)

11. Tovar, J.P., Ma, Z. Elidel induces mast cells apoptosis in a murine

model of urticaria pigmentosa. The 65th Annual AAAAI Meeting, 2009;

Washington, DC. (Oral presentation)

12. Ma, Z., Tovar, J.P., Kwong, K.Y.C. Effect of formoterol stereoisomers

on the airway remodeling and fibrinolytic system in a murine asthma

model. The 66th Annual AAAAI Meeting, 2010; New Orleans, LA.

13. Ma, Z., Kudo, L., Karsten, S. Development of activators of puromycin-

sensitive aminopeptidase to treat CBD. International Research Symposium

of CurePSP, 2011; Crystal City, VA. (Oral presentation)

14. Karsten, S., Vi, N., Ma, Z. Fields, T., Avliyakulov, N., Haykinson, M.,

Bragin, A., Kudo, L. New low cost cell and tissue acquisition system

(CTAS): microdissection of live and frozen tissues. ABRF, 2012; Orlando,

FL.

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